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The map label for this gene is metG [H]

Identifier: 124025722

GI number: 124025722

Start: 941338

End: 942888

Strand: Direct

Name: metG [H]

Synonym: NATL1_10151

Alternate gene names: 124025722

Gene position: 941338-942888 (Clockwise)

Preceding gene: 124025721

Following gene: 124025723

Centisome position: 50.48

GC content: 32.3

Gene sequence:

>1551_bases
ATGAATATGAAATACACAATTACAACACCACTATATTATGTTAACGACAAGCCTCATCTAGGTAGTTCATATACAACAAT
CGCTTGTGATGCAATAGCTCGTTTTGAAAGACTAAATGGAAATACTGTCCTTTTTCTTACTGGTGTTGACGAACATGGTC
AGAAAATAGAACGTACTGCAGAAAGCAAAAACCTTGAACCTCAACTACATTGCGATGAAATCACGGAAAAGTATAAGTAT
TTATGGGATAAACTAAATATTAGTTACGATCGTTTCGTTAGAACAACTTCTATTGAACATACATCTTTGGTCCATCAATT
TTATTCCAAAGTTTTGAAATCTGATGATGTATACATGGGTAGGCAAAGTGGTTGGTATTGTGTTGGATGTGAGGAATATA
AGGATGTTGAGGATAAAGATAAAAGTCCAATCTGCTCAATACATAAAAAGGAATTAGAGTGGAGAGATGAAGAGAATTTA
TTTTTTAAATTATCAAAATATCAAGATCAAATTGAAAAATTAATTAATGTCGATGGTTTCATATCTCCAAAAAGTAGAAA
AAATGAAATTATAAATTTTGTCTCCAAGGGCTTAAGAGATTTCTCAATTTCAAGAGTAAACTTAAAATGGGGAATAAAAG
TTCCAGGAAATAAGGATCATACATTTTATGTATGGTTTGACGCTTTACTTGGTTATGTTAGTGGATCTCTTAATGATCAA
TGCTCACCGGAACTAACTGAAAAATCGTTATGTAATTGGCCTGCAAACAGACATGTTATTGGTAAGGATATCCTCAGATT
CCACGCTGTTTATTGGCCTGCAATGCTTCTGTCCGCAGGTATGAAAACACCACGAAGTGTTTTTGGTCATGGGTTCTTAA
CACGAGAAGGGCAGAAAATGGGTAAATCACTAGGTAACGTACTTGACCCTATTGAACTTCTGGAATATGGCGGGATCGAT
GCAATGAGATGGTATCTTCTAAGAGATATTGAATTTGGAGAAGATGGTGATTTTCAAATGAGAAGATTTGTTGATATCGT
TAATAGTGATTTATGTAATACTATTGGTAATTTATTAAATAGAACAATAACAATGTCGAAAAAATGGTTTAAAGAAAATA
TACCAATTGATAATACTGCATCATCTGACTCTACACTTAAAGTTCAATCAGAGACAAAGATAATCGAATATATGGAGTCA
TTTAAGGATAGTAATTTTAAAAGCTCAGCTAATGCAATAATTGATTTAGCCAATAGTGCAAATCTTTATCTAAATGATCG
AGCACCATGGAAACTAATTAAGGATATTTCAAATAAAAATGTTGTAGCCCTTGATATTTATTCTGTTCTCGAATCATGCC
GGATTATTGGAATATTACTTAATCCGATCGTACCAAATTTAAGTACTAGAATATTAAAACAACTTAATATTGACACTTCA
AATATAAATTTTAAAGATTCACTGCAATGGGGTTTATTAGATCCTAATAATAGATTACAGGACCCGACTCCTGTAATGGA
TAAGATTGAATTTAAAGAGGACCCTAAATAG

Upstream 100 bases:

>100_bases
AACGATATGAATTATTTTGAGATAATTCAATGATTAATATTAACTATAAACATAGAAGTGCAAAAGCCTTAAAAATATAA
TATAATAAACAGAATATGTA

Downstream 100 bases:

>100_bases
TGAACTCGTTCAATATCTATTTACTATTTATACTTACTATATTTCCACTTTTAGGATGTCAGAAATCTAATAAATCTACT
GCACAGATTGATAACAAAAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MNMKYTITTPLYYVNDKPHLGSSYTTIACDAIARFERLNGNTVLFLTGVDEHGQKIERTAESKNLEPQLHCDEITEKYKY
LWDKLNISYDRFVRTTSIEHTSLVHQFYSKVLKSDDVYMGRQSGWYCVGCEEYKDVEDKDKSPICSIHKKELEWRDEENL
FFKLSKYQDQIEKLINVDGFISPKSRKNEIINFVSKGLRDFSISRVNLKWGIKVPGNKDHTFYVWFDALLGYVSGSLNDQ
CSPELTEKSLCNWPANRHVIGKDILRFHAVYWPAMLLSAGMKTPRSVFGHGFLTREGQKMGKSLGNVLDPIELLEYGGID
AMRWYLLRDIEFGEDGDFQMRRFVDIVNSDLCNTIGNLLNRTITMSKKWFKENIPIDNTASSDSTLKVQSETKIIEYMES
FKDSNFKSSANAIIDLANSANLYLNDRAPWKLIKDISNKNVVALDIYSVLESCRIIGILLNPIVPNLSTRILKQLNIDTS
NINFKDSLQWGLLDPNNRLQDPTPVMDKIEFKEDPK

Sequences:

>Translated_516_residues
MNMKYTITTPLYYVNDKPHLGSSYTTIACDAIARFERLNGNTVLFLTGVDEHGQKIERTAESKNLEPQLHCDEITEKYKY
LWDKLNISYDRFVRTTSIEHTSLVHQFYSKVLKSDDVYMGRQSGWYCVGCEEYKDVEDKDKSPICSIHKKELEWRDEENL
FFKLSKYQDQIEKLINVDGFISPKSRKNEIINFVSKGLRDFSISRVNLKWGIKVPGNKDHTFYVWFDALLGYVSGSLNDQ
CSPELTEKSLCNWPANRHVIGKDILRFHAVYWPAMLLSAGMKTPRSVFGHGFLTREGQKMGKSLGNVLDPIELLEYGGID
AMRWYLLRDIEFGEDGDFQMRRFVDIVNSDLCNTIGNLLNRTITMSKKWFKENIPIDNTASSDSTLKVQSETKIIEYMES
FKDSNFKSSANAIIDLANSANLYLNDRAPWKLIKDISNKNVVALDIYSVLESCRIIGILLNPIVPNLSTRILKQLNIDTS
NINFKDSLQWGLLDPNNRLQDPTPVMDKIEFKEDPK
>Mature_516_residues
MNMKYTITTPLYYVNDKPHLGSSYTTIACDAIARFERLNGNTVLFLTGVDEHGQKIERTAESKNLEPQLHCDEITEKYKY
LWDKLNISYDRFVRTTSIEHTSLVHQFYSKVLKSDDVYMGRQSGWYCVGCEEYKDVEDKDKSPICSIHKKELEWRDEENL
FFKLSKYQDQIEKLINVDGFISPKSRKNEIINFVSKGLRDFSISRVNLKWGIKVPGNKDHTFYVWFDALLGYVSGSLNDQ
CSPELTEKSLCNWPANRHVIGKDILRFHAVYWPAMLLSAGMKTPRSVFGHGFLTREGQKMGKSLGNVLDPIELLEYGGID
AMRWYLLRDIEFGEDGDFQMRRFVDIVNSDLCNTIGNLLNRTITMSKKWFKENIPIDNTASSDSTLKVQSETKIIEYMES
FKDSNFKSSANAIIDLANSANLYLNDRAPWKLIKDISNKNVVALDIYSVLESCRIIGILLNPIVPNLSTRILKQLNIDTS
NINFKDSLQWGLLDPNNRLQDPTPVMDKIEFKEDPK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=510, Percent_Identity=31.9607843137255, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI14043022, Length=551, Percent_Identity=25.7713248638838, Blast_Score=152, Evalue=6e-37,
Organism=Escherichia coli, GI1788432, Length=513, Percent_Identity=26.3157894736842, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI212645096, Length=512, Percent_Identity=30.46875, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71992781, Length=407, Percent_Identity=28.992628992629, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17540794, Length=556, Percent_Identity=25.3597122302158, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321610, Length=518, Percent_Identity=32.4324324324324, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6321704, Length=541, Percent_Identity=25.1386321626617, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358483, Length=502, Percent_Identity=32.2709163346614, Blast_Score=277, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24655311, Length=550, Percent_Identity=26.5454545454545, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59458; Mature: 59458

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKYTITTPLYYVNDKPHLGSSYTTIACDAIARFERLNGNTVLFLTGVDEHGQKIERTA
CCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
ESKNLEPQLHCDEITEKYKYLWDKLNISYDRFVRTTSIEHTSLVHQFYSKVLKSDDVYMG
HHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCCEEEC
RQSGWYCVGCEEYKDVEDKDKSPICSIHKKELEWRDEENLFFKLSKYQDQIEKLINVDGF
CCCCEEEECCHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCCCC
ISPKSRKNEIINFVSKGLRDFSISRVNLKWGIKVPGNKDHTFYVWFDALLGYVSGSLNDQ
CCCCHHHHHHHHHHHHCCHHCCEEEEEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCCCC
CSPELTEKSLCNWPANRHVIGKDILRFHAVYWPAMLLSAGMKTPRSVFGHGFLTREGQKM
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHH
GKSLGNVLDPIELLEYGGIDAMRWYLLRDIEFGEDGDFQMRRFVDIVNSDLCNTIGNLLN
HHHHHHHHCHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RTITMSKKWFKENIPIDNTASSDSTLKVQSETKIIEYMESFKDSNFKSSANAIIDLANSA
HHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
NLYLNDRAPWKLIKDISNKNVVALDIYSVLESCRIIGILLNPIVPNLSTRILKQLNIDTS
EEEECCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
NINFKDSLQWGLLDPNNRLQDPTPVMDKIEFKEDPK
CCCCCCCCEECCCCCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MNMKYTITTPLYYVNDKPHLGSSYTTIACDAIARFERLNGNTVLFLTGVDEHGQKIERTA
CCCEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
ESKNLEPQLHCDEITEKYKYLWDKLNISYDRFVRTTSIEHTSLVHQFYSKVLKSDDVYMG
HHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHEECCCCHHHHHHHHHHHHHHCCCCEEEC
RQSGWYCVGCEEYKDVEDKDKSPICSIHKKELEWRDEENLFFKLSKYQDQIEKLINVDGF
CCCCEEEECCHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCCCC
ISPKSRKNEIINFVSKGLRDFSISRVNLKWGIKVPGNKDHTFYVWFDALLGYVSGSLNDQ
CCCCHHHHHHHHHHHHCCHHCCEEEEEEEEEEECCCCCCCEEEEEHHHHHHHHCCCCCCC
CSPELTEKSLCNWPANRHVIGKDILRFHAVYWPAMLLSAGMKTPRSVFGHGFLTREGQKM
CCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHH
GKSLGNVLDPIELLEYGGIDAMRWYLLRDIEFGEDGDFQMRRFVDIVNSDLCNTIGNLLN
HHHHHHHHCHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RTITMSKKWFKENIPIDNTASSDSTLKVQSETKIIEYMESFKDSNFKSSANAIIDLANSA
HHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCEEEECCCC
NLYLNDRAPWKLIKDISNKNVVALDIYSVLESCRIIGILLNPIVPNLSTRILKQLNIDTS
EEEECCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCC
NINFKDSLQWGLLDPNNRLQDPTPVMDKIEFKEDPK
CCCCCCCCEECCCCCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]