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The map label for this gene is ksgA

Identifier: 124265397

GI number: 124265397

Start: 220246

End: 221016

Strand: Direct

Name: ksgA

Synonym: Mpe_A0204

Alternate gene names: 124265397

Gene position: 220246-221016 (Clockwise)

Preceding gene: 124265396

Following gene: 124265401

Centisome position: 5.45

GC content: 70.04

Gene sequence:

>771_bases
GTGAAGCACATCGCCCGCAAGCGCTTCGGCCAGCATTTCCTGTCCGATGCGGCGGTGGTCGATGCGATCGTCGGCCTGAT
CGACCCGCGACCCGGGCAGGCCCTGGTCGAGATCGGCCCGGGTCTGGGCGCGATGACCGATCCGCTGGTGGCGCGCTGCG
AACACCTCACCGTCGTCGAGCTCGACCGCGACCTGGCCGCCCGGCTGCGGCGGCGGGCCGAGCTGCAGGTGATCGAGTCC
GACGTGCTGCGGGTGGACTTTGCCGCGCTGGCCATGGCCTCGGCGGGCCGGCTGCGGATCGTCGGGAATTTGCCCTACAA
CATCTCGACCCCCATCCTCTTTCATCTGCTGCCGGCCGCGGCGCAGGTCGAGGACCAGCACTTCATGCTGCAGAAGGAGG
TTGTCGAACGCATGGCCGCCGCCCCGTGCAGCAAGGACTACGGACGGCTCAGCGTGATGCTGCAGTGGCGCTATGACATC
GAGTCGGTGCTCGACGTGCCGCCCGAGGCTTTCGAACCCCCGCCGCGCGTGAACTCGGCGGTGGTCCGCATGCTGCCGTT
CCCGGCACCGCCTGCGGTCGACGCCGCGCTGCTCGGCGAGCTGGTGGCGACGGCGTTCTCGCAGCGTCGCAAGTTGCTGC
GACACACGCTGGGAAAGTGGCTCGACGCGCGCGAGTTCAGCGGGACCTTCGACACCCAGCGCCGCGCCGAGGAAGTGCCG
GTGGCCGATTACCTGGCCCTGGCCCTGGCGCTGACGCCCGGCGAGCGCTGA

Upstream 100 bases:

>100_bases
TACCAGGAGTGGGCGCGCGATGTGCGGGCCCGCGCCTACGTGGAGATGAGGGAGCCTCCGCAGTGAGCGTGAGGTCGGTG
ACAGTGGCCCCGCGGCAGGC

Downstream 100 bases:

>100_bases
AACGACAACGGACCCCTCAGGGCCCGTTGTCAGTGTCGACCGCGTCCGAGATCGTCAGGCCGCGACCAGCCACCCCGCCG
TGTCGAACGCGCTGCTCATG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKHIARKRFGQHFLSDAAVVDAIVGLIDPRPGQALVEIGPGLGAMTDPLVARCEHLTVVELDRDLAARLRRRAELQVIES
DVLRVDFAALAMASAGRLRIVGNLPYNISTPILFHLLPAAAQVEDQHFMLQKEVVERMAAAPCSKDYGRLSVMLQWRYDI
ESVLDVPPEAFEPPPRVNSAVVRMLPFPAPPAVDAALLGELVATAFSQRRKLLRHTLGKWLDAREFSGTFDTQRRAEEVP
VADYLALALALTPGER

Sequences:

>Translated_256_residues
MKHIARKRFGQHFLSDAAVVDAIVGLIDPRPGQALVEIGPGLGAMTDPLVARCEHLTVVELDRDLAARLRRRAELQVIES
DVLRVDFAALAMASAGRLRIVGNLPYNISTPILFHLLPAAAQVEDQHFMLQKEVVERMAAAPCSKDYGRLSVMLQWRYDI
ESVLDVPPEAFEPPPRVNSAVVRMLPFPAPPAVDAALLGELVATAFSQRRKLLRHTLGKWLDAREFSGTFDTQRRAEEVP
VADYLALALALTPGER
>Mature_256_residues
MKHIARKRFGQHFLSDAAVVDAIVGLIDPRPGQALVEIGPGLGAMTDPLVARCEHLTVVELDRDLAARLRRRAELQVIES
DVLRVDFAALAMASAGRLRIVGNLPYNISTPILFHLLPAAAQVEDQHFMLQKEVVERMAAAPCSKDYGRLSVMLQWRYDI
ESVLDVPPEAFEPPPRVNSAVVRMLPFPAPPAVDAALLGELVATAFSQRRKLLRHTLGKWLDAREFSGTFDTQRRAEEVP
VADYLALALALTPGER

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=211, Percent_Identity=33.175355450237, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI156415992, Length=236, Percent_Identity=30.0847457627119, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1786236, Length=253, Percent_Identity=49.4071146245059, Blast_Score=248, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI25146882, Length=211, Percent_Identity=32.7014218009479, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=27.3584905660377, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21358017, Length=247, Percent_Identity=29.9595141700405, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21357273, Length=238, Percent_Identity=30.672268907563, Blast_Score=87, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_METPP (A2SC77)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019401.1
- ProteinModelPortal:   A2SC77
- SMR:   A2SC77
- STRING:   A2SC77
- GeneID:   4783529
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0204
- NMPDR:   fig|279263.3.peg.249
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   A2SC77
- ProtClustDB:   PRK00274
- BioCyc:   MPET420662:MPE_A0204-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 28269; Mature: 28269

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 14-14 BINDING 39-39 BINDING 60-60 BINDING 81-81 BINDING 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIARKRFGQHFLSDAAVVDAIVGLIDPRPGQALVEIGPGLGAMTDPLVARCEHLTVVE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHCCCEEEE
LDRDLAARLRRRAELQVIESDVLRVDFAALAMASAGRLRIVGNLPYNISTPILFHLLPAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
AQVEDQHFMLQKEVVERMAAAPCSKDYGRLSVMLQWRYDIESVLDVPPEAFEPPPRVNSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHCCCCHHCCCCCCHHHH
VVRMLPFPAPPAVDAALLGELVATAFSQRRKLLRHTLGKWLDAREFSGTFDTQRRAEEVP
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
VADYLALALALTPGER
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKHIARKRFGQHFLSDAAVVDAIVGLIDPRPGQALVEIGPGLGAMTDPLVARCEHLTVVE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHCCCEEEE
LDRDLAARLRRRAELQVIESDVLRVDFAALAMASAGRLRIVGNLPYNISTPILFHLLPAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHH
AQVEDQHFMLQKEVVERMAAAPCSKDYGRLSVMLQWRYDIESVLDVPPEAFEPPPRVNSA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHCCCCHHCCCCCCHHHH
VVRMLPFPAPPAVDAALLGELVATAFSQRRKLLRHTLGKWLDAREFSGTFDTQRRAEEVP
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
VADYLALALALTPGER
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA