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The map label for this gene is mas1 [H]

Identifier: 124025403

GI number: 124025403

Start: 637817

End: 638530

Strand: Reverse

Name: mas1 [H]

Synonym: NATL1_06941

Alternate gene names: 124025403

Gene position: 638530-637817 (Counterclockwise)

Preceding gene: 124025404

Following gene: 124025400

Centisome position: 34.24

GC content: 32.49

Gene sequence:

>714_bases
ATGAGAAAAATCCTTATAAGTGGAGCAAGTAGAGGTATAGGAAAAGCGATAGCAATAAAATTACTTAAAGAAGGACACTC
GTTAAGCTTAGGAGTAAGAGAAAGAGACGATTTATTAAATACACCATTAGATCCAAAAATTAACAATTCAGATAGTTTCT
TGGTTCACACCTATGATGCAACTGATCAAAACTCATCAAAGAAATGGGTAGACAAAACATTTGAAACATTTAAAAGTGTT
GATACTATTATTCATTGCGCTGGTATATTCAAAACAACAAAACTATTATTTAATGATAATGAAATGAAAGATATTGAAGA
TCTATGGAAAGTTAATGTGATGGGGCCATGGATACTAACAAAACATGCTTGGAAGTATTTATCTCTGAGCAATTCAGCAC
GAATTATTGTATTGGTATCAATGAGTGGAAAACGATCAAAAGGTAACTTGTGTGGCTATTCTATGAGTAAATTCGCTTTA
ATGAGCTTATGCCAAACAATGAGAAATGAAGGATGGGGAAACGGTATTAGAGTTACAGCTATTTGCCCAGGCTGGGTAAA
TACAGACATGGCAAAAGAAATAAAACATTATCCCAAAAAAGATATGTCACAAGCAAATGATATTGCAAGTATTTGCTCCA
ATTTACTTACTTTACCAAACAGTTCTATTCCTTTTGAAATCGCACTAAATTGTCAACTTGAAACAAACATTTAG

Upstream 100 bases:

>100_bases
AGATGTTTAGGAAATCACCCTGGACTATCAAACAAGGCTTAATGAATATGCATGGTAGAGAATTAAATTTATTATTTAAA
GCATTATTAGTTAATAATAA

Downstream 100 bases:

>100_bases
AAGATAAGTCTTGTTTTTTTTTAAATAGGATTAGTAGTTTTTTTAAATGTATTCAAAACTCCCAGAATTGCCTGACGGAG
ATACTTATGAAAATAGTTTC

Product: short-chain dehydrogenase/reductase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRKILISGASRGIGKAIAIKLLKEGHSLSLGVRERDDLLNTPLDPKINNSDSFLVHTYDATDQNSSKKWVDKTFETFKSV
DTIIHCAGIFKTTKLLFNDNEMKDIEDLWKVNVMGPWILTKHAWKYLSLSNSARIIVLVSMSGKRSKGNLCGYSMSKFAL
MSLCQTMRNEGWGNGIRVTAICPGWVNTDMAKEIKHYPKKDMSQANDIASICSNLLTLPNSSIPFEIALNCQLETNI

Sequences:

>Translated_237_residues
MRKILISGASRGIGKAIAIKLLKEGHSLSLGVRERDDLLNTPLDPKINNSDSFLVHTYDATDQNSSKKWVDKTFETFKSV
DTIIHCAGIFKTTKLLFNDNEMKDIEDLWKVNVMGPWILTKHAWKYLSLSNSARIIVLVSMSGKRSKGNLCGYSMSKFAL
MSLCQTMRNEGWGNGIRVTAICPGWVNTDMAKEIKHYPKKDMSQANDIASICSNLLTLPNSSIPFEIALNCQLETNI
>Mature_237_residues
MRKILISGASRGIGKAIAIKLLKEGHSLSLGVRERDDLLNTPLDPKINNSDSFLVHTYDATDQNSSKKWVDKTFETFKSV
DTIIHCAGIFKTTKLLFNDNEMKDIEDLWKVNVMGPWILTKHAWKYLSLSNSARIIVLVSMSGKRSKGNLCGYSMSKFAL
MSLCQTMRNEGWGNGIRVTAICPGWVNTDMAKEIKHYPKKDMSQANDIASICSNLLTLPNSSIPFEIALNCQLETNI

Specific function: Reduces deoxy-fructosyl-glutamine to mannopine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4503817, Length=200, Percent_Identity=27, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI15277342, Length=231, Percent_Identity=26.4069264069264, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787335, Length=233, Percent_Identity=25.7510729613734, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082100, Length=198, Percent_Identity=26.7676767676768, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=195, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=194, Percent_Identity=28.8659793814433, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=221, Percent_Identity=25.3393665158371, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=197, Percent_Identity=25.8883248730964, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=199, Percent_Identity=29.1457286432161, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6322861, Length=189, Percent_Identity=25.3968253968254, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648944, Length=227, Percent_Identity=27.3127753303965, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364494, Length=239, Percent_Identity=27.1966527196653, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571387, Length=248, Percent_Identity=28.2258064516129, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI23397609, Length=193, Percent_Identity=26.9430051813472, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644339, Length=188, Percent_Identity=26.5957446808511, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR013078
- InterPro:   IPR015845
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short; PF00300 PGAM [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 26503; Mature: 26503

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKILISGASRGIGKAIAIKLLKEGHSLSLGVRERDDLLNTPLDPKINNSDSFLVHTYDA
CCEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCCCCCCCEEEEEECC
TDQNSSKKWVDKTFETFKSVDTIIHCAGIFKTTKLLFNDNEMKDIEDLWKVNVMGPWILT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHEECCCCCCHHH
KHAWKYLSLSNSARIIVLVSMSGKRSKGNLCGYSMSKFALMSLCQTMRNEGWGNGIRVTA
HHHHHEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
ICPGWVNTDMAKEIKHYPKKDMSQANDIASICSNLLTLPNSSIPFEIALNCQLETNI
ECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MRKILISGASRGIGKAIAIKLLKEGHSLSLGVRERDDLLNTPLDPKINNSDSFLVHTYDA
CCEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCHHHHCCCCCCCCCCCCCEEEEEECC
TDQNSSKKWVDKTFETFKSVDTIIHCAGIFKTTKLLFNDNEMKDIEDLWKVNVMGPWILT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHEECCCCCCHHH
KHAWKYLSLSNSARIIVLVSMSGKRSKGNLCGYSMSKFALMSLCQTMRNEGWGNGIRVTA
HHHHHEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
ICPGWVNTDMAKEIKHYPKKDMSQANDIASICSNLLTLPNSSIPFEIALNCQLETNI
ECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA