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The map label for this gene is nadE [H]

Identifier: 124022199

GI number: 124022199

Start: 484294

End: 485988

Strand: Reverse

Name: nadE [H]

Synonym: P9303_04891

Alternate gene names: 124022199

Gene position: 485988-484294 (Counterclockwise)

Preceding gene: 124022200

Following gene: 124022198

Centisome position: 18.12

GC content: 57.17

Gene sequence:

>1695_bases
ATGCGCCTTGCTCTAGCCCAGCTCAATCCACTTGTAGGCGATCTGGCTGGCAACGGCGAAAAGATTCTGGCTGCCTGCCG
TGAGGCTGCTGATCTCGGTGCAGATCTGGTACTCACGCCTGAGCTTTCCCTGTGGGGATATCCACCCCGAGATCTGTTGC
TTAGCCACAACCACCTCGTTCAACAAAGCGATGTGCTCAACCAGATGGCGAGCATCCTTGCCCACGAAGCCCCTGACCTG
GCAGTGCTCGTTGGCCTAGCTGAGCAAGCCCATGACCCTCAACTGCCCCAGCTCTTCAATGCTCTAGCCCTGATCAAAAG
CTCCCAATGGCAGGTGGTAGCTCGCAAGCAACTGCTACCCACCTATGACGTTTTCGATGAAAAACGCTATTTCCGCCCGG
CAGGAACTCCGGCTGTCTTGGAGTTTGAACGCGATGGGCGTCGCTGGCGTCTTGGGCTCACAATCTGCGAAGACCTCTGG
GTGGAAGAAGCACTACAGGGCAAGCGCATTGCCGGCCCTGACCCCATCGCAGCGCTGCTGCCACAAAACATCGACCTACT
GCTTAATCTCTCGGCTTCGCCATTCAGCCAATCCAAAGAACTTCTGCGCCAGCGCCTGGCCGCTAGAGCTGCCCAGAGAC
TGAGCGCTCCGGTGATCTATGTGAATCAAGTGGGCGGCAACGACGAGCTGGTCTTTGATGGCGCCAGTTTCGTCGTCGAT
GAGCATGCTGAACTTGCCCTGCAATTGCCAAGTTGTCGCGAATCAGTAGCCATCTGGGATGCAACCTCTACAGCCACCCA
TCTCGACGTCCCTACGATTGAACCACTAGAGAAGCTATTCCGGGCCTTGGTTCTAGGTGTGCGTGACTACGCCGGCAAAT
GTGGCTTTAAGCGGGCATTGCTTGGCCTCAGCGGTGGTATCGATTCGGCTCTCGTGGCCGTGATCGCTGCAGCGGCCCTA
GGTGCCGATCAGGTGACATCCCTACTGATGCCCTCACCCTGGAGTTCAGCCGGTTCAATTGATGACGCCCTAGCTCTAGC
CAAACGTCTAGGGATGGCGACCCACACTGTGTCGATTGCGACCTTGATGACGAATTTTGAGGCTGCCCTCATCCCTCCTC
TCGGCAAGCCACCCGAGGGCGTTACTGCAGAGAACCTCCAATCCCGCATCCGCGGCACCTTACTGATGGCATTGGCCAAC
CAACAAGACCAGCTGCTGCTATCCACTGGCAACAAATCCGAACTAGCAGTGGGCTACTGCACCCTTTATGGCGATATGAA
CGGCGGCCTAGCCGTGATTGGAGATGTCTACAAAACCAACGTCTTCCGCCTCTGTGATTGGCTTGACAGCCCTGCCGCCG
AAACATGCCGCAAAGAGGTAGGACTACCAACGAAAGGCGAACTGATCGGTACGACAATCCGCCAAAAGCCTCCCAGCGCC
GAATTGCGCCCGAACCAACGCGATAGCGATTCACTACCCGACTACGCCCTCCTCGACCCTCTGCTTAAAGCTCTGATTGA
AGAACGCCTCAGTACGGAGCAACTTGTCAGCAGAGGACATAACCCTGCCCTAATTGGGAGGGTGCAGCAATTACTGCGAC
GGGCCGAATTCAAACGGCGACAGGCCCCACCCCTGCTGAAGGTGAGTTCACAGGCCTTTGGCAGCGGCTGGCGGCTACCG
ATCGCCGCCGAATAA

Upstream 100 bases:

>100_bases
GTTCTGAGGTACGCAGTCAACCCAAGCCGGCCCAGATCCCTGCAGCATTGTGGCCATTAGTTCTGGAACACAACCTCTAC
GGCCTCGCCACCAGTCCCTG

Downstream 100 bases:

>100_bases
TCACCACAAAACCTGGTCAGGCTGCCCTGATGTCGCCAACCTAAAAGAAGTCATTGGCGTCCATGGACTCCTCTATTCTT
TCGGCCCCAATAGCCAGCAT

Product: carbon-nitrogen hydrolase:NAD+ synthase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MRLALAQLNPLVGDLAGNGEKILAACREAADLGADLVLTPELSLWGYPPRDLLLSHNHLVQQSDVLNQMASILAHEAPDL
AVLVGLAEQAHDPQLPQLFNALALIKSSQWQVVARKQLLPTYDVFDEKRYFRPAGTPAVLEFERDGRRWRLGLTICEDLW
VEEALQGKRIAGPDPIAALLPQNIDLLLNLSASPFSQSKELLRQRLAARAAQRLSAPVIYVNQVGGNDELVFDGASFVVD
EHAELALQLPSCRESVAIWDATSTATHLDVPTIEPLEKLFRALVLGVRDYAGKCGFKRALLGLSGGIDSALVAVIAAAAL
GADQVTSLLMPSPWSSAGSIDDALALAKRLGMATHTVSIATLMTNFEAALIPPLGKPPEGVTAENLQSRIRGTLLMALAN
QQDQLLLSTGNKSELAVGYCTLYGDMNGGLAVIGDVYKTNVFRLCDWLDSPAAETCRKEVGLPTKGELIGTTIRQKPPSA
ELRPNQRDSDSLPDYALLDPLLKALIEERLSTEQLVSRGHNPALIGRVQQLLRRAEFKRRQAPPLLKVSSQAFGSGWRLP
IAAE

Sequences:

>Translated_564_residues
MRLALAQLNPLVGDLAGNGEKILAACREAADLGADLVLTPELSLWGYPPRDLLLSHNHLVQQSDVLNQMASILAHEAPDL
AVLVGLAEQAHDPQLPQLFNALALIKSSQWQVVARKQLLPTYDVFDEKRYFRPAGTPAVLEFERDGRRWRLGLTICEDLW
VEEALQGKRIAGPDPIAALLPQNIDLLLNLSASPFSQSKELLRQRLAARAAQRLSAPVIYVNQVGGNDELVFDGASFVVD
EHAELALQLPSCRESVAIWDATSTATHLDVPTIEPLEKLFRALVLGVRDYAGKCGFKRALLGLSGGIDSALVAVIAAAAL
GADQVTSLLMPSPWSSAGSIDDALALAKRLGMATHTVSIATLMTNFEAALIPPLGKPPEGVTAENLQSRIRGTLLMALAN
QQDQLLLSTGNKSELAVGYCTLYGDMNGGLAVIGDVYKTNVFRLCDWLDSPAAETCRKEVGLPTKGELIGTTIRQKPPSA
ELRPNQRDSDSLPDYALLDPLLKALIEERLSTEQLVSRGHNPALIGRVQQLLRRAEFKRRQAPPLLKVSSQAFGSGWRLP
IAAE
>Mature_564_residues
MRLALAQLNPLVGDLAGNGEKILAACREAADLGADLVLTPELSLWGYPPRDLLLSHNHLVQQSDVLNQMASILAHEAPDL
AVLVGLAEQAHDPQLPQLFNALALIKSSQWQVVARKQLLPTYDVFDEKRYFRPAGTPAVLEFERDGRRWRLGLTICEDLW
VEEALQGKRIAGPDPIAALLPQNIDLLLNLSASPFSQSKELLRQRLAARAAQRLSAPVIYVNQVGGNDELVFDGASFVVD
EHAELALQLPSCRESVAIWDATSTATHLDVPTIEPLEKLFRALVLGVRDYAGKCGFKRALLGLSGGIDSALVAVIAAAAL
GADQVTSLLMPSPWSSAGSIDDALALAKRLGMATHTVSIATLMTNFEAALIPPLGKPPEGVTAENLQSRIRGTLLMALAN
QQDQLLLSTGNKSELAVGYCTLYGDMNGGLAVIGDVYKTNVFRLCDWLDSPAAETCRKEVGLPTKGELIGTTIRQKPPSA
ELRPNQRDSDSLPDYALLDPLLKALIEERLSTEQLVSRGHNPALIGRVQQLLRRAEFKRRQAPPLLKVSSQAFGSGWRLP
IAAE

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61043; Mature: 61043

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLALAQLNPLVGDLAGNGEKILAACREAADLGADLVLTPELSLWGYPPRDLLLSHNHLV
CCEEHHHHCHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHCCCHHHH
QQSDVLNQMASILAHEAPDLAVLVGLAEQAHDPQLPQLFNALALIKSSQWQVVARKQLLP
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCC
TYDVFDEKRYFRPAGTPAVLEFERDGRRWRLGLTICEDLWVEEALQGKRIAGPDPIAALL
CHHHHCCHHHCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
PQNIDLLLNLSASPFSQSKELLRQRLAARAAQRLSAPVIYVNQVGGNDELVFDGASFVVD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHEEC
EHAELALQLPSCRESVAIWDATSTATHLDVPTIEPLEKLFRALVLGVRDYAGKCGFKRAL
CCHHHEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LGLSGGIDSALVAVIAAAALGADQVTSLLMPSPWSSAGSIDDALALAKRLGMATHTVSIA
HHHCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
TLMTNFEAALIPPLGKPPEGVTAENLQSRIRGTLLMALANQQDQLLLSTGNKSELAVGYC
HHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEE
TLYGDMNGGLAVIGDVYKTNVFRLCDWLDSPAAETCRKEVGLPTKGELIGTTIRQKPPSA
EEEECCCCCEEEECHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCHHHCCCCCC
ELRPNQRDSDSLPDYALLDPLLKALIEERLSTEQLVSRGHNPALIGRVQQLLRRAEFKRR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QAPPLLKVSSQAFGSGWRLPIAAE
CCCCCEEECCHHCCCCCCCCCCCC
>Mature Secondary Structure
MRLALAQLNPLVGDLAGNGEKILAACREAADLGADLVLTPELSLWGYPPRDLLLSHNHLV
CCEEHHHHCHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHCCCHHHH
QQSDVLNQMASILAHEAPDLAVLVGLAEQAHDPQLPQLFNALALIKSSQWQVVARKQLLP
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCC
TYDVFDEKRYFRPAGTPAVLEFERDGRRWRLGLTICEDLWVEEALQGKRIAGPDPIAALL
CHHHHCCHHHCCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
PQNIDLLLNLSASPFSQSKELLRQRLAARAAQRLSAPVIYVNQVGGNDELVFDGASFVVD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCHHEEC
EHAELALQLPSCRESVAIWDATSTATHLDVPTIEPLEKLFRALVLGVRDYAGKCGFKRAL
CCHHHEEECCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LGLSGGIDSALVAVIAAAALGADQVTSLLMPSPWSSAGSIDDALALAKRLGMATHTVSIA
HHHCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHH
TLMTNFEAALIPPLGKPPEGVTAENLQSRIRGTLLMALANQQDQLLLSTGNKSELAVGYC
HHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEE
TLYGDMNGGLAVIGDVYKTNVFRLCDWLDSPAAETCRKEVGLPTKGELIGTTIRQKPPSA
EEEECCCCCEEEECHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEECCHHHCCCCCC
ELRPNQRDSDSLPDYALLDPLLKALIEERLSTEQLVSRGHNPALIGRVQQLLRRAEFKRR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QAPPLLKVSSQAFGSGWRLPIAAE
CCCCCEEECCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]