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The map label for this gene is ispD [H]

Identifier: 124022365

GI number: 124022365

Start: 616861

End: 617607

Strand: Direct

Name: ispD [H]

Synonym: P9303_06551

Alternate gene names: 124022365

Gene position: 616861-617607 (Clockwise)

Preceding gene: 124022361

Following gene: 124022366

Centisome position: 22.99

GC content: 55.69

Gene sequence:

>747_bases
TTGCGAGCTGATTGTATGGAGGAGGTTTCAGCCTGCTTGCGGCAGGAGGCGCATATGCATCTGTTGATTGTCGCTGCAGG
AAGCGGAAGCCGTATGGGAGCGGATCGCAACAAATTGCTTTTGCCTCTGGCAGGACGCCCTGTTCTTGCTTGGACGATTG
ATGCGGTGATGGGAGCCGATTCGATCACTTGGGTTGGCATTGTTGGCCAACCTGTTGACCGAGCAATGATCATGGAGTTG
TTGGCTGAGGCAGCCAAACCCGTTGTTTGGATTGAGGGGGGCAGCACAAGGCAGGAATCCGTAGAGCGTGGATTACAGGC
GTTGCCTTCAGTTGCGCAGCATGTTCTTATTCATGATGGGGCTAGATGTTTAGCTGAAGCGGCTTTGATCAATCGCTGTG
CTGAGGCGGTGGTGGCTGGCGAAGCGGTGATTGCGGCAACTCCTGTTACCGACACGATCAAGCGAGTTGATGGTCAAGGG
ATCATTACGGGCACACCAGATCGTGCGGAACTTTGGGCAGCCCAGACTCCGCAGGGCTTTGCTGTGGAGCAGCTCAAGCA
AGGTCATGCTGAGGCTCAGGCCAAGGGTTGGACAGTGACTGACGATGCCTCGCTTTATGAACGCCTGGGATGGCCGGTGC
AGGTGTTGGAGGCCAGCCCTGCCAATATCAAGGTGACTACACCGTTTGACCTCACGGTGGCCGAAGCAGTGCTTGCGCTT
AGGGCAAAAAGGCAGATAGGTCTCTAG

Upstream 100 bases:

>100_bases
CTGGTTGGCTGCGGCGGCTAGGGCTGGAAGCCTCTGAAGCTGTTGGATTAAAGGACCCGGACTGAGGGCGAGAACACGCA
TCGATCCAGAGGCAGAGGCG

Downstream 100 bases:

>100_bases
AAAGTGAGCTATAAATTAGAAGCAGTGAAGCATGAGAAATTGCTCAAGGAACTGACCCCTATTTCTCTTCTTATAAGTGG
AATATTATTTCCAGCAGACG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MRADCMEEVSACLRQEAHMHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMGADSITWVGIVGQPVDRAMIMEL
LAEAAKPVVWIEGGSTRQESVERGLQALPSVAQHVLIHDGARCLAEAALINRCAEAVVAGEAVIAATPVTDTIKRVDGQG
IITGTPDRAELWAAQTPQGFAVEQLKQGHAEAQAKGWTVTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVLAL
RAKRQIGL

Sequences:

>Translated_248_residues
MRADCMEEVSACLRQEAHMHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMGADSITWVGIVGQPVDRAMIMEL
LAEAAKPVVWIEGGSTRQESVERGLQALPSVAQHVLIHDGARCLAEAALINRCAEAVVAGEAVIAATPVTDTIKRVDGQG
IITGTPDRAELWAAQTPQGFAVEQLKQGHAEAQAKGWTVTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVLAL
RAKRQIGL
>Mature_248_residues
MRADCMEEVSACLRQEAHMHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMGADSITWVGIVGQPVDRAMIMEL
LAEAAKPVVWIEGGSTRQESVERGLQALPSVAQHVLIHDGARCLAEAALINRCAEAVVAGEAVIAATPVTDTIKRVDGQG
IITGTPDRAELWAAQTPQGFAVEQLKQGHAEAQAKGWTVTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVLAL
RAKRQIGL

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=237, Percent_Identity=24.4725738396624, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789104, Length=219, Percent_Identity=36.5296803652968, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26365; Mature: 26365

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRADCMEEVSACLRQEAHMHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMGAD
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEHHHHHCCC
SITWVGIVGQPVDRAMIMELLAEAAKPVVWIEGGSTRQESVERGLQALPSVAQHVLIHDG
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCH
ARCLAEAALINRCAEAVVAGEAVIAATPVTDTIKRVDGQGIITGTPDRAELWAAQTPQGF
HHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCEEEECCCCHHHHEECCCCCCC
AVEQLKQGHAEAQAKGWTVTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVLAL
HHHHHHHCHHHHHCCCCEECCCHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHH
RAKRQIGL
HHHHHCCC
>Mature Secondary Structure
MRADCMEEVSACLRQEAHMHLLIVAAGSGSRMGADRNKLLLPLAGRPVLAWTIDAVMGAD
CCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCEEEEEHHHHHCCC
SITWVGIVGQPVDRAMIMELLAEAAKPVVWIEGGSTRQESVERGLQALPSVAQHVLIHDG
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCH
ARCLAEAALINRCAEAVVAGEAVIAATPVTDTIKRVDGQGIITGTPDRAELWAAQTPQGF
HHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCEEEECCCCHHHHEECCCCCCC
AVEQLKQGHAEAQAKGWTVTDDASLYERLGWPVQVLEASPANIKVTTPFDLTVAEAVLAL
HHHHHHHCHHHHHCCCCEECCCHHHHHHCCCCEEEEECCCCCEEEECCCCHHHHHHHHHH
RAKRQIGL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]