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The map label for this gene is glnA [H]

Identifier: 124025480

GI number: 124025480

Start: 705143

End: 706564

Strand: Reverse

Name: glnA [H]

Synonym: NATL1_07731

Alternate gene names: 124025480

Gene position: 706564-705143 (Counterclockwise)

Preceding gene: 124025482

Following gene: 124025479

Centisome position: 37.89

GC content: 40.3

Gene sequence:

>1422_bases
ATGAGCAAGACCTCTCAAGATGTTCTTCGTCAAATTAAGGATGAGGGCATTGAGCTAATAGATTTAAAATTCACTGACTT
ACATGGTAAGTGGCAGCATTTAACAGTAGCTTCAGATCTAATTGAAGAAAGCTCTTTTACAGAAGGGCTTGCTTTTGATG
GCTCTTCAATTCGCGGTTGGAAAGCCATAAACGAATCTGATATGGCAATGGTTCCAGATCCATCAACCAGCTGGATAGAC
CCTTTTTACCATCACAAAACTCTTAGTTTAATTTGCTCAATTCAGGAGCCAAGGAGTGGAGAGCCATATGCCAGATGCCC
AAGAGCTTTGGCGCAAAAGGCTTTGGATTACTTAGGAAGCACAAGTGTTGCAGATGCTGCATTTTTTGGTCCTGAACCAG
AATTCTTTATTTTTGATGATGTCCGATACAACTCAGGAGAAGGTAGTAGCTTTTATAGCGTTGACACCATAGAAGCTCCC
TGGAATTCAGGGAGAGTAGAAGAAGGAGGAAACCTTGGGTACAAAATTCAACTAAAAGAGGGATATTTCCCAGTTTCACC
TAATGACACAGCCCAAGATATGAGGTCAGAGATGCTTCTACTGATGGGTGAGTTGGGTATACCCATTGAGAAGCATCACC
ATGAAGTAGCAGGAGCTGGTCAACATGAATTAGGAATGAAATTTGCTCCCTTAATCAACGCAGCTGACAATGTAATGATT
TACAAATACATCGTCAGAAACGTAGCAAAGAAATATGGGAAAACTGCAACTTTCATGCCCAAGCCAGTCTTCAATGACAA
TGGGACAGGAATGCATGTTCATCAAAGTCTTTGGAAAGGAGGTCAACCTTTATTTTATGGAGAAGGTACTTACGCTAATT
TATCTCAAACGGCTAAGTGGTATATCGGTGGAATACTCAAACATGCACCGTCTTTCCTTGCTTTTACCAATCCAACTACC
AACAGTTACAAAAGATTGGTTCCAGGATTCGAAGCACCAGTTAATTTAGTTTACTCACAGGGCAATCGATCAGCTGCTGT
AAGAATACCTTTGACTGGACCAAGTCCAAAAGCAAAGAGACTTGAATTTAGATCAGGTGACGCATTAGCTAACCCTTACA
TTGCTTTTTCAGCAATGATGATGGCAGGTATCGATGGAATCAAAAATCAAATTGATCCTGGAGATGGGGTTGACGTTGAC
CTTTTTGAGCTTCCTTCAGAGGAATTATCAAAAATAGACACTGTCCCCTCTTCATTGAACGATGCACTAGAGGCATTAAA
AAATGACAGCAAATATCTAACTGAAGGTGGAGTATTTACAGAAGATTTCATTAATAACTGGATAGAACTTAAATACGAAG
AAGTTCAACAGCTTAGACAAAGGCCTCATCCTCATGAATTTACTATGTACTACGACGCTTAA

Upstream 100 bases:

>100_bases
TTCTGTTAAAAGTCTAAAAAAAGTGCGTCTTAATACAAAAAGAGATGCGATCGCTCATTACGTTCAAAATTAAGTATCTC
TGTTGTAAATCAAATTCATC

Downstream 100 bases:

>100_bases
AATAAATTCCTTTAATAATTTTCAGGATACAAAATTAAGGTCTATGCATAAATATGCATAGACCTTAATTTTTTTTTAGG
TAACTCGTCACTAATCAATC

Product: glutamine synthetase, glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSKTSQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVASDLIEESSFTEGLAFDGSSIRGWKAINESDMAMVPDPSTSWID
PFYHHKTLSLICSIQEPRSGEPYARCPRALAQKALDYLGSTSVADAAFFGPEPEFFIFDDVRYNSGEGSSFYSVDTIEAP
WNSGRVEEGGNLGYKIQLKEGYFPVSPNDTAQDMRSEMLLLMGELGIPIEKHHHEVAGAGQHELGMKFAPLINAADNVMI
YKYIVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKGGQPLFYGEGTYANLSQTAKWYIGGILKHAPSFLAFTNPTT
NSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYIAFSAMMMAGIDGIKNQIDPGDGVDVD
LFELPSEELSKIDTVPSSLNDALEALKNDSKYLTEGGVFTEDFINNWIELKYEEVQQLRQRPHPHEFTMYYDA

Sequences:

>Translated_473_residues
MSKTSQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVASDLIEESSFTEGLAFDGSSIRGWKAINESDMAMVPDPSTSWID
PFYHHKTLSLICSIQEPRSGEPYARCPRALAQKALDYLGSTSVADAAFFGPEPEFFIFDDVRYNSGEGSSFYSVDTIEAP
WNSGRVEEGGNLGYKIQLKEGYFPVSPNDTAQDMRSEMLLLMGELGIPIEKHHHEVAGAGQHELGMKFAPLINAADNVMI
YKYIVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKGGQPLFYGEGTYANLSQTAKWYIGGILKHAPSFLAFTNPTT
NSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYIAFSAMMMAGIDGIKNQIDPGDGVDVD
LFELPSEELSKIDTVPSSLNDALEALKNDSKYLTEGGVFTEDFINNWIELKYEEVQQLRQRPHPHEFTMYYDA
>Mature_472_residues
SKTSQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVASDLIEESSFTEGLAFDGSSIRGWKAINESDMAMVPDPSTSWIDP
FYHHKTLSLICSIQEPRSGEPYARCPRALAQKALDYLGSTSVADAAFFGPEPEFFIFDDVRYNSGEGSSFYSVDTIEAPW
NSGRVEEGGNLGYKIQLKEGYFPVSPNDTAQDMRSEMLLLMGELGIPIEKHHHEVAGAGQHELGMKFAPLINAADNVMIY
KYIVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKGGQPLFYGEGTYANLSQTAKWYIGGILKHAPSFLAFTNPTTN
SYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPSPKAKRLEFRSGDALANPYIAFSAMMMAGIDGIKNQIDPGDGVDVDL
FELPSEELSKIDTVPSSLNDALEALKNDSKYLTEGGVFTEDFINNWIELKYEEVQQLRQRPHPHEFTMYYDA

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=497, Percent_Identity=22.9376257545272, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=52.3504273504274, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI87081870, Length=178, Percent_Identity=32.5842696629214, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52656; Mature: 52525

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTSQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVASDLIEESSFTEGLAFDGSSIRGW
CCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCCCCEEECCCCCCCC
KAINESDMAMVPDPSTSWIDPFYHHKTLSLICSIQEPRSGEPYARCPRALAQKALDYLGS
CCCCCCCCEECCCCCCCCCCHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
TSVADAAFFGPEPEFFIFDDVRYNSGEGSSFYSVDTIEAPWNSGRVEEGGNLGYKIQLKE
CCHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECC
GYFPVSPNDTAQDMRSEMLLLMGELGIPIEKHHHEVAGAGQHELGMKFAPLINAADNVMI
CEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHCHHHCCCCCHHHCCCHHHHHCCCCCHHH
YKYIVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKGGQPLFYGEGTYANLSQTAKW
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHH
YIGGILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPSPKAKR
HHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHE
LEFRSGDALANPYIAFSAMMMAGIDGIKNQIDPGDGVDVDLFELPSEELSKIDTVPSSLN
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCHHHHHHHHCCHHHHH
DALEALKNDSKYLTEGGVFTEDFINNWIELKYEEVQQLRQRPHPHEFTMYYDA
HHHHHHHCCCCEEECCCCCHHHHHHHHHEECHHHHHHHHHCCCCCEEEEEECC
>Mature Secondary Structure 
SKTSQDVLRQIKDEGIELIDLKFTDLHGKWQHLTVASDLIEESSFTEGLAFDGSSIRGW
CCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEHHHHHHHHHCCCCCCEEECCCCCCCC
KAINESDMAMVPDPSTSWIDPFYHHKTLSLICSIQEPRSGEPYARCPRALAQKALDYLGS
CCCCCCCCEECCCCCCCCCCHHHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCC
TSVADAAFFGPEPEFFIFDDVRYNSGEGSSFYSVDTIEAPWNSGRVEEGGNLGYKIQLKE
CCHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECC
GYFPVSPNDTAQDMRSEMLLLMGELGIPIEKHHHEVAGAGQHELGMKFAPLINAADNVMI
CEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHCHHHCCCCCHHHCCCHHHHHCCCCCHHH
YKYIVRNVAKKYGKTATFMPKPVFNDNGTGMHVHQSLWKGGQPLFYGEGTYANLSQTAKW
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHH
YIGGILKHAPSFLAFTNPTTNSYKRLVPGFEAPVNLVYSQGNRSAAVRIPLTGPSPKAKR
HHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHE
LEFRSGDALANPYIAFSAMMMAGIDGIKNQIDPGDGVDVDLFELPSEELSKIDTVPSSLN
EEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCHHHHHHHHCCHHHHH
DALEALKNDSKYLTEGGVFTEDFINNWIELKYEEVQQLRQRPHPHEFTMYYDA
HHHHHHHCCCCEEECCCCCHHHHHHHHHEECHHHHHHHHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678591 [H]