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The map label for this gene is hisS [H]

Identifier: 124027827

GI number: 124027827

Start: 962648

End: 963940

Strand: Reverse

Name: hisS [H]

Synonym: Hbut_0954

Alternate gene names: 124027827

Gene position: 963940-962648 (Counterclockwise)

Preceding gene: 124027828

Following gene: 124027826

Centisome position: 57.82

GC content: 47.56

Gene sequence:

>1293_bases
ATGCCGGCGAGAATACCTCTCGAACCACTCAGGGGATTTAGAGACATACTAGAACCAGACTCTAAAGCACTAACACTACT
TGCGCAAAAATTTGCAAAACTAGCAGAACTCCACGGCTACGAGGAAGTCATTCCACCAACACTGGAGAGGTTCGAACTCT
TTGCCCTCAAATCCGGCGAGGAGATAAGAAGAACAATGTATGTTTTCCGGGACAAAGCTGGCCGCGAAGTAGCGTTGAGA
CCCGAAGCAACAGCTAGTATAGCGAGAATATACCTTAAACACCTACGAGCAAGACCAAAACCAATCAAGCTCTACTACAT
AGTTAACTGTTTCCGATACGAAGAGCCGCAGAGGGCAAGATACCGTGAATTCTGGCAGGCTGGAGTCGAATACCTTGGCG
CTGAGGGCGTGGAGGGTGACTTCGACCCAATAAAGCTCCTAGTAGCGTTCTACAACAGCATCGGCATGCTGGATGACATA
AGTCTGAAATTAGGTACAACCAAGCTTTACAGGATACTATTCGGGAAGTACGGTGTTGATGAGGAGTTACAAGACCACAT
ACTACACCTAATGGATAAGAAAATGTATAATGAAGCACTAGCCCTTGTCGAAGAGAAGGTTGGCCGAGACCTTGCAAGCT
TACTGGAAGAGCTATGGAGGAATCCCGCAGACCTCGAAAACGCTAGGGAGGCAGTTGGAGACCCAGAGGCTGCTAGAGCT
GTAGAAGAGCTACAAACCATAGCAAGCCTAATAAAAACCTACAACACCCGCATTGAAGTCCGAGTGAACCTATCGTTTGC
GCGAGGCCTAGCTTACTATACCGGCATCATATACGAGGTAGAGACAAAAGCTATTCCCGTAAGTATAGCTGGCGGAGGGA
GATATGATAATCTCATAGAGCTTTATGGGGGCGAGCACGTACCCGGGACAGGTTTCGCTATAGGCCTAGACCGTACATTA
GCAGCACTCAAGGAGCTAAGCAAGCTGGATAAGCTGCTAGCAAAACCTGTCAGGGCAGCCATAGTGGCTCTGAGTACTGA
GGTTCTTCCCTACGCAGCAGAGATCCAGGATGAACTTGTAAGAATGAATATCGCCTCTACAATGCTCGTCGAGCAAAAAC
TCCATAAGGCGCTGGCATGGATAGCAAGACAAGGTTATAGGTACGCCGTGATAATCGGCAAGAAGGAGCAGGAGGAGCAA
ACGCTAACCATTAGAGACCTTGAAAAACATTTGCAGAAGACAATAGAGTATTCAATGCTAAAGGAACTGGAAGCCCTCGA
TAACATATTCTAG

Upstream 100 bases:

>100_bases
AACAACTGGGAACCAATCCTAGCCATAGTCGATAGATACGGCGACATAACGTACTATAGTGTGACGCTTTTCCACCCACA
ACCAGCGAGGACGATGCAGC

Downstream 100 bases:

>100_bases
CCCTCCCAGGGTAGGAACAGTGTAATAGTATACAGCATTAGTGGGGAAGCGTCAGGTACTTCACAGGACAGCCTATAGCG
AGGCGGGGAGCGGTATTGGA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPARIPLEPLRGFRDILEPDSKALTLLAQKFAKLAELHGYEEVIPPTLERFELFALKSGEEIRRTMYVFRDKAGREVALR
PEATASIARIYLKHLRARPKPIKLYYIVNCFRYEEPQRARYREFWQAGVEYLGAEGVEGDFDPIKLLVAFYNSIGMLDDI
SLKLGTTKLYRILFGKYGVDEELQDHILHLMDKKMYNEALALVEEKVGRDLASLLEELWRNPADLENAREAVGDPEAARA
VEELQTIASLIKTYNTRIEVRVNLSFARGLAYYTGIIYEVETKAIPVSIAGGGRYDNLIELYGGEHVPGTGFAIGLDRTL
AALKELSKLDKLLAKPVRAAIVALSTEVLPYAAEIQDELVRMNIASTMLVEQKLHKALAWIARQGYRYAVIIGKKEQEEQ
TLTIRDLEKHLQKTIEYSMLKELEALDNIF

Sequences:

>Translated_430_residues
MPARIPLEPLRGFRDILEPDSKALTLLAQKFAKLAELHGYEEVIPPTLERFELFALKSGEEIRRTMYVFRDKAGREVALR
PEATASIARIYLKHLRARPKPIKLYYIVNCFRYEEPQRARYREFWQAGVEYLGAEGVEGDFDPIKLLVAFYNSIGMLDDI
SLKLGTTKLYRILFGKYGVDEELQDHILHLMDKKMYNEALALVEEKVGRDLASLLEELWRNPADLENAREAVGDPEAARA
VEELQTIASLIKTYNTRIEVRVNLSFARGLAYYTGIIYEVETKAIPVSIAGGGRYDNLIELYGGEHVPGTGFAIGLDRTL
AALKELSKLDKLLAKPVRAAIVALSTEVLPYAAEIQDELVRMNIASTMLVEQKLHKALAWIARQGYRYAVIIGKKEQEEQ
TLTIRDLEKHLQKTIEYSMLKELEALDNIF
>Mature_429_residues
PARIPLEPLRGFRDILEPDSKALTLLAQKFAKLAELHGYEEVIPPTLERFELFALKSGEEIRRTMYVFRDKAGREVALRP
EATASIARIYLKHLRARPKPIKLYYIVNCFRYEEPQRARYREFWQAGVEYLGAEGVEGDFDPIKLLVAFYNSIGMLDDIS
LKLGTTKLYRILFGKYGVDEELQDHILHLMDKKMYNEALALVEEKVGRDLASLLEELWRNPADLENAREAVGDPEAARAV
EELQTIASLIKTYNTRIEVRVNLSFARGLAYYTGIIYEVETKAIPVSIAGGGRYDNLIELYGGEHVPGTGFAIGLDRTLA
ALKELSKLDKLLAKPVRAAIVALSTEVLPYAAEIQDELVRMNIASTMLVEQKLHKALAWIARQGYRYAVIIGKKEQEEQT
LTIRDLEKHLQKTIEYSMLKELEALDNIF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=415, Percent_Identity=25.7831325301205, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI15029520, Length=447, Percent_Identity=26.8456375838926, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788861, Length=425, Percent_Identity=25.8823529411765, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325290, Length=462, Percent_Identity=24.8917748917749, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24643061, Length=350, Percent_Identity=27.1428571428571, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24643059, Length=330, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 48936; Mature: 48805

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARIPLEPLRGFRDILEPDSKALTLLAQKFAKLAELHGYEEVIPPTLERFELFALKSGE
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCH
EIRRTMYVFRDKAGREVALRPEATASIARIYLKHLRARPKPIKLYYIVNCFRYEEPQRAR
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHCCCHHHHH
YREFWQAGVEYLGAEGVEGDFDPIKLLVAFYNSIGMLDDISLKLGTTKLYRILFGKYGVD
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCC
EELQDHILHLMDKKMYNEALALVEEKVGRDLASLLEELWRNPADLENAREAVGDPEAARA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHH
VEELQTIASLIKTYNTRIEVRVNLSFARGLAYYTGIIYEVETKAIPVSIAGGGRYDNLIE
HHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHEEEEEECCEEEEEEECCCCCHHHHH
LYGGEHVPGTGFAIGLDRTLAALKELSKLDKLLAKPVRAAIVALSTEVLPYAAEIQDELV
HCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMNIASTMLVEQKLHKALAWIARQGYRYAVIIGKKEQEEQTLTIRDLEKHLQKTIEYSML
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
KELEALDNIF
HHHHHHHCCC
>Mature Secondary Structure 
PARIPLEPLRGFRDILEPDSKALTLLAQKFAKLAELHGYEEVIPPTLERFELFALKSGE
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCH
EIRRTMYVFRDKAGREVALRPEATASIARIYLKHLRARPKPIKLYYIVNCFRYEEPQRAR
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHCCCHHHHH
YREFWQAGVEYLGAEGVEGDFDPIKLLVAFYNSIGMLDDISLKLGTTKLYRILFGKYGVD
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCC
EELQDHILHLMDKKMYNEALALVEEKVGRDLASLLEELWRNPADLENAREAVGDPEAARA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHH
VEELQTIASLIKTYNTRIEVRVNLSFARGLAYYTGIIYEVETKAIPVSIAGGGRYDNLIE
HHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHHHHEEEEEECCEEEEEEECCCCCHHHHH
LYGGEHVPGTGFAIGLDRTLAALKELSKLDKLLAKPVRAAIVALSTEVLPYAAEIQDELV
HCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMNIASTMLVEQKLHKALAWIARQGYRYAVIIGKKEQEEQTLTIRDLEKHLQKTIEYSML
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
KELEALDNIF
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA