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The map label for this gene is proS [H]

Identifier: 123968100

GI number: 123968100

Start: 491307

End: 493109

Strand: Reverse

Name: proS [H]

Synonym: A9601_05651

Alternate gene names: 123968100

Gene position: 493109-491307 (Counterclockwise)

Preceding gene: 123968106

Following gene: 123968099

Centisome position: 29.53

GC content: 32.95

Gene sequence:

>1803_bases
ATGCGCGTGACAACCTCATTTCCTCTGGGGACACTTCGTGACACACCTTCTGAAGCTGAGATTATTTCACATCAATTACT
CTTAAAAGCTGGGTATATTCGCAGAGTTAACAGCGGTATTTATGCATACATGCCACTAATGCTTAGAGTTATTGAAAAAA
TATCCGCAATAATAGAGAAAGAACTTAATAGTATTGGTTGTACAAAATTACTATTACCCCAACTTCATCCTGCAGATTTA
TGGAAAAAAAGTGAAAGGTGGGAAGGATATACCGCAGGTGAAGGAATAATGTTTAATCTCAAAGATAGACAAGGCAAGGA
ATTTGGTTTAGCACCAACTCACGAAGAGGTGATCACGAGTATTGCATCAGAGACAATAAACTCCTATAAGCAATTACCTC
AATGTTTTTACCAAATTCAGACAAAATTTAGAGATGAAATAAGGCCAAGGTTTGGATTGATGAGAAGTAGAGAATTCATA
ATGAAAGATGGTTATTCTTTTCATTCTTCACAAAACGATTTAGCTTCTTTCTATGAAAAGGTGAGCAATGCTTATGAAAA
TATTTTTAAATCTTGTGGACTTCAGACAGTAGGGGTTGAAGCTGATAGTGGAGCAATTGGCGGTGCTTCATCTAAAGAAT
TTATGGTCACTGCTGATGCTGGGGAAGATTCCATTTTGTTCACTCAAAGTGGTTCTTATGCTGCCAATATCGAAAAAGCT
GTTTCTCTACCCTCTCAACCTATTCCACTTAAAGATAATATTCCAGAGTGGTTGGAAACACCTCATCAAAAAACAATTCT
AGAAGTTTGCGACAATAACAATTTAGACCCAAGTCAGATTATTAAAGTAGTAATATTCCTTGCACAGTTCGAAGGTGAAT
TTAATGTCCCAATTCTTGCATGCATAAGAGGCGATCAACATATTAATGAAGTAAAGCTTTTTAACTTAATCAATAAACTC
AATCACTTCAATCTCCTTAATCTTAAAAAGATTGAAGACAAAAAAACTATCGAAAAAAATCTAGTTGATTTACCCTTAGG
TTTTATCGGTCCAGATTTAGATAACAATACTATTAAAGCTATTTCTAATTGGGAAAAACAATGGACAAGAATAATTGACC
ATTCTGCAAGTGACCTTTCAAAATTTATAAGTGGTGGGAATAAAGTTAATTTCCATAAAGTTTTTCAAGAATTTTCTTTT
GATTCGAAAGACTATCTGATTGGGGATATCAGGAATGCCAAAAAAGGAGATAAGATAAGTGTTTATGATGATGAAGAACT
TAAAGAAAAAAAAGGTATCGAGATTGGACATATTTTCCAACTAGGTCAGAAATATAGTGAAAAATTAAATGCAAAGTTCT
CTGATAAGGACGGTCAGTTAAAAAATTTATGGATGGGTTGTTACGGAATCGGAGTGACAAGAATAGCTCAAGCTGCTATC
GAACAGAATCATGATCAAAAAGGAATTTGTTGGCCTATCCATATTTCTCCTTTTGAAGTTATTATTATTCCCACAAACCT
AAAAGATCCTATTCAAAGTGATCTTACTGAGCAAATCTATAGCAATTTTTTAATTAATAAAATTGATGTTCTTCTTGATG
ATAGAAACGATAGAGCTGGAGTGAAATTTAAAGATGCGGAATTAATTGGTATTCCTTTTCAGATAATTATTGGCAGAGAT
TCTATTAATAAAGAAGTAGAACTTTTATGCAGAACAAATAATACTAAGCTTAAAATAAGTACTGATAAATTGTTAGAAAC
ATTTATTTCCGAATCTGAAATAATGTACAATAAAAAGTCTTGA

Upstream 100 bases:

>100_bases
AAGAATATTCTTAGACTAGTCGGGTTGAGGGACTCAAGACACTTTAAATTGTTTTTTCATCTTAATTGATAAACTTAGAT
ATTATTAAGGATTTTTCTGT

Downstream 100 bases:

>100_bases
GTCTTATTTTTATTGGGAGCTAAGTTATGTTACTGAAATGGGCATCAAAATTATTTTTTAAGAATCTTACTAAAGCTATA
TCTTTTGCAATATCCTTAAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVIEKISAIIEKELNSIGCTKLLLPQLHPADL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSQNDLASFYEKVSNAYENIFKSCGLQTVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSLPSQPIPLKDNIPEWLETPHQKTILEVCDNNNLDPSQIIKVVIFLAQFEGEFNVPILACIRGDQHINEVKLFNLINKL
NHFNLLNLKKIEDKKTIEKNLVDLPLGFIGPDLDNNTIKAISNWEKQWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSF
DSKDYLIGDIRNAKKGDKISVYDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIHISPFEVIIIPTNLKDPIQSDLTEQIYSNFLINKIDVLLDDRNDRAGVKFKDAELIGIPFQIIIGRD
SINKEVELLCRTNNTKLKISTDKLLETFISESEIMYNKKS

Sequences:

>Translated_600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVIEKISAIIEKELNSIGCTKLLLPQLHPADL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSQNDLASFYEKVSNAYENIFKSCGLQTVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSLPSQPIPLKDNIPEWLETPHQKTILEVCDNNNLDPSQIIKVVIFLAQFEGEFNVPILACIRGDQHINEVKLFNLINKL
NHFNLLNLKKIEDKKTIEKNLVDLPLGFIGPDLDNNTIKAISNWEKQWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSF
DSKDYLIGDIRNAKKGDKISVYDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIHISPFEVIIIPTNLKDPIQSDLTEQIYSNFLINKIDVLLDDRNDRAGVKFKDAELIGIPFQIIIGRD
SINKEVELLCRTNNTKLKISTDKLLETFISESEIMYNKKS
>Mature_600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVIEKISAIIEKELNSIGCTKLLLPQLHPADL
WKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITSIASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSQNDLASFYEKVSNAYENIFKSCGLQTVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
VSLPSQPIPLKDNIPEWLETPHQKTILEVCDNNNLDPSQIIKVVIFLAQFEGEFNVPILACIRGDQHINEVKLFNLINKL
NHFNLLNLKKIEDKKTIEKNLVDLPLGFIGPDLDNNTIKAISNWEKQWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSF
DSKDYLIGDIRNAKKGDKISVYDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
EQNHDQKGICWPIHISPFEVIIIPTNLKDPIQSDLTEQIYSNFLINKIDVLLDDRNDRAGVKFKDAELIGIPFQIIIGRD
SINKEVELLCRTNNTKLKISTDKLLETFISESEIMYNKKS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=43.6681222707424, Blast_Score=194, Evalue=2e-49,
Organism=Escherichia coli, GI1786392, Length=591, Percent_Identity=41.6243654822335, Blast_Score=451, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI115532348, Length=213, Percent_Identity=37.0892018779343, Blast_Score=135, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320931, Length=225, Percent_Identity=36.4444444444444, Blast_Score=161, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24656200, Length=226, Percent_Identity=37.6106194690265, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 68050; Mature: 68050

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVIEKISAIIEK
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELNSIGCTKLLLPQLHPADLWKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITS
HHHHCCCHHHHCCCCCHHHHHHCHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHH
IASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFIMKDGYSFHSSQNDLASFYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VSNAYENIFKSCGLQTVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHCHHHH
VSLPSQPIPLKDNIPEWLETPHQKTILEVCDNNNLDPSQIIKVVIFLAQFEGEFNVPILA
HCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
CIRGDQHINEVKLFNLINKLNHFNLLNLKKIEDKKTIEKNLVDLPLGFIGPDLDNNTIKA
EECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHH
ISNWEKQWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSFDSKDYLIGDIRNAKKGDKIS
HHHHHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHCCCCCCEEEEECCCCCCCCEEE
VYDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
ECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQNHDQKGICWPIHISPFEVIIIPTNLKDPIQSDLTEQIYSNFLINKIDVLLDDRNDRAG
HCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCC
VKFKDAELIGIPFQIIIGRDSINKEVELLCRTNNTKLKISTDKLLETFISESEIMYNKKS
CEEECCEEECCCEEEEECCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHCCCHHEECCCC
>Mature Secondary Structure
MRVTTSFPLGTLRDTPSEAEIISHQLLLKAGYIRRVNSGIYAYMPLMLRVIEKISAIIEK
CEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ELNSIGCTKLLLPQLHPADLWKKSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITS
HHHHCCCHHHHCCCCCHHHHHHCHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHH
IASETINSYKQLPQCFYQIQTKFRDEIRPRFGLMRSREFIMKDGYSFHSSQNDLASFYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VSNAYENIFKSCGLQTVGVEADSGAIGGASSKEFMVTADAGEDSILFTQSGSYAANIEKA
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHCHHHH
VSLPSQPIPLKDNIPEWLETPHQKTILEVCDNNNLDPSQIIKVVIFLAQFEGEFNVPILA
HCCCCCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEE
CIRGDQHINEVKLFNLINKLNHFNLLNLKKIEDKKTIEKNLVDLPLGFIGPDLDNNTIKA
EECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHH
ISNWEKQWTRIIDHSASDLSKFISGGNKVNFHKVFQEFSFDSKDYLIGDIRNAKKGDKIS
HHHHHHHHHHHHCCCHHHHHHHHCCCCEEHHHHHHHHHCCCCCCEEEEECCCCCCCCEEE
VYDDEELKEKKGIEIGHIFQLGQKYSEKLNAKFSDKDGQLKNLWMGCYGIGVTRIAQAAI
ECCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQNHDQKGICWPIHISPFEVIIIPTNLKDPIQSDLTEQIYSNFLINKIDVLLDDRNDRAG
HCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCC
VKFKDAELIGIPFQIIIGRDSINKEVELLCRTNNTKLKISTDKLLETFISESEIMYNKKS
CEEECCEEECCCEEEEECCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHCCCHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA