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The map label for this gene is dnaN [H]

Identifier: 124021715

GI number: 124021715

Start: 205

End: 1371

Strand: Direct

Name: dnaN [H]

Synonym: P9303_00001

Alternate gene names: 124021715

Gene position: 205-1371 (Clockwise)

Preceding gene: NA

Following gene: 124021716

Centisome position: 0.01

GC content: 44.9

Gene sequence:

>1167_bases
ATGAAACTGGTTTGTTCACAGGCTGAACTCAATACAGCACTACAGCTCGTTAGCAGAGCCGTGGCCTCACGACCCACTCA
TCCGGTGCTAGCTAACGTGCTTCTCACTGCTGATGCTGGCACTGATCGTCTCAGCCTCACGGGTTTTGATCTGAGCCTTG
GTATTCAAACGGCATTGACTGCCTCGGTGGAATCCAGTGGTGCGATTACTTTGCCTGCGCGCCTATTAGGGGAAATTGTT
TCAAGGCTTTCAAGTGATTCGCCTATCACTTTGGCCACTGATGAGGCTGGTGAACAGGTAGAGCTCAAAAGTTTAAGCGG
GAGTTATCAGATGCGTGGGATGTCTGCTGATGATTTTCCTGAATTACCTCTTGTTGAAAGTGGTAAGGCTGTCAAGGTGA
ATGCGAGGGCTCTTTTGACAGCTCTTCGAGGAACTCTTTTTGCTAGCAGTTCAGATGAAGCCAAACAGTTACTAACAGGA
GTTCATTTGCATTTTGATGGTAAAGCGATGGAAGCAGCGGCAACTGATGGTCATCGCCTTGCAGTTTTAAGCTTGGCAGA
TGCCTTAGATGTTGAGACTACGCTTTCATCTGACACGGATGATGAAGGTGAGAATTTTGCGGTAACGCTTCCTTCTCGTT
CTTTGCGGGAAGTGGAGCGCTTGATCGCCGGATGGCGTAGTGATGATCAAGTGAGTTTGTTTTGTGATAAAGGTCAGGTG
GTATTTTTAGCTGCTGATCAAGTTGTAACTAGTCGAACATTAGACGGAACTTATCCTAACTATCGTCAGTTAATTCCTGA
TGGTTTTGCTCGGAGCTTTGATGTTGATCGGCGTGCTTTTATTTCAGCTTTGGAACGGATAGCTGTATTAGCTGATCAAC
ATAACAATGTTGTAAAGGTTAGTGGTGACAGTACTTCTGAGCTTCTACAAATTAGTGCTGATGCTCAGGATGTAGGCAGT
GGCTCTGAATCACTATCGGCTGAATTTACTGGTGATTCTGTTCAGATTGCTTTTAATGTTCGTTATGTACTAGATGGTTT
GAAGGTGATGGATAGTGATCGTATTGTACTGCGTTGTAATGCTCCAACCACACCTGCAATTATTTCACCAAAGGATGATG
ATATTGGCTTTACGTATTTGGTGATGCCAGTACAAATTCGTTCTTGA

Upstream 100 bases:

>100_bases
GGGTTGTGAATGAGAATGATCTGCCATTGCACTTCTTGCTGGCTTGTGAAATTCTGGGAGACCTCTTCAGCGTTCTCGTT
CTAGTCGTGACATTGATTCC

Downstream 100 bases:

>100_bases
ACTTTGAATCTTCCTGATCAGATTTTATTGAGTGATCTGTTGCATCATCGTGTACGTTGTGATCAGGGATTAGATCATGG
GCCTGGAGTAATGCCTTGGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKLVCSQAELNTALQLVSRAVASRPTHPVLANVLLTADAGTDRLSLTGFDLSLGIQTALTASVESSGAITLPARLLGEIV
SRLSSDSPITLATDEAGEQVELKSLSGSYQMRGMSADDFPELPLVESGKAVKVNARALLTALRGTLFASSSDEAKQLLTG
VHLHFDGKAMEAAATDGHRLAVLSLADALDVETTLSSDTDDEGENFAVTLPSRSLREVERLIAGWRSDDQVSLFCDKGQV
VFLAADQVVTSRTLDGTYPNYRQLIPDGFARSFDVDRRAFISALERIAVLADQHNNVVKVSGDSTSELLQISADAQDVGS
GSESLSAEFTGDSVQIAFNVRYVLDGLKVMDSDRIVLRCNAPTTPAIISPKDDDIGFTYLVMPVQIRS

Sequences:

>Translated_388_residues
MKLVCSQAELNTALQLVSRAVASRPTHPVLANVLLTADAGTDRLSLTGFDLSLGIQTALTASVESSGAITLPARLLGEIV
SRLSSDSPITLATDEAGEQVELKSLSGSYQMRGMSADDFPELPLVESGKAVKVNARALLTALRGTLFASSSDEAKQLLTG
VHLHFDGKAMEAAATDGHRLAVLSLADALDVETTLSSDTDDEGENFAVTLPSRSLREVERLIAGWRSDDQVSLFCDKGQV
VFLAADQVVTSRTLDGTYPNYRQLIPDGFARSFDVDRRAFISALERIAVLADQHNNVVKVSGDSTSELLQISADAQDVGS
GSESLSAEFTGDSVQIAFNVRYVLDGLKVMDSDRIVLRCNAPTTPAIISPKDDDIGFTYLVMPVQIRS
>Mature_388_residues
MKLVCSQAELNTALQLVSRAVASRPTHPVLANVLLTADAGTDRLSLTGFDLSLGIQTALTASVESSGAITLPARLLGEIV
SRLSSDSPITLATDEAGEQVELKSLSGSYQMRGMSADDFPELPLVESGKAVKVNARALLTALRGTLFASSSDEAKQLLTG
VHLHFDGKAMEAAATDGHRLAVLSLADALDVETTLSSDTDDEGENFAVTLPSRSLREVERLIAGWRSDDQVSLFCDKGQV
VFLAADQVVTSRTLDGTYPNYRQLIPDGFARSFDVDRRAFISALERIAVLADQHNNVVKVSGDSTSELLQISADAQDVGS
GSESLSAEFTGDSVQIAFNVRYVLDGLKVMDSDRIVLRCNAPTTPAIISPKDDDIGFTYLVMPVQIRS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=391, Percent_Identity=26.5984654731458, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41421; Mature: 41421

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVCSQAELNTALQLVSRAVASRPTHPVLANVLLTADAGTDRLSLTGFDLSLGIQTALT
CCEEECHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEECCCCCEEEEEEEEEEECHHHHEE
ASVESSGAITLPARLLGEIVSRLSSDSPITLATDEAGEQVELKSLSGSYQMRGMSADDFP
EECCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEECCCCCCCCC
ELPLVESGKAVKVNARALLTALRGTLFASSSDEAKQLLTGVHLHFDGKAMEAAATDGHRL
CCCEECCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCEEEHCCCCCCEE
AVLSLADALDVETTLSSDTDDEGENFAVTLPSRSLREVERLIAGWRSDDQVSLFCDKGQV
EEEEHHHHHCCHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCE
VFLAADQVVTSRTLDGTYPNYRQLIPDGFARSFDVDRRAFISALERIAVLADQHNNVVKV
EEEECCCEEECCCCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
SGDSTSELLQISADAQDVGSGSESLSAEFTGDSVQIAFNVRYVLDGLKVMDSDRIVLRCN
CCCCCCCEEEEECCHHHCCCCCCCCEEEECCCEEEEEEEEEEECCCCEEECCCCEEEEEC
APTTPAIISPKDDDIGFTYLVMPVQIRS
CCCCCCEECCCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MKLVCSQAELNTALQLVSRAVASRPTHPVLANVLLTADAGTDRLSLTGFDLSLGIQTALT
CCEEECHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEECCCCCEEEEEEEEEEECHHHHEE
ASVESSGAITLPARLLGEIVSRLSSDSPITLATDEAGEQVELKSLSGSYQMRGMSADDFP
EECCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCEEECCCCCCCCC
ELPLVESGKAVKVNARALLTALRGTLFASSSDEAKQLLTGVHLHFDGKAMEAAATDGHRL
CCCEECCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHEEEEEECCCEEEHCCCCCCEE
AVLSLADALDVETTLSSDTDDEGENFAVTLPSRSLREVERLIAGWRSDDQVSLFCDKGQV
EEEEHHHHHCCHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCE
VFLAADQVVTSRTLDGTYPNYRQLIPDGFARSFDVDRRAFISALERIAVLADQHNNVVKV
EEEECCCEEECCCCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
SGDSTSELLQISADAQDVGSGSESLSAEFTGDSVQIAFNVRYVLDGLKVMDSDRIVLRCN
CCCCCCCEEEEECCHHHCCCCCCCCEEEECCCEEEEEEEEEEECCCCEEECCCCEEEEEC
APTTPAIISPKDDDIGFTYLVMPVQIRS
CCCCCCEECCCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8654968 [H]