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The map label for this gene is pntB [H]

Identifier: 123968853

GI number: 123968853

Start: 1098493

End: 1099932

Strand: Reverse

Name: pntB [H]

Synonym: A9601_13201

Alternate gene names: 123968853

Gene position: 1099932-1098493 (Counterclockwise)

Preceding gene: 123968854

Following gene: 123968852

Centisome position: 65.87

GC content: 37.78

Gene sequence:

>1440_bases
ATGAATCTACCTGTAATCATTAAATTCGTTATTGACCTTCTAGCAGTACTTTTACTGGCTTTGGGAATAAAAGGTTTGTC
AAAAGTAAAATCAGCAAGGGATGCCAATAGATTAGCTGCATTTGCAATGTCGCTATCAGTAGTAGGATTACTTTCTTACT
ATTTAGGCACTTCTGGAATTGCTATTCAGTCTTGGATTTGGATAATAATTGGATCAATCATAGGTAGTTTATTCGGAGCA
ATTCTTGCAAAAAAAGTACCTATGACCTCCATGCCTGAAACGGTGGCATTGTTCAATGGTTGTGGAGGAATGTCATCTCT
TTTAGTGGCCTTAGGAGTAGCTATTTTTCCTATATCTGGTGGCCAAGAAAATTTTGATTTTTTTAAGTCACTGATTAACG
AAGTTTCAATATCTGTTTCTATATTTGTTGGTGCCATAACTTTCACAGGTTCAATTGTTGCGATGGCAAAGTTACAGGGG
TGGTTGTCAACTCCAGGATGGACTCAGAGTAAAGTTAGGCATTTTGTAAATATTGTTTTTGCAGTTGCTTCCTTGATAGC
CTTTTTTGATTTGATAAACGGCAACACAAGTTCTATTTGGCTTTTAGTTATAGTTTCTTCTTTATTAGGCATTGGAGTTA
CTTTGCCAATTGGTGGAGCTGATATGCCAGTCGTTATATCTTTATTAAATAGCTATTCAGGGATTGCAGCAGCAGCGGCA
GGTTTCGTTGTTGATAGTCAGCTTTTGATAGTAGCAGGCGCAATGGTTGGAGCAGCAGGTCTAATACTTACACAAGTTAT
GTGCAAGGGTATGAATAGATCATTGGTCTCAGTTCTTTTTGGAGGATCTTTAGCTGCACAAAGTACAGCTTCTTCTGGTT
CTGGAGAATATACAAATATAACTTCTTGCAGTGTTGAAGAATGTGCATTGACTTTAGAGGCAGCCAACAAGGTAATTATT
GTTCCTGGGTATGGTCTAGCGGTAGCTCAAGCTCAACATACTTTAAGGGAAGTGACTAAAAAACTAGAGCAAAATGGTAT
TGAAGTTGTTTACGCAATTCATCCTGTAGCAGGGAGGATGCCTGGACATATGAATGTACTTTTAGCAGAAGCAGATGTTC
CTTACGAACAACTTAAAGAGATGGACGTTGTAAATCCTGATTTTCCAGCAACGGATGTTGTTTTAGTTTTAGGAGCAAAT
GATGTGGTTAATCCTCAAGCTAAAAATGATAGCTCTTCTCCTTTATATGGCATGCCAGTACTTGACGTGCAGGAAGCAAG
AACAGTATTTGTAATTAAACGTGGTATGAGTGCAGGTTACTCTGGAATAAAAAATGATTTATTTGATCTGCCAAATACCT
CTATGGTATTTGGTGATGCAAAAAAGGTACTCAATGATTTGATTGGGGAATTAAAGGATCTTGGAGTTGGGGAGAAATAA

Upstream 100 bases:

>100_bases
TTGGATTTGCTCTTTTCAACGTTGTAGGTGGTTTCTTTGTAACTGATCGAATGCTCGCGATGTTTAGTCGTAAACCATCA
AATAAGAAGTAATTTTTAAC

Downstream 100 bases:

>100_bases
TAGTATATCTTTTAGTTTTTCAGATTACTTAAAAAATAAGCCATTTCGAGAAGTCTTACCTTGGATAGGTGGCGACTTAC
AAACTTTGAGAGATACGTTT

Product: putative nicotinamide nucleotide transhydrogenase, subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGIAIQSWIWIIIGSIIGSLFGA
ILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISGGQENFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQG
WLSTPGWTQSKVRHFVNIVFAVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLAAQSTASSGSGEYTNITSCSVEECALTLEAANKVII
VPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGAN
DVVNPQAKNDSSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGEK

Sequences:

>Translated_479_residues
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGIAIQSWIWIIIGSIIGSLFGA
ILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISGGQENFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQG
WLSTPGWTQSKVRHFVNIVFAVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLAAQSTASSGSGEYTNITSCSVEECALTLEAANKVII
VPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGAN
DVVNPQAKNDSSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGEK
>Mature_479_residues
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGIAIQSWIWIIIGSIIGSLFGA
ILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISGGQENFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQG
WLSTPGWTQSKVRHFVNIVFAVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLAAQSTASSGSGEYTNITSCSVEECALTLEAANKVII
VPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGAN
DVVNPQAKNDSSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGEK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=414, Percent_Identity=40.5797101449275, Blast_Score=285, Evalue=6e-77,
Organism=Homo sapiens, GI122939153, Length=414, Percent_Identity=40.5797101449275, Blast_Score=285, Evalue=6e-77,
Organism=Escherichia coli, GI1787886, Length=466, Percent_Identity=42.4892703862661, Blast_Score=343, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI17550456, Length=414, Percent_Identity=42.512077294686, Blast_Score=302, Evalue=2e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 50162; Mature: 50162

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGI
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AIQSWIWIIIGSIIGSLFGAILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHEECCC
GQENFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQGWLSTPGWTQSKVRHFVNIVF
CCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCHHHHHHH
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLAAQSTASSGSGEYTNI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCCCCCCCCCCCC
TSCSVEECALTLEAANKVIIVPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRM
CCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCC
PGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGANDVVNPQAKNDSSSPLYGMPV
CCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEECCCC
LDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGEK
CCHHHCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGI
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AIQSWIWIIIGSIIGSLFGAILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHEECCC
GQENFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQGWLSTPGWTQSKVRHFVNIVF
CCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
AVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCHHHHHHH
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLAAQSTASSGSGEYTNI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCCCCCCCCCCCC
TSCSVEECALTLEAANKVIIVPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRM
CCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCC
PGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGANDVVNPQAKNDSSSPLYGMPV
CCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEECCCC
LDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGEK
CCHHHCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]