Click here to switch to the map view.

The map label for this gene is phnE [H]

Identifier: 124025435

GI number: 124025435

Start: 670675

End: 672225

Strand: Reverse

Name: phnE [H]

Synonym: NATL1_07281

Alternate gene names: 124025435

Gene position: 672225-670675 (Counterclockwise)

Preceding gene: 124025436

Following gene: 124025434

Centisome position: 36.05

GC content: 30.63

Gene sequence:

>1551_bases
ATGAAATTAATAAAACCAGTATCAACTTTATTAACATTAATTCCATCAATAGCCTATATTCCAATTCTAATAGAAATCAT
CAAAGGATTTCATATTGGGGGTCTAAATATCATATTTCAATTCTTAAGCTCAGCAATAAAACCATCGTTTAATCAAGAAG
TAGTAAAAAGCGCATGGGAAGGACTTCAAATAACAATTGCTACTGCTTTAACTAGCTGGGTTATAAGTATGCTTATTGGA
ATTGTTTTAGGTGTGCTATCTACAGATTTATTTTGGAAAAGTATTCCTAAATTTTCTTATTTAGGTAAATTCATAAAATA
CTCATTAGCAATTCCAAGATCAATACATGAAGTAGTTTGGGGCTTGCTATTTATTCAAATTTTAGGTTTAAATATTTGGG
TGGCAATTATATCTATAGTCATTCCCTATTCAGCCTTAACAGCAAGAGTAATTTCAGAGCAACTAGATAGTTTTGACATA
CAACCTCTAATAGCAATTAAGCAAACTGGATCTAATATTATGGGCTCATTTATAACTATTCTACTACCAAAGTTAATACC
TATCGTATCTACATATGGAAGTTACAGATTTGAATGTGCAATTAGGGGTGTAACATTACTTGGAATATTTGGATTAGGAG
GTATAGGAACAGAACTATATTTAACTTTAAAATCCTTTGAGTTCAATGAGATGTGGACTTGTTTGTGGATGCTTTGGTTA
GTAATAATTTTATTAGAGAAATTAATAAGATTTTCTAGAAGTTATTTATCTGACAATATTAGTTTAAAAAATTCTTTCTT
AATTTCAATCTCAATATTTACACTATCTCTATCGTTAGGTATTGGTTGGCTTTATAATCTAAATTTTGAGTTATTTACTC
CATTAAGCTTTTCGTATTTAAATTTACCTTCATTCGTAGAGATAAGAAATGGATTTAATAACTTACCTCTGTTTAAACTA
ATTATTACAACAATTTTAATAACTTTATTTGCCTCTGGGATTGCAATAGGGACACCTCCACTTTTACTAGTATTATTCCC
AGGTAAATTCTCTCTAAAAGTTCAAAATCTTATTTGGATCTTCTTTAGATTGATACCTCCTCCATTGACCACTATACTTA
TTCTTCTTTTTACTAATCCTAGTATATCTGTAGCCGCATTATCACTTGGAATTACACATATGGGAGTCATGGGAAGGTTA
CTTACAGATAACATACTAAATCAAGAAAAAAGTATTTATGGAGCGATTAAAAGCAATGGTTCAAGCAAGCAATCAGCAAC
GCTTTATGGAATATTAGCCCCCCAAAGTAATAGTTACTTAGCTTATGGAGCATACAGAAGTGACGTAATCCTAAAGGAAA
CAGCAATTATAGGAGCAGTCGGAGGAGTAGGATTAGGGTGGCAATTACAGGAATCACTAAGCTCATTCGATTGGGCACAA
GTTATGATAATAACTGCTACTTTCTCTTTATTAACAATTTCAGGAGAATTTTTATTTAACACTTCTCAAAATTATTGGTT
AAACAATTCAACCAACAATTTCATTAGTTAG

Upstream 100 bases:

>100_bases
TTTCAATAGAGTTATTGGGTTAAAAGATGGAGAGATTGTAATAGATAGAGAAATTCATAATATTAATCAATCAGAGCTTG
ATTGGCTATTTTAATTCATA

Downstream 100 bases:

>100_bases
TTATTTTATTCAACATATAGACTTATGAATAAATATCAGAAAAAATTAATCATACTTGGAGATAGTGGGGTTTACGGATG
GGGAGATCTAGAAGGAGGCG

Product: putative phosphonate ABC transporter

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKLIKPVSTLLTLIPSIAYIPILIEIIKGFHIGGLNIIFQFLSSAIKPSFNQEVVKSAWEGLQITIATALTSWVISMLIG
IVLGVLSTDLFWKSIPKFSYLGKFIKYSLAIPRSIHEVVWGLLFIQILGLNIWVAIISIVIPYSALTARVISEQLDSFDI
QPLIAIKQTGSNIMGSFITILLPKLIPIVSTYGSYRFECAIRGVTLLGIFGLGGIGTELYLTLKSFEFNEMWTCLWMLWL
VIILLEKLIRFSRSYLSDNISLKNSFLISISIFTLSLSLGIGWLYNLNFELFTPLSFSYLNLPSFVEIRNGFNNLPLFKL
IITTILITLFASGIAIGTPPLLLVLFPGKFSLKVQNLIWIFFRLIPPPLTTILILLFTNPSISVAALSLGITHMGVMGRL
LTDNILNQEKSIYGAIKSNGSSKQSATLYGILAPQSNSYLAYGAYRSDVILKETAIIGAVGGVGLGWQLQESLSSFDWAQ
VMIITATFSLLTISGEFLFNTSQNYWLNNSTNNFIS

Sequences:

>Translated_516_residues
MKLIKPVSTLLTLIPSIAYIPILIEIIKGFHIGGLNIIFQFLSSAIKPSFNQEVVKSAWEGLQITIATALTSWVISMLIG
IVLGVLSTDLFWKSIPKFSYLGKFIKYSLAIPRSIHEVVWGLLFIQILGLNIWVAIISIVIPYSALTARVISEQLDSFDI
QPLIAIKQTGSNIMGSFITILLPKLIPIVSTYGSYRFECAIRGVTLLGIFGLGGIGTELYLTLKSFEFNEMWTCLWMLWL
VIILLEKLIRFSRSYLSDNISLKNSFLISISIFTLSLSLGIGWLYNLNFELFTPLSFSYLNLPSFVEIRNGFNNLPLFKL
IITTILITLFASGIAIGTPPLLLVLFPGKFSLKVQNLIWIFFRLIPPPLTTILILLFTNPSISVAALSLGITHMGVMGRL
LTDNILNQEKSIYGAIKSNGSSKQSATLYGILAPQSNSYLAYGAYRSDVILKETAIIGAVGGVGLGWQLQESLSSFDWAQ
VMIITATFSLLTISGEFLFNTSQNYWLNNSTNNFIS
>Mature_516_residues
MKLIKPVSTLLTLIPSIAYIPILIEIIKGFHIGGLNIIFQFLSSAIKPSFNQEVVKSAWEGLQITIATALTSWVISMLIG
IVLGVLSTDLFWKSIPKFSYLGKFIKYSLAIPRSIHEVVWGLLFIQILGLNIWVAIISIVIPYSALTARVISEQLDSFDI
QPLIAIKQTGSNIMGSFITILLPKLIPIVSTYGSYRFECAIRGVTLLGIFGLGGIGTELYLTLKSFEFNEMWTCLWMLWL
VIILLEKLIRFSRSYLSDNISLKNSFLISISIFTLSLSLGIGWLYNLNFELFTPLSFSYLNLPSFVEIRNGFNNLPLFKL
IITTILITLFASGIAIGTPPLLLVLFPGKFSLKVQNLIWIFFRLIPPPLTTILILLFTNPSISVAALSLGITHMGVMGRL
LTDNILNQEKSIYGAIKSNGSSKQSATLYGILAPQSNSYLAYGAYRSDVILKETAIIGAVGGVGLGWQLQESLSSFDWAQ
VMIITATFSLLTISGEFLFNTSQNYWLNNSTNNFIS

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 57209; Mature: 57209

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIKPVSTLLTLIPSIAYIPILIEIIKGFHIGGLNIIFQFLSSAIKPSFNQEVVKSAWE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHC
GLQITIATALTSWVISMLIGIVLGVLSTDLFWKSIPKFSYLGKFIKYSLAIPRSIHEVVW
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
GLLFIQILGLNIWVAIISIVIPYSALTARVISEQLDSFDIQPLIAIKQTGSNIMGSFITI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
LLPKLIPIVSTYGSYRFECAIRGVTLLGIFGLGGIGTELYLTLKSFEFNEMWTCLWMLWL
HHHHHHHHHHHCCCEEEEEEECCCCEEHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
VIILLEKLIRFSRSYLSDNISLKNSFLISISIFTLSLSLGIGWLYNLNFELFTPLSFSYL
HHHHHHHHHHHHHHHHCCCCEECCEEEEEEEEHHHHHHHCCEEEEECCEEEECCCCCHHC
NLPSFVEIRNGFNNLPLFKLIITTILITLFASGIAIGTPPLLLVLFPGKFSLKVQNLIWI
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHHHH
FFRLIPPPLTTILILLFTNPSISVAALSLGITHMGVMGRLLTDNILNQEKSIYGAIKSNG
HHHHCCCCHHHHEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
SSKQSATLYGILAPQSNSYLAYGAYRSDVILKETAIIGAVGGVGLGWQLQESLSSFDWAQ
CCCCCEEEEEEECCCCCCEEEEECCCCCEEEEHHHHHCCCCCCCCCHHHHHHHCCCCHHH
VMIITATFSLLTISGEFLFNTSQNYWLNNSTNNFIS
EEHHEEHHHHHEEECCEEEECCCCCEECCCCCCCCC
>Mature Secondary Structure
MKLIKPVSTLLTLIPSIAYIPILIEIIKGFHIGGLNIIFQFLSSAIKPSFNQEVVKSAWE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHC
GLQITIATALTSWVISMLIGIVLGVLSTDLFWKSIPKFSYLGKFIKYSLAIPRSIHEVVW
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHH
GLLFIQILGLNIWVAIISIVIPYSALTARVISEQLDSFDIQPLIAIKQTGSNIMGSFITI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
LLPKLIPIVSTYGSYRFECAIRGVTLLGIFGLGGIGTELYLTLKSFEFNEMWTCLWMLWL
HHHHHHHHHHHCCCEEEEEEECCCCEEHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
VIILLEKLIRFSRSYLSDNISLKNSFLISISIFTLSLSLGIGWLYNLNFELFTPLSFSYL
HHHHHHHHHHHHHHHHCCCCEECCEEEEEEEEHHHHHHHCCEEEEECCEEEECCCCCHHC
NLPSFVEIRNGFNNLPLFKLIITTILITLFASGIAIGTPPLLLVLFPGKFSLKVQNLIWI
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEHHHHHHHH
FFRLIPPPLTTILILLFTNPSISVAALSLGITHMGVMGRLLTDNILNQEKSIYGAIKSNG
HHHHCCCCHHHHEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
SSKQSATLYGILAPQSNSYLAYGAYRSDVILKETAIIGAVGGVGLGWQLQESLSSFDWAQ
CCCCCEEEEEEECCCCCCEEEEECCCCCEEEEHHHHHCCCCCCCCCHHHHHHHCCCCHHH
VMIITATFSLLTISGEFLFNTSQNYWLNNSTNNFIS
EEHHEEHHHHHEEECCEEEECCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]