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The map label for this gene is ispE

Identifier: 124025655

GI number: 124025655

Start: 874226

End: 875185

Strand: Direct

Name: ispE

Synonym: NATL1_09481

Alternate gene names: 124025655

Gene position: 874226-875185 (Clockwise)

Preceding gene: 124025654

Following gene: 124025656

Centisome position: 46.88

GC content: 32.6

Gene sequence:

>960_bases
ATGGAACCTTCCAAAGCAAATGAAGATTTTCTTATCGCAAAAGCACATGCAAAAATTAATCTACATTTAGAGGTTTTAGG
TATTAGGAGCGATGGCTTTCATGAATTAGCAATGGTCATGCAAAGTATTAATTTAAGTGATCAGTTGAAGATGATAAAAA
GAGTAGATAATACTATTAATCTAAAATCTAATAATAAAGAAATTAGTAATGGTGACGATAATCTAATAATAAAAGCTTCA
AAGCTGTTGAGAAATAAAGTAGAAAATCAAGAATTAGGTGTTGATATTGAACTTGAGAAAAACATTCCTATTGGAGCAGG
ATTGGCAGGGGGATCTACAGATGCAGCTGCAACCTTACTTGGATTAAATAAACTCTGGAAGCTAAATCTTAAGACTGATG
AATTAGAGAACCTATCAAAAGAAATAGGATCAGATATCCCTTTTTGCATATCAGGAGGGAGGCAAATATGTTTTGGTAGA
GGTGAAATTTTAGAAAAATTGAAATTTGATCAAATTCAGTTAGGTCTTATTTTGGTTAAAGACCCTTCAATACAAGTATC
TACTCCAGTTGCATACAAAAAATATAAAGATCAGTTTGGTGAAAGCTATCTTGAAGATGATAGGGATTTTGAAATCAAAA
GAAACTCTATTAGATCTATTGACTGGTCTGATCAGTCGCTTTTTGATAATCGTAAAGAAATACAAAATGATTTACAAAAA
AGCGTTCGGCCTATAACACCAGAGGTTGAGAAGTCATTGGATTTATTGTCTAGTTTGCCAGATTCACGTCTTGTTTCAAT
GAGTGGTTCTGGTCCAAGTTGTTTTGCCTTGTTTCAAAATTATGACCAAGCAAATAAAGTACTCAAAGAACATGTTAATG
AATTTGAAAGGGCTGGTTTATCAGCTTGGGCATGTTCAATGATGTCTAATGGAGTTGAATTAAGAAATGAATTCATCTAG

Upstream 100 bases:

>100_bases
GCCTACAGAGGTATTAGTCTTGATCAAAGACCACAGGAAATTTCACCTTCCAATTGGTTCGGCTTGGCAAAAGCTTTAAA
AGAAACTTGTGTTATTGAAA

Downstream 100 bases:

>100_bases
TAATGGGTTTAAAAATGAATCTGATGAATTAGCTAATCAATCATCAAGTTCTTTAGATCCCAATAGCCCTGAACCTCAGA
AAGGCCCCATAAGCTTTCTC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MEPSKANEDFLIAKAHAKINLHLEVLGIRSDGFHELAMVMQSINLSDQLKMIKRVDNTINLKSNNKEISNGDDNLIIKAS
KLLRNKVENQELGVDIELEKNIPIGAGLAGGSTDAAATLLGLNKLWKLNLKTDELENLSKEIGSDIPFCISGGRQICFGR
GEILEKLKFDQIQLGLILVKDPSIQVSTPVAYKKYKDQFGESYLEDDRDFEIKRNSIRSIDWSDQSLFDNRKEIQNDLQK
SVRPITPEVEKSLDLLSSLPDSRLVSMSGSGPSCFALFQNYDQANKVLKEHVNEFERAGLSAWACSMMSNGVELRNEFI

Sequences:

>Translated_319_residues
MEPSKANEDFLIAKAHAKINLHLEVLGIRSDGFHELAMVMQSINLSDQLKMIKRVDNTINLKSNNKEISNGDDNLIIKAS
KLLRNKVENQELGVDIELEKNIPIGAGLAGGSTDAAATLLGLNKLWKLNLKTDELENLSKEIGSDIPFCISGGRQICFGR
GEILEKLKFDQIQLGLILVKDPSIQVSTPVAYKKYKDQFGESYLEDDRDFEIKRNSIRSIDWSDQSLFDNRKEIQNDLQK
SVRPITPEVEKSLDLLSSLPDSRLVSMSGSGPSCFALFQNYDQANKVLKEHVNEFERAGLSAWACSMMSNGVELRNEFI
>Mature_319_residues
MEPSKANEDFLIAKAHAKINLHLEVLGIRSDGFHELAMVMQSINLSDQLKMIKRVDNTINLKSNNKEISNGDDNLIIKAS
KLLRNKVENQELGVDIELEKNIPIGAGLAGGSTDAAATLLGLNKLWKLNLKTDELENLSKEIGSDIPFCISGGRQICFGR
GEILEKLKFDQIQLGLILVKDPSIQVSTPVAYKKYKDQFGESYLEDDRDFEIKRNSIRSIDWSDQSLFDNRKEIQNDLQK
SVRPITPEVEKSLDLLSSLPDSRLVSMSGSGPSCFALFQNYDQANKVLKEHVNEFERAGLSAWACSMMSNGVELRNEFI

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=291, Percent_Identity=31.2714776632302, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PROM1 (A2C1Z6)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014771.1
- ProteinModelPortal:   A2C1Z6
- SMR:   A2C1Z6
- STRING:   A2C1Z6
- GeneID:   4781268
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_09481
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- ProtClustDB:   PRK02534
- BioCyc:   PMAR167555:NATL1_09481-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 35717; Mature: 35717

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: ACT_SITE 18-18 ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPSKANEDFLIAKAHAKINLHLEVLGIRSDGFHELAMVMQSINLSDQLKMIKRVDNTIN
CCCCCCCCCEEEEEECEEEEEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCEEE
LKSNNKEISNGDDNLIIKASKLLRNKVENQELGVDIELEKNIPIGAGLAGGSTDAAATLL
ECCCCCCCCCCCCCEEEEHHHHHHHHCCCHHCCEEEEECCCCCCCCCCCCCCHHHHHHHH
GLNKLWKLNLKTDELENLSKEIGSDIPFCISGGRQICFGRGEILEKLKFDQIQLGLILVK
HHHHHEEECCCHHHHHHHHHHHCCCCCEEECCCCEEEECCHHHHHHHCCCEEEEEEEEEE
DPSIQVSTPVAYKKYKDQFGESYLEDDRDFEIKRNSIRSIDWSDQSLFDNRKEIQNDLQK
CCCEEECCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
SVRPITPEVEKSLDLLSSLPDSRLVSMSGSGPSCFALFQNYDQANKVLKEHVNEFERAGL
HCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SAWACSMMSNGVELRNEFI
HHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MEPSKANEDFLIAKAHAKINLHLEVLGIRSDGFHELAMVMQSINLSDQLKMIKRVDNTIN
CCCCCCCCCEEEEEECEEEEEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCEEE
LKSNNKEISNGDDNLIIKASKLLRNKVENQELGVDIELEKNIPIGAGLAGGSTDAAATLL
ECCCCCCCCCCCCCEEEEHHHHHHHHCCCHHCCEEEEECCCCCCCCCCCCCCHHHHHHHH
GLNKLWKLNLKTDELENLSKEIGSDIPFCISGGRQICFGRGEILEKLKFDQIQLGLILVK
HHHHHEEECCCHHHHHHHHHHHCCCCCEEECCCCEEEECCHHHHHHHCCCEEEEEEEEEE
DPSIQVSTPVAYKKYKDQFGESYLEDDRDFEIKRNSIRSIDWSDQSLFDNRKEIQNDLQK
CCCEEECCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
SVRPITPEVEKSLDLLSSLPDSRLVSMSGSGPSCFALFQNYDQANKVLKEHVNEFERAGL
HCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SAWACSMMSNGVELRNEFI
HHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA