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The map label for this gene is metK

Identifier: 124025113

GI number: 124025113

Start: 369272

End: 370501

Strand: Reverse

Name: metK

Synonym: NATL1_04001

Alternate gene names: 124025113

Gene position: 370501-369272 (Counterclockwise)

Preceding gene: 124025114

Following gene: 124025112

Centisome position: 19.87

GC content: 41.38

Gene sequence:

>1230_bases
ATGAGTAGATACGTTTTCACATCTGAATCTGTCACTGAAGGACATCCAGACAAAATCTGTGATCAAGTGAGTGACGCAGT
TTTAGATGCATGCCTAAGTGAAGATCCAACCAGCAGAGTCGCATGTGAAGCAGTTGTTAATACTGGCTTATGTTTAATAA
CTGGAGAAATAACTTCAAAAGCAGAGGTGGATTTTAATAAGCTTGTCAGAGAAGTTATCAAAAGCATTGGATATAGAAGT
GCAAGTGATGGCGGATTCGATGCTAATAGTTGTGCCGTTCTAGTCGCACTTGACCAACAGTCTTCAGATATAGCTCAGGG
TGTAAATGAAGCTGAAGACCATTCAACAGATCCGCTCGATCAAGTAGGGGCTGGTGATCAGGGGATCATGTTTGGATTTG
CTTGTGACGAGACTCCTGAACTTATGCCATTGCCAATAAGTCTCGCTCATCGATTAGCACGAAGGCTGGCATTAGTTAGG
CACCAACAATTAATCGATTATCTTTTACCTGATGGCAAAACCCAAGTAAGTGTCTCATACGAAAACGGAGTACCATGCTC
CATAGACACAATACTGATTTCTACTCAGCATAAATCCGAAGTAGATGGAATAACTCTTGAAGAGGAAATTCAAAAAAGAA
TTGCTAAAGACCTATGGAAATTTGTTGTTGAGCCCGCTACCGAAGACCTGCCTCTGAAACCTGCTAAAGAATCAACTCGC
TTCCTTGTTAATCCCACAGGGAAATTTGTTATCGGGGGACCCCAAGGCGACGCAGGTCTAACTGGAAGAAAAATAATTGT
TGATACCTACGGTGGATATGCTCGACATGGTGGAGGCGCTTTTTCAGGGAAAGACCCTACTAAGGTTGATAGATCTGCAG
CTTATGCTGCAAGATTTGTTGCGAAGGCATTAGTTGCAGCAAATCTTGCAAAAAAAGTTGAAGTTCAATTAAGTTATGCA
ATCGGAGTCGCAAAACCTATATCGATACTTGTGGATTCATTTGGGACAGGAAAGGTTTCAGACTCTGAACTAACACAACT
TGTACAAGATCAATTCGATCTAAGACCAGGTGCAATTATTAAAGCATTCGATCTTCAAAACCTACCAAGTGTTAGAGGTG
GACGTTTTTATAGAGATACTGCGGCCTATGGTCATTTTGGCAGAACGGATATTCTTCTCCCATGGGAGGACGTATCTCAA
AAAGCAAAAGAACTAAGTTTACTCAAATAA

Upstream 100 bases:

>100_bases
AAACCCCCCCAGTTCTGACTGAAAAAAAATTTCTTATAGAAAAATTAAATGAATTAAAAATTCATTCAAGCTATTAAAAT
ATTGAATATTAATATTTTAA

Downstream 100 bases:

>100_bases
AAAGAATTTAATAAAAAAAAATGTTAAACAATTCTTTTGTACTTGGTATAGATCTTGGTACATCAGGCGTAAGGATTGCA
ATCATTAATACTAAAAAAAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSRYVFTSESVTEGHPDKICDQVSDAVLDACLSEDPTSRVACEAVVNTGLCLITGEITSKAEVDFNKLVREVIKSIGYRS
ASDGGFDANSCAVLVALDQQSSDIAQGVNEAEDHSTDPLDQVGAGDQGIMFGFACDETPELMPLPISLAHRLARRLALVR
HQQLIDYLLPDGKTQVSVSYENGVPCSIDTILISTQHKSEVDGITLEEEIQKRIAKDLWKFVVEPATEDLPLKPAKESTR
FLVNPTGKFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKALVAANLAKKVEVQLSYA
IGVAKPISILVDSFGTGKVSDSELTQLVQDQFDLRPGAIIKAFDLQNLPSVRGGRFYRDTAAYGHFGRTDILLPWEDVSQ
KAKELSLLK

Sequences:

>Translated_409_residues
MSRYVFTSESVTEGHPDKICDQVSDAVLDACLSEDPTSRVACEAVVNTGLCLITGEITSKAEVDFNKLVREVIKSIGYRS
ASDGGFDANSCAVLVALDQQSSDIAQGVNEAEDHSTDPLDQVGAGDQGIMFGFACDETPELMPLPISLAHRLARRLALVR
HQQLIDYLLPDGKTQVSVSYENGVPCSIDTILISTQHKSEVDGITLEEEIQKRIAKDLWKFVVEPATEDLPLKPAKESTR
FLVNPTGKFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKALVAANLAKKVEVQLSYA
IGVAKPISILVDSFGTGKVSDSELTQLVQDQFDLRPGAIIKAFDLQNLPSVRGGRFYRDTAAYGHFGRTDILLPWEDVSQ
KAKELSLLK
>Mature_408_residues
SRYVFTSESVTEGHPDKICDQVSDAVLDACLSEDPTSRVACEAVVNTGLCLITGEITSKAEVDFNKLVREVIKSIGYRSA
SDGGFDANSCAVLVALDQQSSDIAQGVNEAEDHSTDPLDQVGAGDQGIMFGFACDETPELMPLPISLAHRLARRLALVRH
QQLIDYLLPDGKTQVSVSYENGVPCSIDTILISTQHKSEVDGITLEEEIQKRIAKDLWKFVVEPATEDLPLKPAKESTRF
LVNPTGKFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFVAKALVAANLAKKVEVQLSYAI
GVAKPISILVDSFGTGKVSDSELTQLVQDQFDLRPGAIIKAFDLQNLPSVRGGRFYRDTAAYGHFGRTDILLPWEDVSQK
AKELSLLK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=397, Percent_Identity=54.6599496221663, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI5174529, Length=404, Percent_Identity=53.2178217821782, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1789311, Length=411, Percent_Identity=54.7445255474453, Blast_Score=435, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17551082, Length=399, Percent_Identity=53.8847117794486, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538494, Length=411, Percent_Identity=52.3114355231144, Blast_Score=388, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17538498, Length=398, Percent_Identity=53.5175879396985, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=399, Percent_Identity=53.3834586466165, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565905, Length=411, Percent_Identity=46.4720194647202, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI32565911, Length=351, Percent_Identity=51.5669515669516, Blast_Score=314, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI25145635, Length=351, Percent_Identity=51.5669515669516, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI32565903, Length=349, Percent_Identity=47.5644699140401, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=63.4146341463415, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=70, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=397, Percent_Identity=52.896725440806, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6320710, Length=397, Percent_Identity=53.1486146095718, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45552159, Length=398, Percent_Identity=50.7537688442211, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580529, Length=398, Percent_Identity=50.7537688442211, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580537, Length=398, Percent_Identity=50.7537688442211, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580533, Length=398, Percent_Identity=50.7537688442211, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580535, Length=398, Percent_Identity=50.7537688442211, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580531, Length=398, Percent_Identity=50.7537688442211, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580527, Length=398, Percent_Identity=50.251256281407, Blast_Score=351, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580525, Length=398, Percent_Identity=50.251256281407, Blast_Score=351, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24580541, Length=385, Percent_Identity=49.3506493506493, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24580539, Length=124, Percent_Identity=55.6451612903226, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_PROM1 (A2C0F4)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014229.1
- ProteinModelPortal:   A2C0F4
- SMR:   A2C0F4
- STRING:   A2C0F4
- GeneID:   4780897
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_04001
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   PMAR167555:NATL1_04001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44134; Mature: 44003

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYVFTSESVTEGHPDKICDQVSDAVLDACLSEDPTSRVACEAVVNTGLCLITGEITSK
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCC
AEVDFNKLVREVIKSIGYRSASDGGFDANSCAVLVALDQQSSDIAQGVNEAEDHSTDPLD
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHCCCHHHCCCCCCHH
QVGAGDQGIMFGFACDETPELMPLPISLAHRLARRLALVRHQQLIDYLLPDGKTQVSVSY
HHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
ENGVPCSIDTILISTQHKSEVDGITLEEEIQKRIAKDLWKFVVEPATEDLPLKPAKESTR
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
FLVNPTGKFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKALVAANLAKKVEVQLSYAIGVAKPISILVDSFGTGKVSDSELTQLVQDQFDLRPGAII
HHHHHHHHHHHHHEEEEEEHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEE
KAFDLQNLPSVRGGRFYRDTAAYGHFGRTDILLPWEDVSQKAKELSLLK
EEECCCCCCCCCCCCEEEHHHHCCCCCCCEEEECHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRYVFTSESVTEGHPDKICDQVSDAVLDACLSEDPTSRVACEAVVNTGLCLITGEITSK
CCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCEEEEECCCCCC
AEVDFNKLVREVIKSIGYRSASDGGFDANSCAVLVALDQQSSDIAQGVNEAEDHSTDPLD
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHCCCHHHCCCCCCHH
QVGAGDQGIMFGFACDETPELMPLPISLAHRLARRLALVRHQQLIDYLLPDGKTQVSVSY
HHCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
ENGVPCSIDTILISTQHKSEVDGITLEEEIQKRIAKDLWKFVVEPATEDLPLKPAKESTR
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
FLVNPTGKFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARFV
EEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AKALVAANLAKKVEVQLSYAIGVAKPISILVDSFGTGKVSDSELTQLVQDQFDLRPGAII
HHHHHHHHHHHHHEEEEEEHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEE
KAFDLQNLPSVRGGRFYRDTAAYGHFGRTDILLPWEDVSQKAKELSLLK
EEECCCCCCCCCCCCEEEHHHHCCCCCCCEEEECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA