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The map label for this gene is gdhA [H]

Identifier: 124027737

GI number: 124027737

Start: 863960

End: 865228

Strand: Reverse

Name: gdhA [H]

Synonym: Hbut_0861

Alternate gene names: 124027737

Gene position: 865228-863960 (Counterclockwise)

Preceding gene: 124027739

Following gene: 124027736

Centisome position: 51.9

GC content: 51.93

Gene sequence:

>1269_bases
ATGGCTACAGACTCCCGCCGCCTCCTCGAAGAGGCCGAGAAGATCCTACACCGTGCGGTTGTCGAGCTTCTAGGTTACGG
CGAGGATGTTTATGAGGCTCTAAGGCATCCTGAGAGGGTTATCCAGGTTAAGATCCCCGTACGTATGGATGATGGTACAG
TTAAGGTCTTTATTGGTTGGAGGAGCCAGCACAATAGCGCTCTAGGCCCATACAAGGGCGGTATACGTTACCACCCAGAG
ACTACCAAGGAGGAGGTAATAGCTCTAAGCATGATGATGACCTGGAAGAACGCGTTGGCGGGCCTACCATATGGCGGTGG
TAAAGGTGGCATTCGCGTAGACCCGCACAAGCTCTCGGCACGCGAGCTTGAGCAGCTATCCCGTGGCTACATAAGGATGA
TATACAAGTACATTGGCCCAGACCACGACATACCAGCACCAGATGTCTATACAAACCCACAGATAATGGCCTGGATGATG
GACGAGTACTACAGAGTGACTGGTCAGCACCACTTTGGCGTCATAACTGGCAAGCCAAAGATACTAGGCGGCCTCGAGAC
CCGTGTAATAGCCACAGGTTTCGGTACAGCTGTTGCTGCTAGAGAGGTTGCCAAGAAAGTTTGGGGCAGCGTTGAGGGCA
AGAGGGTGGCTGTACAAGGCTATGGCAATGCTGGCTACTACGCGGCTAAGTTCCTCCACGAGATGGGCGCGATAATAGTA
GCTGTAAGCGACAGCAAGGGCGGCATTTACAGCAGCAAGGGCCTCAACCCAGACGAGGTTAAGGAGGTTAAGAGCAAGAC
CGGCTCGGTAATCAACTATGAGAAGGCAGAGAGGAAGATAACTAATGAGGAGCTATTAGAGCTAGATGTAGACATACTAG
TACCCGCAGCAATCGAGAATGTGATAACCGAGGAGAACGCTTCGAGGATCAAGGCTAAGATAATTGCCGAGGCAGCCAAC
GGCCCAACCACAGCGGAAGCCGACAAGATCCTTGCACAGCGCGGCATCATAGTAATACCGGACATTCTAGCCAATGCTGG
TGGTGTAATAATGAGCCACATTGAGTGGGTCAACAACAGGATGGGTGGCTGGATAACAGAGGAGGAGGCCAAGAGGGCAC
TAGAAAACAAGATGGTCCACAACACATTAGCAGTCTGGGACTTCTGGCACAAGGAGTTCGACACACAGAAGCATCCAATG
AGGGATGCTGCCTACGCCTTAGCAGTAAAGCGTGTAATTGAGGCTATGAAGCTACGTGGTCTCCTCTAA

Upstream 100 bases:

>100_bases
ATTGAATCGCTCCCTCTAAATAAACACTGTGAAAAGAGAACGTGCACGATAAATAGCTCAGAGAACTGTCTATACAAACT
GGAGCAGCGGGGGCTCAATC

Downstream 100 bases:

>100_bases
CCCACACAAACCAGTCTGTTTTTCTCCATAACTTTACTACTGCTCGTACCCATCCTATATAGCATAATTTGATATGATTT
GTATGTGATAGACTAGCTGT

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MATDSRRLLEEAEKILHRAVVELLGYGEDVYEALRHPERVIQVKIPVRMDDGTVKVFIGWRSQHNSALGPYKGGIRYHPE
TTKEEVIALSMMMTWKNALAGLPYGGGKGGIRVDPHKLSARELEQLSRGYIRMIYKYIGPDHDIPAPDVYTNPQIMAWMM
DEYYRVTGQHHFGVITGKPKILGGLETRVIATGFGTAVAAREVAKKVWGSVEGKRVAVQGYGNAGYYAAKFLHEMGAIIV
AVSDSKGGIYSSKGLNPDEVKEVKSKTGSVINYEKAERKITNEELLELDVDILVPAAIENVITEENASRIKAKIIAEAAN
GPTTAEADKILAQRGIIVIPDILANAGGVIMSHIEWVNNRMGGWITEEEAKRALENKMVHNTLAVWDFWHKEFDTQKHPM
RDAAYALAVKRVIEAMKLRGLL

Sequences:

>Translated_422_residues
MATDSRRLLEEAEKILHRAVVELLGYGEDVYEALRHPERVIQVKIPVRMDDGTVKVFIGWRSQHNSALGPYKGGIRYHPE
TTKEEVIALSMMMTWKNALAGLPYGGGKGGIRVDPHKLSARELEQLSRGYIRMIYKYIGPDHDIPAPDVYTNPQIMAWMM
DEYYRVTGQHHFGVITGKPKILGGLETRVIATGFGTAVAAREVAKKVWGSVEGKRVAVQGYGNAGYYAAKFLHEMGAIIV
AVSDSKGGIYSSKGLNPDEVKEVKSKTGSVINYEKAERKITNEELLELDVDILVPAAIENVITEENASRIKAKIIAEAAN
GPTTAEADKILAQRGIIVIPDILANAGGVIMSHIEWVNNRMGGWITEEEAKRALENKMVHNTLAVWDFWHKEFDTQKHPM
RDAAYALAVKRVIEAMKLRGLL
>Mature_421_residues
ATDSRRLLEEAEKILHRAVVELLGYGEDVYEALRHPERVIQVKIPVRMDDGTVKVFIGWRSQHNSALGPYKGGIRYHPET
TKEEVIALSMMMTWKNALAGLPYGGGKGGIRVDPHKLSARELEQLSRGYIRMIYKYIGPDHDIPAPDVYTNPQIMAWMMD
EYYRVTGQHHFGVITGKPKILGGLETRVIATGFGTAVAAREVAKKVWGSVEGKRVAVQGYGNAGYYAAKFLHEMGAIIVA
VSDSKGGIYSSKGLNPDEVKEVKSKTGSVINYEKAERKITNEELLELDVDILVPAAIENVITEENASRIKAKIIAEAANG
PTTAEADKILAQRGIIVIPDILANAGGVIMSHIEWVNNRMGGWITEEEAKRALENKMVHNTLAVWDFWHKEFDTQKHPMR
DAAYALAVKRVIEAMKLRGLL

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=333, Percent_Identity=40.8408408408408, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI4885281, Length=333, Percent_Identity=40.8408408408408, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1788059, Length=354, Percent_Identity=35.5932203389831, Blast_Score=187, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17544676, Length=352, Percent_Identity=37.5, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6319256, Length=390, Percent_Identity=32.5641025641026, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324951, Length=386, Percent_Identity=31.6062176165803, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI45549226, Length=338, Percent_Identity=39.3491124260355, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI45553475, Length=338, Percent_Identity=39.3491124260355, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24649283, Length=365, Percent_Identity=37.5342465753425, Blast_Score=240, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24649460, Length=283, Percent_Identity=39.9293286219081, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 46869; Mature: 46737

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDSRRLLEEAEKILHRAVVELLGYGEDVYEALRHPERVIQVKIPVRMDDGTVKVFIGW
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEEECCCEEEEEEEE
RSQHNSALGPYKGGIRYHPETTKEEVIALSMMMTWKNALAGLPYGGGKGGIRVDPHKLSA
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHCCH
RELEQLSRGYIRMIYKYIGPDHDIPAPDVYTNPQIMAWMMDEYYRVTGQHHFGVITGKPK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCH
ILGGLETRVIATGFGTAVAAREVAKKVWGSVEGKRVAVQGYGNAGYYAAKFLHEMGAIIV
HCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEE
AVSDSKGGIYSSKGLNPDEVKEVKSKTGSVINYEKAERKITNEELLELDVDILVPAAIEN
EEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHCCHHHHHHHHHHHCCHHHHHH
VITEENASRIKAKIIAEAANGPTTAEADKILAQRGIIVIPDILANAGGVIMSHIEWVNNR
HHHCCCHHHHHHHHEEECCCCCCCHHHHHHHHHCCCEEECHHHCCCCHHHHHHHHHHHCC
MGGWITEEEAKRALENKMVHNTLAVWDFWHKEFDTQKHPMRDAAYALAVKRVIEAMKLRG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
LL
CC
>Mature Secondary Structure 
ATDSRRLLEEAEKILHRAVVELLGYGEDVYEALRHPERVIQVKIPVRMDDGTVKVFIGW
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEEEEECCCEEEEEEEE
RSQHNSALGPYKGGIRYHPETTKEEVIALSMMMTWKNALAGLPYGGGKGGIRVDPHKLSA
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHCCH
RELEQLSRGYIRMIYKYIGPDHDIPAPDVYTNPQIMAWMMDEYYRVTGQHHFGVITGKPK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCH
ILGGLETRVIATGFGTAVAAREVAKKVWGSVEGKRVAVQGYGNAGYYAAKFLHEMGAIIV
HCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEE
AVSDSKGGIYSSKGLNPDEVKEVKSKTGSVINYEKAERKITNEELLELDVDILVPAAIEN
EEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHCCHHHHHHHHHHHCCHHHHHH
VITEENASRIKAKIIAEAANGPTTAEADKILAQRGIIVIPDILANAGGVIMSHIEWVNNR
HHHCCCHHHHHHHHEEECCCCCCCHHHHHHHHHCCCEEECHHHCCCCHHHHHHHHHHHCC
MGGWITEEEAKRALENKMVHNTLAVWDFWHKEFDTQKHPMRDAAYALAVKRVIEAMKLRG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
LL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]