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The map label for this gene is 123965548

Identifier: 123965548

GI number: 123965548

Start: 288944

End: 290431

Strand: Reverse

Name: 123965548

Synonym: P9515_03131

Alternate gene names: NA

Gene position: 290431-288944 (Counterclockwise)

Preceding gene: 123965549

Following gene: 123965545

Centisome position: 17.04

GC content: 34.14

Gene sequence:

>1488_bases
GTGACTTATATTCAAGACAAACAAATTAAGACTAATCAAGCTTTTAATAAAGAAGATTGGTCTAGCGCATATAAAAATGT
TGAAACAGAATTAACAAATGAATCATTAAAGATTACTAAAGGCGAAAATATTGTTGAATTAAACGGAACTTTGCTTAGAA
ACGGGCCCGGCATATTAGAGAGAGGAGGTCAATGGGTTCATCATCCATTCGATGGAGATGGCATGATCACTGCAATTAAC
TTTAAGAATGGGAAACCATCATTAACAAATAAGTTTGTGAGAACAAAAGGTTATATAGAGGAAACAAAGATTAATAAATT
TGTTTATAGAGGCGTTTTTGGTACTCAAAAAAGTGGGGGCATATTAAATAATGCTCTGGATTTAAAATTCAAAAATATTG
CTAATACACACGTTGTAAAACTAGGTAATGAAGTTCTCGCACTATGGGAAGCAGCAGGTCCACATGCCTTAGAACCAAGT
AATCTAGATACAAAAGGATTAACAACTTTGAATGGGGTTTTAAAAGCCAATGAAGCTTTTAGTGCTCATCCAAAAGCAGA
TTTCAATTCAAATTATTCCTCTGAAGTCATGGTGACTTTTGGAGTACAAACCGGGCCAAAAAGCACTATTAGATTAATGG
AATTTTCAAATGCTGGTGAAAATTCTGGTGAATTGATATTTGACAGGAAAGATACATTTAATGGTTTTGCATTCCTTCAT
GATTTCGCAATTACAACCAATTGGGCAATATTTTTACAGAATGCTATCGACTTCAACCCACTGCCATTTGTGATGGGCCA
AAAAGGAGCCGCTCAATGTTTGAAATCAAATCCCAATAAAAAAGCAAAATTTTTTATAATCCCAAGGGAAAGCGGTTTAT
TTAAGGGGCAACCATCAATAACTATAGATGCCCCAGAGGGATTTGTTTTTCATCATGTTAATGCATTCGAAAAGGATTCA
AACATAATTTTAGATAGTATTTTTTATGATGATTTCCCTTCTGTTGGTCCAGACGAAAATTTTAGGGATATTGATTTTGA
TCAATATCCAGCTGGGAATTTAAAAAGATCTATTATCGACCTTAAAGGGAAAAAGTCTCAATTTGAAACTTTAAGTACAC
AAACTTGCGAATTCGCTACTGTTAATCCCAAATTCTTAGGATTAAAAGCGACTCATTCATGGATGGCTTGTACTGATAAG
AAGACAGGAAATGCTCCTCTACAGGCTATAAAGAAAATTAATTTGACTACCAAAGAGGAAATTTATTGGTCAGCAGCACC
TAGTGGATTCGTTAGTGAACCAATAATGGTCCCATCACAAATATCAAATAAAGAAGATGACGGTTACTTATTTGTTCTGA
TATGGAATGGTAGTAGAAGAGGAAGTGATCTTGTGATATTAGATGCCAATGATTTAAAAGAATTAGCTATTTATGAGCTT
CCGATATCAATTCCACATGGCCTGCATGGATCTTGGGTTAATAATTAA

Upstream 100 bases:

>100_bases
GAATGCTAGAAATTGATTAGCTATACAATTTAAGTATTTTTTTTAATAAGCCACAATTTTATAGATTTTTGGCGAAAATA
AATAAAAATAAAACTTTAAA

Downstream 100 bases:

>100_bases
TTATGCAAATATTTCTAACAATTGAGGAATAATTTTTTTTAATGCTTTGCCACGATGACTACATTCATCTTTTAATTCAT
TATTCATCTCAGCGAAAGTT

Product: retinal pigment epithelial membrane protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTYIQDKQIKTNQAFNKEDWSSAYKNVETELTNESLKITKGENIVELNGTLLRNGPGILERGGQWVHHPFDGDGMITAIN
FKNGKPSLTNKFVRTKGYIEETKINKFVYRGVFGTQKSGGILNNALDLKFKNIANTHVVKLGNEVLALWEAAGPHALEPS
NLDTKGLTTLNGVLKANEAFSAHPKADFNSNYSSEVMVTFGVQTGPKSTIRLMEFSNAGENSGELIFDRKDTFNGFAFLH
DFAITTNWAIFLQNAIDFNPLPFVMGQKGAAQCLKSNPNKKAKFFIIPRESGLFKGQPSITIDAPEGFVFHHVNAFEKDS
NIILDSIFYDDFPSVGPDENFRDIDFDQYPAGNLKRSIIDLKGKKSQFETLSTQTCEFATVNPKFLGLKATHSWMACTDK
KTGNAPLQAIKKINLTTKEEIYWSAAPSGFVSEPIMVPSQISNKEDDGYLFVLIWNGSRRGSDLVILDANDLKELAIYEL
PISIPHGLHGSWVNN

Sequences:

>Translated_495_residues
MTYIQDKQIKTNQAFNKEDWSSAYKNVETELTNESLKITKGENIVELNGTLLRNGPGILERGGQWVHHPFDGDGMITAIN
FKNGKPSLTNKFVRTKGYIEETKINKFVYRGVFGTQKSGGILNNALDLKFKNIANTHVVKLGNEVLALWEAAGPHALEPS
NLDTKGLTTLNGVLKANEAFSAHPKADFNSNYSSEVMVTFGVQTGPKSTIRLMEFSNAGENSGELIFDRKDTFNGFAFLH
DFAITTNWAIFLQNAIDFNPLPFVMGQKGAAQCLKSNPNKKAKFFIIPRESGLFKGQPSITIDAPEGFVFHHVNAFEKDS
NIILDSIFYDDFPSVGPDENFRDIDFDQYPAGNLKRSIIDLKGKKSQFETLSTQTCEFATVNPKFLGLKATHSWMACTDK
KTGNAPLQAIKKINLTTKEEIYWSAAPSGFVSEPIMVPSQISNKEDDGYLFVLIWNGSRRGSDLVILDANDLKELAIYEL
PISIPHGLHGSWVNN
>Mature_494_residues
TYIQDKQIKTNQAFNKEDWSSAYKNVETELTNESLKITKGENIVELNGTLLRNGPGILERGGQWVHHPFDGDGMITAINF
KNGKPSLTNKFVRTKGYIEETKINKFVYRGVFGTQKSGGILNNALDLKFKNIANTHVVKLGNEVLALWEAAGPHALEPSN
LDTKGLTTLNGVLKANEAFSAHPKADFNSNYSSEVMVTFGVQTGPKSTIRLMEFSNAGENSGELIFDRKDTFNGFAFLHD
FAITTNWAIFLQNAIDFNPLPFVMGQKGAAQCLKSNPNKKAKFFIIPRESGLFKGQPSITIDAPEGFVFHHVNAFEKDSN
IILDSIFYDDFPSVGPDENFRDIDFDQYPAGNLKRSIIDLKGKKSQFETLSTQTCEFATVNPKFLGLKATHSWMACTDKK
TGNAPLQAIKKINLTTKEEIYWSAAPSGFVSEPIMVPSQISNKEDDGYLFVLIWNGSRRGSDLVILDANDLKELAIYELP
ISIPHGLHGSWVNN

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI4506591, Length=526, Percent_Identity=23.384030418251, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI32564756, Length=549, Percent_Identity=23.4972677595628, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 55024; Mature: 54893

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYIQDKQIKTNQAFNKEDWSSAYKNVETELTNESLKITKGENIVELNGTLLRNGPGILE
CCCCCCCCCCCCCCCCHHHHHHHHHCCHHEECCCEEEEECCCCEEEECCEEEECCCCHHH
RGGQWVHHPFDGDGMITAINFKNGKPSLTNKFVRTKGYIEETKINKFVYRGVFGTQKSGG
CCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCCCC
ILNNALDLKFKNIANTHVVKLGNEVLALWEAAGPHALEPSNLDTKGLTTLNGVLKANEAF
EECCEEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHEECCCCC
SAHPKADFNSNYSSEVMVTFGVQTGPKSTIRLMEFSNAGENSGELIFDRKDTFNGFAFLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEE
DFAITTNWAIFLQNAIDFNPLPFVMGQKGAAQCLKSNPNKKAKFFIIPRESGLFKGQPSI
EEHHHCCEEEEEECCCCCCCCCCEECCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCE
TIDAPEGFVFHHVNAFEKDSNIILDSIFYDDFPSVGPDENFRDIDFDQYPAGNLKRSIID
EEECCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEE
LKGKKSQFETLSTQTCEFATVNPKFLGLKATHSWMACTDKKTGNAPLQAIKKINLTTKEE
CCCCCCHHHHHCCCCEEEEECCCEEEEEEECCCEEEECCCCCCCCHHHHHHHCCCCCHHE
IYWSAAPSGFVSEPIMVPSQISNKEDDGYLFVLIWNGSRRGSDLVILDANDLKELAIYEL
EEEECCCCCCCCCCEECCHHCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCEEEEEEE
PISIPHGLHGSWVNN
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TYIQDKQIKTNQAFNKEDWSSAYKNVETELTNESLKITKGENIVELNGTLLRNGPGILE
CCCCCCCCCCCCCCCHHHHHHHHHCCHHEECCCEEEEECCCCEEEECCEEEECCCCHHH
RGGQWVHHPFDGDGMITAINFKNGKPSLTNKFVRTKGYIEETKINKFVYRGVFGTQKSGG
CCCCEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHEEEECCCCCCCCCC
ILNNALDLKFKNIANTHVVKLGNEVLALWEAAGPHALEPSNLDTKGLTTLNGVLKANEAF
EECCEEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHEECCCCC
SAHPKADFNSNYSSEVMVTFGVQTGPKSTIRLMEFSNAGENSGELIFDRKDTFNGFAFLH
CCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEE
DFAITTNWAIFLQNAIDFNPLPFVMGQKGAAQCLKSNPNKKAKFFIIPRESGLFKGQPSI
EEHHHCCEEEEEECCCCCCCCCCEECCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCE
TIDAPEGFVFHHVNAFEKDSNIILDSIFYDDFPSVGPDENFRDIDFDQYPAGNLKRSIID
EEECCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEE
LKGKKSQFETLSTQTCEFATVNPKFLGLKATHSWMACTDKKTGNAPLQAIKKINLTTKEE
CCCCCCHHHHHCCCCEEEEECCCEEEEEEECCCEEEECCCCCCCCHHHHHHHCCCCCHHE
IYWSAAPSGFVSEPIMVPSQISNKEDDGYLFVLIWNGSRRGSDLVILDANDLKELAIYEL
EEEECCCCCCCCCCEECCHHCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCEEEEEEE
PISIPHGLHGSWVNN
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]