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The map label for this gene is proS

Identifier: 124025277

GI number: 124025277

Start: 512261

End: 514051

Strand: Reverse

Name: proS

Synonym: NATL1_05661

Alternate gene names: 124025277

Gene position: 514051-512261 (Counterclockwise)

Preceding gene: 124025283

Following gene: 124025276

Centisome position: 27.57

GC content: 33.22

Gene sequence:

>1791_bases
ATGCGCGTCTCCCGCCTAATGCTGAACACTCTTAGAGACGTCCCTTCAGAAGCAGATATAATTTCACATCAGTTACTGGT
AAGAGGTGGTTATATTAAGCGCATAACCGGAGGTATTTATGCATATATGCCATTACTTTGGAAGGTTCTAAAAAAAATTA
CCTCAATAGTTGAAGAAGAGTTATCAACAAAAGGTTGCCTGCAAACTCTTCTCCCTCAACTTCAGCCTTCAGAAATATGG
GAAAGAAGTGGGAGGTGGAAATCATATACACAGGGAGAAGGTATTATGTTTAGTCTTAAAGATAGACAAGGGAAAGAACT
AGGACTGGGACCAACGCATGAAGAAGTAATTACGCAAATAATTTCTCAAACTATTCACTCTTACAAACAATTACCGATAA
ATATATTCCAAATTCAAACAAAATTTAGAGATGAAATAAGACCAAGATTTGGGTTAATGAGAAGTAGAGAATTCATCATG
AAGGATGCTTATTCCTTTCATGCAAATGAAAATGATCTTCAATCAACTTATTCAGACATGAGAAATGCCTATCAAAATAT
ATTTACAAAATGTGGTCTAGATTTTGTTTGTGTCGACGCAGATAGTGGAGCAATTGGGGGTGCAGCATCTCAAGAATTCA
TGGTAACAGCTGAGTCTGGGGAGGACTTAATTTTGATAAGTTCTGATGGCAAGTATGGGGCTAATCAAGAAAAAGCTGTT
TCCATTATTGAAGAAGGAAACTTATTAGAACCTAATAAACCATCGATAATTAAGACTCCTAATCAAAAAACAATAGATGA
ATTATGTAATTACAATGATTTCCACCCAAGTCAAATTGTAAAAGTATTAGCTTATCTAGCAACGTGTGATGATAATAAAA
AATACCCAGTTCTAGTAAGTATTCGGGGGGATCAAGAAATAAATGATATTAAACTTTCAAATAAAATATCTCAAGAATTA
AAGAAAAATGTACTTGATATTAGAATTATTTATAATGAAGACATGCAAAAGCAAGGCATTACTAATATACCATTTGGTTT
TATAGGTCCTGATCTTAGCGATAATTTACTTGCACAATCAAAAGGATGGGAAAAAAAATTCATAAGAATCGCTGACAATT
CTGCAAAAGATCTTAAAAGTTTTATATGTGGAAACAATATTAAAGATGAGCATAAAATATTTTATAATTGGAATCTAATT
AATACTGTGCAACTGATATGTGATATTAGAAAAGCCAAACCAGGAGACAGGTGTATTCATGATAAAACACAAAAACTTGA
AGAATGTAGAGGGATAGAAATAGGGCATATATTTCAATTAGGAACTAAGTATTCTAAATCATTAAATGCTACTTTTACCA
ACGAAAAAGGTATTGAAGACCACTTGTGGATGGGGTGCTATGGAATTGGTATTTCCAGATTAGCTCAAGCAGCAGTAGAA
CAAAATCATGATGATTTAGGTATTATCTGGCCGACATCAATTGCCCCTTTTACAGTAATAATTATCATTGCCAATATAAA
GAATAATGATCAAAAATGTTTAGCTGAAGATATCTATCAAAAATTAATACAAAATCGAGTTGATGTTCTTCTTGACGATA
GGGATGATAGGGCTGGGATCAAGTTTAAAGATGCAGACCTTATTGGAATCCCATGGAGGATTGTTGCTGGGCGAGAAGCT
AGTTCGGGACTAGTTGAATTACATAATAGAAAAACAAAAACTACAGAGTTGTTAGATCTGAACTCCGTTTTAAAAAAGCT
TTCTGAAGAATTTAATACTGAAAAACTATAA

Upstream 100 bases:

>100_bases
ACACTTTAAAAAAACAACATGATTCCCGCACAAACAATATAGATATTCTTATGGCCAACACTCAAAGGACTAATCTCATA
AAGATTTAAATTTTAGGCTT

Downstream 100 bases:

>100_bases
ATTGAGCCCAAAGGACTCTAGAGTCTTACGTAACTGCAAAAAACAAATGATTTCTGCCTTTCATCGCCTCTCCATCAGGT
TGGTGAGGGCAGCTTTGGCT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MRVSRLMLNTLRDVPSEADIISHQLLVRGGYIKRITGGIYAYMPLLWKVLKKITSIVEEELSTKGCLQTLLPQLQPSEIW
ERSGRWKSYTQGEGIMFSLKDRQGKELGLGPTHEEVITQIISQTIHSYKQLPINIFQIQTKFRDEIRPRFGLMRSREFIM
KDAYSFHANENDLQSTYSDMRNAYQNIFTKCGLDFVCVDADSGAIGGAASQEFMVTAESGEDLILISSDGKYGANQEKAV
SIIEEGNLLEPNKPSIIKTPNQKTIDELCNYNDFHPSQIVKVLAYLATCDDNKKYPVLVSIRGDQEINDIKLSNKISQEL
KKNVLDIRIIYNEDMQKQGITNIPFGFIGPDLSDNLLAQSKGWEKKFIRIADNSAKDLKSFICGNNIKDEHKIFYNWNLI
NTVQLICDIRKAKPGDRCIHDKTQKLEECRGIEIGHIFQLGTKYSKSLNATFTNEKGIEDHLWMGCYGIGISRLAQAAVE
QNHDDLGIIWPTSIAPFTVIIIIANIKNNDQKCLAEDIYQKLIQNRVDVLLDDRDDRAGIKFKDADLIGIPWRIVAGREA
SSGLVELHNRKTKTTELLDLNSVLKKLSEEFNTEKL

Sequences:

>Translated_596_residues
MRVSRLMLNTLRDVPSEADIISHQLLVRGGYIKRITGGIYAYMPLLWKVLKKITSIVEEELSTKGCLQTLLPQLQPSEIW
ERSGRWKSYTQGEGIMFSLKDRQGKELGLGPTHEEVITQIISQTIHSYKQLPINIFQIQTKFRDEIRPRFGLMRSREFIM
KDAYSFHANENDLQSTYSDMRNAYQNIFTKCGLDFVCVDADSGAIGGAASQEFMVTAESGEDLILISSDGKYGANQEKAV
SIIEEGNLLEPNKPSIIKTPNQKTIDELCNYNDFHPSQIVKVLAYLATCDDNKKYPVLVSIRGDQEINDIKLSNKISQEL
KKNVLDIRIIYNEDMQKQGITNIPFGFIGPDLSDNLLAQSKGWEKKFIRIADNSAKDLKSFICGNNIKDEHKIFYNWNLI
NTVQLICDIRKAKPGDRCIHDKTQKLEECRGIEIGHIFQLGTKYSKSLNATFTNEKGIEDHLWMGCYGIGISRLAQAAVE
QNHDDLGIIWPTSIAPFTVIIIIANIKNNDQKCLAEDIYQKLIQNRVDVLLDDRDDRAGIKFKDADLIGIPWRIVAGREA
SSGLVELHNRKTKTTELLDLNSVLKKLSEEFNTEKL
>Mature_596_residues
MRVSRLMLNTLRDVPSEADIISHQLLVRGGYIKRITGGIYAYMPLLWKVLKKITSIVEEELSTKGCLQTLLPQLQPSEIW
ERSGRWKSYTQGEGIMFSLKDRQGKELGLGPTHEEVITQIISQTIHSYKQLPINIFQIQTKFRDEIRPRFGLMRSREFIM
KDAYSFHANENDLQSTYSDMRNAYQNIFTKCGLDFVCVDADSGAIGGAASQEFMVTAESGEDLILISSDGKYGANQEKAV
SIIEEGNLLEPNKPSIIKTPNQKTIDELCNYNDFHPSQIVKVLAYLATCDDNKKYPVLVSIRGDQEINDIKLSNKISQEL
KKNVLDIRIIYNEDMQKQGITNIPFGFIGPDLSDNLLAQSKGWEKKFIRIADNSAKDLKSFICGNNIKDEHKIFYNWNLI
NTVQLICDIRKAKPGDRCIHDKTQKLEECRGIEIGHIFQLGTKYSKSLNATFTNEKGIEDHLWMGCYGIGISRLAQAAVE
QNHDDLGIIWPTSIAPFTVIIIIANIKNNDQKCLAEDIYQKLIQNRVDVLLDDRDDRAGIKFKDADLIGIPWRIVAGREA
SSGLVELHNRKTKTTELLDLNSVLKKLSEEFNTEKL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=40.6113537117904, Blast_Score=182, Evalue=9e-46,
Organism=Escherichia coli, GI1786392, Length=595, Percent_Identity=40.1680672268908, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI115532348, Length=209, Percent_Identity=36.8421052631579, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320931, Length=205, Percent_Identity=38.0487804878049, Blast_Score=156, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24656200, Length=217, Percent_Identity=36.405529953917, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PROM1 (A2C0W8)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014393.1
- STRING:   A2C0W8
- GeneID:   4780178
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_05661
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   PMAR167555:NATL1_05661-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 67643; Mature: 67643

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSRLMLNTLRDVPSEADIISHQLLVRGGYIKRITGGIYAYMPLLWKVLKKITSIVEEE
CCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSTKGCLQTLLPQLQPSEIWERSGRWKSYTQGEGIMFSLKDRQGKELGLGPTHEEVITQI
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
ISQTIHSYKQLPINIFQIQTKFRDEIRPRFGLMRSREFIMKDAYSFHANENDLQSTYSDM
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
RNAYQNIFTKCGLDFVCVDADSGAIGGAASQEFMVTAESGEDLILISSDGKYGANQEKAV
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHH
SIIEEGNLLEPNKPSIIKTPNQKTIDELCNYNDFHPSQIVKVLAYLATCDDNKKYPVLVS
HHHHCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IRGDQEINDIKLSNKISQELKKNVLDIRIIYNEDMQKQGITNIPFGFIGPDLSDNLLAQS
ECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHCCCCCCCCCCCCCCCCCHHHHHC
KGWEKKFIRIADNSAKDLKSFICGNNIKDEHKIFYNWNLINTVQLICDIRKAKPGDRCIH
CCCCCCEEEECCCCHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHH
DKTQKLEECRGIEIGHIFQLGTKYSKSLNATFTNEKGIEDHLWMGCYGIGISRLAQAAVE
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
QNHDDLGIIWPTSIAPFTVIIIIANIKNNDQKCLAEDIYQKLIQNRVDVLLDDRDDRAGI
CCCCCEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
KFKDADLIGIPWRIVAGREASSGLVELHNRKTKTTELLDLNSVLKKLSEEFNTEKL
EECCCCEECCCEEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRVSRLMLNTLRDVPSEADIISHQLLVRGGYIKRITGGIYAYMPLLWKVLKKITSIVEEE
CCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSTKGCLQTLLPQLQPSEIWERSGRWKSYTQGEGIMFSLKDRQGKELGLGPTHEEVITQI
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
ISQTIHSYKQLPINIFQIQTKFRDEIRPRFGLMRSREFIMKDAYSFHANENDLQSTYSDM
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
RNAYQNIFTKCGLDFVCVDADSGAIGGAASQEFMVTAESGEDLILISSDGKYGANQEKAV
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHH
SIIEEGNLLEPNKPSIIKTPNQKTIDELCNYNDFHPSQIVKVLAYLATCDDNKKYPVLVS
HHHHCCCCCCCCCCCEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
IRGDQEINDIKLSNKISQELKKNVLDIRIIYNEDMQKQGITNIPFGFIGPDLSDNLLAQS
ECCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCHHHCCCCCCCCCCCCCCCCCHHHHHC
KGWEKKFIRIADNSAKDLKSFICGNNIKDEHKIFYNWNLINTVQLICDIRKAKPGDRCIH
CCCCCCEEEECCCCHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHH
DKTQKLEECRGIEIGHIFQLGTKYSKSLNATFTNEKGIEDHLWMGCYGIGISRLAQAAVE
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
QNHDDLGIIWPTSIAPFTVIIIIANIKNNDQKCLAEDIYQKLIQNRVDVLLDDRDDRAGI
CCCCCEEEEECCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
KFKDADLIGIPWRIVAGREASSGLVELHNRKTKTTELLDLNSVLKKLSEEFNTEKL
EECCCCEECCCEEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA