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The map label for this gene is clpB [H]

Identifier: 124025347

GI number: 124025347

Start: 575221

End: 577812

Strand: Reverse

Name: clpB [H]

Synonym: NATL1_06361

Alternate gene names: 124025347

Gene position: 577812-575221 (Counterclockwise)

Preceding gene: 124025348

Following gene: 124025345

Centisome position: 30.99

GC content: 34.65

Gene sequence:

>2592_bases
ATGAAGGTAAATCCTGATGATTTCACTAACATTGCTTGGCAAGGGATTATTGATGCAAAAGATTTAGCATTAACTGAAAA
ACATCAGACTTTAGAGACAGAACATCTTTTTTGGTCCCTTCTAAAGAAAAATGAAATAGCAATAAAAATAATAGAAAGAT
CAGGCGGAGTAATAAAAAATCTACTCACAGAAATAGAGAATTTCATAAAGAACCAACCAAAAATGCTACAGGCCCAAGAA
TCTATTTTCTTTGGCAAAAATATATCTTTATCAATTTCAAGAGCAAAAAACATTCAACAATCTTTCAAAGATGATTTTAT
TTCAAGCGAACATTTAGTGATTTCCCTATTTGATGACGAGAGAATTTGTAATAGGTTATTTGGACAAAATCATGTCGACA
AAAACAGCCTTCTTGAGGCTATAAATGTTCTTAGAGGTGACAAAAAAGTGACCCAGAAAAATGCTGAGAATAGCTACGAA
GCTCTTCAAAAGTACGGATTAGACCTTACTTCTGCCGCCAGAGATGGAAAATTAGATCCTGTAATTGGAAGAGATGAGGA
GATACGAAGAACGATTCAAATACTAAGCCGCAGAACCAAAAATAATCCTGTGTTAATTGGAGAAGCTGGCGTTGGCAAAA
CAGCAATTATTGAGGGTTTAGCACAAAGAATTATCAACGGTGATGTTCCTACAGCTCTTGAGAACCGTCAACTAATTTCT
TTAGACATGGGGGCACTAATAGCAGGTGCAAAATTTCGTGGTGAATTTGAAGAGAGACTTAAGTCAGTTCTTAAAAATGT
CACAGATTCAGAAGGGAAAATAATTTTGTTCATCGATGAAATACATACTGTCGTCGGAGCAGGAGCGAGTGGTGGAGCTA
TGGACGCTAGCAATCTCCTAAAACCAATGCTTGCTAGAGGTGAACTGAGATGCATTGGAGCAACAACCATAAATGAACAT
AGAGAAAATTTTGAGAAAGACCCTGCGCTGGAAAGAAGATTTCAGCAAATACTTGTAAAACAACCCTCAGTCCAAGATAC
AATTTCGATCTTACGTGGACTAAAAGAAAGATATGAAGTCCATCATGGTGTTCGTATCTCTGATAACGCTCTAATAGCTG
CGGCGATATTGAGCGATAGATATATACCAGAGAGGTTTTTACCTGACAAAGCAATTGATCTAATAGATGAATCAGCCTCA
CGTTTAAAGATGGAAATAACATCAAAACCAGAAGAAATTGACGAGATTGATAGAAAAATCATCCAACTTGAGATGGAGAA
ATTATCTTTAGAAGGTGAGTCAGACACATCAAGTAAAGAGAGATTAGAATTAATTACTACAGAACTAGCATTGCTAACAA
AAAATCAAATTGAAGTAACAAAAAAATGGAAACAAGAAAAAGAATCAATCGAAGAAATTTCAACACTTAAAGAAGACATT
GAGAAAGTACAACTAGAAATAGAAAAAGCAAAAAGAAATTATGACCTAAATAGAGCTGCTGAACTTGAATACGGCACCCT
TGTGAATTATCAACAAAATTTAAAATCTAAAGAACTTAATCTAAAAAATTCCACTGAAAATGCGGAAAAATCACTTTTGA
GAGAAGAGGTTTTAGCCGATGATGTAGCTGAAATAATTGCAAAGTGGACATCTATTCCCGTTAAAAGATTGGCTCAAACT
GAAATTGAAAAATTACTCAATCTTGAATCTCAGCTTCAAAAAAGAGTTATTGGTCAAGATAAAGCCGTTCAATCAATATC
TTCTGCGATACAAAGATCTAGGACAGGACTTAGTGATCCAAGCAGACCAATCGCAAGTTTTTTATTTTTAGGACCGACAG
GCGTGGGGAAGACAGAATTGTCCAAGGCTTTGGCTTCTCAACTTTTTGATAGTGAAAATGCCTTAATCAGAATAGACATG
TCTGAATATATGGAAAAGCACTCTATAAGTCGCTTAATTGGCGCTCCTCCTGGTTACGTTGGTTACGAAGCAGGTGGGCA
ATTAACAGAAGCAATCAGAAGAAAACCTTATTGCGTTTTGCTCTTCGATGAAATTGAAAAAGCACATAAAGATGTTTTCA
ATGTATTGCTTCAGATACTTGACGAAGGCCGAGTAACTGATGGACAAGGTAGAACAACAAATTTCAAAAACACTATTATT
ATTCTCACAAGTAATTTAGGAAGCGAATTAATCTCTGATAATGATGTAACTAATGATCCGTCGACAAATATAGATGAACT
TATTAATCAAGAGCTGAAATCAAATTTCAGGCCTGAGTTCCTAAACAGACTTGATGAAATAATTAATTTTGAACCACTAA
AAAAAGAAACACTACTTAAAGTAGTCGATTTACAATTAAACCGTTTAAGAGAAAGGTTAGAAGCTAAAGGAATCGAGCTT
GAAATTAATGATGATGTCCTATCTTTGATTACTGAACTTGGATACAACCCAAGCTATGGTGCTAGGCCCTTAAAAAGAGT
TATTCAAAAAGAATTAGAAAGTGAAATAGCAAAATATATTCTTAAAGGCAAATATAAAGAAGGAAGCACTATAAAGATTG
AGAGTAAAGAATCAAAATTAATTTTCCACTAA

Upstream 100 bases:

>100_bases
GATTTAACAAATTTGTTTTTCCAAGTCAACAAGGTCGGTTTTCTTATATTTATTCAATCAACAGATTTGAATAGTATATA
TATGGTAATTAATTTAAAGA

Downstream 100 bases:

>100_bases
AAAACTGAAAACGTTAATTGTATTTCAACAGGAAGTCTTACTGTATCAAGGGTTGAACCACTCAGGGAGGAAAGTATAAC
TAGCGGAATTAACAGCATTT

Product: ATP-dependent Clp protease, Hsp 100, ATP-binding subunit ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MKVNPDDFTNIAWQGIIDAKDLALTEKHQTLETEHLFWSLLKKNEIAIKIIERSGGVIKNLLTEIENFIKNQPKMLQAQE
SIFFGKNISLSISRAKNIQQSFKDDFISSEHLVISLFDDERICNRLFGQNHVDKNSLLEAINVLRGDKKVTQKNAENSYE
ALQKYGLDLTSAARDGKLDPVIGRDEEIRRTIQILSRRTKNNPVLIGEAGVGKTAIIEGLAQRIINGDVPTALENRQLIS
LDMGALIAGAKFRGEFEERLKSVLKNVTDSEGKIILFIDEIHTVVGAGASGGAMDASNLLKPMLARGELRCIGATTINEH
RENFEKDPALERRFQQILVKQPSVQDTISILRGLKERYEVHHGVRISDNALIAAAILSDRYIPERFLPDKAIDLIDESAS
RLKMEITSKPEEIDEIDRKIIQLEMEKLSLEGESDTSSKERLELITTELALLTKNQIEVTKKWKQEKESIEEISTLKEDI
EKVQLEIEKAKRNYDLNRAAELEYGTLVNYQQNLKSKELNLKNSTENAEKSLLREEVLADDVAEIIAKWTSIPVKRLAQT
EIEKLLNLESQLQKRVIGQDKAVQSISSAIQRSRTGLSDPSRPIASFLFLGPTGVGKTELSKALASQLFDSENALIRIDM
SEYMEKHSISRLIGAPPGYVGYEAGGQLTEAIRRKPYCVLLFDEIEKAHKDVFNVLLQILDEGRVTDGQGRTTNFKNTII
ILTSNLGSELISDNDVTNDPSTNIDELINQELKSNFRPEFLNRLDEIINFEPLKKETLLKVVDLQLNRLRERLEAKGIEL
EINDDVLSLITELGYNPSYGARPLKRVIQKELESEIAKYILKGKYKEGSTIKIESKESKLIFH

Sequences:

>Translated_863_residues
MKVNPDDFTNIAWQGIIDAKDLALTEKHQTLETEHLFWSLLKKNEIAIKIIERSGGVIKNLLTEIENFIKNQPKMLQAQE
SIFFGKNISLSISRAKNIQQSFKDDFISSEHLVISLFDDERICNRLFGQNHVDKNSLLEAINVLRGDKKVTQKNAENSYE
ALQKYGLDLTSAARDGKLDPVIGRDEEIRRTIQILSRRTKNNPVLIGEAGVGKTAIIEGLAQRIINGDVPTALENRQLIS
LDMGALIAGAKFRGEFEERLKSVLKNVTDSEGKIILFIDEIHTVVGAGASGGAMDASNLLKPMLARGELRCIGATTINEH
RENFEKDPALERRFQQILVKQPSVQDTISILRGLKERYEVHHGVRISDNALIAAAILSDRYIPERFLPDKAIDLIDESAS
RLKMEITSKPEEIDEIDRKIIQLEMEKLSLEGESDTSSKERLELITTELALLTKNQIEVTKKWKQEKESIEEISTLKEDI
EKVQLEIEKAKRNYDLNRAAELEYGTLVNYQQNLKSKELNLKNSTENAEKSLLREEVLADDVAEIIAKWTSIPVKRLAQT
EIEKLLNLESQLQKRVIGQDKAVQSISSAIQRSRTGLSDPSRPIASFLFLGPTGVGKTELSKALASQLFDSENALIRIDM
SEYMEKHSISRLIGAPPGYVGYEAGGQLTEAIRRKPYCVLLFDEIEKAHKDVFNVLLQILDEGRVTDGQGRTTNFKNTII
ILTSNLGSELISDNDVTNDPSTNIDELINQELKSNFRPEFLNRLDEIINFEPLKKETLLKVVDLQLNRLRERLEAKGIEL
EINDDVLSLITELGYNPSYGARPLKRVIQKELESEIAKYILKGKYKEGSTIKIESKESKLIFH
>Mature_863_residues
MKVNPDDFTNIAWQGIIDAKDLALTEKHQTLETEHLFWSLLKKNEIAIKIIERSGGVIKNLLTEIENFIKNQPKMLQAQE
SIFFGKNISLSISRAKNIQQSFKDDFISSEHLVISLFDDERICNRLFGQNHVDKNSLLEAINVLRGDKKVTQKNAENSYE
ALQKYGLDLTSAARDGKLDPVIGRDEEIRRTIQILSRRTKNNPVLIGEAGVGKTAIIEGLAQRIINGDVPTALENRQLIS
LDMGALIAGAKFRGEFEERLKSVLKNVTDSEGKIILFIDEIHTVVGAGASGGAMDASNLLKPMLARGELRCIGATTINEH
RENFEKDPALERRFQQILVKQPSVQDTISILRGLKERYEVHHGVRISDNALIAAAILSDRYIPERFLPDKAIDLIDESAS
RLKMEITSKPEEIDEIDRKIIQLEMEKLSLEGESDTSSKERLELITTELALLTKNQIEVTKKWKQEKESIEEISTLKEDI
EKVQLEIEKAKRNYDLNRAAELEYGTLVNYQQNLKSKELNLKNSTENAEKSLLREEVLADDVAEIIAKWTSIPVKRLAQT
EIEKLLNLESQLQKRVIGQDKAVQSISSAIQRSRTGLSDPSRPIASFLFLGPTGVGKTELSKALASQLFDSENALIRIDM
SEYMEKHSISRLIGAPPGYVGYEAGGQLTEAIRRKPYCVLLFDEIEKAHKDVFNVLLQILDEGRVTDGQGRTTNFKNTII
ILTSNLGSELISDNDVTNDPSTNIDELINQELKSNFRPEFLNRLDEIINFEPLKKETLLKVVDLQLNRLRERLEAKGIEL
EINDDVLSLITELGYNPSYGARPLKRVIQKELESEIAKYILKGKYKEGSTIKIESKESKLIFH

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=320, Percent_Identity=35.9375, Blast_Score=211, Evalue=3e-54,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=51.5752625437573, Blast_Score=901, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=408, Percent_Identity=40.4411764705882, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=712, Percent_Identity=50.8426966292135, Blast_Score=741, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=869, Percent_Identity=41.542002301496, Blast_Score=655, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 97443; Mature: 97443

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNPDDFTNIAWQGIIDAKDLALTEKHQTLETEHLFWSLLKKNEIAIKIIERSGGVIKN
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
LLTEIENFIKNQPKMLQAQESIFFGKNISLSISRAKNIQQSFKDDFISSEHLVISLFDDE
HHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCH
RICNRLFGQNHVDKNSLLEAINVLRGDKKVTQKNAENSYEALQKYGLDLTSAARDGKLDP
HHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCC
VIGRDEEIRRTIQILSRRTKNNPVLIGEAGVGKTAIIEGLAQRIINGDVPTALENRQLIS
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LDMGALIAGAKFRGEFEERLKSVLKNVTDSEGKIILFIDEIHTVVGAGASGGAMDASNLL
ECHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHCCCCCCCCCCHHHHH
KPMLARGELRCIGATTINEHRENFEKDPALERRFQQILVKQPSVQDTISILRGLKERYEV
HHHHHCCCEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HHGVRISDNALIAAAILSDRYIPERFLPDKAIDLIDESASRLKMEITSKPEEIDEIDRKI
HCCEEECCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
IQLEMEKLSLEGESDTSSKERLELITTELALLTKNQIEVTKKWKQEKESIEEISTLKEDI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EKVQLEIEKAKRNYDLNRAAELEYGTLVNYQQNLKSKELNLKNSTENAEKSLLREEVLAD
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHH
DVAEIIAKWTSIPVKRLAQTEIEKLLNLESQLQKRVIGQDKAVQSISSAIQRSRTGLSDP
HHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
SRPIASFLFLGPTGVGKTELSKALASQLFDSENALIRIDMSEYMEKHSISRLIGAPPGYV
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCC
GYEAGGQLTEAIRRKPYCVLLFDEIEKAHKDVFNVLLQILDEGRVTDGQGRTTNFKNTII
CCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
ILTSNLGSELISDNDVTNDPSTNIDELINQELKSNFRPEFLNRLDEIINFEPLKKETLLK
EEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHH
VVDLQLNRLRERLEAKGIELEINDDVLSLITELGYNPSYGARPLKRVIQKELESEIAKYI
HHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKGKYKEGSTIKIESKESKLIFH
HHCCCCCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKVNPDDFTNIAWQGIIDAKDLALTEKHQTLETEHLFWSLLKKNEIAIKIIERSGGVIKN
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
LLTEIENFIKNQPKMLQAQESIFFGKNISLSISRAKNIQQSFKDDFISSEHLVISLFDDE
HHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCH
RICNRLFGQNHVDKNSLLEAINVLRGDKKVTQKNAENSYEALQKYGLDLTSAARDGKLDP
HHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCCC
VIGRDEEIRRTIQILSRRTKNNPVLIGEAGVGKTAIIEGLAQRIINGDVPTALENRQLIS
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEE
LDMGALIAGAKFRGEFEERLKSVLKNVTDSEGKIILFIDEIHTVVGAGASGGAMDASNLL
ECHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHCCCCCCCCCCHHHHH
KPMLARGELRCIGATTINEHRENFEKDPALERRFQQILVKQPSVQDTISILRGLKERYEV
HHHHHCCCEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
HHGVRISDNALIAAAILSDRYIPERFLPDKAIDLIDESASRLKMEITSKPEEIDEIDRKI
HCCEEECCCCHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHH
IQLEMEKLSLEGESDTSSKERLELITTELALLTKNQIEVTKKWKQEKESIEEISTLKEDI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EKVQLEIEKAKRNYDLNRAAELEYGTLVNYQQNLKSKELNLKNSTENAEKSLLREEVLAD
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHH
DVAEIIAKWTSIPVKRLAQTEIEKLLNLESQLQKRVIGQDKAVQSISSAIQRSRTGLSDP
HHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
SRPIASFLFLGPTGVGKTELSKALASQLFDSENALIRIDMSEYMEKHSISRLIGAPPGYV
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCC
GYEAGGQLTEAIRRKPYCVLLFDEIEKAHKDVFNVLLQILDEGRVTDGQGRTTNFKNTII
CCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
ILTSNLGSELISDNDVTNDPSTNIDELINQELKSNFRPEFLNRLDEIINFEPLKKETLLK
EEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHH
VVDLQLNRLRERLEAKGIELEINDDVLSLITELGYNPSYGARPLKRVIQKELESEIAKYI
HHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LKGKYKEGSTIKIESKESKLIFH
HHCCCCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]