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The map label for this gene is nusA [H]

Identifier: 124022125

GI number: 124022125

Start: 415001

End: 416455

Strand: Reverse

Name: nusA [H]

Synonym: P9303_04151

Alternate gene names: 124022125

Gene position: 416455-415001 (Counterclockwise)

Preceding gene: 124022126

Following gene: 124022124

Centisome position: 15.52

GC content: 55.33

Gene sequence:

>1455_bases
ATGGCTCTCGTTCTACTCCCCGGCCTCAACAACCTGATCGAAGACATCAGCGAGGAGAAGAAACTCCCTACCCAAGTGGT
GGAAGCAGCCCTGCGGGAGGCCCTCCTCAAAGGATATGAACGCTACCGACGCACTCTTTATCTAGGTATCAGTGAGGACC
CTTTCGAAGAAGAGTATTTCAGCAACTTCGATGTTGGACTAGAGCTGGACGATGAAGGTTATCGGGTCCTGGCCAGCAAA
ATCATCGTTGAAGAGGTGGAGAGCGAAGACCACCAAATTGCTCTTCAAGAAGTGATGCAAGTCGCTGAAGACGCCCAGAT
CGGTGACACCGTGGTGCTCGACGTTACCCCCGAGAAGGAAGACTTCGGCCGGATGGCTGCCGCCACAACCAAGCAGGTAC
TGGCGCAAAAGCTACGCGACCAGCAGCGCCGCATGATTCAAGAGGAATTTGCCGATCTAGAAGATCCCGTGCTCACAGCT
CGTGTGATCCGTTTTGAACGTCATTCGGTGATCATGGCCGTGAGTTCTGGTCTTGGGCGCCCTGAAGTGGAGGCCGAGCT
CCCACGCCGCGACCAGCTCCCCAACGACAATTATCGCGCCAACGCCACCTTCAAAGTCTTTCTGAAGGAAGTCAGCGAAG
TGCCCCGACGAGGGCCTCAGTTGTTCGTTAGCCGCTCCAACGCCGGACTAGTGGTTTACCTGTTTGAGAACGAGGTGCCC
GAAATCCAAGAAGGCTCAGTGCGCATTGTGGCCGTAGCCCGTGAAGCAAATCCTCCGTCTCGTTCCGTGGGCCCACGCAC
CAAGGTGGCAGTTGACAGTATTGAACGTGAAGTGGACCCTGTCGGCGCCTGCATCGGTGCCCGCGGCTCACGCATTCAGC
AAGTGGTTAATGAACTACGCGGCGAAAAAATCGATGTGATCCGCTGGTCACCTGACCCGGGTCAATACATTGCCAATTCC
CTTAGCCCTGCTCGCGTTGAGATGGTGCGACTGGTGGATCCAGAAGGGCAGCATGCCCACGTCCTAGTCCCCCCAGATCA
ACTAAGCCTGGCCATCGGACGAGAAGGACAAAATGTACGCCTAGCAGCTCGTCTAACCGGATGGAAAATCGACATCAAAA
ACTCCCAGGAATATGACCAGGCCAGTGAGGACACCACCGTCGCCGAGCTGATTTCTCAGAGAGAGGAAGAAGAGGCTCTC
CAACGCGATGCCGAATCCCGTTTGGCTGCTGAACAAGCCACCCGAGCAGAAGAGGATGCACGCCTAAGAGAGCTTTACCC
CCTACCGGAAGATGAAGAAGAGTACGACCAAGAAGAACCTGCTAAGACGATGGCTGAAGACGAAAATGCATCCGACGCCG
ACGGCCAACCTGACGACTTAAGCAGCCAACCTGACACCTCAAGCGAACAACTCTCAAATGAAGAATCAGTAGAGGAAGAG
GACAGAGCCCGGTGA

Upstream 100 bases:

>100_bases
CACGTAAGGCTGTGACCTGCGTTCGCCTTACAAACCCCACGGGATAAATCACCTTAACGATCAGCTCAGCTTCAGCACCT
TTCCACTCTTTCCATCGTCG

Downstream 100 bases:

>100_bases
ACCAACGCCCCGTCCTGCGTAGCTGCGTGACCTGTCGCCAACTGCTGGATCGCCAGCAGCTTTGGCGGGTGATACGCGAC
CATCAGGAGGGTGTAGTCCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MALVLLPGLNNLIEDISEEKKLPTQVVEAALREALLKGYERYRRTLYLGISEDPFEEEYFSNFDVGLELDDEGYRVLASK
IIVEEVESEDHQIALQEVMQVAEDAQIGDTVVLDVTPEKEDFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTA
RVIRFERHSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSEVPRRGPQLFVSRSNAGLVVYLFENEVP
EIQEGSVRIVAVAREANPPSRSVGPRTKVAVDSIEREVDPVGACIGARGSRIQQVVNELRGEKIDVIRWSPDPGQYIANS
LSPARVEMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSQEYDQASEDTTVAELISQREEEEAL
QRDAESRLAAEQATRAEEDARLRELYPLPEDEEEYDQEEPAKTMAEDENASDADGQPDDLSSQPDTSSEQLSNEESVEEE
DRAR

Sequences:

>Translated_484_residues
MALVLLPGLNNLIEDISEEKKLPTQVVEAALREALLKGYERYRRTLYLGISEDPFEEEYFSNFDVGLELDDEGYRVLASK
IIVEEVESEDHQIALQEVMQVAEDAQIGDTVVLDVTPEKEDFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTA
RVIRFERHSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSEVPRRGPQLFVSRSNAGLVVYLFENEVP
EIQEGSVRIVAVAREANPPSRSVGPRTKVAVDSIEREVDPVGACIGARGSRIQQVVNELRGEKIDVIRWSPDPGQYIANS
LSPARVEMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSQEYDQASEDTTVAELISQREEEEAL
QRDAESRLAAEQATRAEEDARLRELYPLPEDEEEYDQEEPAKTMAEDENASDADGQPDDLSSQPDTSSEQLSNEESVEEE
DRAR
>Mature_483_residues
ALVLLPGLNNLIEDISEEKKLPTQVVEAALREALLKGYERYRRTLYLGISEDPFEEEYFSNFDVGLELDDEGYRVLASKI
IVEEVESEDHQIALQEVMQVAEDAQIGDTVVLDVTPEKEDFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTAR
VIRFERHSVIMAVSSGLGRPEVEAELPRRDQLPNDNYRANATFKVFLKEVSEVPRRGPQLFVSRSNAGLVVYLFENEVPE
IQEGSVRIVAVAREANPPSRSVGPRTKVAVDSIEREVDPVGACIGARGSRIQQVVNELRGEKIDVIRWSPDPGQYIANSL
SPARVEMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDIKNSQEYDQASEDTTVAELISQREEEEALQ
RDAESRLAAEQATRAEEDARLRELYPLPEDEEEYDQEEPAKTMAEDENASDADGQPDDLSSQPDTSSEQLSNEESVEEED
RAR

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=363, Percent_Identity=33.3333333333333, Blast_Score=185, Evalue=5e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54373; Mature: 54242

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVLLPGLNNLIEDISEEKKLPTQVVEAALREALLKGYERYRRTLYLGISEDPFEEEYF
CEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHH
SNFDVGLELDDEGYRVLASKIIVEEVESEDHQIALQEVMQVAEDAQIGDTVVLDVTPEKE
CCCCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
DFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTARVIRFERHSVIMAVSSGLGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCC
PEVEAELPRRDQLPNDNYRANATFKVFLKEVSEVPRRGPQLFVSRSNAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCC
EIQEGSVRIVAVAREANPPSRSVGPRTKVAVDSIEREVDPVGACIGARGSRIQQVVNELR
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIRWSPDPGQYIANSLSPARVEMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCCHHHHHHCCCHHHEEEEEEECCCCCEEEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDIKNSQEYDQASEDTTVAELISQREEEEALQRDAESRLAAEQATRAEEDA
EEEEEECEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLRELYPLPEDEEEYDQEEPAKTMAEDENASDADGQPDDLSSQPDTSSEQLSNEESVEEE
HHHHHCCCCCCHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHH
DRAR
HCCC
>Mature Secondary Structure 
ALVLLPGLNNLIEDISEEKKLPTQVVEAALREALLKGYERYRRTLYLGISEDPFEEEYF
EEEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHH
SNFDVGLELDDEGYRVLASKIIVEEVESEDHQIALQEVMQVAEDAQIGDTVVLDVTPEKE
CCCCCCEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
DFGRMAAATTKQVLAQKLRDQQRRMIQEEFADLEDPVLTARVIRFERHSVIMAVSSGLGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEECCCCCC
PEVEAELPRRDQLPNDNYRANATFKVFLKEVSEVPRRGPQLFVSRSNAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEECCCCC
EIQEGSVRIVAVAREANPPSRSVGPRTKVAVDSIEREVDPVGACIGARGSRIQQVVNELR
CCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIRWSPDPGQYIANSLSPARVEMVRLVDPEGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCCHHHHHHCCCHHHEEEEEEECCCCCEEEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDIKNSQEYDQASEDTTVAELISQREEEEALQRDAESRLAAEQATRAEEDA
EEEEEECEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RLRELYPLPEDEEEYDQEEPAKTMAEDENASDADGQPDDLSSQPDTSSEQLSNEESVEEE
HHHHHCCCCCCHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCHH
DRAR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]