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The map label for this gene is xthA [H]

Identifier: 123968077

GI number: 123968077

Start: 469369

End: 470214

Strand: Reverse

Name: xthA [H]

Synonym: A9601_05401

Alternate gene names: 123968077

Gene position: 470214-469369 (Counterclockwise)

Preceding gene: 123968082

Following gene: 123968076

Centisome position: 28.16

GC content: 30.85

Gene sequence:

>846_bases
TTGTTAATAGCAACTTGGAATGTTAACTCTATAAGAACCAGACTTTCACAAATATTAGATTGGATTAATAAATCCAATCC
AGATATTCTATGTTTGCAGGAAACAAAAGTGGTGGATGATAATTTCCCTATTGAAGCTTTTGAAAAATTAGGATATTCAG
TAGAGATTTACGGACAGAAATCATACAATGGTGTGGCTATTATTTCCAAAATAAAGCCAGAAAATGTTAAAAAAGGATTC
TACGGTTGTAATGACACTAATCAAAATATCGAAATTTTCCTAGATCAAAAAAGATTGATTTCTGCAGATATTAATGGTAT
TAAAATCATAAATGTCTATGTTCCAAATGGATCTTCTCTAGATTCTAGTAAGTTCGAATACAAAATTAATTGGTTAAATT
GTTTAGCTTCATTTTTAGATGATCAAGAAAAAAAAGGAGCATTAATTTGTCTAATGGGTGATTTTAATGTTGCTCCATCT
AACTTGGATATTCATGATCCAAAGAAATATGAAGGAGGAATAATGGCATCTGAGATAGAGAGAAGTGCACTAAATAATGT
TCTGAAAAAAAGATTAATAGATTCGTTCAGGATTTATGAACAAAATACTGGCCATTGGAGTTGGTGGGATTACCGTAATA
ACGCATTTGAATTAAATAAAGGTTGGAGAATAGACCATATATATATCAGCAAAGAACTGTCATCAAAACTTAAAAGTTGT
GTCATAGACAGCTGCCCTAGAGGTAATTTACGCCCAAGTGATCATGCCCCAGTAATGATAGATCTTAACTTAAACGAAAT
AAATGCAGATTTTTTTGAGGATGAGGATAATTTCTTCGAAATATAA

Upstream 100 bases:

>100_bases
AAACGCTTTTGCAAAAATAATTTTTCATTTTATTTGATTTCAAAATTCTTTAAAGCCAAAAATTTTTTGCTATTTTAATT
TTACATAATTTGGGAGAAAT

Downstream 100 bases:

>100_bases
ATGAAATAAATTGAATTTCTTTAATGTCTGAAAACGCTTATTAATAAAGAGTTATCGTAAGTAAGATTGTTTTTTATCAT
GATTTCTTTAAAATTAATAA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MLIATWNVNSIRTRLSQILDWINKSNPDILCLQETKVVDDNFPIEAFEKLGYSVEIYGQKSYNGVAIISKIKPENVKKGF
YGCNDTNQNIEIFLDQKRLISADINGIKIINVYVPNGSSLDSSKFEYKINWLNCLASFLDDQEKKGALICLMGDFNVAPS
NLDIHDPKKYEGGIMASEIERSALNNVLKKRLIDSFRIYEQNTGHWSWWDYRNNAFELNKGWRIDHIYISKELSSKLKSC
VIDSCPRGNLRPSDHAPVMIDLNLNEINADFFEDEDNFFEI

Sequences:

>Translated_281_residues
MLIATWNVNSIRTRLSQILDWINKSNPDILCLQETKVVDDNFPIEAFEKLGYSVEIYGQKSYNGVAIISKIKPENVKKGF
YGCNDTNQNIEIFLDQKRLISADINGIKIINVYVPNGSSLDSSKFEYKINWLNCLASFLDDQEKKGALICLMGDFNVAPS
NLDIHDPKKYEGGIMASEIERSALNNVLKKRLIDSFRIYEQNTGHWSWWDYRNNAFELNKGWRIDHIYISKELSSKLKSC
VIDSCPRGNLRPSDHAPVMIDLNLNEINADFFEDEDNFFEI
>Mature_281_residues
MLIATWNVNSIRTRLSQILDWINKSNPDILCLQETKVVDDNFPIEAFEKLGYSVEIYGQKSYNGVAIISKIKPENVKKGF
YGCNDTNQNIEIFLDQKRLISADINGIKIINVYVPNGSSLDSSKFEYKINWLNCLASFLDDQEKKGALICLMGDFNVAPS
NLDIHDPKKYEGGIMASEIERSALNNVLKKRLIDSFRIYEQNTGHWSWWDYRNNAFELNKGWRIDHIYISKELSSKLKSC
VIDSCPRGNLRPSDHAPVMIDLNLNEINADFFEDEDNFFEI

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=272, Percent_Identity=32.7205882352941, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI18375503, Length=272, Percent_Identity=32.7205882352941, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI18375501, Length=272, Percent_Identity=32.7205882352941, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=35.1851851851852, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=27.4436090225564, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI221330655, Length=268, Percent_Identity=32.4626865671642, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI17136678, Length=268, Percent_Identity=32.4626865671642, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32336; Mature: 32336

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIATWNVNSIRTRLSQILDWINKSNPDILCLQETKVVDDNFPIEAFEKLGYSVEIYGQK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCHHHHHHCCCEEEEEECC
SYNGVAIISKIKPENVKKGFYGCNDTNQNIEIFLDQKRLISADINGIKIINVYVPNGSSL
CCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCEEEEEEEECCCCCC
DSSKFEYKINWLNCLASFLDDQEKKGALICLMGDFNVAPSNLDIHDPKKYEGGIMASEIE
CCCCEEEEEHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHH
RSALNNVLKKRLIDSFRIYEQNTGHWSWWDYRNNAFELNKGWRIDHIYISKELSSKLKSC
HHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCEEEECCCEEEEEEEECHHHHHHHHHH
VIDSCPRGNLRPSDHAPVMIDLNLNEINADFFEDEDNFFEI
HHCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCCEECC
>Mature Secondary Structure
MLIATWNVNSIRTRLSQILDWINKSNPDILCLQETKVVDDNFPIEAFEKLGYSVEIYGQK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEECCEEECCCCCHHHHHHCCCEEEEEECC
SYNGVAIISKIKPENVKKGFYGCNDTNQNIEIFLDQKRLISADINGIKIINVYVPNGSSL
CCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCEEEECCCCCEEEEEEEECCCCCC
DSSKFEYKINWLNCLASFLDDQEKKGALICLMGDFNVAPSNLDIHDPKKYEGGIMASEIE
CCCCEEEEEHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEHHHHH
RSALNNVLKKRLIDSFRIYEQNTGHWSWWDYRNNAFELNKGWRIDHIYISKELSSKLKSC
HHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCEEEECCCEEEEEEEECHHHHHHHHHH
VIDSCPRGNLRPSDHAPVMIDLNLNEINADFFEDEDNFFEI
HHCCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]