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The map label for this gene is met17 [H]

Identifier: 124025414

GI number: 124025414

Start: 649332

End: 650690

Strand: Direct

Name: met17 [H]

Synonym: NATL1_07071

Alternate gene names: 124025414

Gene position: 649332-650690 (Clockwise)

Preceding gene: 124025413

Following gene: 124025415

Centisome position: 34.82

GC content: 37.45

Gene sequence:

>1359_bases
GTGGCTAAAAAGAAAATAGAGATAATTAATTCTTTGACTTCCCAACGTTTTGAAACTCTTCAATTGCATGCAGGTCAAGT
ACCAGATCCTGTTACCAATTCTAGAGCGGTTCCTATTTATCAGACAAGTTCATATGTTTTTAACGATGTAGATCATGGAG
CAAATTTATTTGGTTTGAAGGAATTTGGAAATATTTATACGCGCTTGATGAATCCTACAACGGATGTTTTCGAAAAAAGA
ATTGCAGCACTTGAAGGAGGAGTAGCTGCTTTAGCAACAGCATCAGGACAGTCTGCACAATTCATTGCGATTACAAACTT
CCTTACTGCAGGAGACAGCTTTGTATCAACATCGTTTTTGTATGGTGGCAGCTATAACCAATTCAAAGTTCAATTTCCAC
GATTAGGAATCAATGTCAAGTTTGCTGATGGTGATGATGCAGAAAGCTTTGAAGAACAAATTGATTCATCTACAAAAGCA
ATTTATGTAGAGTCAATGGGTAATCCTAGGTTTAACATCCCTGATTTTGATGGACTATCGAAATTAGCTAAATCGAAAAA
TATCCCCTTAATTGTAGATAACACTCTTGGTGCTGCTGGAGCTTTAATTCGTCCTATTGAACATGGCGCAGATGTGGTTG
TTCAAAGTGCTACAAAATGGATAGGAGGACATGGAACAAGTCTTGGAGGGGTAATTGTTGATGCAGGAACTTTTGATTGG
GGAAATGGAAAATATCCTTTAATGAGTCAACCAAGCGCGGCTTATCATGGTTTAGTTCATTGGGATGCTTTTGGATTTGG
GAGTGATATTTGTGGAATGCTTGGAGTCCCTACAGATCGAAACATTGCTTTTGCTTTGAGAGCTAGGTTAGAGGGGTTAC
GAGATTGGGGCCCTGCGATTAGTCCTTTTAATTCTTTTTTATTACTTCAAGGATTAGAAACTTTAAGTTTAAGAATAGAA
AGACATTGCTCTAATGCACTTGCATTGGCTAAGTGGTTAGACGATCATTCGAAAGTTGATAATGTTAGTTATCCAGGATT
ACCATCGGACAAATACCATTCAAGAGCCTCTAATTATATGACCAATAGAGGAAAAGGTTCTATGTTGATTTTCTCTCTGA
AAGGTGGCTTTGATGATGCTGTGAAGTTTATAAACTCTTTAAAACTTTCTAGTCATCTAGCAAATGTAGGCGATGCAAAA
ACATTAGTAATTCATCCTGCTTCAACAACTCATCAACAACTATCCCCAGAAGAACAGTTGTCCGCAGGTGTTACTCCAAC
TATGGTAAGAGTGTCTGTTGGTATAGAACATATTGATGATATCTTAGAGGATTTCGAGCAGGCTCTAAATTTAATTTAA

Upstream 100 bases:

>100_bases
CTTTTATCATTTATAAAAGAGAATTTACCATCTCTAGCTGAGTCAGATAAATCTTTACCTCCTAATGATTGGATAAATTA
ATAGCCAATTCTGAACTTAT

Downstream 100 bases:

>100_bases
TTTTCCAAGGAGGTATATAGATATGGCATTAATACTTCCTCGTAGTTATCATAAAATATCATCGATAGAAAAAAATCATA
TTTCATGGATTGAGCCTGAA

Product: putative O-Acetyl homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MAKKKIEIINSLTSQRFETLQLHAGQVPDPVTNSRAVPIYQTSSYVFNDVDHGANLFGLKEFGNIYTRLMNPTTDVFEKR
IAALEGGVAALATASGQSAQFIAITNFLTAGDSFVSTSFLYGGSYNQFKVQFPRLGINVKFADGDDAESFEEQIDSSTKA
IYVESMGNPRFNIPDFDGLSKLAKSKNIPLIVDNTLGAAGALIRPIEHGADVVVQSATKWIGGHGTSLGGVIVDAGTFDW
GNGKYPLMSQPSAAYHGLVHWDAFGFGSDICGMLGVPTDRNIAFALRARLEGLRDWGPAISPFNSFLLLQGLETLSLRIE
RHCSNALALAKWLDDHSKVDNVSYPGLPSDKYHSRASNYMTNRGKGSMLIFSLKGGFDDAVKFINSLKLSSHLANVGDAK
TLVIHPASTTHQQLSPEEQLSAGVTPTMVRVSVGIEHIDDILEDFEQALNLI

Sequences:

>Translated_452_residues
MAKKKIEIINSLTSQRFETLQLHAGQVPDPVTNSRAVPIYQTSSYVFNDVDHGANLFGLKEFGNIYTRLMNPTTDVFEKR
IAALEGGVAALATASGQSAQFIAITNFLTAGDSFVSTSFLYGGSYNQFKVQFPRLGINVKFADGDDAESFEEQIDSSTKA
IYVESMGNPRFNIPDFDGLSKLAKSKNIPLIVDNTLGAAGALIRPIEHGADVVVQSATKWIGGHGTSLGGVIVDAGTFDW
GNGKYPLMSQPSAAYHGLVHWDAFGFGSDICGMLGVPTDRNIAFALRARLEGLRDWGPAISPFNSFLLLQGLETLSLRIE
RHCSNALALAKWLDDHSKVDNVSYPGLPSDKYHSRASNYMTNRGKGSMLIFSLKGGFDDAVKFINSLKLSSHLANVGDAK
TLVIHPASTTHQQLSPEEQLSAGVTPTMVRVSVGIEHIDDILEDFEQALNLI
>Mature_451_residues
AKKKIEIINSLTSQRFETLQLHAGQVPDPVTNSRAVPIYQTSSYVFNDVDHGANLFGLKEFGNIYTRLMNPTTDVFEKRI
AALEGGVAALATASGQSAQFIAITNFLTAGDSFVSTSFLYGGSYNQFKVQFPRLGINVKFADGDDAESFEEQIDSSTKAI
YVESMGNPRFNIPDFDGLSKLAKSKNIPLIVDNTLGAAGALIRPIEHGADVVVQSATKWIGGHGTSLGGVIVDAGTFDWG
NGKYPLMSQPSAAYHGLVHWDAFGFGSDICGMLGVPTDRNIAFALRARLEGLRDWGPAISPFNSFLLLQGLETLSLRIER
HCSNALALAKWLDDHSKVDNVSYPGLPSDKYHSRASNYMTNRGKGSMLIFSLKGGFDDAVKFINSLKLSSHLANVGDAKT
LVIHPASTTHQQLSPEEQLSAGVTPTMVRVSVGIEHIDDILEDFEQALNLI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=439, Percent_Identity=30.5239179954442, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI25453487, Length=438, Percent_Identity=27.8538812785388, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI299473759, Length=439, Percent_Identity=27.5626423690205, Blast_Score=129, Evalue=4e-30,
Organism=Escherichia coli, GI1790375, Length=434, Percent_Identity=29.4930875576037, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789383, Length=440, Percent_Identity=20.9090909090909, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=454, Percent_Identity=30.1762114537445, Blast_Score=191, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17538055, Length=443, Percent_Identity=31.8284424379233, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=443, Percent_Identity=30.2483069977427, Blast_Score=167, Evalue=8e-42,
Organism=Saccharomyces cerevisiae, GI6323333, Length=451, Percent_Identity=50.9977827050998, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319307, Length=442, Percent_Identity=30.9954751131222, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6321254, Length=462, Percent_Identity=25.3246753246753, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321170, Length=369, Percent_Identity=25.4742547425474, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922554, Length=438, Percent_Identity=28.310502283105, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 48963; Mature: 48832

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKIEIINSLTSQRFETLQLHAGQVPDPVTNSRAVPIYQTSSYVFNDVDHGANLFGLK
CCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEECCCCEECCCCCCCCEEEHH
EFGNIYTRLMNPTTDVFEKRIAALEGGVAALATASGQSAQFIAITNFLTAGDSFVSTSFL
HHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHHHCCCCHHHHHEE
YGGSYNQFKVQFPRLGINVKFADGDDAESFEEQIDSSTKAIYVESMGNPRFNIPDFDGLS
ECCCCCEEEEEECEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHH
KLAKSKNIPLIVDNTLGAAGALIRPIEHGADVVVQSATKWIGGHGTSLGGVIVDAGTFDW
HHHHCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCEEEC
GNGKYPLMSQPSAAYHGLVHWDAFGFGSDICGMLGVPTDRNIAFALRARLEGLRDWGPAI
CCCCCCCCCCCCHHHCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
SPFNSFLLLQGLETLSLRIERHCSNALALAKWLDDHSKVDNVSYPGLPSDKYHSRASNYM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
TNRGKGSMLIFSLKGGFDDAVKFINSLKLSSHLANVGDAKTLVIHPASTTHQQLSPEEQL
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCHHHH
SAGVTPTMVRVSVGIEHIDDILEDFEQALNLI
HCCCCCEEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKKIEIINSLTSQRFETLQLHAGQVPDPVTNSRAVPIYQTSSYVFNDVDHGANLFGLK
CCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEEEECCCCEECCCCCCCCEEEHH
EFGNIYTRLMNPTTDVFEKRIAALEGGVAALATASGQSAQFIAITNFLTAGDSFVSTSFL
HHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEHHHHCCCCHHHHHEE
YGGSYNQFKVQFPRLGINVKFADGDDAESFEEQIDSSTKAIYVESMGNPRFNIPDFDGLS
ECCCCCEEEEEECEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHH
KLAKSKNIPLIVDNTLGAAGALIRPIEHGADVVVQSATKWIGGHGTSLGGVIVDAGTFDW
HHHHCCCCCEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEECCEEEC
GNGKYPLMSQPSAAYHGLVHWDAFGFGSDICGMLGVPTDRNIAFALRARLEGLRDWGPAI
CCCCCCCCCCCCHHHCCEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCC
SPFNSFLLLQGLETLSLRIERHCSNALALAKWLDDHSKVDNVSYPGLPSDKYHSRASNYM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
TNRGKGSMLIFSLKGGFDDAVKFINSLKLSSHLANVGDAKTLVIHPASTTHQQLSPEEQL
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCHHHH
SAGVTPTMVRVSVGIEHIDDILEDFEQALNLI
HCCCCCEEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]