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The map label for this gene is ksgA

Identifier: 124025654

GI number: 124025654

Start: 873373

End: 874248

Strand: Direct

Name: ksgA

Synonym: NATL1_09471

Alternate gene names: 124025654

Gene position: 873373-874248 (Clockwise)

Preceding gene: 124025648

Following gene: 124025655

Centisome position: 46.84

GC content: 36.07

Gene sequence:

>876_bases
ATGGGTGGTGATAACACTTTGAATGATTTTAGGCACATCCCAAGAAAACGCTTTGGACAACATTGGTTGAAAGATCAGGG
TGTTTTGGATCAAATAGTCAAGGCTGCCGAATTGAATCCTGAAGATTGTGTATTAGAAGTTGGTCCAGGTAAAGGTGCTT
TAACAGAAAAATTAATTGAATCCCAAGCAAAATTTATCCAGGCAATTGAGCTAGATAGAGATTTAGTCATTGGTTTGAAA
AAGCGTTTTAATCATCAAAATAAATTTAGTTTGAGAGAGGGAGACATCCTTTCTGCCCCACTGGATGCTGAGAATGGAGT
CACTATCAACAAAGTTGTAGCTAATATTCCATACAACATCACCGGTCCACTATTAAAAAGATTGATTGGTGAATTAAGAA
AAGCGCCTGAAAATAGTTTTGAAACTTTAGTTTTACTTATGCAAAAAGAAGTTGCACAAAGACTTTTAGCTAGACCTGGG
AATAGTAATTTTAGTGCTTTAAGTGTACGGGTCCAGCTTTTAGCTAAATGTCAGGACGTATGTGATGTACCTTCTAAATG
TTTTCAACCAGCACCGAAGGTGGATTCTAAAGTAGTCATGATTAAGCCCTTTGCATCTATTGATCCAGATTTTTATGAGG
TAGGGAATTTATTAGAGAAACTTTTGAAACATGCTTTTGCTGGTAGAAGAAAAAAATTACGAAACACGATTGGAAGTTTT
GTTACTTCCAACGATCAAATAAAGGAATTTTTCGCCTACAGAGGTATTAGTCTTGATCAAAGACCACAGGAAATTTCACC
TTCCAATTGGTTCGGCTTGGCAAAAGCTTTAAAAGAAACTTGTGTTATTGAAAATGGAACCTTCCAAAGCAAATGA

Upstream 100 bases:

>100_bases
TATATTGATTGAAGATCTTCTTGTGAGTATATTTTAAATAATTTATTTTTATTTGAGTGAAATATGTGATCGTTTCTAAT
GTGGTTTGGAGTTTATGCGT

Downstream 100 bases:

>100_bases
AGATTTTCTTATCGCAAAAGCACATGCAAAAATTAATCTACATTTAGAGGTTTTAGGTATTAGGAGCGATGGCTTTCATG
AATTAGCAATGGTCATGCAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MGGDNTLNDFRHIPRKRFGQHWLKDQGVLDQIVKAAELNPEDCVLEVGPGKGALTEKLIESQAKFIQAIELDRDLVIGLK
KRFNHQNKFSLREGDILSAPLDAENGVTINKVVANIPYNITGPLLKRLIGELRKAPENSFETLVLLMQKEVAQRLLARPG
NSNFSALSVRVQLLAKCQDVCDVPSKCFQPAPKVDSKVVMIKPFASIDPDFYEVGNLLEKLLKHAFAGRRKKLRNTIGSF
VTSNDQIKEFFAYRGISLDQRPQEISPSNWFGLAKALKETCVIENGTFQSK

Sequences:

>Translated_291_residues
MGGDNTLNDFRHIPRKRFGQHWLKDQGVLDQIVKAAELNPEDCVLEVGPGKGALTEKLIESQAKFIQAIELDRDLVIGLK
KRFNHQNKFSLREGDILSAPLDAENGVTINKVVANIPYNITGPLLKRLIGELRKAPENSFETLVLLMQKEVAQRLLARPG
NSNFSALSVRVQLLAKCQDVCDVPSKCFQPAPKVDSKVVMIKPFASIDPDFYEVGNLLEKLLKHAFAGRRKKLRNTIGSF
VTSNDQIKEFFAYRGISLDQRPQEISPSNWFGLAKALKETCVIENGTFQSK
>Mature_290_residues
GGDNTLNDFRHIPRKRFGQHWLKDQGVLDQIVKAAELNPEDCVLEVGPGKGALTEKLIESQAKFIQAIELDRDLVIGLKK
RFNHQNKFSLREGDILSAPLDAENGVTINKVVANIPYNITGPLLKRLIGELRKAPENSFETLVLLMQKEVAQRLLARPGN
SNFSALSVRVQLLAKCQDVCDVPSKCFQPAPKVDSKVVMIKPFASIDPDFYEVGNLLEKLLKHAFAGRRKKLRNTIGSFV
TSNDQIKEFFAYRGISLDQRPQEISPSNWFGLAKALKETCVIENGTFQSK

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=188, Percent_Identity=38.8297872340425, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI156415992, Length=283, Percent_Identity=25.4416961130742, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1786236, Length=277, Percent_Identity=33.2129963898917, Blast_Score=132, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25146882, Length=219, Percent_Identity=40.6392694063927, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25141369, Length=248, Percent_Identity=27.4193548387097, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=188, Percent_Identity=37.7659574468085, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21358017, Length=191, Percent_Identity=37.17277486911, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357273, Length=286, Percent_Identity=27.972027972028, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PROM1 (A2C1Z5)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014770.1
- ProteinModelPortal:   A2C1Z5
- SMR:   A2C1Z5
- STRING:   A2C1Z5
- GeneID:   4780584
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_09471
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   YYIATRL
- ProtClustDB:   PRK00274
- BioCyc:   PMAR167555:NATL1_09471-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32571; Mature: 32440

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 21-21 BINDING 23-23 BINDING 48-48 BINDING 70-70 BINDING 95-95 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGDNTLNDFRHIPRKRFGQHWLKDQGVLDQIVKAAELNPEDCVLEVGPGKGALTEKLIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEEEECCCCCHHHHHHHH
SQAKFIQAIELDRDLVIGLKKRFNHQNKFSLREGDILSAPLDAENGVTINKVVANIPYNI
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCC
TGPLLKRLIGELRKAPENSFETLVLLMQKEVAQRLLARPGNSNFSALSVRVQLLAKCQDV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
CDVPSKCFQPAPKVDSKVVMIKPFASIDPDFYEVGNLLEKLLKHAFAGRRKKLRNTIGSF
HCCCHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTSNDQIKEFFAYRGISLDQRPQEISPSNWFGLAKALKETCVIENGTFQSK
HCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GGDNTLNDFRHIPRKRFGQHWLKDQGVLDQIVKAAELNPEDCVLEVGPGKGALTEKLIE
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHEEEEECCCCCHHHHHHHH
SQAKFIQAIELDRDLVIGLKKRFNHQNKFSLREGDILSAPLDAENGVTINKVVANIPYNI
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHCCCCC
TGPLLKRLIGELRKAPENSFETLVLLMQKEVAQRLLARPGNSNFSALSVRVQLLAKCQDV
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
CDVPSKCFQPAPKVDSKVVMIKPFASIDPDFYEVGNLLEKLLKHAFAGRRKKLRNTIGSF
HCCCHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTSNDQIKEFFAYRGISLDQRPQEISPSNWFGLAKALKETCVIENGTFQSK
HCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA