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The map label for this gene is cobB [H]

Identifier: 123968710

GI number: 123968710

Start: 991581

End: 992972

Strand: Reverse

Name: cobB [H]

Synonym: A9601_11771

Alternate gene names: 123968710

Gene position: 992972-991581 (Counterclockwise)

Preceding gene: 123968712

Following gene: 123968706

Centisome position: 59.46

GC content: 31.18

Gene sequence:

>1392_bases
ATGCCTTGTGTAATATCTTCTCCTTCAACTGATAGCGGGAAAACTACATTATCTCTTTTGCTATCTTGTTGGGCGTTCTC
AAAAGGTATAAAGATACAAACTTTCAAGGTGGGCCCAGATTATCTTGATCAACAACAACTTAGCTCAATTGGTCAACCTA
TTTGTAGGAATTTAGATATTTTTTTAAGTGGTGAGGAATGGGTTCAAGAAAGTTTTTTTAAACATTCTTTGAAATATGAA
TTCTCGTTAATTGAAGGAGCAATGGGTCTGTTTGATGGACTAGGGTCAACAACCTATTCCAGCACAGCAAATATCTCTAA
ACTTCTCAATGCTCCAATAGTTTTTATTGTTAATGCTAGAGGTCAAGTAGCTTCTCTTTTGGCGACTATTCGAGGTTTTA
GAGATTTCGATAGTGAGTTGTCAATAGTAGGAATTATATTTAACAACGTTAATTCAGATCGACATAAAAAATTAATCAAA
GAAGTTTTTAAAAATGAAGATATCGAAATTCTGGGTTTTTTACCATCTGATTCAAAAATAACTTTAAACAAAGCTAATTT
AGGTTTGATATCTCCAATGGATAATGGAAAAGAAATTGATGTTGAATATTTTGCAAATTTCGCCGAAAGAAATCTTGATG
TATTTTCTCTTATTAAATTTCTGAAATCTCCTCAAAAGAAAAAATTTAATTCTTTCAATTTTAAAGATTTTAAAATAGAC
AAAAGTAAACCTATCGCAATTGCAGAAGATAAAATCTTTCATTTTCAATATCCTGAAACTAAGGAGTTTTTGAGTGAACT
AGGAATACCATTGATTTCATGGAGTATTTATGATGATGAAGAAATACCTAATGAGGCTTCTTCTTTAATTATTCCTGGGG
GGTTCCCTGAAAAATATGCTGATCATATAAGTAACTCTACAAAAAGCTTAAATTCGTTAAGGAAATTCCGCAAAAATGGA
TTTATATATGCCGAGTGTGGAGGGATGATGATTTTAGGTGACTTCATAAAAGATGAAAATGGTAATTATCATAAAATGAG
TGGCATCCTGCCTTTTAGATCAAAAAAAAGTAAACTTACAGTGGGTTATAGATACATTAAGGGTTTAAAAGATACTCCGA
TCATTAAACAAAATCAATTCATAAGAGGACATGAATTTCATTATTGGGAAATTGAAAATAATTTGTCTGAACTTGATTTA
AGAAAAGCTGAGTATCATAAGAAACTATTTTCCCCGTGGAAAATCAAATCTTGGGAAACTGAATATAAAAATGAAGGCTA
TTTTGATGAAAAATTACATGCAAGTTGGATTCACTTACATTTGCCAAGTTCTCCAGAAGTCGCAAAAAACTTTATAGATG
CCACCCAAATTAGTTTTCCAAAGGATTACTAG

Upstream 100 bases:

>100_bases
TAGTAAATTATTGGGAAGTAGTAGAGGTAATACCAGTCCTCTTTTTGAAAATTCTATTAAAATAGCTCTTCAAATATTTA
ATGGTTTGAATAACTAATAG

Downstream 100 bases:

>100_bases
TTTATTATCAAATCAAAAATTGTGAGAGGAGAACCTAAAAAAAACATTCCAGGCAAAGTAACTAAAGGTCCTAAAAAACT
TCCCACCATGACCTCAATTT

Product: putative cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPCVISSPSTDSGKTTLSLLLSCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLSGEEWVQESFFKHSLKYE
FSLIEGAMGLFDGLGSTTYSSTANISKLLNAPIVFIVNARGQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIK
EVFKNEDIEILGFLPSDSKITLNKANLGLISPMDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKKFNSFNFKDFKID
KSKPIAIAEDKIFHFQYPETKEFLSELGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYADHISNSTKSLNSLRKFRKNG
FIYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKSKLTVGYRYIKGLKDTPIIKQNQFIRGHEFHYWEIENNLSELDL
RKAEYHKKLFSPWKIKSWETEYKNEGYFDEKLHASWIHLHLPSSPEVAKNFIDATQISFPKDY

Sequences:

>Translated_463_residues
MPCVISSPSTDSGKTTLSLLLSCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLSGEEWVQESFFKHSLKYE
FSLIEGAMGLFDGLGSTTYSSTANISKLLNAPIVFIVNARGQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIK
EVFKNEDIEILGFLPSDSKITLNKANLGLISPMDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKKFNSFNFKDFKID
KSKPIAIAEDKIFHFQYPETKEFLSELGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYADHISNSTKSLNSLRKFRKNG
FIYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKSKLTVGYRYIKGLKDTPIIKQNQFIRGHEFHYWEIENNLSELDL
RKAEYHKKLFSPWKIKSWETEYKNEGYFDEKLHASWIHLHLPSSPEVAKNFIDATQISFPKDY
>Mature_462_residues
PCVISSPSTDSGKTTLSLLLSCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLSGEEWVQESFFKHSLKYEF
SLIEGAMGLFDGLGSTTYSSTANISKLLNAPIVFIVNARGQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIKE
VFKNEDIEILGFLPSDSKITLNKANLGLISPMDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKKFNSFNFKDFKIDK
SKPIAIAEDKIFHFQYPETKEFLSELGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYADHISNSTKSLNSLRKFRKNGF
IYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKSKLTVGYRYIKGLKDTPIIKQNQFIRGHEFHYWEIENNLSELDLR
KAEYHKKLFSPWKIKSWETEYKNEGYFDEKLHASWIHLHLPSSPEVAKNFIDATQISFPKDY

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 52937; Mature: 52806

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCVISSPSTDSGKTTLSLLLSCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDI
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEE
FLSGEEWVQESFFKHSLKYEFSLIEGAMGLFDGLGSTTYSSTANISKLLNAPIVFIVNAR
EECCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECC
GQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIKEVFKNEDIEILGFLPSDSKI
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEE
TLNKANLGLISPMDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKKFNSFNFKDFKID
EEEECCCEEECCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCEEEEEC
KSKPIAIAEDKIFHFQYPETKEFLSELGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYA
CCCCEEEECCCEEEECCCCHHHHHHHCCCCEEEEEECCCHHCCCCCCCEEECCCCCHHHH
DHISNSTKSLNSLRKFRKNGFIYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKSKLT
HHHCCHHHHHHHHHHHHHCCEEEEECCCEEEEEEHHHCCCCCEEEECCCCCCCCCCCEEE
VGYRYIKGLKDTPIIKQNQFIRGHEFHYWEIENNLSELDLRKAEYHKKLFSPWKIKSWET
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCC
EYKNEGYFDEKLHASWIHLHLPSSPEVAKNFIDATQISFPKDY
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PCVISSPSTDSGKTTLSLLLSCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDI
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEE
FLSGEEWVQESFFKHSLKYEFSLIEGAMGLFDGLGSTTYSSTANISKLLNAPIVFIVNAR
EECCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECC
GQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIKEVFKNEDIEILGFLPSDSKI
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEE
TLNKANLGLISPMDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKKFNSFNFKDFKID
EEEECCCEEECCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCEEEEEC
KSKPIAIAEDKIFHFQYPETKEFLSELGIPLISWSIYDDEEIPNEASSLIIPGGFPEKYA
CCCCEEEECCCEEEECCCCHHHHHHHCCCCEEEEEECCCHHCCCCCCCEEECCCCCHHHH
DHISNSTKSLNSLRKFRKNGFIYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKSKLT
HHHCCHHHHHHHHHHHHHCCEEEEECCCEEEEEEHHHCCCCCEEEECCCCCCCCCCCEEE
VGYRYIKGLKDTPIIKQNQFIRGHEFHYWEIENNLSELDLRKAEYHKKLFSPWKIKSWET
EHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCC
EYKNEGYFDEKLHASWIHLHLPSSPEVAKNFIDATQISFPKDY
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA