Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 124025154

GI number: 124025154

Start: 404273

End: 405265

Strand: Reverse

Name: dus1 [H]

Synonym: NATL1_04411

Alternate gene names: 124025154

Gene position: 405265-404273 (Counterclockwise)

Preceding gene: 124025155

Following gene: 124025144

Centisome position: 21.73

GC content: 40.08

Gene sequence:

>993_bases
ATGAAAGAGTTTTCTCCGATTTGTTTATCAGGCAATGGGACGCCTAGATCTATAGAGTGTCTGGTTATTCAGTCCCCACT
TGCAGGAGTGAGCGATCAAATATTCAGGAACTTTGTTCGTAGATGGTCTCCAAAAGCTTTACTTTTCACCGAAATGGTAA
ATGCTAAAAGTCTTGAATTAGGTCATGGTGAAGAGAAAGTAATTGAGCTTTCAGAAGAAAGTGGTCCAATTGGTGTTCAA
CTTTTTGACCATAGGCCAGATTCAATGGTAGATGCTGCCATCAAAGCCGAATCATCTGGTGCATTTCTTATAGATATCAA
TATGGGTTGCCCAGTAAAAAAAATTGCCAGGAAAGGAGGCGGAAGTGCTCTATTGAAAGAACCAGAACTTGCGCAATTAA
TCGTCAAAAAGGTTTCAAAAGCCATATCTATTCCAGTAACAGTCAAAATAAGATTGGGGTGGTGTGAAACCACAAGTGAT
CCAGTATCTTTTGCTTTAGGTCTACAGGAGGCTGGGGCTCAACTCATAACTGTTCATGGGCGAACAAGAAGGCAAGGATT
TTCTGGCCATTCAAACTGGAAAGCTATTGCCCAAATCAAAAAGTCATTAGATATACCTGTCATCGCTAATGGTGATATTA
AAAACTCTCGAGATGCTATTGAGTGCCTAAAGATCACGAATGCCGATGGGGTGATGATAGGAAGGGCAAGTATGGGAGCT
CCATGGCTGGTTGGACAAATTGATGAAGAAATTAAAAACCAAACAACTTTTAAACCACCTGACGCAAAGATGAAAGTGAG
CTTATCTTTAGAGCACCTAAAATTACTTGTTTCAAAGAAAGGAAGTCATGGGCTTTTGATTGCTAGGAAACATATGAATT
GGACTTGTAGAGGTTTTGAGGGTGCGTCTACTCTTCGCCATAAATTAGTTAGAGCAAGCACTCCAAACGACGCAATTAAA
CTACTGGAAGACGAACTGATTAAATTCAACTAA

Upstream 100 bases:

>100_bases
AGCATCAATAAATATTTTTTGCTTATTTTCAGAAATCAGCGAAACAGATAAAACTTTCTCTTTATGTAATTACTGAAATA
AATCAAATTTTCCTCTAAAG

Downstream 100 bases:

>100_bases
AAAGTTTTAGGGCATTGTCAAAGGCCAACTTTGTCGAATCAGCAGTAAAGAAAAATATGGCCTAGTCCCTGAGGGTAGGT
CAGATGCCCTAAGTATTTGT

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKEFSPICLSGNGTPRSIECLVIQSPLAGVSDQIFRNFVRRWSPKALLFTEMVNAKSLELGHGEEKVIELSEESGPIGVQ
LFDHRPDSMVDAAIKAESSGAFLIDINMGCPVKKIARKGGGSALLKEPELAQLIVKKVSKAISIPVTVKIRLGWCETTSD
PVSFALGLQEAGAQLITVHGRTRRQGFSGHSNWKAIAQIKKSLDIPVIANGDIKNSRDAIECLKITNADGVMIGRASMGA
PWLVGQIDEEIKNQTTFKPPDAKMKVSLSLEHLKLLVSKKGSHGLLIARKHMNWTCRGFEGASTLRHKLVRASTPNDAIK
LLEDELIKFN

Sequences:

>Translated_330_residues
MKEFSPICLSGNGTPRSIECLVIQSPLAGVSDQIFRNFVRRWSPKALLFTEMVNAKSLELGHGEEKVIELSEESGPIGVQ
LFDHRPDSMVDAAIKAESSGAFLIDINMGCPVKKIARKGGGSALLKEPELAQLIVKKVSKAISIPVTVKIRLGWCETTSD
PVSFALGLQEAGAQLITVHGRTRRQGFSGHSNWKAIAQIKKSLDIPVIANGDIKNSRDAIECLKITNADGVMIGRASMGA
PWLVGQIDEEIKNQTTFKPPDAKMKVSLSLEHLKLLVSKKGSHGLLIARKHMNWTCRGFEGASTLRHKLVRASTPNDAIK
LLEDELIKFN
>Mature_330_residues
MKEFSPICLSGNGTPRSIECLVIQSPLAGVSDQIFRNFVRRWSPKALLFTEMVNAKSLELGHGEEKVIELSEESGPIGVQ
LFDHRPDSMVDAAIKAESSGAFLIDINMGCPVKKIARKGGGSALLKEPELAQLIVKKVSKAISIPVTVKIRLGWCETTSD
PVSFALGLQEAGAQLITVHGRTRRQGFSGHSNWKAIAQIKKSLDIPVIANGDIKNSRDAIECLKITNADGVMIGRASMGA
PWLVGQIDEEIKNQTTFKPPDAKMKVSLSLEHLKLLVSKKGSHGLLIARKHMNWTCRGFEGASTLRHKLVRASTPNDAIK
LLEDELIKFN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=228, Percent_Identity=29.3859649122807, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI239788462, Length=228, Percent_Identity=29.3859649122807, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=33.4801762114537, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI31742496, Length=234, Percent_Identity=31.6239316239316, Blast_Score=101, Evalue=9e-22,
Organism=Homo sapiens, GI8923374, Length=142, Percent_Identity=35.2112676056338, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789660, Length=312, Percent_Identity=34.6153846153846, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788462, Length=311, Percent_Identity=31.5112540192926, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI145693211, Length=303, Percent_Identity=27.7227722772277, Blast_Score=97, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=233, Percent_Identity=30.4721030042918, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=226, Percent_Identity=28.3185840707965, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25144369, Length=217, Percent_Identity=30.8755760368664, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=236, Percent_Identity=31.3559322033898, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=263, Percent_Identity=28.8973384030418, Blast_Score=83, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=28.1385281385281, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585320, Length=244, Percent_Identity=32.3770491803279, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24580595, Length=236, Percent_Identity=32.6271186440678, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19920448, Length=236, Percent_Identity=32.6271186440678, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=31.0043668122271, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI45549423, Length=143, Percent_Identity=37.7622377622378, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36006; Mature: 36006

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFSPICLSGNGTPRSIECLVIQSPLAGVSDQIFRNFVRRWSPKALLFTEMVNAKSLEL
CCCCCCEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEHHCCCCEEEC
GHGEEKVIELSEESGPIGVQLFDHRPDSMVDAAIKAESSGAFLIDINMGCPVKKIARKGG
CCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHEECCCCEEEEEECCCCCHHHHHHCCC
GSALLKEPELAQLIVKKVSKAISIPVTVKIRLGWCETTSDPVSFALGLQEAGAQLITVHG
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHEEECHHHCCCEEEEEEC
RTRRQGFSGHSNWKAIAQIKKSLDIPVIANGDIKNSRDAIECLKITNADGVMIGRASMGA
CHHCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCC
PWLVGQIDEEIKNQTTFKPPDAKMKVSLSLEHLKLLVSKKGSHGLLIARKHMNWTCRGFE
CEEEHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCEEEEEECCCEEEECCC
GASTLRHKLVRASTPNDAIKLLEDELIKFN
CHHHHHHHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKEFSPICLSGNGTPRSIECLVIQSPLAGVSDQIFRNFVRRWSPKALLFTEMVNAKSLEL
CCCCCCEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEHHCCCCEEEC
GHGEEKVIELSEESGPIGVQLFDHRPDSMVDAAIKAESSGAFLIDINMGCPVKKIARKGG
CCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHEECCCCEEEEEECCCCCHHHHHHCCC
GSALLKEPELAQLIVKKVSKAISIPVTVKIRLGWCETTSDPVSFALGLQEAGAQLITVHG
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHEEECHHHCCCEEEEEEC
RTRRQGFSGHSNWKAIAQIKKSLDIPVIANGDIKNSRDAIECLKITNADGVMIGRASMGA
CHHCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCC
PWLVGQIDEEIKNQTTFKPPDAKMKVSLSLEHLKLLVSKKGSHGLLIARKHMNWTCRGFE
CEEEHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHCCCCCCEEEEEECCCEEEECCC
GASTLRHKLVRASTPNDAIKLLEDELIKFN
CHHHHHHHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]