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The map label for this gene is iscS [H]

Identifier: 124025703

GI number: 124025703

Start: 917474

End: 918655

Strand: Direct

Name: iscS [H]

Synonym: NATL1_09961

Alternate gene names: 124025703

Gene position: 917474-918655 (Clockwise)

Preceding gene: 124025702

Following gene: 124025704

Centisome position: 49.2

GC content: 33.42

Gene sequence:

>1182_bases
ATGAAAGATATTTATCTCGATGCATCAGCGACTACTCCTCCTCATTTAGATGTTATTACCAAATTAAAGGATATTCAATT
TGAATGCTGGGGTAATCCATCAAGTATTCATAAAGTTGGGGTTATCGCAAGAGAGATTTTAGAAAGATCAAGATTATCAA
TAGCAAATAAATTAAAAGCGTCTTCAGACGAATTATTTTTTACATCTGGAGCAACTGAATCAAATTTTCTAGCTCTAAAA
GCTGTATCTAATAATCTTGATAAAGGAAGAATTGTCATTTCTAGCGTTGAACACCCCTCGATTAACCTAATAGCTAATCA
ATTGCGTAATGAAGGCTGGGATATTAAATACTGGCCTGTGGATAGTTATGGAATTATTGATCTAGATTTATTAGAGGAGG
TGTTATCTCCTCCGACAAAATTGGTTTCAATAATCTGGGGACAAAGTGAGATTGGCTCCATTCAGCCCATAAATCTCATA
GGTATGGAATGTAAGAAACGAAATATTCTTTTCCATACAGATGCCACACAGGTCCTCCCTTGTGGCCTTATTGATTGGAG
TAATTTAAATGTCGATATGCTTAGCGCCTCTGCTCATAAGCTTCAAGGCCCTAAAGGTATTGGATTATTGATGTTGCGAA
AGGGTATTCAAGATTTATTAATGAAGAATCCTTCATATGGATTTAAAAATGGCTCAATAAGATCTGGTACTGAATCAGTT
CCTCTTATTGCAGGTTTTTCGACGGCAATTGACTTACTCAATGAATATATAGAGGTTAAAGATAATCAAACTTTATTTCC
TGTGAATAATGTTTCAAAAATGACTTCTCGTTTAAAGAAAAATCTAATTAATAATAAACATTTTACGTTTATCGGACCAG
ATATAGAAAGACTACCCAATAATCTTTCATTTCTCTGTCATACAGAATCAATGATCCCGATTAAAGGTAGAGAAATTGTT
CGATTATTGTCAAAACATGGAGTTTATATAAGTAGCGGTAGTGCCTGCTCTTCATCTATTCAAGGACCAAATCCAATACT
GGTAGCAATTAATGTTGACAAAACATTTCAAGAATCTGGATTAAGAATTACTATTGGTCCATGGATTTCAAATGATGATA
TTACCTCAGTTTCAAATATAATATTCGAATCATTGCAAACTTTAGAACTCAAAAAACAATGA

Upstream 100 bases:

>100_bases
AATTAGAAGTTGAATGGCCAAATCAGTCAGGTCCCGTTTTCATGCAAGGACCAGCTTTAAAAGTATTTAGTGGAGAGATA
GATATTTAATAAGTCCTCAT

Downstream 100 bases:

>100_bases
TTATCAAATAATGAATATGTTGCCTTCAGATCTTAAAGAAGCAGAATCAAATGTATATGAATCTGTTCAAAGTTACTTTT
TAAGCAACTCCGAACAATCA

Product: class-V aminotransferase family cysteine desulfurase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKDIYLDASATTPPHLDVITKLKDIQFECWGNPSSIHKVGVIAREILERSRLSIANKLKASSDELFFTSGATESNFLALK
AVSNNLDKGRIVISSVEHPSINLIANQLRNEGWDIKYWPVDSYGIIDLDLLEEVLSPPTKLVSIIWGQSEIGSIQPINLI
GMECKKRNILFHTDATQVLPCGLIDWSNLNVDMLSASAHKLQGPKGIGLLMLRKGIQDLLMKNPSYGFKNGSIRSGTESV
PLIAGFSTAIDLLNEYIEVKDNQTLFPVNNVSKMTSRLKKNLINNKHFTFIGPDIERLPNNLSFLCHTESMIPIKGREIV
RLLSKHGVYISSGSACSSSIQGPNPILVAINVDKTFQESGLRITIGPWISNDDITSVSNIIFESLQTLELKKQ

Sequences:

>Translated_393_residues
MKDIYLDASATTPPHLDVITKLKDIQFECWGNPSSIHKVGVIAREILERSRLSIANKLKASSDELFFTSGATESNFLALK
AVSNNLDKGRIVISSVEHPSINLIANQLRNEGWDIKYWPVDSYGIIDLDLLEEVLSPPTKLVSIIWGQSEIGSIQPINLI
GMECKKRNILFHTDATQVLPCGLIDWSNLNVDMLSASAHKLQGPKGIGLLMLRKGIQDLLMKNPSYGFKNGSIRSGTESV
PLIAGFSTAIDLLNEYIEVKDNQTLFPVNNVSKMTSRLKKNLINNKHFTFIGPDIERLPNNLSFLCHTESMIPIKGREIV
RLLSKHGVYISSGSACSSSIQGPNPILVAINVDKTFQESGLRITIGPWISNDDITSVSNIIFESLQTLELKKQ
>Mature_393_residues
MKDIYLDASATTPPHLDVITKLKDIQFECWGNPSSIHKVGVIAREILERSRLSIANKLKASSDELFFTSGATESNFLALK
AVSNNLDKGRIVISSVEHPSINLIANQLRNEGWDIKYWPVDSYGIIDLDLLEEVLSPPTKLVSIIWGQSEIGSIQPINLI
GMECKKRNILFHTDATQVLPCGLIDWSNLNVDMLSASAHKLQGPKGIGLLMLRKGIQDLLMKNPSYGFKNGSIRSGTESV
PLIAGFSTAIDLLNEYIEVKDNQTLFPVNNVSKMTSRLKKNLINNKHFTFIGPDIERLPNNLSFLCHTESMIPIKGREIV
RLLSKHGVYISSGSACSSSIQGPNPILVAINVDKTFQESGLRITIGPWISNDDITSVSNIIFESLQTLELKKQ

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=377, Percent_Identity=31.5649867374005, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI156713448, Length=427, Percent_Identity=28.8056206088993, Blast_Score=148, Evalue=9e-36,
Organism=Escherichia coli, GI48994898, Length=396, Percent_Identity=33.5858585858586, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789175, Length=412, Percent_Identity=22.0873786407767, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787970, Length=220, Percent_Identity=26.3636363636364, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25143064, Length=379, Percent_Identity=32.9815303430079, Blast_Score=187, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17533177, Length=343, Percent_Identity=27.6967930029155, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319831, Length=391, Percent_Identity=33.5038363171356, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI20129463, Length=392, Percent_Identity=31.6326530612245, Blast_Score=187, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 43493; Mature: 43493

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIYLDASATTPPHLDVITKLKDIQFECWGNPSSIHKVGVIAREILERSRLSIANKLKA
CCEEEEECCCCCCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSDELFFTSGATESNFLALKAVSNNLDKGRIVISSVEHPSINLIANQLRNEGWDIKYWPV
CCCCEEEECCCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEC
DSYGIIDLDLLEEVLSPPTKLVSIIWGQSEIGSIQPINLIGMECKKRNILFHTDATQVLP
CCCCEEEHHHHHHHHCCHHHHHHEEECCCCCCCCCCEEEECCCCCCCCEEEECCCCHHCC
CGLIDWSNLNVDMLSASAHKLQGPKGIGLLMLRKGIQDLLMKNPSYGFKNGSIRSGTESV
CCEEECCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PLIAGFSTAIDLLNEYIEVKDNQTLFPVNNVSKMTSRLKKNLINNKHFTFIGPDIERLPN
CEEECHHHHHHHHHHHHEECCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECCCHHHCCC
NLSFLCHTESMIPIKGREIVRLLSKHGVYISSGSACSSSIQGPNPILVAINVDKTFQESG
CCEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHCC
LRITIGPWISNDDITSVSNIIFESLQTLELKKQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDIYLDASATTPPHLDVITKLKDIQFECWGNPSSIHKVGVIAREILERSRLSIANKLKA
CCEEEEECCCCCCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
SSDELFFTSGATESNFLALKAVSNNLDKGRIVISSVEHPSINLIANQLRNEGWDIKYWPV
CCCCEEEECCCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEC
DSYGIIDLDLLEEVLSPPTKLVSIIWGQSEIGSIQPINLIGMECKKRNILFHTDATQVLP
CCCCEEEHHHHHHHHCCHHHHHHEEECCCCCCCCCCEEEECCCCCCCCEEEECCCCHHCC
CGLIDWSNLNVDMLSASAHKLQGPKGIGLLMLRKGIQDLLMKNPSYGFKNGSIRSGTESV
CCEEECCCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
PLIAGFSTAIDLLNEYIEVKDNQTLFPVNNVSKMTSRLKKNLINNKHFTFIGPDIERLPN
CEEECHHHHHHHHHHHHEECCCCEEECCCHHHHHHHHHHHHHHCCCCEEEECCCHHHCCC
NLSFLCHTESMIPIKGREIVRLLSKHGVYISSGSACSSSIQGPNPILVAINVDKTFQESG
CCEEEEECCCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEEEECCCHHHHCC
LRITIGPWISNDDITSVSNIIFESLQTLELKKQ
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]