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The map label for this gene is birA [H]

Identifier: 124022333

GI number: 124022333

Start: 587284

End: 588042

Strand: Direct

Name: birA [H]

Synonym: P9303_06231

Alternate gene names: 124022333

Gene position: 587284-588042 (Clockwise)

Preceding gene: 124022332

Following gene: 124022335

Centisome position: 21.89

GC content: 58.37

Gene sequence:

>759_bases
ATGCGCATATCTGAGTCATGGACTGGTGCTGCTGCGGTTGCCCGTTTGTGGCGTAGGCAGTCTTGCGGGGCTAGGCCATG
GCAGTTGCGATGGCAGCCGGTCTGTGCGAGTACTGAGTTGATGTTGTCTGGTTGGCTGAAGCAGCAGCCTTGTTCAGCTC
AGCAGCCTCGGGCCTTGTTTGCTGATCGCCAGACCCATGGGAGGGGACAGCGTGGTCGGGTCTGGCAGTCACCGATCGGT
GGGGTTTGGATCAGTGCCGCTTTGCCCTGGTTTGATGCTCAGTGCTCTGCCGGTATTTTTGGAGTAGCAGTGGCTGTGGC
GCTGGCTGAAAGGCTCGAGCGGCGGGGAGTTCCCGTGGGTATCAAGTGGCCTAACGATTTGATGGTGGGGAACCGTAAAT
TGGCTGGTCTCTTGCCGAGATTGGTGCACCGTGGCAGTCGCGTCAGGCTGGCACGCATTGGTCTTGGCCTGAATGTTTGC
AACCGTGTGCCCAGAGAAGGGATTGCCTTAGATGAGTTGCTTCGATCAGGCCAGTGTCAGCCTTTGGTCTGGATGGCAGA
AGTGCTATGCGCTCTTGATCGAACCATGGATTTGGCTGGTCGAGCGGATTGGGTTTGCGCCGAAGCTGAGCGACGCCTTT
GGACAAAGCAGGTTCGTGATCCGAAGGGTGGTGAACTCTGGGACGTGATCGGACTGGGACTGGATGGGTCGCTTTTGCTG
AGCCAGGGTTCTCGGACCATGCGCTGGACACGCTGGTGA

Upstream 100 bases:

>100_bases
TCAGGTTTTGGCTCCGGCGGGGATGGTTGGCTGCGCCTGGCCCTGGTCCATCCGGTGGAAGATTTGGAGGCGGCAGTTGC
CCGGATGAGGCCTTGGTGGC

Downstream 100 bases:

>100_bases
TAACTGAGTTGGACTTTTTCTATAGTTTTTCTCGTCTGAGGATCCGATCGGGTGCCGTTTCGCTGGTTCGCTGCTGCATG
GCTGCTTTCAGTGCCTGTCC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRISESWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCSAQQPRALFADRQTHGRGQRGRVWQSPIG
GVWISAALPWFDAQCSAGIFGVAVAVALAERLERRGVPVGIKWPNDLMVGNRKLAGLLPRLVHRGSRVRLARIGLGLNVC
NRVPREGIALDELLRSGQCQPLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGLGLDGSLLL
SQGSRTMRWTRW

Sequences:

>Translated_252_residues
MRISESWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCSAQQPRALFADRQTHGRGQRGRVWQSPIG
GVWISAALPWFDAQCSAGIFGVAVAVALAERLERRGVPVGIKWPNDLMVGNRKLAGLLPRLVHRGSRVRLARIGLGLNVC
NRVPREGIALDELLRSGQCQPLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGLGLDGSLLL
SQGSRTMRWTRW
>Mature_252_residues
MRISESWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCSAQQPRALFADRQTHGRGQRGRVWQSPIG
GVWISAALPWFDAQCSAGIFGVAVAVALAERLERRGVPVGIKWPNDLMVGNRKLAGLLPRLVHRGSRVRLARIGLGLNVC
NRVPREGIALDELLRSGQCQPLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGLGLDGSLLL
SQGSRTMRWTRW

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=139, Percent_Identity=33.0935251798561, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR008988 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28163; Mature: 28163

Theoretical pI: Translated: 11.50; Mature: 11.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISESWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCSAQQPRALF
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHH
ADRQTHGRGQRGRVWQSPIGGVWISAALPWFDAQCSAGIFGVAVAVALAERLERRGVPVG
HCCHHCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
IKWPNDLMVGNRKLAGLLPRLVHRGSRVRLARIGLGLNVCNRVPREGIALDELLRSGQCQ
EECCCCCEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHCCCCC
PLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGLGLDGSLLL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHEECCCCCCEEE
SQGSRTMRWTRW
CCCCCCEEECCC
>Mature Secondary Structure
MRISESWTGAAAVARLWRRQSCGARPWQLRWQPVCASTELMLSGWLKQQPCSAQQPRALF
CCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHH
ADRQTHGRGQRGRVWQSPIGGVWISAALPWFDAQCSAGIFGVAVAVALAERLERRGVPVG
HCCHHCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
IKWPNDLMVGNRKLAGLLPRLVHRGSRVRLARIGLGLNVCNRVPREGIALDELLRSGQCQ
EECCCCCEECCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHCCCCC
PLVWMAEVLCALDRTMDLAGRADWVCAEAERRLWTKQVRDPKGGELWDVIGLGLDGSLLL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHEECCCCCCEEE
SQGSRTMRWTRW
CCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]