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The map label for this gene is carA [H]

Identifier: 124025635

GI number: 124025635

Start: 856059

End: 857213

Strand: Direct

Name: carA [H]

Synonym: NATL1_09281

Alternate gene names: 124025635

Gene position: 856059-857213 (Clockwise)

Preceding gene: 124025634

Following gene: 124025636

Centisome position: 45.91

GC content: 34.72

Gene sequence:

>1155_bases
ATGTTTTTTGATACTGATAGCTCTGCAATTTTGTTATTAGAGGATGGGACATATTTTGAAGGATTATCTTTTGGTGCGGT
TGGAACTATTTCTGGTGAAGTCGTATTTAATACAGGTATGACAGGTTATCAGGAGGTCATTACTGATCCAAGTTATTTTG
GTCAACTTATTACGTTTACCTATCCAGAGATTGGAAATACTGGAGTCAATTATGAGGATAATGAATCATCACATCCTTCA
GTTAAAGGTGTGATAGCTCGTCAAATATCCAAAACTCCCAGTAACTGGAGACATGAAATTTCGTTCGAAAATTGGTTAAA
GGATGAAAATGTTGTTGGAATTCATGGAATAGATACAAGATTACTTGTTAGACATATAAGAGAGTCAGGTTCTTTGAATG
GAATTATTTCATCAGAGTCAAAATATTCAATCGCTGAATTATTTTCACTTTTAAAAAAATCTCCTTCAATGAGTGGTCTT
AATCTTGTCGATAAAGTAACAACAAAAAGTGCTTTTAATGCTAATTCAAGTTGTCCTGTTGCATTTGATATGAGAATTAA
AAATACTCAAACAATTCCATACAAAGTTGTTGCTATAGATTTTGGGATAAAGCAATCGATATTGGATCGATTAGTTGCTC
ATGGTTGTGAAGTAACTGTCTTGCCTGCAAGTGCTGAAATGACCGATGTATTAGCTTTATCCCCTGAAGGTGTTTTTCTT
TCCAATGGTCCTGGTGATCCTTCCACCGTCGATAGTGGTATTAATCTTGCTAAAGATTTAATAGAATATAAAAAACTACC
TGTTTTTGGAATCTGCTTAGGCCATCAAATCCTTGGATTGGCTTTAGGTGGTAAAACTTTTAAACTTTCTTATGGACATA
GAGGACTAAATCATCCATGTGGATTGAACGGAAAAGTTGAAATTACTAGTCAAAACCATGGATTTGCTTTGAATTCAGAA
TCTTTAAATTCCGAGAAAATAAAAATCACCAGATTAAATTTAAACGACCAAACAGTCGCTGCTATTTCTGTTATTGATAG
GCCTTTCTTTGGTGTTCAGTATCACCCCGAAGCGAGCCCTGGTCCTCATGATGCTGATCATCATTTTAATCATTTCGTAA
CCTTAATAGAAGAACGACGAAGAATCGTGGATTAA

Upstream 100 bases:

>100_bases
CATTAGATTGTTTGAATACAGATAAATCATGGCTCCTTTTCGAGCAATTAAGAGATTTTTTAGCCACTTAATTCTTTCAA
TCTGATAAACCCTATTCCTT

Downstream 100 bases:

>100_bases
TTTGGTTTCTATCACTACACTTCCATCATGAAGTCATAGGGGACTAATCCCATAACTGAATTACAAAGACTTACTGTTTC
TCTTAGAGGTGGTTCTAACC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MFFDTDSSAILLLEDGTYFEGLSFGAVGTISGEVVFNTGMTGYQEVITDPSYFGQLITFTYPEIGNTGVNYEDNESSHPS
VKGVIARQISKTPSNWRHEISFENWLKDENVVGIHGIDTRLLVRHIRESGSLNGIISSESKYSIAELFSLLKKSPSMSGL
NLVDKVTTKSAFNANSSCPVAFDMRIKNTQTIPYKVVAIDFGIKQSILDRLVAHGCEVTVLPASAEMTDVLALSPEGVFL
SNGPGDPSTVDSGINLAKDLIEYKKLPVFGICLGHQILGLALGGKTFKLSYGHRGLNHPCGLNGKVEITSQNHGFALNSE
SLNSEKIKITRLNLNDQTVAAISVIDRPFFGVQYHPEASPGPHDADHHFNHFVTLIEERRRIVD

Sequences:

>Translated_384_residues
MFFDTDSSAILLLEDGTYFEGLSFGAVGTISGEVVFNTGMTGYQEVITDPSYFGQLITFTYPEIGNTGVNYEDNESSHPS
VKGVIARQISKTPSNWRHEISFENWLKDENVVGIHGIDTRLLVRHIRESGSLNGIISSESKYSIAELFSLLKKSPSMSGL
NLVDKVTTKSAFNANSSCPVAFDMRIKNTQTIPYKVVAIDFGIKQSILDRLVAHGCEVTVLPASAEMTDVLALSPEGVFL
SNGPGDPSTVDSGINLAKDLIEYKKLPVFGICLGHQILGLALGGKTFKLSYGHRGLNHPCGLNGKVEITSQNHGFALNSE
SLNSEKIKITRLNLNDQTVAAISVIDRPFFGVQYHPEASPGPHDADHHFNHFVTLIEERRRIVD
>Mature_384_residues
MFFDTDSSAILLLEDGTYFEGLSFGAVGTISGEVVFNTGMTGYQEVITDPSYFGQLITFTYPEIGNTGVNYEDNESSHPS
VKGVIARQISKTPSNWRHEISFENWLKDENVVGIHGIDTRLLVRHIRESGSLNGIISSESKYSIAELFSLLKKSPSMSGL
NLVDKVTTKSAFNANSSCPVAFDMRIKNTQTIPYKVVAIDFGIKQSILDRLVAHGCEVTVLPASAEMTDVLALSPEGVFL
SNGPGDPSTVDSGINLAKDLIEYKKLPVFGICLGHQILGLALGGKTFKLSYGHRGLNHPCGLNGKVEITSQNHGFALNSE
SLNSEKIKITRLNLNDQTVAAISVIDRPFFGVQYHPEASPGPHDADHHFNHFVTLIEERRRIVD

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=37.9581151832461, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI21361331, Length=384, Percent_Identity=36.1979166666667, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI169790915, Length=384, Percent_Identity=36.1979166666667, Blast_Score=222, Evalue=5e-58,
Organism=Escherichia coli, GI1786215, Length=378, Percent_Identity=45.5026455026455, Blast_Score=329, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI193204318, Length=386, Percent_Identity=37.5647668393782, Blast_Score=223, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=36.6161616161616, Blast_Score=242, Evalue=7e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=389, Percent_Identity=38.560411311054, Blast_Score=240, Evalue=2e-64,
Organism=Drosophila melanogaster, GI45555749, Length=399, Percent_Identity=36.5914786967419, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24642586, Length=399, Percent_Identity=36.5914786967419, Blast_Score=228, Evalue=7e-60,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41975; Mature: 41975

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFDTDSSAILLLEDGTYFEGLSFGAVGTISGEVVFNTGMTGYQEVITDPSYFGQLITFT
CEECCCCCEEEEEECCCEECCCCCCEEEEECCEEEEECCCCCHHHHHCCHHHHCEEEEEE
YPEIGNTGVNYEDNESSHPSVKGVIARQISKTPSNWRHEISFENWLKDENVVGIHGIDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHCCCCCEEEEECCCHH
LLVRHIRESGSLNGIISSESKYSIAELFSLLKKSPSMSGLNLVDKVTTKSAFNANSSCPV
HHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCE
AFDMRIKNTQTIPYKVVAIDFGIKQSILDRLVAHGCEVTVLPASAEMTDVLALSPEGVFL
EEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCEEE
SNGPGDPSTVDSGINLAKDLIEYKKLPVFGICLGHQILGLALGGKTFKLSYGHRGLNHPC
ECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHEEEEEECCEEEEEECCCCCCCCCC
GLNGKVEITSQNHGFALNSESLNSEKIKITRLNLNDQTVAAISVIDRPFFGVQYHPEASP
CCCCEEEEEECCCCEEECCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCEEECCCCCC
GPHDADHHFNHFVTLIEERRRIVD
CCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFFDTDSSAILLLEDGTYFEGLSFGAVGTISGEVVFNTGMTGYQEVITDPSYFGQLITFT
CEECCCCCEEEEEECCCEECCCCCCEEEEECCEEEEECCCCCHHHHHCCHHHHCEEEEEE
YPEIGNTGVNYEDNESSHPSVKGVIARQISKTPSNWRHEISFENWLKDENVVGIHGIDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHCCCCCEEEEECCCHH
LLVRHIRESGSLNGIISSESKYSIAELFSLLKKSPSMSGLNLVDKVTTKSAFNANSSCPV
HHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCE
AFDMRIKNTQTIPYKVVAIDFGIKQSILDRLVAHGCEVTVLPASAEMTDVLALSPEGVFL
EEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECCCCEEE
SNGPGDPSTVDSGINLAKDLIEYKKLPVFGICLGHQILGLALGGKTFKLSYGHRGLNHPC
ECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHEEEEEECCEEEEEECCCCCCCCCC
GLNGKVEITSQNHGFALNSESLNSEKIKITRLNLNDQTVAAISVIDRPFFGVQYHPEASP
CCCCEEEEEECCCCEEECCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCEEECCCCCC
GPHDADHHFNHFVTLIEERRRIVD
CCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]