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The map label for this gene is nth [H]

Identifier: 124025549

GI number: 124025549

Start: 773737

End: 774390

Strand: Reverse

Name: nth [H]

Synonym: NATL1_08421

Alternate gene names: 124025549

Gene position: 774390-773737 (Counterclockwise)

Preceding gene: 124025552

Following gene: 124025548

Centisome position: 41.53

GC content: 30.58

Gene sequence:

>654_bases
ATGAAAAAAGATGAAAGGATAAAGATAATAATTAAGAGACTTGAAGAAATATATCCTGAGACACCGATACCATTAGATCA
TCAAAATGGTTTCACACTACTCGTAGCAGTTGTATTAAGTGCACAATCAACAGATAAGAAAGTCAATGAATTAACTAAAG
AACTTTTCAAGGTTGCTCCATCAGCTGAGAAAATGTATAAATTAGGTGAAAATAAAATATATAATTACATCAAACAACTT
GGACTTGCGAAAACTAAAGCAAAAAATACTCATAACTTATCAAAGATTATTTATGAAAAATTTAATAATATAATTCCAAA
TTCATTTCAAGAACTTGAGTCACTCCCTGGTGTCGGACATAAGACAGCTAGTGTTGTTATGTCTCAAGTATTTGGTGTAC
CATCATTTCCTGTCGATACACATATTCATAGATTATCTCAGAGATGGGGATTAACTTCAGGTAAAAATGTTATCCAAACG
GAAAAGGATCTAAAAAGATTATTTCCAAAAAATCTTTGGAATAAGTTACATTTGCAAATAATTTTTTACGGAAGAGAATA
TTGTTCAGCGAGAGGATGTAACGGCACAAACTGTAACTTATGTAAGGAACTTTACCCTAATAGAAAGAAAGCAGTTATTT
GTATAAAGGCTTAA

Upstream 100 bases:

>100_bases
AGTGTATAAAATGATACATGCTTGTTTTCGTTAAAGTCTATAGCTTCAAATTATAGGGTTTTAATTAAGTAACTTTATAA
ACAATGCAATTCATAATGCA

Downstream 100 bases:

>100_bases
TAATTATCTATTAATTTTATTATCGCAACAAAAAGGACTATTTCTTGCTAGAAACATATTAAAGAAATTTAATTCTAATG
AAATTAGCCATAACAGGAGC

Product: putative endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKKDERIKIIIKRLEEIYPETPIPLDHQNGFTLLVAVVLSAQSTDKKVNELTKELFKVAPSAEKMYKLGENKIYNYIKQL
GLAKTKAKNTHNLSKIIYEKFNNIIPNSFQELESLPGVGHKTASVVMSQVFGVPSFPVDTHIHRLSQRWGLTSGKNVIQT
EKDLKRLFPKNLWNKLHLQIIFYGREYCSARGCNGTNCNLCKELYPNRKKAVICIKA

Sequences:

>Translated_217_residues
MKKDERIKIIIKRLEEIYPETPIPLDHQNGFTLLVAVVLSAQSTDKKVNELTKELFKVAPSAEKMYKLGENKIYNYIKQL
GLAKTKAKNTHNLSKIIYEKFNNIIPNSFQELESLPGVGHKTASVVMSQVFGVPSFPVDTHIHRLSQRWGLTSGKNVIQT
EKDLKRLFPKNLWNKLHLQIIFYGREYCSARGCNGTNCNLCKELYPNRKKAVICIKA
>Mature_217_residues
MKKDERIKIIIKRLEEIYPETPIPLDHQNGFTLLVAVVLSAQSTDKKVNELTKELFKVAPSAEKMYKLGENKIYNYIKQL
GLAKTKAKNTHNLSKIIYEKFNNIIPNSFQELESLPGVGHKTASVVMSQVFGVPSFPVDTHIHRLSQRWGLTSGKNVIQT
EKDLKRLFPKNLWNKLHLQIIFYGREYCSARGCNGTNCNLCKELYPNRKKAVICIKA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=161, Percent_Identity=26.7080745341615, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=39.5121951219512, Blast_Score=141, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=30.8988764044944, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324530, Length=184, Percent_Identity=26.0869565217391, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6319304, Length=184, Percent_Identity=26.6304347826087, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=28.8888888888889, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24831; Mature: 24831

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDERIKIIIKRLEEIYPETPIPLDHQNGFTLLVAVVLSAQSTDKKVNELTKELFKVAP
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
SAEKMYKLGENKIYNYIKQLGLAKTKAKNTHNLSKIIYEKFNNIIPNSFQELESLPGVGH
CHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCH
KTASVVMSQVFGVPSFPVDTHIHRLSQRWGLTSGKNVIQTEKDLKRLFPKNLWNKLHLQI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
IFYGREYCSARGCNGTNCNLCKELYPNRKKAVICIKA
EEECHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MKKDERIKIIIKRLEEIYPETPIPLDHQNGFTLLVAVVLSAQSTDKKVNELTKELFKVAP
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
SAEKMYKLGENKIYNYIKQLGLAKTKAKNTHNLSKIIYEKFNNIIPNSFQELESLPGVGH
CHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCH
KTASVVMSQVFGVPSFPVDTHIHRLSQRWGLTSGKNVIQTEKDLKRLFPKNLWNKLHLQI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
IFYGREYCSARGCNGTNCNLCKELYPNRKKAVICIKA
EEECHHHHHCCCCCCCCHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]