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The map label for this gene is cobS [H]

Identifier: 123965538

GI number: 123965538

Start: 279303

End: 280004

Strand: Reverse

Name: cobS [H]

Synonym: P9515_03031

Alternate gene names: 123965538

Gene position: 280004-279303 (Counterclockwise)

Preceding gene: 123965541

Following gene: 123965536

Centisome position: 16.43

GC content: 27.78

Gene sequence:

>702_bases
TTGCCCTTTATTACTCCAAAATTTAAAAATATTGCTCAATTTTCCCCAATAATAGGATTTTTCATAGGGTTAATACAAAG
TCTTATTTATCTGATTTTAAGAAACAATTCTTGGTCTGCTTATGCATCCGTTCCAATTTGTGTGGCTTCTGGGTATTTAA
TTACTGGGGGTCTTCATCTAGATGGCTTAATGGATACTTTTGATGGTATTTTTGCAGGCAAAAATAAATGTTTAAAAGCT
ATGAAAGATAGTCAAGTTGGTGCATTTGGAGTTCAATCATTATTTTTTATAACTTTAATGCAAATTGCATGTCTACTAAA
AATTCAAAGCCAAATAATTTCAATATTACCTATTTGCCTATTTTGGGGAAGGTTTTCAACATTAATCTTTATAGATAAAT
TTCAATATATAACTCTTGAGACAAAGTCAATACAGCACAAAAAATACTGGAATGGTTTTAAAAAAGAATCTTTAGTTTCT
TTAACTTTTATTTTTATTTTTATTTTTTATTTTTTATTTTTAGATAAATCAGCTGACTTTATAATTAAACAAATTTTTCT
ATTATTGATAGGATTTTTTATAAGTTATGCAATTCCCAAATTTTTAGGTAGTAAAATTGGTGGATTCAATGGAGATGCCT
GCGGAGCAAGCGTGGTGCTTGTTGAAACAACAATGTTGTTTATAAATGCAGTTCTTTTATAG

Upstream 100 bases:

>100_bases
CTACTCTATTATTCAATAACTAAGTATCAGTAGTCTCATCAAAAACAATTTCATTAATAATCTTGCAGGAGCTTGGATTT
TTTATACTACTTTTCCAAAG

Downstream 100 bases:

>100_bases
GAAGTTCTAAATAAAATATGTTTTTCTTTTTAAGAGCATTTAATTCTTCATTATTATCTTTCATAGAATTATCTACTATG
TTTAAATCTCCTCCAATTTG

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MPFITPKFKNIAQFSPIIGFFIGLIQSLIYLILRNNSWSAYASVPICVASGYLITGGLHLDGLMDTFDGIFAGKNKCLKA
MKDSQVGAFGVQSLFFITLMQIACLLKIQSQIISILPICLFWGRFSTLIFIDKFQYITLETKSIQHKKYWNGFKKESLVS
LTFIFIFIFYFLFLDKSADFIIKQIFLLLIGFFISYAIPKFLGSKIGGFNGDACGASVVLVETTMLFINAVLL

Sequences:

>Translated_233_residues
MPFITPKFKNIAQFSPIIGFFIGLIQSLIYLILRNNSWSAYASVPICVASGYLITGGLHLDGLMDTFDGIFAGKNKCLKA
MKDSQVGAFGVQSLFFITLMQIACLLKIQSQIISILPICLFWGRFSTLIFIDKFQYITLETKSIQHKKYWNGFKKESLVS
LTFIFIFIFYFLFLDKSADFIIKQIFLLLIGFFISYAIPKFLGSKIGGFNGDACGASVVLVETTMLFINAVLL
>Mature_232_residues
PFITPKFKNIAQFSPIIGFFIGLIQSLIYLILRNNSWSAYASVPICVASGYLITGGLHLDGLMDTFDGIFAGKNKCLKAM
KDSQVGAFGVQSLFFITLMQIACLLKIQSQIISILPICLFWGRFSTLIFIDKFQYITLETKSIQHKKYWNGFKKESLVSL
TFIFIFIFYFLFLDKSADFIIKQIFLLLIGFFISYAIPKFLGSKIGGFNGDACGASVVLVETTMLFINAVLL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=219, Percent_Identity=27.8538812785388, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26186; Mature: 26055

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFITPKFKNIAQFSPIIGFFIGLIQSLIYLILRNNSWSAYASVPICVASGYLITGGLHL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHCCEEEECCCCH
DGLMDTFDGIFAGKNKCLKAMKDSQVGAFGVQSLFFITLMQIACLLKIQSQIISILPICL
HHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWGRFSTLIFIDKFQYITLETKSIQHKKYWNGFKKESLVSLTFIFIFIFYFLFLDKSADF
HHHHHHHHHEEECEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
IIKQIFLLLIGFFISYAIPKFLGSKIGGFNGDACGASVVLVETTMLFINAVLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFITPKFKNIAQFSPIIGFFIGLIQSLIYLILRNNSWSAYASVPICVASGYLITGGLHL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHCCEEEECCCCH
DGLMDTFDGIFAGKNKCLKAMKDSQVGAFGVQSLFFITLMQIACLLKIQSQIISILPICL
HHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FWGRFSTLIFIDKFQYITLETKSIQHKKYWNGFKKESLVSLTFIFIFIFYFLFLDKSADF
HHHHHHHHHEEECEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHH
IIKQIFLLLIGFFISYAIPKFLGSKIGGFNGDACGASVVLVETTMLFINAVLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]