Click here to switch to the map view.

The map label for this gene is menD

Identifier: 124025374

GI number: 124025374

Start: 608203

End: 609999

Strand: Direct

Name: menD

Synonym: NATL1_06631

Alternate gene names: 124025374

Gene position: 608203-609999 (Clockwise)

Preceding gene: 124025371

Following gene: 124025375

Centisome position: 32.62

GC content: 35.89

Gene sequence:

>1797_bases
TTGACAATTTCACTAGCTCGTTACAATTTTTTTATCAGCTTAGAGCTCCTTAAAACTCTAGTAGCTAAGGGAGTGAAATA
TTTTGTCCTTTGCCCCGGTAGCAGATCAGGCCCTCTAGCTTTAGCAGCAGCAAGTCTCTCTAAAAGAAAAGAATTAACTC
TCATAACATCCATTGACGAAAGGTCGGCTGCATTCCTTGCTTTGGGAATAAGTGCAGCTAGTGGGCAAGTAAGTTGTGTC
ATTACAACTTCTGGAAGTGCTGTCGCAAATCTTTTGCCGGCTGCTGTTGAAGCAGATAGGTCATGCCATCCTCTCTTGTT
TTTAACTGCAGATAGACCTTTGCGATTAAAAGAATGTGGAGCCAATCAAGCAGTTAATCAACAGGATTTTCTTAAGTCTG
TTTGTAGACATTTTGATGAATCTCCAAAAGAAGGAATTCATTTGATTTCTAAAGAGAGACTGACATCTTTAGTTGGAAAA
TCATTCGAAATGGCTTCTAATATCCCTGGACCAGTTCATATTAACCTTGCTTATGAAGAGCCTTTGCATCCTTGCGAAAT
TGACCAGAATAAAGTTCTTGATGGATGGGGCATTGAAGGATTTTTAAAGGAAAAAGTCACTCCAAATAAGGTTGAGGTTT
TTAAAAGTTTTCAATCTTTAAAACTACCAAAATTAGATCCATTTTCTTTGGGAATAATTATTCTTGGGCCATGGAGAGGA
AAAGTAAAACAACTTAATTCTTTTAGGGGCGCATTAAAGCAATGGCAAAAATTAACTGGATGGCCAATTCTTGCGGATCC
ACTCTCTGGGGTTGAAAATGATCAAGAGGGTTTAATAAATCACTGGGATCTTTTTTTCTCAATAGGTTTATTTGAAAAAA
TCAAAGAGATTCAAGTTTTACGATTGGGTCCAATACCTCCGAGTAGAGAATTACAAACTTGGCTTAAAAAACCTGGGAAA
TTTCAATTACTTATTACTGAGGGAGATTGTCGAAATCTTGATCCCATAGGTGGCTCGACACAATTTAGTGAAGGATTTTC
TTGTTGGGTTGATAAAATGCTTGAGTGTATTCCGGTAAAGCCAGCAATAGATAAAAAAATTGTTAGTCAAAAGTTGACTA
AAGAGCTTATAAAATATGATTTATTTATCAATCATTGGCTCGATAAAAGGTTATTCAGAAATGGGTTAATTACTGAACCT
GCTTTAGCTAGATTGCTTCCTCGCTTGTTACCAGATTCTATCCCTGTGATGATTGCTTCAAGTAGTCCCATAAGAGATTG
GTTGAGTTATTCGGGTGAGGGAGCTTTCCTGAGAAGGTGTTTTGGCTTTAGGGGGGCATCAGGAATTGATGGAACACTCT
CAATGGGTATGGGATTATCAATAATTATGGGGAGAATGGTACTGGTTACTGGCGATTTAGCACTTCTTCATGATACAAAT
GGTTGGCTATTTTCAAAGGATAAAAATATTAGTCTAATTGTAATCATGATTGATAATGGTGGAGGGGGTATATTTAATCA
ATTAAATATAGATAGAATTAAAGAAGGCGATTTTGAAGAAATTTTTCTTATGCCTCAGCAAGTTTGTCATCTTACACTTG
CGAAAGCCTATGGATTGAAATATAAGCAAGTTGCTTGCTTGGATGATTTAGAAAAAGCAATTGAATGGAGTTTTTCTTTG
TCAACAAATGTATTAATAAGAGTTTGTACTAACTCTATTGAAGATCATAGATTGAGAGTAAATTTAAGTGATGACCTAAA
AAAAACATTATCTGAGAATTTATCAAGTTTTGACTAG

Upstream 100 bases:

>100_bases
TTTGGTTTTGATTCTTTTTTGATCCAGTAGATGTCATGAATTTTTTTTGTTTTAGTTACTTGTTGGAAAGTTTTTCACAA
AAATCCAAGTAGTTGTTCTT

Downstream 100 bases:

>100_bases
ATTTAATTAAAAAATGACTTTAAAACCTATTACTCCTAACAATATGTCTAGAAGAGTTCTCCCTGGGGAAATTATTACCT
CATGGGTTTCTGTTGGTGAA

Product: menaquinone biosynthesis protein

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MTISLARYNFFISLELLKTLVAKGVKYFVLCPGSRSGPLALAAASLSKRKELTLITSIDERSAAFLALGISAASGQVSCV
ITTSGSAVANLLPAAVEADRSCHPLLFLTADRPLRLKECGANQAVNQQDFLKSVCRHFDESPKEGIHLISKERLTSLVGK
SFEMASNIPGPVHINLAYEEPLHPCEIDQNKVLDGWGIEGFLKEKVTPNKVEVFKSFQSLKLPKLDPFSLGIIILGPWRG
KVKQLNSFRGALKQWQKLTGWPILADPLSGVENDQEGLINHWDLFFSIGLFEKIKEIQVLRLGPIPPSRELQTWLKKPGK
FQLLITEGDCRNLDPIGGSTQFSEGFSCWVDKMLECIPVKPAIDKKIVSQKLTKELIKYDLFINHWLDKRLFRNGLITEP
ALARLLPRLLPDSIPVMIASSSPIRDWLSYSGEGAFLRRCFGFRGASGIDGTLSMGMGLSIIMGRMVLVTGDLALLHDTN
GWLFSKDKNISLIVIMIDNGGGGIFNQLNIDRIKEGDFEEIFLMPQQVCHLTLAKAYGLKYKQVACLDDLEKAIEWSFSL
STNVLIRVCTNSIEDHRLRVNLSDDLKKTLSENLSSFD

Sequences:

>Translated_598_residues
MTISLARYNFFISLELLKTLVAKGVKYFVLCPGSRSGPLALAAASLSKRKELTLITSIDERSAAFLALGISAASGQVSCV
ITTSGSAVANLLPAAVEADRSCHPLLFLTADRPLRLKECGANQAVNQQDFLKSVCRHFDESPKEGIHLISKERLTSLVGK
SFEMASNIPGPVHINLAYEEPLHPCEIDQNKVLDGWGIEGFLKEKVTPNKVEVFKSFQSLKLPKLDPFSLGIIILGPWRG
KVKQLNSFRGALKQWQKLTGWPILADPLSGVENDQEGLINHWDLFFSIGLFEKIKEIQVLRLGPIPPSRELQTWLKKPGK
FQLLITEGDCRNLDPIGGSTQFSEGFSCWVDKMLECIPVKPAIDKKIVSQKLTKELIKYDLFINHWLDKRLFRNGLITEP
ALARLLPRLLPDSIPVMIASSSPIRDWLSYSGEGAFLRRCFGFRGASGIDGTLSMGMGLSIIMGRMVLVTGDLALLHDTN
GWLFSKDKNISLIVIMIDNGGGGIFNQLNIDRIKEGDFEEIFLMPQQVCHLTLAKAYGLKYKQVACLDDLEKAIEWSFSL
STNVLIRVCTNSIEDHRLRVNLSDDLKKTLSENLSSFD
>Mature_597_residues
TISLARYNFFISLELLKTLVAKGVKYFVLCPGSRSGPLALAAASLSKRKELTLITSIDERSAAFLALGISAASGQVSCVI
TTSGSAVANLLPAAVEADRSCHPLLFLTADRPLRLKECGANQAVNQQDFLKSVCRHFDESPKEGIHLISKERLTSLVGKS
FEMASNIPGPVHINLAYEEPLHPCEIDQNKVLDGWGIEGFLKEKVTPNKVEVFKSFQSLKLPKLDPFSLGIIILGPWRGK
VKQLNSFRGALKQWQKLTGWPILADPLSGVENDQEGLINHWDLFFSIGLFEKIKEIQVLRLGPIPPSRELQTWLKKPGKF
QLLITEGDCRNLDPIGGSTQFSEGFSCWVDKMLECIPVKPAIDKKIVSQKLTKELIKYDLFINHWLDKRLFRNGLITEPA
LARLLPRLLPDSIPVMIASSSPIRDWLSYSGEGAFLRRCFGFRGASGIDGTLSMGMGLSIIMGRMVLVTGDLALLHDTNG
WLFSKDKNISLIVIMIDNGGGGIFNQLNIDRIKEGDFEEIFLMPQQVCHLTLAKAYGLKYKQVACLDDLEKAIEWSFSLS
TNVLIRVCTNSIEDHRLRVNLSDDLKKTLSENLSSFD

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=588, Percent_Identity=27.2108843537415, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PROM1 (A2C165)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014490.1
- ProteinModelPortal:   A2C165
- STRING:   A2C165
- GeneID:   4780405
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_06631
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   TISLARY
- ProtClustDB:   CLSK938786
- BioCyc:   PMAR167555:NATL1_06631-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 66443; Mature: 66312

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISLARYNFFISLELLKTLVAKGVKYFVLCPGSRSGPLALAAASLSKRKELTLITSIDE
CEEEEEEEEEEEEHHHHHHHHHCCCEEEEECCCCCCCCCEEEEHHHHCCCEEEEEEECCC
RSAAFLALGISAASGQVSCVITTSGSAVANLLPAAVEADRSCHPLLFLTADRPLRLKECG
CCCEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCC
ANQAVNQQDFLKSVCRHFDESPKEGIHLISKERLTSLVGKSFEMASNIPGPVHINLAYEE
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEECC
PLHPCEIDQNKVLDGWGIEGFLKEKVTPNKVEVFKSFQSLKLPKLDPFSLGIIILGPWRG
CCCCCCCCCCCEECCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCC
KVKQLNSFRGALKQWQKLTGWPILADPLSGVENDQEGLINHWDLFFSIGLFEKIKEIQVL
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
RLGPIPPSRELQTWLKKPGKFQLLITEGDCRNLDPIGGSTQFSEGFSCWVDKMLECIPVK
EECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PAIDKKIVSQKLTKELIKYDLFINHWLDKRLFRNGLITEPALARLLPRLLPDSIPVMIAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEC
SSPIRDWLSYSGEGAFLRRCFGFRGASGIDGTLSMGMGLSIIMGRMVLVTGDLALLHDTN
CCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHCCCHHHHHCCCEEEECCEEEEECCC
GWLFSKDKNISLIVIMIDNGGGGIFNQLNIDRIKEGDFEEIFLMPQQVCHLTLAKAYGLK
CEEEECCCCCEEEEEEEECCCCCCCCCCCHHHCCCCCHHHHHHCHHHHHHHHHHHHHCCC
YKQVACLDDLEKAIEWSFSLSTNVLIRVCTNSIEDHRLRVNLSDDLKKTLSENLSSFD
HHHHHHHHHHHHHHHCCCCCCCHHHHEEHHCCCCCCEEEEECHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TISLARYNFFISLELLKTLVAKGVKYFVLCPGSRSGPLALAAASLSKRKELTLITSIDE
EEEEEEEEEEEEHHHHHHHHHCCCEEEEECCCCCCCCCEEEEHHHHCCCEEEEEEECCC
RSAAFLALGISAASGQVSCVITTSGSAVANLLPAAVEADRSCHPLLFLTADRPLRLKECG
CCCEEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCC
ANQAVNQQDFLKSVCRHFDESPKEGIHLISKERLTSLVGKSFEMASNIPGPVHINLAYEE
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEECC
PLHPCEIDQNKVLDGWGIEGFLKEKVTPNKVEVFKSFQSLKLPKLDPFSLGIIILGPWRG
CCCCCCCCCCCEECCCCCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCC
KVKQLNSFRGALKQWQKLTGWPILADPLSGVENDQEGLINHWDLFFSIGLFEKIKEIQVL
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEE
RLGPIPPSRELQTWLKKPGKFQLLITEGDCRNLDPIGGSTQFSEGFSCWVDKMLECIPVK
EECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
PAIDKKIVSQKLTKELIKYDLFINHWLDKRLFRNGLITEPALARLLPRLLPDSIPVMIAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEC
SSPIRDWLSYSGEGAFLRRCFGFRGASGIDGTLSMGMGLSIIMGRMVLVTGDLALLHDTN
CCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHCCCHHHHHCCCEEEECCEEEEECCC
GWLFSKDKNISLIVIMIDNGGGGIFNQLNIDRIKEGDFEEIFLMPQQVCHLTLAKAYGLK
CEEEECCCCCEEEEEEEECCCCCCCCCCCHHHCCCCCHHHHHHCHHHHHHHHHHHHHCCC
YKQVACLDDLEKAIEWSFSLSTNVLIRVCTNSIEDHRLRVNLSDDLKKTLSENLSSFD
HHHHHHHHHHHHHHHCCCCCCCHHHHEEHHCCCCCCEEEEECHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA