Click here to switch to the map view.

The map label for this gene is sdhA [C]

Identifier: 123968792

GI number: 123968792

Start: 1064207

End: 1065961

Strand: Reverse

Name: sdhA [C]

Synonym: A9601_12591

Alternate gene names: 123968792

Gene position: 1065961-1064207 (Counterclockwise)

Preceding gene: 123968794

Following gene: 123968791

Centisome position: 63.83

GC content: 33.96

Gene sequence:

>1755_bases
TTGCCTGTTAAAGAACATCTCACTCATATATCAAATGTTCTCGTGATTGGGTGTGGGGGAGCTGGTCTAAGATCTGCTAT
TGAAATTAAAAAATCTGGTCTCAATGTATGTATCCTAGGGAAAAGACCGAAGACAGATGCTCATACCGTTTTAGCAGCAG
GAGGTATTAATGCTGCTTTAGGCAACCTAGATAAAGAAGACACATGGGAACAGCATTTTGTTGATACTTATTTAGAGGGG
TATGGTATTGGTGATCCTTTAAAAGTTGAAATAATGGCTAAAGAATCCCCATTTTTAGTAAAAGAAATAGATAAATGGGG
GGCTAACTTTGCAAAATTAAAAAATGGTGAACTCGATCAAAGATTTTTTGGTGCACATAAATATCGTAGAACTTGTTATT
CAGGTGATTTTACTGGATTATCAATTTTAAAAACTCTCTTAAAAAAATCAGATGATTTAAAGATTCCTATTTATGACAAT
CAATATGTTACTGAATTATTGATTAGGGAAAATACCTGCTTTGGAGCAATGTCGTTTAACTTGTCTACTTCAGAGAGGAC
TGTGCACTTAGCAGATGCAGTTATTTTATGTACGGGAGGGCATACAAGATTATGGAAAAAAAGTTCATCTAGAAAAAATG
AAAATACAGGTGATGGTTATTATTTAAGTCTAAAAGGAGGATGTGAATTAATAGATATGGAAATGGTACAATTCCACCCT
TCTGGAATGGTCTTACCCGAGGAAATTGAAGGAACTTTGGTTACTGAAGCTGTAAGAGGTGAGGGTGGAAAGTTACTAAA
TAATAAAGGTGAAAGATTTATGAAAGAATATGATCCGAAAAGGATGGAATTATCAACAAGAGATAGAGTAGCAATTGCTA
ACTATACAGAAATAATTGAAGGCAGAGGAACAAAAAATGGGGGTGTTTTTCTTGATATAAGCCATAAAAGTAAAGAGTTT
ATTGTTGAAAAGCTGCCAAGCATATATAGACAATTTCTTGAAACCCAAATGCTTGATATTTCAAAGTCGCCAATGGAAGT
TGCACCAACTGCTCACTATTCTATGGGTGGAATTTTAGTTAATCCAGAGAATTTATCTACTTCTGTAGAAGGTCTTTTTG
CCGCTGGTGAAGTTGCAGGAGGACTTCACGGAGCTAATCGATTGGGTGGTAATTCTTTGGCAGAAATTATTATTTTTGGA
AGACGTGCGGGCATTGCAGCTTCAAAATATTCAAAAAATAAAGATCATCAAATGAGATCAAATAAAGCCATCGCCATTGC
ACATGAAAACATTAATAAGTTTATTAAAAATGGTAATGAATTAGTGGGGCCTCTTCAAAATGAATTGCGATTAATAATGT
GGAAATATTGTGGGGTTATTAAAAATGAGACATTACTTTTAGAGGGGTTATCAAAAATTGAAAGTATAAAAAGAAAACTC
AGTGACGTTGATGTAAGAATCGATCAATATAATTGCGAAGATCTAGCATTGATTTTTGATTTGCAATCTTCTTTGTTGAG
TGCAAAAGCGTCAATTGTTTCGGCATTACAAAGAAATGAGAGTAGGGGCGCACATCAAAGAAGTGATTTCCCATTACTAG
ACCCCTCATTTCAATTTAATTGCGCAGTAAGGTTGGATGATGATAATAATTTAAAAATTTATAAAGTACCTATAAAGGAA
CTAAATAACCAACAAAAAACAATTGTTGCAAATGCAAAAAGAGAAGAGGATATCAGAAATAAACTGCTTGAGTAA

Upstream 100 bases:

>100_bases
CCGACAATTTTAGAAACATTTGACCACATTTAAAATAATGAAATTATTTATATCTTTCTAAGGCATAGAGTTAAAATTAA
ATTATTAAATTTACTTAAAA

Downstream 100 bases:

>100_bases
TGTGATTATTTAATTCTAAATTAAAGGATTAATTACGTTAATTATAAAAAATTTAAATGAATAAAAAATAAATTCTTAAC
CACTATTTGACCTCAATTTG

Product: succinate dehydrogenase

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MPVKEHLTHISNVLVIGCGGAGLRSAIEIKKSGLNVCILGKRPKTDAHTVLAAGGINAALGNLDKEDTWEQHFVDTYLEG
YGIGDPLKVEIMAKESPFLVKEIDKWGANFAKLKNGELDQRFFGAHKYRRTCYSGDFTGLSILKTLLKKSDDLKIPIYDN
QYVTELLIRENTCFGAMSFNLSTSERTVHLADAVILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHP
SGMVLPEEIEGTLVTEAVRGEGGKLLNNKGERFMKEYDPKRMELSTRDRVAIANYTEIIEGRGTKNGGVFLDISHKSKEF
IVEKLPSIYRQFLETQMLDISKSPMEVAPTAHYSMGGILVNPENLSTSVEGLFAAGEVAGGLHGANRLGGNSLAEIIIFG
RRAGIAASKYSKNKDHQMRSNKAIAIAHENINKFIKNGNELVGPLQNELRLIMWKYCGVIKNETLLLEGLSKIESIKRKL
SDVDVRIDQYNCEDLALIFDLQSSLLSAKASIVSALQRNESRGAHQRSDFPLLDPSFQFNCAVRLDDDNNLKIYKVPIKE
LNNQQKTIVANAKREEDIRNKLLE

Sequences:

>Translated_584_residues
MPVKEHLTHISNVLVIGCGGAGLRSAIEIKKSGLNVCILGKRPKTDAHTVLAAGGINAALGNLDKEDTWEQHFVDTYLEG
YGIGDPLKVEIMAKESPFLVKEIDKWGANFAKLKNGELDQRFFGAHKYRRTCYSGDFTGLSILKTLLKKSDDLKIPIYDN
QYVTELLIRENTCFGAMSFNLSTSERTVHLADAVILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHP
SGMVLPEEIEGTLVTEAVRGEGGKLLNNKGERFMKEYDPKRMELSTRDRVAIANYTEIIEGRGTKNGGVFLDISHKSKEF
IVEKLPSIYRQFLETQMLDISKSPMEVAPTAHYSMGGILVNPENLSTSVEGLFAAGEVAGGLHGANRLGGNSLAEIIIFG
RRAGIAASKYSKNKDHQMRSNKAIAIAHENINKFIKNGNELVGPLQNELRLIMWKYCGVIKNETLLLEGLSKIESIKRKL
SDVDVRIDQYNCEDLALIFDLQSSLLSAKASIVSALQRNESRGAHQRSDFPLLDPSFQFNCAVRLDDDNNLKIYKVPIKE
LNNQQKTIVANAKREEDIRNKLLE
>Mature_583_residues
PVKEHLTHISNVLVIGCGGAGLRSAIEIKKSGLNVCILGKRPKTDAHTVLAAGGINAALGNLDKEDTWEQHFVDTYLEGY
GIGDPLKVEIMAKESPFLVKEIDKWGANFAKLKNGELDQRFFGAHKYRRTCYSGDFTGLSILKTLLKKSDDLKIPIYDNQ
YVTELLIRENTCFGAMSFNLSTSERTVHLADAVILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHPS
GMVLPEEIEGTLVTEAVRGEGGKLLNNKGERFMKEYDPKRMELSTRDRVAIANYTEIIEGRGTKNGGVFLDISHKSKEFI
VEKLPSIYRQFLETQMLDISKSPMEVAPTAHYSMGGILVNPENLSTSVEGLFAAGEVAGGLHGANRLGGNSLAEIIIFGR
RAGIAASKYSKNKDHQMRSNKAIAIAHENINKFIKNGNELVGPLQNELRLIMWKYCGVIKNETLLLEGLSKIESIKRKLS
DVDVRIDQYNCEDLALIFDLQSSLLSAKASIVSALQRNESRGAHQRSDFPLLDPSFQFNCAVRLDDDNNLKIYKVPIKEL
NNQQKTIVANAKREEDIRNKLLE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=569, Percent_Identity=32.8646748681898, Blast_Score=258, Evalue=1e-68,
Organism=Escherichia coli, GI1786942, Length=561, Percent_Identity=35.4723707664884, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1790597, Length=519, Percent_Identity=33.3333333333333, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788928, Length=542, Percent_Identity=31.3653136531365, Blast_Score=234, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17505833, Length=531, Percent_Identity=33.5216572504708, Blast_Score=278, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI17550100, Length=542, Percent_Identity=34.1328413284133, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI71986328, Length=479, Percent_Identity=26.9311064718163, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=556, Percent_Identity=35.2517985611511, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322416, Length=565, Percent_Identity=34.8672566371681, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6322511, Length=471, Percent_Identity=29.2993630573248, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320788, Length=477, Percent_Identity=29.559748427673, Blast_Score=124, Evalue=5e-29,
Organism=Drosophila melanogaster, GI17137288, Length=560, Percent_Identity=33.0357142857143, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24655642, Length=560, Percent_Identity=33.0357142857143, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24655647, Length=560, Percent_Identity=33.0357142857143, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24663005, Length=553, Percent_Identity=34.5388788426763, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 64959; Mature: 64828

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVKEHLTHISNVLVIGCGGAGLRSAIEIKKSGLNVCILGKRPKTDAHTVLAAGGINAAL
CCHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHH
GNLDKEDTWEQHFVDTYLEGYGIGDPLKVEIMAKESPFLVKEIDKWGANFAKLKNGELDQ
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHH
RFFGAHKYRRTCYSGDFTGLSILKTLLKKSDDLKIPIYDNQYVTELLIRENTCFGAMSFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEEE
LSTSERTVHLADAVILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHP
CCCCCCEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEECC
SGMVLPEEIEGTLVTEAVRGEGGKLLNNKGERFMKEYDPKRMELSTRDRVAIANYTEIIE
CCCCCHHHCCCEEEEHHHCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCEEECCHHHHHC
GRGTKNGGVFLDISHKSKEFIVEKLPSIYRQFLETQMLDISKSPMEVAPTAHYSMGGILV
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCEEE
NPENLSTSVEGLFAAGEVAGGLHGANRLGGNSLAEIIIFGRRAGIAASKYSKNKDHQMRS
CCCCCCCHHHHHEEHHHHHCCCCCCCCCCCCHHEEEEEEECCCCCCHHHCCCCCCHHHHC
NKAIAIAHENINKFIKNGNELVGPLQNELRLIMWKYCGVIKNETLLLEGLSKIESIKRKL
CCEEEEEHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHH
SDVDVRIDQYNCEDLALIFDLQSSLLSAKASIVSALQRNESRGAHQRSDFPLLDPSFQFN
HHCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
CAVRLDDDNNLKIYKVPIKELNNQQKTIVANAKREEDIRNKLLE
EEEEECCCCCEEEEEECHHHCCCCCEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure 
PVKEHLTHISNVLVIGCGGAGLRSAIEIKKSGLNVCILGKRPKTDAHTVLAAGGINAAL
CHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHH
GNLDKEDTWEQHFVDTYLEGYGIGDPLKVEIMAKESPFLVKEIDKWGANFAKLKNGELDQ
CCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHH
RFFGAHKYRRTCYSGDFTGLSILKTLLKKSDDLKIPIYDNQYVTELLIRENTCFGAMSFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEEE
LSTSERTVHLADAVILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHP
CCCCCCEEEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEECCCCEEEEEEEEEECC
SGMVLPEEIEGTLVTEAVRGEGGKLLNNKGERFMKEYDPKRMELSTRDRVAIANYTEIIE
CCCCCHHHCCCEEEEHHHCCCCCCCCCCCHHHHHHHCCCCCEEECCCCCEEECCHHHHHC
GRGTKNGGVFLDISHKSKEFIVEKLPSIYRQFLETQMLDISKSPMEVAPTAHYSMGGILV
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCEEE
NPENLSTSVEGLFAAGEVAGGLHGANRLGGNSLAEIIIFGRRAGIAASKYSKNKDHQMRS
CCCCCCCHHHHHEEHHHHHCCCCCCCCCCCCHHEEEEEEECCCCCCHHHCCCCCCHHHHC
NKAIAIAHENINKFIKNGNELVGPLQNELRLIMWKYCGVIKNETLLLEGLSKIESIKRKL
CCEEEEEHHHHHHHHHCCCCEECCHHHHHHHHHHHHHCHHCCCCHHHHHHHHHHHHHHHH
SDVDVRIDQYNCEDLALIFDLQSSLLSAKASIVSALQRNESRGAHQRSDFPLLDPSFQFN
HHCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEE
CAVRLDDDNNLKIYKVPIKELNNQQKTIVANAKREEDIRNKLLE
EEEEECCCCCEEEEEECHHHCCCCCEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.4 {fumarate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]