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The map label for this gene is ispG [H]

Identifier: 124025441

GI number: 124025441

Start: 676176

End: 677399

Strand: Direct

Name: ispG [H]

Synonym: NATL1_07341

Alternate gene names: 124025441

Gene position: 676176-677399 (Clockwise)

Preceding gene: 124025440

Following gene: 124025443

Centisome position: 36.26

GC content: 37.83

Gene sequence:

>1224_bases
ATGATTGCGACCCTAGAAAAAAATATGGAAGGAAATAATCTCTCTCAGCGATATAGCACCAGAATTATTCGTAGAGATAC
TAGGCCAGTAATGGTCGGTGATATTGGCATCGGTGGAGATAATCCAGTGCGTGTTCAATCAATGATTAATGAAGATACGA
TGGATATCGAGGGTTCAACGGCCGCAATAAGGAGATTGCATGAAGTTGGATGTGAGATCGTCAGATTAACAGTGCCAACT
CTTGCAAGTGCAAAAGCTGTGGGGGAAATCAAGAAACTTCTAGCTAGCACTTATCAACCAGTTCCTTTAGTAGCCGATGT
TCATCATAATGGAATGAAAATAGCCTTAGAAGTAGCTAAGCATGTAGATAAAGTTCGTATCAACCCTGGATTATTCGTTT
TTGAAAAACCTGATCCAAATAGAACTGAATTTACTAAAGATGAAATTGATGTAATTAAAGAGAAGATCATACAAAAATTC
AAACCAATTGTTAATACTTTAAAAGAGCAAAATAAGGCACTCAGAATAGGCGTTAACCATGGATCTTTGTCTGAAAGAAT
GTTATTTGCTTATGGAGATACTCCATTTGGAATGGTTGAATCAGCTATGGAATTTATTCGAATATGTCATTCATTAGATT
TTCATAATATTGTAATTTCGATGAAGGCTTCTCGAGCTCCCGTGATGCTTGCAGCTTATAGAATGATGGCTGACACAATG
GACAAAGAGGGATTTAATTATCCTCTGCATTTAGGTGTAACGGAAGCGGGAGACGGGGATTATGGAAGAATTAAAAGTAC
GGTAGGGATAGGGACATTATTATCCGAAGGTATTGGAGATACCATTAGAGTTTCTTTAACAGAGGCGCCCGAAAAAGAAA
TACCAGTTGCATATTCAATTTTACAAGCGGTTGGTTTGAGAAAAACCATGGTTGAATATATTAGTTGTCCTAGTTGTGGT
AGAACATTATTTAATTTAGAGGAAGTTGTAGCAAGAGTTAGAGACGCTACTCAACATTTAACCGGTTTGGATATTGCTGT
AATGGGTTGCATCGTTAATGGGCCTGGAGAGATGGCAGATGCTGATTATGGTTATGTAGGAAAAGGTGTTGGAACCATTG
CTCTTTATAGGAATAGAGATGAAATTAAGAGGGTACCTGAGGATGAAGGCGTTCAGGCATTGGTTGATTTAATTAAAGAG
GATGGTAAATGGGTAGATCCTTAA

Upstream 100 bases:

>100_bases
ACTAAATCTGATTTTTTAAAAATTAAGGAAAAAATTGATTTTTTATAATAGCCCTTAAATATGTCATTCTTATTTATTAA
AGAGGCTCAATTGTTAACCC

Downstream 100 bases:

>100_bases
AATAGAATATATCTTTTTATTGTTTTAGTATTAGAAAAATTATTTGATAATTATGTTGAAGAGATTTTTAAAAATATGTT
TACTTATAGCTATTTGCCCT

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MIATLEKNMEGNNLSQRYSTRIIRRDTRPVMVGDIGIGGDNPVRVQSMINEDTMDIEGSTAAIRRLHEVGCEIVRLTVPT
LASAKAVGEIKKLLASTYQPVPLVADVHHNGMKIALEVAKHVDKVRINPGLFVFEKPDPNRTEFTKDEIDVIKEKIIQKF
KPIVNTLKEQNKALRIGVNHGSLSERMLFAYGDTPFGMVESAMEFIRICHSLDFHNIVISMKASRAPVMLAAYRMMADTM
DKEGFNYPLHLGVTEAGDGDYGRIKSTVGIGTLLSEGIGDTIRVSLTEAPEKEIPVAYSILQAVGLRKTMVEYISCPSCG
RTLFNLEEVVARVRDATQHLTGLDIAVMGCIVNGPGEMADADYGYVGKGVGTIALYRNRDEIKRVPEDEGVQALVDLIKE
DGKWVDP

Sequences:

>Translated_407_residues
MIATLEKNMEGNNLSQRYSTRIIRRDTRPVMVGDIGIGGDNPVRVQSMINEDTMDIEGSTAAIRRLHEVGCEIVRLTVPT
LASAKAVGEIKKLLASTYQPVPLVADVHHNGMKIALEVAKHVDKVRINPGLFVFEKPDPNRTEFTKDEIDVIKEKIIQKF
KPIVNTLKEQNKALRIGVNHGSLSERMLFAYGDTPFGMVESAMEFIRICHSLDFHNIVISMKASRAPVMLAAYRMMADTM
DKEGFNYPLHLGVTEAGDGDYGRIKSTVGIGTLLSEGIGDTIRVSLTEAPEKEIPVAYSILQAVGLRKTMVEYISCPSCG
RTLFNLEEVVARVRDATQHLTGLDIAVMGCIVNGPGEMADADYGYVGKGVGTIALYRNRDEIKRVPEDEGVQALVDLIKE
DGKWVDP
>Mature_407_residues
MIATLEKNMEGNNLSQRYSTRIIRRDTRPVMVGDIGIGGDNPVRVQSMINEDTMDIEGSTAAIRRLHEVGCEIVRLTVPT
LASAKAVGEIKKLLASTYQPVPLVADVHHNGMKIALEVAKHVDKVRINPGLFVFEKPDPNRTEFTKDEIDVIKEKIIQKF
KPIVNTLKEQNKALRIGVNHGSLSERMLFAYGDTPFGMVESAMEFIRICHSLDFHNIVISMKASRAPVMLAAYRMMADTM
DKEGFNYPLHLGVTEAGDGDYGRIKSTVGIGTLLSEGIGDTIRVSLTEAPEKEIPVAYSILQAVGLRKTMVEYISCPSCG
RTLFNLEEVVARVRDATQHLTGLDIAVMGCIVNGPGEMADADYGYVGKGVGTIALYRNRDEIKRVPEDEGVQALVDLIKE
DGKWVDP

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=391, Percent_Identity=36.0613810741688, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 44857; Mature: 44857

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATLEKNMEGNNLSQRYSTRIIRRDTRPVMVGDIGIGGDNPVRVQSMINEDTMDIEGST
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCHH
AAIRRLHEVGCEIVRLTVPTLASAKAVGEIKKLLASTYQPVPLVADVHHNGMKIALEVAK
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
HVDKVRINPGLFVFEKPDPNRTEFTKDEIDVIKEKIIQKFKPIVNTLKEQNKALRIGVNH
HHHHEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
GSLSERMLFAYGDTPFGMVESAMEFIRICHSLDFHNIVISMKASRAPVMLAAYRMMADTM
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
DKEGFNYPLHLGVTEAGDGDYGRIKSTVGIGTLLSEGIGDTIRVSLTEAPEKEIPVAYSI
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHH
LQAVGLRKTMVEYISCPSCGRTLFNLEEVVARVRDATQHLTGLDIAVMGCIVNGPGEMAD
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
ADYGYVGKGVGTIALYRNRDEIKRVPEDEGVQALVDLIKEDGKWVDP
CCCCCCCCCCEEEEEEECHHHHHCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIATLEKNMEGNNLSQRYSTRIIRRDTRPVMVGDIGIGGDNPVRVQSMINEDTMDIEGST
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCCCCHH
AAIRRLHEVGCEIVRLTVPTLASAKAVGEIKKLLASTYQPVPLVADVHHNGMKIALEVAK
HHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHH
HVDKVRINPGLFVFEKPDPNRTEFTKDEIDVIKEKIIQKFKPIVNTLKEQNKALRIGVNH
HHHHEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
GSLSERMLFAYGDTPFGMVESAMEFIRICHSLDFHNIVISMKASRAPVMLAAYRMMADTM
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
DKEGFNYPLHLGVTEAGDGDYGRIKSTVGIGTLLSEGIGDTIRVSLTEAPEKEIPVAYSI
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHH
LQAVGLRKTMVEYISCPSCGRTLFNLEEVVARVRDATQHLTGLDIAVMGCIVNGPGEMAD
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
ADYGYVGKGVGTIALYRNRDEIKRVPEDEGVQALVDLIKEDGKWVDP
CCCCCCCCCCEEEEEEECHHHHHCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]