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The map label for this gene is nth [H]

Identifier: 123966014

GI number: 123966014

Start: 704682

End: 705335

Strand: Direct

Name: nth [H]

Synonym: P9515_07791

Alternate gene names: 123966014

Gene position: 704682-705335 (Clockwise)

Preceding gene: 123966011

Following gene: 123966015

Centisome position: 41.35

GC content: 30.58

Gene sequence:

>654_bases
ATGAAAAAGAGTGAAAGAGCAGAAATAATTCTTAAAGAACTAAATAAACTATATCCATCACCACCAATTCCTCTTGATCA
TACTAATGCATTTACTCTTCTTGTTGCAGTGGTATTAAGTGCCCAGTCAACAGATAAGAAAGTTAATGAATTAACTAAGA
AATTATTCAAAGTCGCAGATACTCCGCAAAAAATGGTAGAACTTGGTGTTTCTAAAATTTATGAGTACATAAAACAATTA
GGTCTATCAAACCAAAAATCTAAAAATATTTACCTTTTATCAAAATTAATAGTCAATAAATTTAATTATCAAGTACCTAA
TTCCTTCGAAGATCTTGAATCACTTCCAGGAGTTGGCCATAAAACTGCTTCAGTTATTATGTCTCAAGTCTTTAATATCC
CCTCTTTCCCTGTTGATACACATATACATCGTTTATCTCAAAGATGGGGTCTTACAAAAGGAGATAATGTAAGGCAAACA
GAGAAAGACCTTAAAAATTTATTTCCAATCTCAGAGTGGAATAAATTGCATTTACAAATTATCTTTTACGGAAGAGAATT
TTGTACGGCAAGAGGATGTGATGGAACAAAATGCTTAATGTGCAGAACATTATATCCTAATAGAAAAAAGAAATTTGTAT
GTAAAAAGCCTTAA

Upstream 100 bases:

>100_bases
TTGTCACAAAAAAATTTTTTATCTAATTTTTTAAATTAGCAGAAAATATTCTTTCCCTATAGCACCCGTATAATTTTATA
CAGCCACTAAAATAAAATTA

Downstream 100 bases:

>100_bases
AATTTTATTATACTAACCATGTTTGTTTTAAAATTATGAGAATAGCAATTACTGGAGCTTCCGGTAAAACAGGTTACAGA
ATAGTCGAAGAAGCTCAAAA

Product: putative endonuclease

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MKKSERAEIILKELNKLYPSPPIPLDHTNAFTLLVAVVLSAQSTDKKVNELTKKLFKVADTPQKMVELGVSKIYEYIKQL
GLSNQKSKNIYLLSKLIVNKFNYQVPNSFEDLESLPGVGHKTASVIMSQVFNIPSFPVDTHIHRLSQRWGLTKGDNVRQT
EKDLKNLFPISEWNKLHLQIIFYGREFCTARGCDGTKCLMCRTLYPNRKKKFVCKKP

Sequences:

>Translated_217_residues
MKKSERAEIILKELNKLYPSPPIPLDHTNAFTLLVAVVLSAQSTDKKVNELTKKLFKVADTPQKMVELGVSKIYEYIKQL
GLSNQKSKNIYLLSKLIVNKFNYQVPNSFEDLESLPGVGHKTASVIMSQVFNIPSFPVDTHIHRLSQRWGLTKGDNVRQT
EKDLKNLFPISEWNKLHLQIIFYGREFCTARGCDGTKCLMCRTLYPNRKKKFVCKKP
>Mature_217_residues
MKKSERAEIILKELNKLYPSPPIPLDHTNAFTLLVAVVLSAQSTDKKVNELTKKLFKVADTPQKMVELGVSKIYEYIKQL
GLSNQKSKNIYLLSKLIVNKFNYQVPNSFEDLESLPGVGHKTASVIMSQVFNIPSFPVDTHIHRLSQRWGLTKGDNVRQT
EKDLKNLFPISEWNKLHLQIIFYGREFCTARGCDGTKCLMCRTLYPNRKKKFVCKKP

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Escherichia coli, GI1787920, Length=200, Percent_Identity=41, Blast_Score=152, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17554540, Length=195, Percent_Identity=29.2307692307692, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=184, Percent_Identity=25.5434782608696, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=23.5632183908046, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550361, Length=190, Percent_Identity=28.9473684210526, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24902; Mature: 24902

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSERAEIILKELNKLYPSPPIPLDHTNAFTLLVAVVLSAQSTDKKVNELTKKLFKVAD
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
TPQKMVELGVSKIYEYIKQLGLSNQKSKNIYLLSKLIVNKFNYQVPNSFEDLESLPGVGH
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCH
KTASVIMSQVFNIPSFPVDTHIHRLSQRWGLTKGDNVRQTEKDLKNLFPISEWNKLHLQI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEE
IFYGREFCTARGCDGTKCLMCRTLYPNRKKKFVCKKP
EEECHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKSERAEIILKELNKLYPSPPIPLDHTNAFTLLVAVVLSAQSTDKKVNELTKKLFKVAD
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
TPQKMVELGVSKIYEYIKQLGLSNQKSKNIYLLSKLIVNKFNYQVPNSFEDLESLPGVGH
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCH
KTASVIMSQVFNIPSFPVDTHIHRLSQRWGLTKGDNVRQTEKDLKNLFPISEWNKLHLQI
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEE
IFYGREFCTARGCDGTKCLMCRTLYPNRKKKFVCKKP
EEECHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]