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The map label for this gene is leuB [H]

Identifier: 124025533

GI number: 124025533

Start: 759278

End: 760357

Strand: Reverse

Name: leuB [H]

Synonym: NATL1_08261

Alternate gene names: 124025533

Gene position: 760357-759278 (Counterclockwise)

Preceding gene: 124025534

Following gene: 124025532

Centisome position: 40.78

GC content: 36.48

Gene sequence:

>1080_bases
ATGAAGTCTTACAAAATAACATTATTGCCAGGAGATGGAATTGGTCCAGAGATAACAAACGTCACTCATAAAATCCTTGA
CCTAGTCTCAAGGAAATTTGGCTTTGAAATCAAATTTAAAGAAATGCCTTTTGGTGGATCAGCAATAGATTCAGATGGTA
TCCCCTTCCCAGATAGAACTCTTCAAGAATGTAAGAACTCTGATGCTGTTTTATTAGCGGCAATAGGAGACCCAAAATAT
GACGAATTACCTAGAGAGAAAAGGCCTGAAACAGGCCTACTCAATCTCAGATCTTCTTTGGATCTTTTTGCAAATATAAG
GCCAGTAAAAATAATTCCATCCTTAACAAAAGCAAGTAGCTTGAAAGAAGACTTTGTTAAAAAAGTTGATTTAGTAGTAG
TTAGAGAACTTACCAGCGGTATTTACTTTGGAGAACCAAAAGGAAGGATAAAAACAGATAAAGGAGAAAGGGCCTTTAAC
ACGATGACATACACCTCCGAGGAAGTTAAACGAATAGCCGAAATTGCTTTTAAATTAGCCAAACAAAGAAATCAAAAAGT
TTGCTCAGTTGATAAAGCTAATGTTTTAGATGTAAGTCAACTATGGAGAGAGGAAACAATATTGGTGTCTAACAAATACA
AAGATATAGAACTTACGCATCAATATGTAGACAATGCAGCAATGCAACTTGTTAGAAATCCAAGCCAATTTGATGTGATT
CTTACAGGTAATTTATTTGGAGATATTCTTAGCGACATTGCAGCCATGCTGACAGGATCAATTGGCATGCTTCCTTCTGC
TTCGTTAACCACCGATGGGCCAGGTGTCTTTGAACCTGTTCATGGCTCAGCTCCTGACATCGCAGGAAAAGATATAGCTA
ATCCAATAGCAATGCTCTTATCTGCTGCAATGATGTTAAAAATTGCCCTTAATGAAAAAGAAGCAGCAACTTTTTTAGAA
AATGCAATTAACGAAATTCTTAATGACGGTTATAGAACTTCTGACTTAATGAGCAATCAGACGACTAAACAGGTAGGTTG
CTCTCAAATGGGTGAACTATTGGCGGAAAAATTAAAATAA

Upstream 100 bases:

>100_bases
GCTCTGCTAATTTCAAAAAACTACCTGAGATAGCAAAAGCTATTCGTCAATTAGATCGAAAAAAATCCAGGTAAGTTTTC
TACTTGATTAAGAACACAAA

Downstream 100 bases:

>100_bases
TTAGTTCACAAATTAAAAAACTCTTCGAATAGTTTCACTTTGAGGGTCAGTTGACCTGTAAAAATGAAAGTCAATACATA
AGCGTCGATGTCAAAGCTTC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKSYKITLLPGDGIGPEITNVTHKILDLVSRKFGFEIKFKEMPFGGSAIDSDGIPFPDRTLQECKNSDAVLLAAIGDPKY
DELPREKRPETGLLNLRSSLDLFANIRPVKIIPSLTKASSLKEDFVKKVDLVVVRELTSGIYFGEPKGRIKTDKGERAFN
TMTYTSEEVKRIAEIAFKLAKQRNQKVCSVDKANVLDVSQLWREETILVSNKYKDIELTHQYVDNAAMQLVRNPSQFDVI
LTGNLFGDILSDIAAMLTGSIGMLPSASLTTDGPGVFEPVHGSAPDIAGKDIANPIAMLLSAAMMLKIALNEKEAATFLE
NAINEILNDGYRTSDLMSNQTTKQVGCSQMGELLAEKLK

Sequences:

>Translated_359_residues
MKSYKITLLPGDGIGPEITNVTHKILDLVSRKFGFEIKFKEMPFGGSAIDSDGIPFPDRTLQECKNSDAVLLAAIGDPKY
DELPREKRPETGLLNLRSSLDLFANIRPVKIIPSLTKASSLKEDFVKKVDLVVVRELTSGIYFGEPKGRIKTDKGERAFN
TMTYTSEEVKRIAEIAFKLAKQRNQKVCSVDKANVLDVSQLWREETILVSNKYKDIELTHQYVDNAAMQLVRNPSQFDVI
LTGNLFGDILSDIAAMLTGSIGMLPSASLTTDGPGVFEPVHGSAPDIAGKDIANPIAMLLSAAMMLKIALNEKEAATFLE
NAINEILNDGYRTSDLMSNQTTKQVGCSQMGELLAEKLK
>Mature_359_residues
MKSYKITLLPGDGIGPEITNVTHKILDLVSRKFGFEIKFKEMPFGGSAIDSDGIPFPDRTLQECKNSDAVLLAAIGDPKY
DELPREKRPETGLLNLRSSLDLFANIRPVKIIPSLTKASSLKEDFVKKVDLVVVRELTSGIYFGEPKGRIKTDKGERAFN
TMTYTSEEVKRIAEIAFKLAKQRNQKVCSVDKANVLDVSQLWREETILVSNKYKDIELTHQYVDNAAMQLVRNPSQFDVI
LTGNLFGDILSDIAAMLTGSIGMLPSASLTTDGPGVFEPVHGSAPDIAGKDIANPIAMLLSAAMMLKIALNEKEAATFLE
NAINEILNDGYRTSDLMSNQTTKQVGCSQMGELLAEKLK

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=360, Percent_Identity=34.7222222222222, Blast_Score=174, Evalue=8e-44,
Organism=Homo sapiens, GI28178821, Length=372, Percent_Identity=30.6451612903226, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI28178816, Length=355, Percent_Identity=30.9859154929577, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI4758582, Length=352, Percent_Identity=30.1136363636364, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI28178838, Length=314, Percent_Identity=31.8471337579618, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI28178819, Length=193, Percent_Identity=35.2331606217617, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=49.7191011235955, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1788101, Length=355, Percent_Identity=36.056338028169, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1787381, Length=406, Percent_Identity=27.5862068965517, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=347, Percent_Identity=34.5821325648415, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17550882, Length=346, Percent_Identity=32.3699421965318, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=337, Percent_Identity=29.673590504451, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=349, Percent_Identity=30.3724928366762, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319830, Length=355, Percent_Identity=44.5070422535211, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=37.6044568245125, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=351, Percent_Identity=32.4786324786325, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=358, Percent_Identity=31.5642458100559, Blast_Score=129, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24643268, Length=360, Percent_Identity=35.8333333333333, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24643270, Length=360, Percent_Identity=35.8333333333333, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24661184, Length=371, Percent_Identity=32.0754716981132, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI161078635, Length=347, Percent_Identity=29.6829971181556, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI161078637, Length=347, Percent_Identity=29.6829971181556, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI161078633, Length=347, Percent_Identity=29.6829971181556, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24650122, Length=347, Percent_Identity=29.6829971181556, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI281362242, Length=346, Percent_Identity=32.0809248554913, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648872, Length=346, Percent_Identity=32.0809248554913, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078639, Length=343, Percent_Identity=29.7376093294461, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI20130355, Length=369, Percent_Identity=24.390243902439, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39475; Mature: 39475

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYKITLLPGDGIGPEITNVTHKILDLVSRKFGFEIKFKEMPFGGSAIDSDGIPFPDRT
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHH
LQECKNSDAVLLAAIGDPKYDELPREKRPETGLLNLRSSLDLFANIRPVKIIPSLTKASS
HHHHCCCCCEEEEECCCCCHHHCCHHCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHH
LKEDFVKKVDLVVVRELTSGIYFGEPKGRIKTDKGERAFNTMTYTSEEVKRIAEIAFKLA
HHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
KQRNQKVCSVDKANVLDVSQLWREETILVSNKYKDIELTHQYVDNAAMQLVRNPSQFDVI
HHHCCCEECCCCCCCCCHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
LTGNLFGDILSDIAAMLTGSIGMLPSASLTTDGPGVFEPVHGSAPDIAGKDIANPIAMLL
EECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SAAMMLKIALNEKEAATFLENAINEILNDGYRTSDLMSNQTTKQVGCSQMGELLAEKLK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSYKITLLPGDGIGPEITNVTHKILDLVSRKFGFEIKFKEMPFGGSAIDSDGIPFPDRT
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHH
LQECKNSDAVLLAAIGDPKYDELPREKRPETGLLNLRSSLDLFANIRPVKIIPSLTKASS
HHHHCCCCCEEEEECCCCCHHHCCHHCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHH
LKEDFVKKVDLVVVRELTSGIYFGEPKGRIKTDKGERAFNTMTYTSEEVKRIAEIAFKLA
HHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
KQRNQKVCSVDKANVLDVSQLWREETILVSNKYKDIELTHQYVDNAAMQLVRNPSQFDVI
HHHCCCEECCCCCCCCCHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
LTGNLFGDILSDIAAMLTGSIGMLPSASLTTDGPGVFEPVHGSAPDIAGKDIANPIAMLL
EECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SAAMMLKIALNEKEAATFLENAINEILNDGYRTSDLMSNQTTKQVGCSQMGELLAEKLK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA