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The map label for this gene is leuA [H]

Identifier: 121639626

GI number: 121639626

Start: 4126856

End: 4129360

Strand: Direct

Name: leuA [H]

Synonym: BCG_3770

Alternate gene names: 121639626

Gene position: 4126856-4129360 (Clockwise)

Preceding gene: 121639614

Following gene: 121639628

Centisome position: 94.34

GC content: 68.58

Gene sequence:

>2505_bases
GTGACAACTTCTGAATCGCCCGACGCCTATACCGAGTCGTTTGGGGCCCACACCATCGTGAAACCCGCCGGCCCACCTCG
CGTCGGTCAGCCCTCGTGGAATCCGCAGCGAGCCTCGTCGATGCCGGTCAACCGCTACCGGCCGTTCGCCGAGGAGGTCG
AGCCCATCCGGCTGAGAAACCGCACGTGGCCTGATCGCGTCATCGATCGTGCGCCGCTGTGGTGCGCGGTCGACTTACGC
GATGGCAACCAGGCGCTGATCGACCCGATGAGCCCGGCCCGCAAGCGCCGCATGTTCGACCTGCTGGTCCGGATGGGCTA
CAAGGAGATTGAGGTGGGGTTCCCCTCGGCCAGCCAGACCGACTTCGACTTCGTCAGAGAGATCATCGAGCAGGGCGCCA
TTCCCGACGACGTCACCATCCAGGTGCTCACCCAATGCCGTCCCGAGCTGATCGAGCGCACCTTCCAGGCGTGTTCGGGC
GCACACCGGGCCATCGTGCACTTCTACAACTCGACGTCAATCCTGCAGCGCCGCGTGGTCTTTCGCGCCAACCGGGCTGA
GGTGCAGGCCATCGCGACAGATGGGGCGCGCAAGTGCGTCGAGCAGGCCGCCAAATACCCGGGCACGCAGTGGCGATTCG
AGTACTCCCCGGAGTCCTACACCGGCACCGAACTGGAATACGCCAAACAGGTGTGCGACGCCGTCGGCGAGGTCATTGCG
CCGACGCCGGAGCGCCCGATCATCTTCAACCTGCCCGCCACGGTGGAGATGACGACGCCCAATGTCTACGCCGACTCGAT
CGAGTGGATGAGCCGCAACCTAGCCAACCGGGAGTCGGTCATCCTGAGCCTGCACCCGCACAATGACCGCGGAACCGCCG
TCGCCGCAGCGGAATTGGGTTTCGCGGCCGGGGCTGATCGGATCGAGGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGC
AACGTGTGCCTGGTCACGCTGGGACTCAACCTGTTCTCCCGAGGTGTGGACCCGCAGATCGACTTCTCCAATATTGACGA
GATCCGGCGCACGGTGGAGTACTGCAACCAGCTGCCGGTGCACGAACGTCACCCCTATGGCGGCGACCTGGTGTACACCG
CGTTCTCCGGTAGCCACCAGGACGCCATCAACAAGGGCCTAGACGCGATGAAGCTGGATGCGGATGCCGCCGACTGTGAC
GTCGACGACATGCTGTGGCAGGTGCCGTATCTGCCCATCGACCCGCGCGATGTCGGGCGCACCTACGAGGCGGTGATCCG
GGTCAACTCGCAGTCCGGCAAGGGCGGCGTGGCCTACATCATGAAGACCGACCACGGCCTTTCCCTGCCGCGGCGGCTGC
AGATCGAGTTTTCCCAGGTAATCCAGAAGATCGCAGAGGGTACAGCAGGCGAGGGTGGCGAGGTCTCGCCCAAGGAGATG
TGGGATGCGTTCGCCGAGGAGTATCTGGCCCCGGTGCGGCCTTTGGAGCGGATAAGGCAACATGTGGACGCTGCCGACGA
CGACGGCGGCACGACCAGCATCACGGCGACCGTCAAGATCAACGGCGTGGAGACCGAGATCAGCGGGTCCGGTAACGGTC
CGTTGGCCGCGTTCGTCCATGCGCTGGCCGATGTCGGGTTTGACGTGGCCGTGCTGGACTACTACGAGCACGCGATGAGC
GCCGGCGACGACGCTCAGGCCGCCGCGTATGTGGAGGCCTCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGG
TCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGA
CGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCG
GGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATC
GGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCC
CGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGT
CGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGAC
GATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGG
GCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCGCAGCCGGGCGAAGCGGGTCGGCACGCATCG
GACCCCGTGACGAGTAAGACGGTGTGGGGTGTCGGTATCGCACCGTCAATCACCACCGCGTCGCTGCGCGCCGTGGTGTC
GGCGGTCAACCGGGCGGCACGCTAG

Upstream 100 bases:

>100_bases
CGGACGCCGCGACGGGGTCTGATCCAGACCGGCTTCCCGTCGCGGGACGTTCGCGATGCGCCGGTCTGAGGTTCCTTCTC
ACCATCCCGGAGCAACTACC

Downstream 100 bases:

>100_bases
GACGGCGCTGAACTAGGGTCGGGGTCCGCGGCATGATTTTTCGCAGTGACGTTCCGCTCGCCGTTTCAGAACAACGCTAA
CTGCTTTTCGACGGGAGCGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 834; Mature: 833

Protein sequence:

>834_residues
MTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLR
DGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSG
AHRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA
PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTG
NVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCD
VDDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM
WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVHALADVGFDVAVLDYYEHAMS
AGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQP
GEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAG
RHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHAS
DPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR

Sequences:

>Translated_834_residues
MTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLR
DGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSG
AHRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA
PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTG
NVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCD
VDDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM
WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVHALADVGFDVAVLDYYEHAMS
AGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQP
GEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAG
RHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHAS
DPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR
>Mature_833_residues
TTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLRD
GNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGA
HRAIVHFYNSTSILQRRVVFRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIAP
TPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELGFAAGADRIEGCLFGNGERTGN
VCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPVHERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDV
DDMLWQVPYLPIDPRDVGRTYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEMW
DAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVHALADVGFDVAVLDYYEHAMSA
GDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPG
EAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGR
HASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASD
PVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI238624188, Length=222, Percent_Identity=31.981981981982, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1786261, Length=312, Percent_Identity=28.8461538461538, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324225, Length=539, Percent_Identity=47.6808905380334, Blast_Score=500, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6324682, Length=538, Percent_Identity=46.4684014869888, Blast_Score=487, Evalue=1e-138,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 88584; Mature: 88453

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRN
CCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHCCHHHHCC
RTWPDRVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQT
CCCHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
DFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGAHRAIVHFYNSTSILQRRVV
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
FRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA
HHCCHHHEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELG
CCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHH
FAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPV
HHCCHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
HERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDVDDMLWQVPYLPIDPRDVGR
CCCCCCCCCEEEEEECCCCHHHHHCCCCCEEECCCCCCCCHHHHHHHCCCCCCCHHHHCC
TYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM
CEEEEEEECCCCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHH
WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVH
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECEEEEECCCCCCHHHHHHH
ALADVGFDVAVLDYYEHAMSAGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPA
HHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCC
QPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPG
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCC
EAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAG
CCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCC
RHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHA
CCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
SDPVTIASPAQPGEAGRHASDPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR
CCCEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRN
CCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCHHHCCHHHHCC
RTWPDRVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQT
CCCHHHHHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCC
DFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGAHRAIVHFYNSTSILQRRVV
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
FRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA
HHCCHHHEEEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHC
PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELG
CCCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHH
FAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPV
HHCCHHHHCEEEECCCCCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCC
HERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDVDDMLWQVPYLPIDPRDVGR
CCCCCCCCCEEEEEECCCCHHHHHCCCCCEEECCCCCCCCHHHHHHHCCCCCCCHHHHCC
TYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM
CEEEEEEECCCCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCHHHH
WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVH
HHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECEEEEECCCCCCHHHHHHH
ALADVGFDVAVLDYYEHAMSAGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPA
HHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCC
QPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPG
CCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCC
EAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAG
CCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCC
RHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHASDPVTIASPAQPGEAGRHA
CCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
SDPVTIASPAQPGEAGRHASDPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR
CCCEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]