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The map label for this gene is gshB [H]

Identifier: 124265373

GI number: 124265373

Start: 195974

End: 196924

Strand: Direct

Name: gshB [H]

Synonym: Mpe_A0180

Alternate gene names: 124265373

Gene position: 195974-196924 (Clockwise)

Preceding gene: 124265372

Following gene: 124265374

Centisome position: 4.85

GC content: 70.03

Gene sequence:

>951_bases
ATGCAACTGCTCTTCATCGCCGATCCGCTGGACCACTTCAAGACCTACAAGGACACCACCTTCTCGATGATGCGCGAGGC
CGCGGCACGCGGCCACACGCTGCTGGCCTGCGAGCCCCGCCACGTGATGTGGGCGCGGGGCGGCGTGGTGAGCGCGCGCA
TGCAGGCGTTCACGCTCACCGGCGATGCGAAGGACTGGTACCGCACGGACGGCGCGGCGCAGCAGCGTCCGCTGAAGGAC
GTCGGCGCGGTGCTCATGCGCAAGGACCCGCCGTTCGACAGCGAGTACTTCTACGCCACCCACCTGCTGGAGCAGGCCGA
GCGCGAGGGCGCTCGCGTGTTCAATGCGCCGCGCGCGCTCCGTGACCACCCGGAGAAGCTGGCGATCCTCGAGTTTCCCG
AGCTGATCGGCCCCACGCTGGTCACGCGCGATGCCGACGACGTGCGTGCCTTCCATGCCGAGCACCGCGACATCATCCTG
AAGCCGCTCGACGGCATGGGCGGCATGGGCATCTTCCGGGTTCGCGAGGACGGATTGAACCTCGGCAGCATCACCGAGAC
GCTCAACAAGTTCGGCGCCCAGACCGTGATGGTGCAGCGCTTCCTGCCCGAGATCGCGCAGGGCGACAAGCGCATCCTGC
TGATCGGCGGCGAGCCGGTGCCGTTCAGCCTGGCGCGCATCCCGCAGGGCAGCGAGGTGCGCGGCAACCTGGCTGCCGGC
GGCAAGGGTGTGGCGCTGCCGCTCAGCGCGCGCGACCGCGAGATCGCCGAGACGCTCGGCCCGGTGCTGGCCGCGCGCGG
CCTGCTGCTGGTGGGGCTGGACGTGATCGGCGACTGCCTGACCGAGATCAACGTCACCAGCCCGACCGGCTTCCAGGAGA
TCACGCAGCAGACCGGCTTCGACGTGGCCCGGCGCTTCGTCGACGCGCTCGAGGCCGCGCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CGCGGGCATCGGCGCGGCCTTCTGGGGCGTGGTCGCCGGCACGATCGCTCTCGTTGTGCAACAGTGGCGCAAGACCTGAG
CCTTCCGAAGGACGCTTTCG

Downstream 100 bases:

>100_bases
CCGCCTGCGCTTACCATTCGCGCTGCGGCGCCCGACATTCCGGTCGGGCGCCGCCTTTCATTCATGGCCCTGCTGACCCT
TTCCAACGCCCACCTGGCCT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MQLLFIADPLDHFKTYKDTTFSMMREAAARGHTLLACEPRHVMWARGGVVSARMQAFTLTGDAKDWYRTDGAAQQRPLKD
VGAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNAPRALRDHPEKLAILEFPELIGPTLVTRDADDVRAFHAEHRDIIL
KPLDGMGGMGIFRVREDGLNLGSITETLNKFGAQTVMVQRFLPEIAQGDKRILLIGGEPVPFSLARIPQGSEVRGNLAAG
GKGVALPLSARDREIAETLGPVLAARGLLLVGLDVIGDCLTEINVTSPTGFQEITQQTGFDVARRFVDALEAALAA

Sequences:

>Translated_316_residues
MQLLFIADPLDHFKTYKDTTFSMMREAAARGHTLLACEPRHVMWARGGVVSARMQAFTLTGDAKDWYRTDGAAQQRPLKD
VGAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNAPRALRDHPEKLAILEFPELIGPTLVTRDADDVRAFHAEHRDIIL
KPLDGMGGMGIFRVREDGLNLGSITETLNKFGAQTVMVQRFLPEIAQGDKRILLIGGEPVPFSLARIPQGSEVRGNLAAG
GKGVALPLSARDREIAETLGPVLAARGLLLVGLDVIGDCLTEINVTSPTGFQEITQQTGFDVARRFVDALEAALAA
>Mature_316_residues
MQLLFIADPLDHFKTYKDTTFSMMREAAARGHTLLACEPRHVMWARGGVVSARMQAFTLTGDAKDWYRTDGAAQQRPLKD
VGAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNAPRALRDHPEKLAILEFPELIGPTLVTRDADDVRAFHAEHRDIIL
KPLDGMGGMGIFRVREDGLNLGSITETLNKFGAQTVMVQRFLPEIAQGDKRILLIGGEPVPFSLARIPQGSEVRGNLAAG
GKGVALPLSARDREIAETLGPVLAARGLLLVGLDVIGDCLTEINVTSPTGFQEITQQTGFDVARRFVDALEAALAA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=314, Percent_Identity=47.1337579617834, Blast_Score=288, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34617; Mature: 34617

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLFIADPLDHFKTYKDTTFSMMREAAARGHTLLACEPRHVMWARGGVVSARMQAFTLT
CEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCEEEEEEEEEE
GDAKDWYRTDGAAQQRPLKDVGAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNAPRAL
CCCHHHHHCCCCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCHHH
RDHPEKLAILEFPELIGPTLVTRDADDVRAFHAEHRDIILKPLDGMGGMGIFRVREDGLN
HCCCHHEEEECCHHHHCCEEEECCCHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCC
LGSITETLNKFGAQTVMVQRFLPEIAQGDKRILLIGGEPVPFSLARIPQGSEVRGNLAAG
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCCC
GKGVALPLSARDREIAETLGPVLAARGLLLVGLDVIGDCLTEINVTSPTGFQEITQQTGF
CCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCH
DVARRFVDALEAALAA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLLFIADPLDHFKTYKDTTFSMMREAAARGHTLLACEPRHVMWARGGVVSARMQAFTLT
CEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCEEEEEEEEEE
GDAKDWYRTDGAAQQRPLKDVGAVLMRKDPPFDSEYFYATHLLEQAEREGARVFNAPRAL
CCCHHHHHCCCCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCHHH
RDHPEKLAILEFPELIGPTLVTRDADDVRAFHAEHRDIILKPLDGMGGMGIFRVREDGLN
HCCCHHEEEECCHHHHCCEEEECCCHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCC
LGSITETLNKFGAQTVMVQRFLPEIAQGDKRILLIGGEPVPFSLARIPQGSEVRGNLAAG
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCCC
GKGVALPLSARDREIAETLGPVLAARGLLLVGLDVIGDCLTEINVTSPTGFQEITQQTGF
CCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCH
DVARRFVDALEAALAA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]