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The map label for this gene is dapF

Identifier: 124025702

GI number: 124025702

Start: 916608

End: 917462

Strand: Direct

Name: dapF

Synonym: NATL1_09951

Alternate gene names: 124025702

Gene position: 916608-917462 (Clockwise)

Preceding gene: 124025700

Following gene: 124025703

Centisome position: 49.15

GC content: 32.28

Gene sequence:

>855_bases
ATGAAAAATAATTTTTCAAAATATCAAGGACTTGGTAATGATTTTATTATCTTTGATGCTCGTAGTAACAATTTAGATCA
CTTATTTTCAAAAAATAAAGATAATTTTATTGAGCACCTATGTAATAGAAATTTTGGCATCGGAGCTGATGGGATTATTC
TTATATTAGAGTCTAATAATAAATGTTTTGTAAGAATGAAAATTTATAATTCTGATGGTTCCGAGCCAGAAATGTGTGGC
AATGGTATTAGATGTCTGATTGCTTTTTTGAATGATAATAATGAAATCAATGAATTGTCTGAGATCCCTATTGAGACCAA
AGCGGGTCTTATCCTTACTTCTATTGATTGTAATGAAAATATAAAAGTAAATATGGGTGAACCTATCCTTTCTCCTCTAG
ATATACCCACGAAATTATTGATGAACAGTTTAAAAGTTCCTAATGGTGTGATTACATTAAAAGATCAAATATTAAATGTT
TATGCTGCTAGTATGGGGAATCCTCATATGATCGTTTTTGTTAATGATATTGAAGGGATACCATTTCAAGAATGGGGTAG
TTTCCTTGAAAAACATAATACATTTCCAAATGATACCAATGTTCACTTTGTTGAAATAATCGATAAGTCAAATATTAAAG
TAAAGGTATGGGAAAGGGGTTGCGGCCCAACATTAGCTTGTGGTACTGGAGCATGCGCTTGTCTGGTAGTTACCTCTAAG
TTAGGGAAAACTTTAAACAACGCTAATGTCTATTTACCAGGCGGCAAATTAGAAGTTGAATGGCCAAATCAGTCAGGTCC
CGTTTTCATGCAAGGACCAGCTTTAAAAGTATTTAGTGGAGAGATAGATATTTAA

Upstream 100 bases:

>100_bases
ATAATGTTCTCATAAATTGATGCGCAATATGCCAATAGTTCTGAAATTACTACCAAGAGCCCTTTATTTCCTACAAACAT
AGTTTAACAATCAGAAAATC

Downstream 100 bases:

>100_bases
TAAGTCCTCATATGAAAGATATTTATCTCGATGCATCAGCGACTACTCCTCCTCATTTAGATGTTATTACCAAATTAAAG
GATATTCAATTTGAATGCTG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKNNFSKYQGLGNDFIIFDARSNNLDHLFSKNKDNFIEHLCNRNFGIGADGIILILESNNKCFVRMKIYNSDGSEPEMCG
NGIRCLIAFLNDNNEINELSEIPIETKAGLILTSIDCNENIKVNMGEPILSPLDIPTKLLMNSLKVPNGVITLKDQILNV
YAASMGNPHMIVFVNDIEGIPFQEWGSFLEKHNTFPNDTNVHFVEIIDKSNIKVKVWERGCGPTLACGTGACACLVVTSK
LGKTLNNANVYLPGGKLEVEWPNQSGPVFMQGPALKVFSGEIDI

Sequences:

>Translated_284_residues
MKNNFSKYQGLGNDFIIFDARSNNLDHLFSKNKDNFIEHLCNRNFGIGADGIILILESNNKCFVRMKIYNSDGSEPEMCG
NGIRCLIAFLNDNNEINELSEIPIETKAGLILTSIDCNENIKVNMGEPILSPLDIPTKLLMNSLKVPNGVITLKDQILNV
YAASMGNPHMIVFVNDIEGIPFQEWGSFLEKHNTFPNDTNVHFVEIIDKSNIKVKVWERGCGPTLACGTGACACLVVTSK
LGKTLNNANVYLPGGKLEVEWPNQSGPVFMQGPALKVFSGEIDI
>Mature_284_residues
MKNNFSKYQGLGNDFIIFDARSNNLDHLFSKNKDNFIEHLCNRNFGIGADGIILILESNNKCFVRMKIYNSDGSEPEMCG
NGIRCLIAFLNDNNEINELSEIPIETKAGLILTSIDCNENIKVNMGEPILSPLDIPTKLLMNSLKVPNGVITLKDQILNV
YAASMGNPHMIVFVNDIEGIPFQEWGSFLEKHNTFPNDTNVHFVEIIDKSNIKVKVWERGCGPTLACGTGACACLVVTSK
LGKTLNNANVYLPGGKLEVEWPNQSGPVFMQGPALKVFSGEIDI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=282, Percent_Identity=33.3333333333333, Blast_Score=183, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_PROM1 (A2C243)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014818.1
- ProteinModelPortal:   A2C243
- SMR:   A2C243
- STRING:   A2C243
- GeneID:   4779633
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_09951
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   PMAR167555:NATL1_09951-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31369; Mature: 31369

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 79-79 ACT_SITE 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNFSKYQGLGNDFIIFDARSNNLDHLFSKNKDNFIEHLCNRNFGIGADGIILILESNN
CCCCCCHHHCCCCCEEEEECCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEECCC
KCFVRMKIYNSDGSEPEMCGNGIRCLIAFLNDNNEINELSEIPIETKAGLILTSIDCNEN
EEEEEEEEECCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCC
IKVNMGEPILSPLDIPTKLLMNSLKVPNGVITLKDQILNVYAASMGNPHMIVFVNDIEGI
EEEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCEEEEEEECCCCC
PFQEWGSFLEKHNTFPNDTNVHFVEIIDKSNIKVKVWERGCGPTLACGTGACACLVVTSK
CHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEECCCCCEEEEEEEHH
LGKTLNNANVYLPGGKLEVEWPNQSGPVFMQGPALKVFSGEIDI
HCCCCCCCEEEECCCEEEEECCCCCCCEEEECCEEEEECCCCCC
>Mature Secondary Structure
MKNNFSKYQGLGNDFIIFDARSNNLDHLFSKNKDNFIEHLCNRNFGIGADGIILILESNN
CCCCCCHHHCCCCCEEEEECCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEECCC
KCFVRMKIYNSDGSEPEMCGNGIRCLIAFLNDNNEINELSEIPIETKAGLILTSIDCNEN
EEEEEEEEECCCCCCHHHHCCCEEEEEEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCC
IKVNMGEPILSPLDIPTKLLMNSLKVPNGVITLKDQILNVYAASMGNPHMIVFVNDIEGI
EEEECCCCCCCCCCCCHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCEEEEEEECCCCC
PFQEWGSFLEKHNTFPNDTNVHFVEIIDKSNIKVKVWERGCGPTLACGTGACACLVVTSK
CHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEECCCCCEEEEEEEHH
LGKTLNNANVYLPGGKLEVEWPNQSGPVFMQGPALKVFSGEIDI
HCCCCCCCEEEECCCEEEEECCCCCCCEEEECCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA