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The map label for this gene is kstD

Identifier: 121639456

GI number: 121639456

Start: 3954261

End: 3955952

Strand: Direct

Name: kstD

Synonym: BCG_3601

Alternate gene names: 121639456

Gene position: 3954261-3955952 (Clockwise)

Preceding gene: 121639451

Following gene: 121639457

Centisome position: 90.39

GC content: 64.72

Gene sequence:

>1692_bases
ATGACTGTGCAGGAGTTCGACGTCGTGGTGGTCGGCAGCGGCGCCGCCGGCATGGTTGCTGCGCTGGTCGCCGCTCACCG
AGGTCTCTCGACGGTAGTCGTCGAGAAGGCCCCGCACTACGGCGGCTCCACCGCACGCTCGGGCGGCGGCGTCTGGATCC
CCAACAACGAGGTCCTCAAGCGCCGCGGCGTTCGAGATACACCGGAGGCGGCACGCACCTATCTGCACGGCATCGTCGGC
GAAATCGTCGAGCCGGAACGCATCGATGCTTACCTCGACCGCGGGCCCGAGATGCTGTCGTTCGTGCTGAAGCACACGCC
GCTGAAGATGTGCTGGGTACCCGGCTACTCCGACTACTACCCCGAGGCTCCGGGCGGCCGCCCGGGCGGACGTTCGATCG
AGCCGAAACCGTTCAACGCGCGCAAGCTTGGTGCCGACATGGCCGGGCTGGAGCCCGCGTATGGCAAGGTTCCGCTCAAT
GTGGTTGTGATGCAGCAGGACTACGTTCGCCTCAATCAGCTCAAACGTCACCCCCGTGGCGTGCTGCGCAGCATGAAGGT
CGGCGCCCGCACGATGTGGGCGAAGGCAACAGGTAAGAACCTGGTCGGCATGGGTCGAGCCCTCATTGGGCCGTTGCGGA
TCGGGTTGCAGCGCGCCGGAGTGCCGGTCGAACTCAACACCGCCTTCACCGATCTTTTCGTCGAAAATGGCGTCGTGTCC
GGGGTATACGTCCGCGATTCCCACGAGGCGGAATCCGCTGAGCCGCAGCTGATCCGGGCTCGCCGCGGCGTGATCCTGGC
CTGTGGTGGTTTCGAGCATAACGAGCAGATGCGAATCAAGTACCAGCGGGCACCCATCACCACCGAGTGGACCGTGGGCG
CCAGCGCCAATACCGGTGACGGCATTCTCGCCGCCGAAAAGCTCGGCGCAGCACTGGATCTGATGGATGACGCTTGGTGG
GGCCCGACGGTACCGCTGGTCGGCAAACCATGGTTCGCGCTCTCGGAGCGCAACTCTCCCGGTTCGATCATCGTCAACAT
GTCAGGCAAGCGATTCATGAACGAATCGATGCCATACGTCGAAGCCTGTCATCATATGTACGGCGGCGAACACGGCCAGG
GGCCCGGACCGGGCGAGAACATTCCGGCGTGGCTGGTGTTCGACCAGCGATACCGGGACCGCTACATCTTCGCGGGACTA
CAACCAGGGCAACGCATTCCGAGCAGGTGGCTGGATTCCGGCGTCATCGTCCAGGCCGATACCCTTGCGGAGCTGGCCGG
CAAGGCCGGTCTACCCGCGGACGAACTCACTGCCACCGTCCAGCGTTTCAACGCATTCGCCCGGTCCGGTGTCGACGAGG
ACTACCACCGCGGGGAAAGTGCCTACGATCGCTACTACGGCGACCCGAGCAACAAGCCCAATCCGAACCTCGGCGAGGTC
GGCCACCCGCCCTATTATGGCGCCAAGATGGTTCCGGGCGACCTGGGGACCAAGGGCGGTATCCGCACCGATGTCAACGG
ACGTGCTCTGCGGGACGACGGCAGCATCATCGACGGCCTTTACGCTGCAGGCAATGTCAGTGCCCCAGTGATGGGACACA
CCTACCCCGGTCCGGGCGGCACGATAGGCCCGGCGATGACGTTCGGGTACCTGGCGGCGCTGCACATTGCCGATCAGGCG
GGAAAGCGCTGA

Upstream 100 bases:

>100_bases
GCTCATCACGGGTGGCATCACGGAGCATTCGGCGAAGTCCCCTCGTAGGCGTGACCGGGCCAGTAGCGCCCGACCCGAGC
AATTCTATAACGTGTTCTAC

Downstream 100 bases:

>100_bases
TATGCCCATCGACTTGGACGTCGCGCTGGGTGCACAGCTACCGCCCGTCGAATTCTCTTGGACCAGTACCGATGTGCAGC
TCTACCAGCTGGGACTGGGC

Product: 3-ketosteroid-delta-1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYGGSTARSGGGVWIPNNEVLKRRGVRDTPEAARTYLHGIVG
EIVEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYYPEAPGGRPGGRSIEPKPFNARKLGADMAGLEPAYGKVPLN
VVVMQQDYVRLNQLKRHPRGVLRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVS
GVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPITTEWTVGASANTGDGILAAEKLGAALDLMDDAWW
GPTVPLVGKPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGPGPGENIPAWLVFDQRYRDRYIFAGL
QPGQRIPSRWLDSGVIVQADTLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPNLGEV
GHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNVSAPVMGHTYPGPGGTIGPAMTFGYLAALHIADQA
GKR

Sequences:

>Translated_563_residues
MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYGGSTARSGGGVWIPNNEVLKRRGVRDTPEAARTYLHGIVG
EIVEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYYPEAPGGRPGGRSIEPKPFNARKLGADMAGLEPAYGKVPLN
VVVMQQDYVRLNQLKRHPRGVLRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVS
GVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPITTEWTVGASANTGDGILAAEKLGAALDLMDDAWW
GPTVPLVGKPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGPGPGENIPAWLVFDQRYRDRYIFAGL
QPGQRIPSRWLDSGVIVQADTLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPNLGEV
GHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNVSAPVMGHTYPGPGGTIGPAMTFGYLAALHIADQA
GKR
>Mature_562_residues
TVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYGGSTARSGGGVWIPNNEVLKRRGVRDTPEAARTYLHGIVGE
IVEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYYPEAPGGRPGGRSIEPKPFNARKLGADMAGLEPAYGKVPLNV
VVMQQDYVRLNQLKRHPRGVLRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVSG
VYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPITTEWTVGASANTGDGILAAEKLGAALDLMDDAWWG
PTVPLVGKPWFALSERNSPGSIIVNMSGKRFMNESMPYVEACHHMYGGEHGQGPGPGENIPAWLVFDQRYRDRYIFAGLQ
PGQRIPSRWLDSGVIVQADTLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPNLGEVG
HPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNVSAPVMGHTYPGPGGTIGPAMTFGYLAALHIADQAG
KR

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): 3O1D2_MYCTU (Q7D5C1)

Other databases:

- EMBL:   AE000516
- RefSeq:   NP_338186.1
- ProteinModelPortal:   Q7D5C1
- EnsemblBacteria:   EBMYCT00000069143
- GeneID:   926568
- GenomeReviews:   AE000516_GR
- KEGG:   mtc:MT3641
- TIGR:   MT3641
- GeneTree:   EBGT00050000015550
- HOGENOM:   HBG335551
- OMA:   AKANTGD
- ProtClustDB:   PRK07843

Pfam domain/function: NA

EC number: =1.3.99.4

Molecular weight: Translated: 60637; Mature: 60506

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYGGSTARSGGGVWIPNNEVLK
CCCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECCCHHHHH
RRGVRDTPEAARTYLHGIVGEIVEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYY
HCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCC
PEAPGGRPGGRSIEPKPFNARKLGADMAGLEPAYGKVPLNVVVMQQDYVRLNQLKRHPRG
CCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHH
VLRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVS
HHHHHHHCHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCEE
GVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPITTEWTVGASANTGD
EEEEECCCCCCCCCHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCCC
GILAAEKLGAALDLMDDAWWGPTVPLVGKPWFALSERNSPGSIIVNMSGKRFMNESMPYV
CEEEHHHHCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCHHHHCCCCHH
EACHHMYGGEHGQGPGPGENIPAWLVFDQRYRDRYIFAGLQPGQRIPSRWLDSGVIVQAD
HHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCHHCHHHHCCCCCEEEHH
TLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPNLGEV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCC
GHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNVSAPVMGHTYPGPGG
CCCCCCCCEECCCCCCCCCCEEECCCCCEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
TIGPAMTFGYLAALHIADQAGKR
CCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVQEFDVVVVGSGAAGMVAALVAAHRGLSTVVVEKAPHYGGSTARSGGGVWIPNNEVLK
CCEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEECCCHHHHH
RRGVRDTPEAARTYLHGIVGEIVEPERIDAYLDRGPEMLSFVLKHTPLKMCWVPGYSDYY
HCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCC
PEAPGGRPGGRSIEPKPFNARKLGADMAGLEPAYGKVPLNVVVMQQDYVRLNQLKRHPRG
CCCCCCCCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHH
VLRSMKVGARTMWAKATGKNLVGMGRALIGPLRIGLQRAGVPVELNTAFTDLFVENGVVS
HHHHHHHCHHHEEHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCEE
GVYVRDSHEAESAEPQLIRARRGVILACGGFEHNEQMRIKYQRAPITTEWTVGASANTGD
EEEEECCCCCCCCCHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCCCC
GILAAEKLGAALDLMDDAWWGPTVPLVGKPWFALSERNSPGSIIVNMSGKRFMNESMPYV
CEEEHHHHCHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCHHHHCCCCHH
EACHHMYGGEHGQGPGPGENIPAWLVFDQRYRDRYIFAGLQPGQRIPSRWLDSGVIVQAD
HHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCHHCHHHHCCCCCEEEHH
TLAELAGKAGLPADELTATVQRFNAFARSGVDEDYHRGESAYDRYYGDPSNKPNPNLGEV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCCC
GHPPYYGAKMVPGDLGTKGGIRTDVNGRALRDDGSIIDGLYAAGNVSAPVMGHTYPGPGG
CCCCCCCCEECCCCCCCCCCEEECCCCCEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
TIGPAMTFGYLAALHIADQAGKR
CCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036