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The map label for this gene is gloB

Identifier: 124025325

GI number: 124025325

Start: 557417

End: 558172

Strand: Reverse

Name: gloB

Synonym: NATL1_06141

Alternate gene names: 124025325

Gene position: 558172-557417 (Counterclockwise)

Preceding gene: 124025329

Following gene: 124025324

Centisome position: 29.93

GC content: 34.39

Gene sequence:

>756_bases
ATGTTAGGGGAAAAAAGCGACTTCACTATTCACCCAATTAATGTCTTACAGGACAATATCGTATGGGTATGGGTCCACAA
TTGCAATGCAGTGGTAGTAGATCCCTCCATATCAGGGCCAGTAGAAAAATGGCTTTTAGAAAAGAACCTATCATTAAAAG
CTATCCTTCAAACACATCATCATGATGACCATATTGGTGGAACACAAAAGTTAATCAAAACATGGCCAGAGGCAAAGGTA
GTTGCATCAAAAAAAGAACATAAAAGAATACCTTTTCAAACATTTTCGGTTGACGATAATGATATTTTTAATTTAATGGA
TGCTGAGATTAAAGTTATCGAAGTGCATGGTCACACTGACAACCATATAGCTTTTTACATATCAAAACAAAATGCTAAAT
GTAATATACTATTCCCAGGAGATACGCTATTTGGAGGGGGTTGTGGTCGTCTACTAGAAGGTAGTCCTGTTCAAATGTTT
GAAAGTTTATATAAACTTAATTCTCTTCCAGAAAACACTGAAATATACCCAGCTCATGAATACACAGAAAGCAACTTAAA
ATGGGCCCTATCATTAGAGCCTGGAAATATTTCTATCATCGAAAGACTAAAGCTTATTCAAAAAAAACTTCAAAAGGGAA
TGTCTAGTCTCCCATCAACACTCTCAGAAGAAAGAAAAACAAATTTATTTTTAATAGCAGAAAATGTCGAGAAATTTACA
ATGCTAAGGAAACACAAAGATAGATGGAAATGTTAA

Upstream 100 bases:

>100_bases
GAGACTGATTCTTGCAATAAGGCACCTTTAGCTAATGCAACAGTAAACATGTCAAAAACTCTTGAAATGGACTCTAATTA
CTCCATTACCTAAAGAAACA

Downstream 100 bases:

>100_bases
ATTCTAATTTGTGTATCAAATCTATTCATTAAAGTCTAATCATTCATGAAAAGTTCATCGATTGAGGCCATAGAAACAAA
ATTTGCGCCTAAGCCCGTTG

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLGEKSDFTIHPINVLQDNIVWVWVHNCNAVVVDPSISGPVEKWLLEKNLSLKAILQTHHHDDHIGGTQKLIKTWPEAKV
VASKKEHKRIPFQTFSVDDNDIFNLMDAEIKVIEVHGHTDNHIAFYISKQNAKCNILFPGDTLFGGGCGRLLEGSPVQMF
ESLYKLNSLPENTEIYPAHEYTESNLKWALSLEPGNISIIERLKLIQKKLQKGMSSLPSTLSEERKTNLFLIAENVEKFT
MLRKHKDRWKC

Sequences:

>Translated_251_residues
MLGEKSDFTIHPINVLQDNIVWVWVHNCNAVVVDPSISGPVEKWLLEKNLSLKAILQTHHHDDHIGGTQKLIKTWPEAKV
VASKKEHKRIPFQTFSVDDNDIFNLMDAEIKVIEVHGHTDNHIAFYISKQNAKCNILFPGDTLFGGGCGRLLEGSPVQMF
ESLYKLNSLPENTEIYPAHEYTESNLKWALSLEPGNISIIERLKLIQKKLQKGMSSLPSTLSEERKTNLFLIAENVEKFT
MLRKHKDRWKC
>Mature_251_residues
MLGEKSDFTIHPINVLQDNIVWVWVHNCNAVVVDPSISGPVEKWLLEKNLSLKAILQTHHHDDHIGGTQKLIKTWPEAKV
VASKKEHKRIPFQTFSVDDNDIFNLMDAEIKVIEVHGHTDNHIAFYISKQNAKCNILFPGDTLFGGGCGRLLEGSPVQMF
ESLYKLNSLPENTEIYPAHEYTESNLKWALSLEPGNISIIERLKLIQKKLQKGMSSLPSTLSEERKTNLFLIAENVEKFT
MLRKHKDRWKC

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI116642887, Length=223, Percent_Identity=34.0807174887892, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI21703352, Length=223, Percent_Identity=34.0807174887892, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI94538320, Length=254, Percent_Identity=31.8897637795276, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=30.7984790874525, Blast_Score=126, Evalue=1e-29,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=33.0472103004292, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI46361987, Length=200, Percent_Identity=30.5, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786406, Length=257, Percent_Identity=36.1867704280156, Blast_Score=140, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17536925, Length=189, Percent_Identity=36.5079365079365, Blast_Score=137, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17538952, Length=169, Percent_Identity=28.9940828402367, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320478, Length=219, Percent_Identity=31.5068493150685, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324614, Length=175, Percent_Identity=31.4285714285714, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24667711, Length=253, Percent_Identity=34.7826086956522, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21356335, Length=254, Percent_Identity=34.6456692913386, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24667703, Length=256, Percent_Identity=34.375, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI221330176, Length=171, Percent_Identity=32.7485380116959, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_PROM1 (A2C116)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014441.1
- ProteinModelPortal:   A2C116
- SMR:   A2C116
- STRING:   A2C116
- GeneID:   4779603
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_06141
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK716142
- BioCyc:   PMAR167555:NATL1_06141-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28692; Mature: 28692

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGEKSDFTIHPINVLQDNIVWVWVHNCNAVVVDPSISGPVEKWLLEKNLSLKAILQTHH
CCCCCCCCEEEEHHHCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHCC
HDDHIGGTQKLIKTWPEAKVVASKKEHKRIPFQTFSVDDNDIFNLMDAEIKVIEVHGHTD
CCCCCCHHHHHHHHCCCHHEECCCHHHCCCCEEEECCCCCHHHEEECCEEEEEEEECCCC
NHIAFYISKQNAKCNILFPGDTLFGGGCGRLLEGSPVQMFESLYKLNSLPENTEIYPAHE
CEEEEEEECCCCEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCC
YTESNLKWALSLEPGNISIIERLKLIQKKLQKGMSSLPSTLSEERKTNLFLIAENVEKFT
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHH
MLRKHKDRWKC
HHHHHHHHCCC
>Mature Secondary Structure
MLGEKSDFTIHPINVLQDNIVWVWVHNCNAVVVDPSISGPVEKWLLEKNLSLKAILQTHH
CCCCCCCCEEEEHHHCCCCEEEEEEECCCEEEECCCCCCHHHHHHHHCCCCCEEHHHHCC
HDDHIGGTQKLIKTWPEAKVVASKKEHKRIPFQTFSVDDNDIFNLMDAEIKVIEVHGHTD
CCCCCCHHHHHHHHCCCHHEECCCHHHCCCCEEEECCCCCHHHEEECCEEEEEEEECCCC
NHIAFYISKQNAKCNILFPGDTLFGGGCGRLLEGSPVQMFESLYKLNSLPENTEIYPAHE
CEEEEEEECCCCEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCC
YTESNLKWALSLEPGNISIIERLKLIQKKLQKGMSSLPSTLSEERKTNLFLIAENVEKFT
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHH
MLRKHKDRWKC
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA