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The map label for this gene is topB [H]

Identifier: 124265458

GI number: 124265458

Start: 286976

End: 289615

Strand: Direct

Name: topB [H]

Synonym: Mpe_A0265

Alternate gene names: 124265458

Gene position: 286976-289615 (Clockwise)

Preceding gene: 124265454

Following gene: 124265459

Centisome position: 7.1

GC content: 66.36

Gene sequence:

>2640_bases
TTGGCGTGCGGGACGGCGCTGCGATCGGGCGACAACGACATCGAGAACATGAGCAAAGCACTGGTCATCGCCGAGAAACC
TTCCGTCGCACAAGACATCGTGCGGGCCCTGACGCCCACGAGCGGCAAGTTCGAGAAGCACGACGATCACTTCGAGAACG
AGCACTACGTCGTCACCTCGGCGGTCGGCCACCTGGTCGAGATCAAGGCGCCCGAGGAGTACGACGTGAAGCGCGGCAAG
TGGAGCTTCGCCCACCTGCCGGTGATCCCGCCGCACTTCGATCTCGCACCGATCGACAAGGCCAAGAGCCGGCTCAACGC
GGTGGTCAAGCAGGTCAAGCGCAAGGACGTCGGCGAGCTCATCAATGCCTGTGACGCGGGACGCGAGGGCGAGCTGATCT
TCCGCCTCATCGTGCAGTACGCCGGCACCGAGAAGAAGCCGATCGACAAGCCGGTGCGCCGCCTGTGGCTTCAGAGCATG
ACGCCGCAGGCGATCCGCGACGGCTTCGAGCGCCTGCGCTCCGACGCCGATCTGCGCGGCCTGGCCGACGCCGCCCGCAG
CCGCAGCGAGGCCGACTGGCTGGTCGGCATCAACGGTACGCGAGCGATGACGGCCTTCAACTCGCGCGACGGCGGTTTCT
TCCTCACCACCGTCGGCCGGGTGCAGACGCCCACGCTCAGCATCGTGGTCGAGCGCGAGGAGAAGATCCGCAAGCACGTG
GCGCGCGACTACTGGGAGCTCAAGGCCACGTTCGCCGCCCAGGCCGGCGAGTACGAGGGCAAGTGGTTCGACCCGGCATG
GAAGAAGAACGACGATGCCGAGCGCCGCGCCGACCGGCTCTGGAACGAGGCCGATGCGCAGGCCATCGCGCAGGCGGTGC
GCGGCCAGCCGGCCACCGTGACCGAGGAATCCAAGCCCAGCACACAGAGCAGCCCTCAGCTCTATGACCTGACGCTGCTG
CAGCGCGAGGCCAACTCGCGCTTCGGCTTCTCGGCCAAGACCACGCTCAGCATCGCGCAGGCGCTGTACGAGAAGCACAA
GGTGCTGACCTACCCGCGGACCGACAGCAAGGCGCTGCCGGAAGACTACGTCGGCGTCGTGAAGCAGACGATGGAGATGC
TCGCCGCCGAGGATCTGCCCGGGCCGCTGAAGGCTCTGTCGGCGCACGCCCGCAAGGCGGTGAAGGAGGGCTACGTCAAG
CCCAACAAGCGCATCTTCGACAACGCCAAGGTCTCGGACCACTTCGCGATCATTCCGACCTTGCAGGCGCCCAAGAGCCT
GACCGAGGTGGAGGCCAAGCTCTACGACCTGGTGGTCAAGCGCTTCCTCGCGGTGTTCTACCCGCCGGCCGAGTTCCTGG
TCACGACGCGCATCTCCACCGTCAAGGCGGGCAGCGCTGAACATCGGTTCCAGACCCACGGCAAGGTGATGGTCAAGCCG
GGCTGGCTGGCCGTCTATGGCCGCGACGTGGAAGATGCGGATGCCACGCTGGTGCCGGTCGAGCCCGGCGAAGTGGTGCG
CACCGAGAGCGTTGACGTCAACGCGCTCAAGACCAAGCCGCCGGCACGCTACACCGAGGCCACGCTACTGTCGGCGATGG
AAAGCGCCGGCAAGCTGATCGATGACGACGAACTGCGCGAGGCGATGCAGGAGAAGGGGCTGGGCACGCCGGCCACCCGC
GCGCAGACCATCGAAGGCCTGATCCTCGAGAAGTACATGCACCGCGACGGCCGCGAGCTGATCCCCACCGCCAAGGCCTT
CCAGCTGATGACGCTGCTGCGCGGCCTGCAGGTCGAGGAGCTGACCCAGCCCGAGCTGACCGGCAACTGGGAGTACCAGC
TCGCCGAGATGGAGAAGGGCAAGCTCAGCCGCGACGCCTTCATGCGCGAAATCGCCGCGATGACGCAGAAGATCGTCGCC
AAGGCCAAGGAGTACGACCGCGACACCATCCCCGGCGACTATGCGACGCTGAAGACGCCCTGCCCGAAATGCGGCGGCGT
CGTGAAGGAGAACTACCGCCGCTTCACCTGTGCCGCGAAATCAGGCGACGCCGAGGGCTGCGGCTTCAGCATCAGCAAGA
TCCCCGGCGGGCGCAGCTTCGAGCTCGATGAAGTGGAGTCCTTCATCGCCGACAAGAAGATCGGCCCGCTCGAGGGCTTC
CGCTCCAAGGCCGGCTGGCCCTTCACGGCCGAACTCAAGCTGGCGTACGACGACGAGATCGCCAACTGGAAACTCGAGTT
CGACTTCGGCGACGACGCCAAGAAGGGCGAAGGCGACGGCACGCCGGTCGACTTCTCGGCGCAGCAGAGCCTGGGCGCCT
GCCCCAAGTGCAAGGGGCATGTCTACGAGCACGGCAGCAGCTACGTCTGCGAGCACGCGGTCGGCGCGCACGTCACCTGC
GACTTCAAGAGCGGCAAGATCATCCTGCAGCAGCCGGTGGCGCGCGAGCAGATGACCCGGCTGCTGCAGGAAGGCAAGAC
CGATCTGCTGGAGAACTTCGTGTCCAACAAGACGCGGCGCAAGTTCAAGGCGCGCCTGGCCTGGGACGCCAAGGAAGGCA
AGGTCGGCTTCGAGTTCGAGCCGAGGGCGGCCAAGGTGCCGGCCAAGAAGTCCGGGGTTCGCGCCGGCACCAAATCGTGA

Upstream 100 bases:

>100_bases
GTCGATTGCGTCATGTTCGAGTTTGGCTACATTGAATCATGGGTGCGCGCGCGTGAGCGCGTGCGCGCGAGAGCCCGGAT
TGTAGGGACGCCCCGCCGCG

Downstream 100 bases:

>100_bases
AGTCAACAGCTTGTACGACGCCGATGCTTTATGGGGGCGCAATGAAGGCGCTTAGTCTGGTCGCGACGTTGCTTGTGGCT
GGATGCGGCGGCGGCGGTGA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 879; Mature: 878

Protein sequence:

>879_residues
MACGTALRSGDNDIENMSKALVIAEKPSVAQDIVRALTPTSGKFEKHDDHFENEHYVVTSAVGHLVEIKAPEEYDVKRGK
WSFAHLPVIPPHFDLAPIDKAKSRLNAVVKQVKRKDVGELINACDAGREGELIFRLIVQYAGTEKKPIDKPVRRLWLQSM
TPQAIRDGFERLRSDADLRGLADAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREEKIRKHV
ARDYWELKATFAAQAGEYEGKWFDPAWKKNDDAERRADRLWNEADAQAIAQAVRGQPATVTEESKPSTQSSPQLYDLTLL
QREANSRFGFSAKTTLSIAQALYEKHKVLTYPRTDSKALPEDYVGVVKQTMEMLAAEDLPGPLKALSAHARKAVKEGYVK
PNKRIFDNAKVSDHFAIIPTLQAPKSLTEVEAKLYDLVVKRFLAVFYPPAEFLVTTRISTVKAGSAEHRFQTHGKVMVKP
GWLAVYGRDVEDADATLVPVEPGEVVRTESVDVNALKTKPPARYTEATLLSAMESAGKLIDDDELREAMQEKGLGTPATR
AQTIEGLILEKYMHRDGRELIPTAKAFQLMTLLRGLQVEELTQPELTGNWEYQLAEMEKGKLSRDAFMREIAAMTQKIVA
KAKEYDRDTIPGDYATLKTPCPKCGGVVKENYRRFTCAAKSGDAEGCGFSISKIPGGRSFELDEVESFIADKKIGPLEGF
RSKAGWPFTAELKLAYDDEIANWKLEFDFGDDAKKGEGDGTPVDFSAQQSLGACPKCKGHVYEHGSSYVCEHAVGAHVTC
DFKSGKIILQQPVAREQMTRLLQEGKTDLLENFVSNKTRRKFKARLAWDAKEGKVGFEFEPRAAKVPAKKSGVRAGTKS

Sequences:

>Translated_879_residues
MACGTALRSGDNDIENMSKALVIAEKPSVAQDIVRALTPTSGKFEKHDDHFENEHYVVTSAVGHLVEIKAPEEYDVKRGK
WSFAHLPVIPPHFDLAPIDKAKSRLNAVVKQVKRKDVGELINACDAGREGELIFRLIVQYAGTEKKPIDKPVRRLWLQSM
TPQAIRDGFERLRSDADLRGLADAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREEKIRKHV
ARDYWELKATFAAQAGEYEGKWFDPAWKKNDDAERRADRLWNEADAQAIAQAVRGQPATVTEESKPSTQSSPQLYDLTLL
QREANSRFGFSAKTTLSIAQALYEKHKVLTYPRTDSKALPEDYVGVVKQTMEMLAAEDLPGPLKALSAHARKAVKEGYVK
PNKRIFDNAKVSDHFAIIPTLQAPKSLTEVEAKLYDLVVKRFLAVFYPPAEFLVTTRISTVKAGSAEHRFQTHGKVMVKP
GWLAVYGRDVEDADATLVPVEPGEVVRTESVDVNALKTKPPARYTEATLLSAMESAGKLIDDDELREAMQEKGLGTPATR
AQTIEGLILEKYMHRDGRELIPTAKAFQLMTLLRGLQVEELTQPELTGNWEYQLAEMEKGKLSRDAFMREIAAMTQKIVA
KAKEYDRDTIPGDYATLKTPCPKCGGVVKENYRRFTCAAKSGDAEGCGFSISKIPGGRSFELDEVESFIADKKIGPLEGF
RSKAGWPFTAELKLAYDDEIANWKLEFDFGDDAKKGEGDGTPVDFSAQQSLGACPKCKGHVYEHGSSYVCEHAVGAHVTC
DFKSGKIILQQPVAREQMTRLLQEGKTDLLENFVSNKTRRKFKARLAWDAKEGKVGFEFEPRAAKVPAKKSGVRAGTKS
>Mature_878_residues
ACGTALRSGDNDIENMSKALVIAEKPSVAQDIVRALTPTSGKFEKHDDHFENEHYVVTSAVGHLVEIKAPEEYDVKRGKW
SFAHLPVIPPHFDLAPIDKAKSRLNAVVKQVKRKDVGELINACDAGREGELIFRLIVQYAGTEKKPIDKPVRRLWLQSMT
PQAIRDGFERLRSDADLRGLADAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREEKIRKHVA
RDYWELKATFAAQAGEYEGKWFDPAWKKNDDAERRADRLWNEADAQAIAQAVRGQPATVTEESKPSTQSSPQLYDLTLLQ
REANSRFGFSAKTTLSIAQALYEKHKVLTYPRTDSKALPEDYVGVVKQTMEMLAAEDLPGPLKALSAHARKAVKEGYVKP
NKRIFDNAKVSDHFAIIPTLQAPKSLTEVEAKLYDLVVKRFLAVFYPPAEFLVTTRISTVKAGSAEHRFQTHGKVMVKPG
WLAVYGRDVEDADATLVPVEPGEVVRTESVDVNALKTKPPARYTEATLLSAMESAGKLIDDDELREAMQEKGLGTPATRA
QTIEGLILEKYMHRDGRELIPTAKAFQLMTLLRGLQVEELTQPELTGNWEYQLAEMEKGKLSRDAFMREIAAMTQKIVAK
AKEYDRDTIPGDYATLKTPCPKCGGVVKENYRRFTCAAKSGDAEGCGFSISKIPGGRSFELDEVESFIADKKIGPLEGFR
SKAGWPFTAELKLAYDDEIANWKLEFDFGDDAKKGEGDGTPVDFSAQQSLGACPKCKGHVYEHGSSYVCEHAVGAHVTCD
FKSGKIILQQPVAREQMTRLLQEGKTDLLENFVSNKTRRKFKARLAWDAKEGKVGFEFEPRAAKVPAKKSGVRAGTKS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=666, Percent_Identity=27.1771771771772, Blast_Score=139, Evalue=2e-32,
Organism=Homo sapiens, GI4507635, Length=678, Percent_Identity=24.3362831858407, Blast_Score=112, Evalue=1e-24,
Organism=Escherichia coli, GI1788061, Length=637, Percent_Identity=33.4379905808477, Blast_Score=320, Evalue=3e-88,
Organism=Escherichia coli, GI1787529, Length=681, Percent_Identity=24.3759177679883, Blast_Score=129, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI32563869, Length=634, Percent_Identity=25.2365930599369, Blast_Score=131, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=647, Percent_Identity=24.8840803709428, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6323263, Length=676, Percent_Identity=24.112426035503, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585251, Length=671, Percent_Identity=25.7824143070045, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24640096, Length=665, Percent_Identity=22.8571428571429, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97636; Mature: 97505

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACGTALRSGDNDIENMSKALVIAEKPSVAQDIVRALTPTSGKFEKHDDHFENEHYVVTS
CCCCCCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
AVGHLVEIKAPEEYDVKRGKWSFAHLPVIPPHFDLAPIDKAKSRLNAVVKQVKRKDVGEL
CCCCEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
INACDAGREGELIFRLIVQYAGTEKKPIDKPVRRLWLQSMTPQAIRDGFERLRSDADLRG
HHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHH
LADAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREEKIRKHV
HHHHHHCCCCCCEEEECCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEECHHHHHHHHH
ARDYWELKATFAAQAGEYEGKWFDPAWKKNDDAERRADRLWNEADAQAIAQAVRGQPATV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
TEESKPSTQSSPQLYDLTLLQREANSRFGFSAKTTLSIAQALYEKHKVLTYPRTDSKALP
CCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCC
EDYVGVVKQTMEMLAAEDLPGPLKALSAHARKAVKEGYVKPNKRIFDNAKVSDHFAIIPT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCEEEEEC
LQAPKSLTEVEAKLYDLVVKRFLAVFYPPAEFLVTTRISTVKAGSAEHRFQTHGKVMVKP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHHHEECCCCHHHHHCCCEEEECC
GWLAVYGRDVEDADATLVPVEPGEVVRTESVDVNALKTKPPARYTEATLLSAMESAGKLI
CEEEEECCCCCCCCCEEEECCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCC
DDDELREAMQEKGLGTPATRAQTIEGLILEKYMHRDGRELIPTAKAFQLMTLLRGLQVEE
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHCCCHHH
LTQPELTGNWEYQLAEMEKGKLSRDAFMREIAAMTQKIVAKAKEYDRDTIPGDYATLKTP
CCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
CPKCGGVVKENYRRFTCAAKSGDAEGCGFSISKIPGGRSFELDEVESFIADKKIGPLEGF
CCCCCCHHHHCCCEEEEECCCCCCCCCCEEHCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
RSKAGWPFTAELKLAYDDEIANWKLEFDFGDDAKKGEGDGTPVDFSAQQSLGACPKCKGH
HHCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
VYEHGSSYVCEHAVGAHVTCDFKSGKIILQQPVAREQMTRLLQEGKTDLLENFVSNKTRR
HHHCCCCHHHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KFKARLAWDAKEGKVGFEFEPRAAKVPAKKSGVRAGTKS
HHHHHHEECCCCCCCCCEECCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure 
ACGTALRSGDNDIENMSKALVIAEKPSVAQDIVRALTPTSGKFEKHDDHFENEHYVVTS
CCCCCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
AVGHLVEIKAPEEYDVKRGKWSFAHLPVIPPHFDLAPIDKAKSRLNAVVKQVKRKDVGEL
CCCCEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
INACDAGREGELIFRLIVQYAGTEKKPIDKPVRRLWLQSMTPQAIRDGFERLRSDADLRG
HHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHH
LADAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREEKIRKHV
HHHHHHCCCCCCEEEECCCCEEEEEECCCCCCEEEEECCCCCCCEEEEEECHHHHHHHHH
ARDYWELKATFAAQAGEYEGKWFDPAWKKNDDAERRADRLWNEADAQAIAQAVRGQPATV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
TEESKPSTQSSPQLYDLTLLQREANSRFGFSAKTTLSIAQALYEKHKVLTYPRTDSKALP
CCCCCCCCCCCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCCC
EDYVGVVKQTMEMLAAEDLPGPLKALSAHARKAVKEGYVKPNKRIFDNAKVSDHFAIIPT
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCEEEEEC
LQAPKSLTEVEAKLYDLVVKRFLAVFYPPAEFLVTTRISTVKAGSAEHRFQTHGKVMVKP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEHHHHHEECCCCHHHHHCCCEEEECC
GWLAVYGRDVEDADATLVPVEPGEVVRTESVDVNALKTKPPARYTEATLLSAMESAGKLI
CEEEEECCCCCCCCCEEEECCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCC
DDDELREAMQEKGLGTPATRAQTIEGLILEKYMHRDGRELIPTAKAFQLMTLLRGLQVEE
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHCCCHHH
LTQPELTGNWEYQLAEMEKGKLSRDAFMREIAAMTQKIVAKAKEYDRDTIPGDYATLKTP
CCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
CPKCGGVVKENYRRFTCAAKSGDAEGCGFSISKIPGGRSFELDEVESFIADKKIGPLEGF
CCCCCCHHHHCCCEEEEECCCCCCCCCCEEHCCCCCCCCCCHHHHHHHHHCCCCCCHHHH
RSKAGWPFTAELKLAYDDEIANWKLEFDFGDDAKKGEGDGTPVDFSAQQSLGACPKCKGH
HHCCCCCEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCC
VYEHGSSYVCEHAVGAHVTCDFKSGKIILQQPVAREQMTRLLQEGKTDLLENFVSNKTRR
HHHCCCCHHHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KFKARLAWDAKEGKVGFEFEPRAAKVPAKKSGVRAGTKS
HHHHHHEECCCCCCCCCEECCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA