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The map label for this gene is ppnK [H]

Identifier: 124027600

GI number: 124027600

Start: 707692

End: 708504

Strand: Reverse

Name: ppnK [H]

Synonym: Hbut_0721

Alternate gene names: 124027600

Gene position: 708504-707692 (Counterclockwise)

Preceding gene: 124027606

Following gene: 124027598

Centisome position: 42.5

GC content: 67.4

Gene sequence:

>813_bases
TTGCTCCGCAGGGTCGCCATATACGCGAGGCCCGACCGTAGGGAGGCGCTGGAGCTGGCTAGAGAGGCTTACCGCCGCTT
GAGGGAGGCGGGGGCGGAGGCCTTCTACGATGCCTCGATAGCCGGGCTGGTCGGGGGCCCCAGCACCGACGTGCGATTCG
ACGACGTGGACGGCGTCGTGGTGATTGGCGGTGACGGTACGCTGCTCCGGCTCCTCCAGCTCCTGGGCTCCAAGACGCCG
GTGCTCCACCTGGTGAGGCTCGGCCGGAAGGCGTTCTTCTTCGACGAGGAGCCCGGAGAGGCCCTCGACAGGATCGGGGA
CTTCGTTGCGGGGCACTTTGAGGTCGAGCAGCGGGTGAGGCTCCACGTTGAGGTGCAGGGTGTGCCGGTCTACGCTTTCA
ACGAGGCCGCTGTGCTGGGGTCGGGCTCCAAGATACTGGTTGTGAGGGTTCGGGCCGGCGACGACACGGTGTATGAGAGG
CTTGAGGGTGACGGCCTCATAGTTGCGACGCCTATGGGGAGCACCGCCTATAGCTACAGCGCGGGCGGCCCCGTCCTCTA
CCTCGACCTGGACGCGGTGGTGTTGACCCCGGTGAACCCCCTAGACCGGCGGTATGGGAGCGTGGTGGTTCCCGGGCGGC
CGGGGGTGGAGGTAGAGCTTATACACGCGACCAGGCCGGCCAAGCTGATAGTGGATGGTGTCTACGAGAAGCTGCTGTCC
CGCGGGGCCGTGGTGAGGGCTTGCCTGTGCGGGCCGCCGGTGAGGATAGCCCGGTACCGGGGGGTGAGGAGGCTGAGGAC
GCCCTGGAGCTAG

Upstream 100 bases:

>100_bases
GTTCTTAGCCCGTAGCCCATGCTTTTTCAGCTCGGCGCGTACGGTCTCGCCGCATCTCGGCTCCGTTACTAGCAGCGTGC
AGTTGCCTGGTGGGCCTGGG

Downstream 100 bases:

>100_bases
TCTACGTGCTCGACACGACCGCGTTCATAGCCAGGTGGCCCCTCTACGCGCCGAGGGGCAGGATGTACACTACGAGCCTC
GTCCTGGGCGAGGTCCGGGA

Product: ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLRRVAIYARPDRREALELAREAYRRLREAGAEAFYDASIAGLVGGPSTDVRFDDVDGVVVIGGDGTLLRLLQLLGSKTP
VLHLVRLGRKAFFFDEEPGEALDRIGDFVAGHFEVEQRVRLHVEVQGVPVYAFNEAAVLGSGSKILVVRVRAGDDTVYER
LEGDGLIVATPMGSTAYSYSAGGPVLYLDLDAVVLTPVNPLDRRYGSVVVPGRPGVEVELIHATRPAKLIVDGVYEKLLS
RGAVVRACLCGPPVRIARYRGVRRLRTPWS

Sequences:

>Translated_270_residues
MLRRVAIYARPDRREALELAREAYRRLREAGAEAFYDASIAGLVGGPSTDVRFDDVDGVVVIGGDGTLLRLLQLLGSKTP
VLHLVRLGRKAFFFDEEPGEALDRIGDFVAGHFEVEQRVRLHVEVQGVPVYAFNEAAVLGSGSKILVVRVRAGDDTVYER
LEGDGLIVATPMGSTAYSYSAGGPVLYLDLDAVVLTPVNPLDRRYGSVVVPGRPGVEVELIHATRPAKLIVDGVYEKLLS
RGAVVRACLCGPPVRIARYRGVRRLRTPWS
>Mature_270_residues
MLRRVAIYARPDRREALELAREAYRRLREAGAEAFYDASIAGLVGGPSTDVRFDDVDGVVVIGGDGTLLRLLQLLGSKTP
VLHLVRLGRKAFFFDEEPGEALDRIGDFVAGHFEVEQRVRLHVEVQGVPVYAFNEAAVLGSGSKILVVRVRAGDDTVYER
LEGDGLIVATPMGSTAYSYSAGGPVLYLDLDAVVLTPVNPLDRRYGSVVVPGRPGVEVELIHATRPAKLIVDGVYEKLLS
RGAVVRACLCGPPVRIARYRGVRRLRTPWS

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=217, Percent_Identity=29.9539170506912, Blast_Score=79, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322509, Length=226, Percent_Identity=27.8761061946903, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320794, Length=189, Percent_Identity=24.8677248677249, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325068, Length=214, Percent_Identity=26.6355140186916, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 29462; Mature: 29462

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRVAIYARPDRREALELAREAYRRLREAGAEAFYDASIAGLVGGPSTDVRFDDVDGVV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCEEECCCCEE
VIGGDGTLLRLLQLLGSKTPVLHLVRLGRKAFFFDEEPGEALDRIGDFVAGHFEVEQRVR
EECCCCHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCEEECCEEE
LHVEVQGVPVYAFNEAAVLGSGSKILVVRVRAGDDTVYERLEGDGLIVATPMGSTAYSYS
EEEEEECEEEEEECCEEEECCCCEEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCEEEC
AGGPVLYLDLDAVVLTPVNPLDRRYGSVVVPGRPGVEVELIHATRPAKLIVDGVYEKLLS
CCCCEEEEEECEEEECCCCHHHHCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHH
RGAVVRACLCGPPVRIARYRGVRRLRTPWS
CCHHEEHHHCCCCHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MLRRVAIYARPDRREALELAREAYRRLREAGAEAFYDASIAGLVGGPSTDVRFDDVDGVV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECCCCCCCCEEECCCCEE
VIGGDGTLLRLLQLLGSKTPVLHLVRLGRKAFFFDEEPGEALDRIGDFVAGHFEVEQRVR
EECCCCHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCEEECCEEE
LHVEVQGVPVYAFNEAAVLGSGSKILVVRVRAGDDTVYERLEGDGLIVATPMGSTAYSYS
EEEEEECEEEEEECCEEEECCCCEEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCEEEC
AGGPVLYLDLDAVVLTPVNPLDRRYGSVVVPGRPGVEVELIHATRPAKLIVDGVYEKLLS
CCCCEEEEEECEEEECCCCHHHHCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHH
RGAVVRACLCGPPVRIARYRGVRRLRTPWS
CCHHEEHHHCCCCHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]