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The map label for this gene is yafV [C]

Identifier: 124025382

GI number: 124025382

Start: 619936

End: 620760

Strand: Direct

Name: yafV [C]

Synonym: NATL1_06731

Alternate gene names: 124025382

Gene position: 619936-620760 (Clockwise)

Preceding gene: 124025381

Following gene: 124025383

Centisome position: 33.25

GC content: 41.21

Gene sequence:

>825_bases
GTGTCAGATTTTTTGGCTGCGGCCTTACAGCTAACAAGTACCTCAGACATAGACGCAAATCTCAATTCTGCTGAAGAACA
AATTGAGTTAGCGGCAAAGCGTGGGGCTGATCTTGTAGGACTTCCTGAAAACTTTGCATTTTTGGGTGAAGACCAGAAAA
AATTAAAAATTGCATCTTCAATATATGAAAAATGCAATAGTTTTTTAGTAACAATGGCAAGGAGATATCAAGTCGTTTTA
CTTGGAGGTGGTTTCCCTGTTCCCGCTGGCGATGGAGTAAGAACTTTAAATAGAGCGGAATTGTTTGGAAAAGACGGCCA
ATCTCTTGCAAGGTATGACAAGATTCATCTTTTTGATGTTGATCTACCTGAAGGAAATACTTATAGGGAATCTGAAACAA
TTGTTTCGGGAAGTGAATCTCCACCTGTAGTTGATGTGCCCGGTCTTTGCAAAATTGGATTATCTATTTGTTACGATGTG
CGATTCCCTGAGCTTTATCGGGATTTGGTAAATAAAGGCGCAGATTTATTAATGATTCCAGCTGCTTTTACTGCTTTTAC
GGGAAAGGATCATTGGCAAGTCTTGCTCCAAGCAAGAGCTATTGAAAACACTGCTTATGTTGTTGCACCAGCGCAAACTG
GTCGTCACTATGGCAGAAGACAAAGTCATGGGCATGCGATGGTAATTGATCCATGGGGGACAGTTTTAGCAGATGCAGGG
GTTGTTCAAGGAGCAGCAATAGCTCCTGCTGATAAAGAAAGAGTGGAGAGAATTAGAGGACAAATGCCCAGCCTGAAACA
TCGAAAAACAGAGCTTTTTATTTGA

Upstream 100 bases:

>100_bases
GTTGCGCTTCTTTGGATAACCTTTCTGTTATTCCAAAACAGATTGAGATGGGCGTACTTCGCTCAAACTAACTTTTAAAT
TATTTTTCTTTTTTAACTCA

Downstream 100 bases:

>100_bases
TGTTTAAAGGTCAATCTTTAATGTCAAAACTAGGATTGTTTGCGGGATTTATTTTTTGTTCCAATCTCTTTATTTCTTTA
CCTCTAAAATCTGCTAGTGC

Product: putative nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MSDFLAAALQLTSTSDIDANLNSAEEQIELAAKRGADLVGLPENFAFLGEDQKKLKIASSIYEKCNSFLVTMARRYQVVL
LGGGFPVPAGDGVRTLNRAELFGKDGQSLARYDKIHLFDVDLPEGNTYRESETIVSGSESPPVVDVPGLCKIGLSICYDV
RFPELYRDLVNKGADLLMIPAAFTAFTGKDHWQVLLQARAIENTAYVVAPAQTGRHYGRRQSHGHAMVIDPWGTVLADAG
VVQGAAIAPADKERVERIRGQMPSLKHRKTELFI

Sequences:

>Translated_274_residues
MSDFLAAALQLTSTSDIDANLNSAEEQIELAAKRGADLVGLPENFAFLGEDQKKLKIASSIYEKCNSFLVTMARRYQVVL
LGGGFPVPAGDGVRTLNRAELFGKDGQSLARYDKIHLFDVDLPEGNTYRESETIVSGSESPPVVDVPGLCKIGLSICYDV
RFPELYRDLVNKGADLLMIPAAFTAFTGKDHWQVLLQARAIENTAYVVAPAQTGRHYGRRQSHGHAMVIDPWGTVLADAG
VVQGAAIAPADKERVERIRGQMPSLKHRKTELFI
>Mature_273_residues
SDFLAAALQLTSTSDIDANLNSAEEQIELAAKRGADLVGLPENFAFLGEDQKKLKIASSIYEKCNSFLVTMARRYQVVLL
GGGFPVPAGDGVRTLNRAELFGKDGQSLARYDKIHLFDVDLPEGNTYRESETIVSGSESPPVVDVPGLCKIGLSICYDVR
FPELYRDLVNKGADLLMIPAAFTAFTGKDHWQVLLQARAIENTAYVVAPAQTGRHYGRRQSHGHAMVIDPWGTVLADAGV
VQGAAIAPADKERVERIRGQMPSLKHRKTELFI

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=273, Percent_Identity=39.1941391941392, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI297632348, Length=273, Percent_Identity=39.1941391941392, Blast_Score=185, Evalue=3e-47,
Organism=Homo sapiens, GI297632350, Length=273, Percent_Identity=39.1941391941392, Blast_Score=185, Evalue=3e-47,
Organism=Homo sapiens, GI9910460, Length=273, Percent_Identity=34.7985347985348, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI297632346, Length=195, Percent_Identity=36.9230769230769, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786412, Length=234, Percent_Identity=31.1965811965812, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556280, Length=270, Percent_Identity=40.3703703703704, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6322335, Length=300, Percent_Identity=34, Blast_Score=166, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323383, Length=274, Percent_Identity=34.6715328467153, Blast_Score=155, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17933642, Length=276, Percent_Identity=35.8695652173913, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21355835, Length=269, Percent_Identity=33.0855018587361, Blast_Score=148, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29945; Mature: 29814

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFLAAALQLTSTSDIDANLNSAEEQIELAAKRGADLVGLPENFAFLGEDQKKLKIASS
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCEECCCCHHHHHHHHH
IYEKCNSFLVTMARRYQVVLLGGGFPVPAGDGVRTLNRAELFGKDGQSLARYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCEEEEEEE
DLPEGNTYRESETIVSGSESPPVVDVPGLCKIGLSICYDVRFPELYRDLVNKGADLLMIP
ECCCCCCCCCCCCEECCCCCCCEECCCCHHHHHHHHHEECCCHHHHHHHHHCCCCEEEEC
AAFTAFTGKDHWQVLLQARAIENTAYVVAPAQTGRHYGRRQSHGHAMVIDPWGTVLADAG
CHHHHCCCCHHHHHHHHHHHCCCCEEEEEECHHCHHHCCCCCCCCEEEECCCHHHHHCCC
VVQGAAIAPADKERVERIRGQMPSLKHRKTELFI
HHCCCEECCCCHHHHHHHHCCCCCCHHCHHCCCC
>Mature Secondary Structure 
SDFLAAALQLTSTSDIDANLNSAEEQIELAAKRGADLVGLPENFAFLGEDQKKLKIASS
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCEECCCCHHHHHHHHH
IYEKCNSFLVTMARRYQVVLLGGGFPVPAGDGVRTLNRAELFGKDGQSLARYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHHHCEEEEEEE
DLPEGNTYRESETIVSGSESPPVVDVPGLCKIGLSICYDVRFPELYRDLVNKGADLLMIP
ECCCCCCCCCCCCEECCCCCCCEECCCCHHHHHHHHHEECCCHHHHHHHHHCCCCEEEEC
AAFTAFTGKDHWQVLLQARAIENTAYVVAPAQTGRHYGRRQSHGHAMVIDPWGTVLADAG
CHHHHCCCCHHHHHHHHHHHCCCCEEEEEECHHCHHHCCCCCCCCEEEECCCHHHHHCCC
VVQGAAIAPADKERVERIRGQMPSLKHRKTELFI
HHCCCEECCCCHHHHHHHHCCCCCCHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]