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The map label for this gene is cobM [H]

Identifier: 124025225

GI number: 124025225

Start: 468769

End: 469527

Strand: Reverse

Name: cobM [H]

Synonym: NATL1_05141

Alternate gene names: 124025225

Gene position: 469527-468769 (Counterclockwise)

Preceding gene: 124025226

Following gene: 124025224

Centisome position: 25.18

GC content: 35.57

Gene sequence:

>759_bases
ATGAATCCGATTTCAATTGTTGGCGCAGGTCCAGGCGCTTTAGACTTAATGACAATAAGAGCTCAACAAAGAATTAAATC
AGCAGATGTTCTTCTTTGGACAGACTCTCTCATTCCTATACAAATAACAAATTTTGTTAAAGATGATTGTGAAAAGATAA
AGACAAGTTCATTAACACTAGAAGAAATACTTTTAATCTTAATTAAAAAGCATAAGGAAGGTAAAAAAATTGTTCGTCTC
CATGATGGTGATCCTTGCTTATATGGAGCCATATCAGAACAAATATGCAGATTAAATGATGAAGGGATTGAGGTAGAAGT
AGTACCGGGAGTAAGCGCATATCAAGCAACAGCAGCAACTTTAGGATTCGAACTAACAGTTCCAGATTTAACCCAAACAA
TAATACTAAGCAGAGCTGATGGTAGGACAGGAAAACCAGAGAGGGAGAGCCTTCAAAAGCTAGCATCTATTCAATCTTCT
TTATGTCTCTACCTAAGTGCAAGACATGTGGAAGAAGTGCAATCCATACTTATAAAGTATTATCCTGCGAACACCCCTGT
AGCAATTGCATATAGAGTAACTTGGCCAGACGAATGGATAAAAGTCATACCTTTGAATGAGATGGCAAAAACATCTCAAG
AACAAAATTTGATCAGAACAACTTTATATATAATCAGTCCAACTCTAAAGATCGGTAATAATAGATCAAAGCTTTATAAT
CCTACTCATTCGCATTTATTTAGGTCGAGTTCAAGATAA

Upstream 100 bases:

>100_bases
TTAGCTTATTTTTAATTAGTGCAGGTTTATTAGGAATATGGAGAATTTATGTTTCTAAAAAAGCGCTCCCCGATCCTTCC
TCAATTAATGGGAGAAATCA

Downstream 100 bases:

>100_bases
AAACTAAGGACTGTATTTTAACAATGACTAGAGCTAGAGATAAATTATCTTTTAGAGACAAACGACTTTACAGAGCCTTT
AGAATAATCTAAATACCCAA

Product: putative precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MNPISIVGAGPGALDLMTIRAQQRIKSADVLLWTDSLIPIQITNFVKDDCEKIKTSSLTLEEILLILIKKHKEGKKIVRL
HDGDPCLYGAISEQICRLNDEGIEVEVVPGVSAYQATAATLGFELTVPDLTQTIILSRADGRTGKPERESLQKLASIQSS
LCLYLSARHVEEVQSILIKYYPANTPVAIAYRVTWPDEWIKVIPLNEMAKTSQEQNLIRTTLYIISPTLKIGNNRSKLYN
PTHSHLFRSSSR

Sequences:

>Translated_252_residues
MNPISIVGAGPGALDLMTIRAQQRIKSADVLLWTDSLIPIQITNFVKDDCEKIKTSSLTLEEILLILIKKHKEGKKIVRL
HDGDPCLYGAISEQICRLNDEGIEVEVVPGVSAYQATAATLGFELTVPDLTQTIILSRADGRTGKPERESLQKLASIQSS
LCLYLSARHVEEVQSILIKYYPANTPVAIAYRVTWPDEWIKVIPLNEMAKTSQEQNLIRTTLYIISPTLKIGNNRSKLYN
PTHSHLFRSSSR
>Mature_252_residues
MNPISIVGAGPGALDLMTIRAQQRIKSADVLLWTDSLIPIQITNFVKDDCEKIKTSSLTLEEILLILIKKHKEGKKIVRL
HDGDPCLYGAISEQICRLNDEGIEVEVVPGVSAYQATAATLGFELTVPDLTQTIILSRADGRTGKPERESLQKLASIQSS
LCLYLSARHVEEVQSILIKYYPANTPVAIAYRVTWPDEWIKVIPLNEMAKTSQEQNLIRTTLYIISPTLKIGNNRSKLYN
PTHSHLFRSSSR

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=220, Percent_Identity=30.4545454545455, Blast_Score=103, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6322922, Length=212, Percent_Identity=28.3018867924528, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28123; Mature: 28123

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPISIVGAGPGALDLMTIRAQQRIKSADVLLWTDSLIPIQITNFVKDDCEKIKTSSLTL
CCCEEEEECCCCCCHHEEHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHCCCCH
EEILLILIKKHKEGKKIVRLHDGDPCLYGAISEQICRLNDEGIEVEVVPGVSAYQATAAT
HHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
LGFELTVPDLTQTIILSRADGRTGKPERESLQKLASIQSSLCLYLSARHVEEVQSILIKY
EEEEEECCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
YPANTPVAIAYRVTWPDEWIKVIPLNEMAKTSQEQNLIRTTLYIISPTLKIGNNRSKLYN
CCCCCCEEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHEEEEEECEEEECCCCHHCCC
PTHSHLFRSSSR
CHHHHHHCCCCC
>Mature Secondary Structure
MNPISIVGAGPGALDLMTIRAQQRIKSADVLLWTDSLIPIQITNFVKDDCEKIKTSSLTL
CCCEEEEECCCCCCHHEEHHHHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHHCCCCH
EEILLILIKKHKEGKKIVRLHDGDPCLYGAISEQICRLNDEGIEVEVVPGVSAYQATAAT
HHHHHHHHHCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHH
LGFELTVPDLTQTIILSRADGRTGKPERESLQKLASIQSSLCLYLSARHVEEVQSILIKY
EEEEEECCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
YPANTPVAIAYRVTWPDEWIKVIPLNEMAKTSQEQNLIRTTLYIISPTLKIGNNRSKLYN
CCCCCCEEEEEEEECCCCEEEEEECHHHHHHHHHHHHHHHEEEEEECEEEECCCCHHCCC
PTHSHLFRSSSR
CHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]