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The map label for this gene is ephE [H]

Identifier: 121639583

GI number: 121639583

Start: 4084459

End: 4085442

Strand: Direct

Name: ephE [H]

Synonym: BCG_3728

Alternate gene names: 121639583

Gene position: 4084459-4085442 (Clockwise)

Preceding gene: 121639582

Following gene: 121639588

Centisome position: 93.37

GC content: 67.99

Gene sequence:

>984_bases
ATGGCGGCACCAGATCCGTCGATGACCCGCATCGCCGGGCCATGGCGTCATCTGGACGTGCACGCCAACGGCATCCGATT
CCACGTCGTCGAGGCTGTGCCGTCCGGCCAGCCGGAGGGCCCGGATGCGGCTACGCCCCCCATGCAGCCGGCCCTGGCGA
GGCCGCTGGTCATACTGCTCCATGGTTTCGGCTCGTTCTGGTGGTCCTGGCGTCATCAGTTGTGCGGCCTGACCGGGGCG
CGGGTGGTCGCGGTCGATCTGCGCGGCTACGGCGGCAGCGACAAACCGCCCCGCGGGTACGACGGCTGGACGCTGGCCGG
CGATACGGCCGGTCTCATCCGTGCGCTCGGGCACCCATCGGCGACGCTGGTCGGCCACGCCGATGGCGGACTGGCCTGCT
GGACCACCGCGCTGCTGCATTCGCGGCTGGTGCGCGCCATAGCGCTGATCAGCTCACCGCACCCCGCCGCGCTACGGCGA
TCCACGCTGACCCGGCGTGATCAGCGGCACGCACTGTTACCGACATTGCTGCGTTACCAGCTGCCGATCTGGCCGGAGCG
CTTGCTGACCCGCAACAACGCAGCGGAGATCGAGCGCCTCGTGCGCGCCCGTGGCTGCGCCAAATGGCTTGCATCCGAGG
ACTTCTCGCAAGCAATCGACCACCTTCGACAGGCGATCCAGATCCCGGCGGCGGCGCATTGCGCACTCGAGTACCAGCGC
TGGGCGGTGCGCAGCCAGCTGCGCAGCGAAGGGCGGCGATTCATCAGGGCGATGACACAGCAACTGGGGATGCCGCTGCT
GCACTTACGAGGCGACGCCGACCCTTACGTGCTGGCCGACCCGGTAGAGCGCACCCAGCGCTACGCACCACACGGGCGGT
ACATATCCATTGCCGGCGCAGGACATTTCAGTCACGAAGAGGCGCCGGAGGAAGTCAACCGACATCTGATGCGTTTCCTC
GAGCAGGTGCACCAGCTCAGCTGA

Upstream 100 bases:

>100_bases
GACGCGCACCGCACTTACCCCGGGCCATGACAAAACCCCTGTGACACCAAAACCCGTCACATCTGATCGCGCGACGCCGG
TTGACCCCTCGGGTTGGTAG

Downstream 100 bases:

>100_bases
CGCAGGCCCCGGTGCCGACCGGTTGGGTAGCACCGATTTTGGCAAGCTGCCCCGCCACCTCGCCGGCCGTCAGCACAAAC
CCAGTTTCGGCGTCGTCGAC

Product: putative epoxide hydrolase ephE

Products: glycol

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAAPDPSMTRIAGPWRHLDVHANGIRFHVVEAVPSGQPEGPDAATPPMQPALARPLVILLHGFGSFWWSWRHQLCGLTGA
RVVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHPSATLVGHADGGLACWTTALLHSRLVRAIALISSPHPAALRR
STLTRRDQRHALLPTLLRYQLPIWPERLLTRNNAAEIERLVRARGCAKWLASEDFSQAIDHLRQAIQIPAAAHCALEYQR
WAVRSQLRSEGRRFIRAMTQQLGMPLLHLRGDADPYVLADPVERTQRYAPHGRYISIAGAGHFSHEEAPEEVNRHLMRFL
EQVHQLS

Sequences:

>Translated_327_residues
MAAPDPSMTRIAGPWRHLDVHANGIRFHVVEAVPSGQPEGPDAATPPMQPALARPLVILLHGFGSFWWSWRHQLCGLTGA
RVVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHPSATLVGHADGGLACWTTALLHSRLVRAIALISSPHPAALRR
STLTRRDQRHALLPTLLRYQLPIWPERLLTRNNAAEIERLVRARGCAKWLASEDFSQAIDHLRQAIQIPAAAHCALEYQR
WAVRSQLRSEGRRFIRAMTQQLGMPLLHLRGDADPYVLADPVERTQRYAPHGRYISIAGAGHFSHEEAPEEVNRHLMRFL
EQVHQLS
>Mature_326_residues
AAPDPSMTRIAGPWRHLDVHANGIRFHVVEAVPSGQPEGPDAATPPMQPALARPLVILLHGFGSFWWSWRHQLCGLTGAR
VVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHPSATLVGHADGGLACWTTALLHSRLVRAIALISSPHPAALRRS
TLTRRDQRHALLPTLLRYQLPIWPERLLTRNNAAEIERLVRARGCAKWLASEDFSQAIDHLRQAIQIPAAAHCALEYQRW
AVRSQLRSEGRRFIRAMTQQLGMPLLHLRGDADPYVLADPVERTQRYAPHGRYISIAGAGHFSHEEAPEEVNRHLMRFLE
QVHQLS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=331, Percent_Identity=28.0966767371601, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI27597073, Length=286, Percent_Identity=24.8251748251748, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI218777840, Length=327, Percent_Identity=27.82874617737, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI13376164, Length=327, Percent_Identity=27.82874617737, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17562298, Length=324, Percent_Identity=26.8518518518519, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17554012, Length=276, Percent_Identity=27.1739130434783, Blast_Score=103, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.3.2.3

Molecular weight: Translated: 36334; Mature: 36203

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPDPSMTRIAGPWRHLDVHANGIRFHVVEAVPSGQPEGPDAATPPMQPALARPLVILL
CCCCCCCCHHHCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
HGFGSFWWSWRHQLCGLTGARVVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHPS
HHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCCC
ATLVGHADGGLACWTTALLHSRLVRAIALISSPHPAALRRSTLTRRDQRHALLPTLLRYQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
LPIWPERLLTRNNAAEIERLVRARGCAKWLASEDFSQAIDHLRQAIQIPAAAHCALEYQR
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
WAVRSQLRSEGRRFIRAMTQQLGMPLLHLRGDADPYVLADPVERTQRYAPHGRYISIAGA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECHHHHHHHCCCCCCEEEEECC
GHFSHEEAPEEVNRHLMRFLEQVHQLS
CCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAPDPSMTRIAGPWRHLDVHANGIRFHVVEAVPSGQPEGPDAATPPMQPALARPLVILL
CCCCCCCHHHCCCCEEEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
HGFGSFWWSWRHQLCGLTGARVVAVDLRGYGGSDKPPRGYDGWTLAGDTAGLIRALGHPS
HHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCCC
ATLVGHADGGLACWTTALLHSRLVRAIALISSPHPAALRRSTLTRRDQRHALLPTLLRYQ
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
LPIWPERLLTRNNAAEIERLVRARGCAKWLASEDFSQAIDHLRQAIQIPAAAHCALEYQR
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
WAVRSQLRSEGRRFIRAMTQQLGMPLLHLRGDADPYVLADPVERTQRYAPHGRYISIAGA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEECHHHHHHHCCCCCCEEEEECC
GHFSHEEAPEEVNRHLMRFLEQVHQLS
CCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; epoxide

Specific reaction: H2O + an epoxide = a glycol

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]