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The map label for this gene is rmlB1

Identifier: 121639384

GI number: 121639384

Start: 3863404

End: 3864399

Strand: Direct

Name: rmlB1

Synonym: BCG_3529

Alternate gene names: 121639384

Gene position: 3863404-3864399 (Clockwise)

Preceding gene: 121639383

Following gene: 121639385

Centisome position: 88.32

GC content: 63.55

Gene sequence:

>996_bases
ATGCGGTTGCTAGTCACCGGTGGCGCGGGATTCATCGGCACGAATTTCGTGCACAGCGCCGTACGTGAGCATCCAGACGA
TGCGGTTACCGTACTCGACGCCCTGACCTACGCCGGCCGGCGCGAGTCGCTGGCCGACGTGGAGGATGCCATCCGGCTGG
TTCAGGGCGATATCACCGACGCCGAGCTGGTTTCGCAGCTGGTGGCCGAGTCCGACGCGGTGGTGCATTTTGCCGCCGAA
TCCCATGTCGACAATGCACTGGACAATCCGGAGCCGTTTCTGCACACCAACGTCATCGGGACCTTCACCATCCTGGAAGC
GGTGCGACGCCACGGTGTGCGCCTGCACCACATCTCCACCGACGAGGTCTACGGCGACTTGGAGCTCGACGACCGGGCGC
GGTTCACCGAATCGACGCCCTATAACCCGTCCAGCCCTTACTCGGCGACCAAGGCGGGCGCAGACATGTTGGTCCGGGCC
TGGGTTCGGTCCTATGGCGTACGCGCGACGATCTCCAACTGCTCCAACAACTACGGGCCGTATCAGCACGTCGAGAAGTT
CATTCCGCGTCAGATCACCAATGTGCTCACCGGGCGGCGGCCCAAGCTCTACGGCGCGGGCGCCAATGTCCGTGACTGGA
TCCACGTCGACGACCACAACAGCGCGGTGCGGCGAATCCTGGACAGAGGCCGCATCGGCCGAACCTACCTGATCAGCTCC
GAGGGCGAGCGTGACAACCTGACCGTGCTGCGCACGCTGCTGCGACTGATGGACCGCGATCCGGACGACTTCGACCACGT
CACCGACCGCGTCGGCCACGACCTGCGCTATGCCATCGACCCGTCCACGCTCTACGACGAATTATGCTGGGCGCCAAAGC
ATACCGATTTCGAGGAGGGCCTGCGGACCACGATCGACTGGTACCGCGACAACGAATCGTGGTGGCGTCCACTAAAAGAC
GCCACGGAGGCCCGCTATCAAGAACGCGGTCAATGA

Upstream 100 bases:

>100_bases
ACTCGCGAAGCCGCTCCGACTGACTTGATCCCGAAACGGAGGGTTGCGAACCCAACTGGTCGCGGCTCCACTCGGTTAAG
GCTCGGTTAGGGTTTGATCC

Downstream 100 bases:

>100_bases
GATGAAAGCACGCGAACTCGACGTCCCCGGCGCCTGGGAGATTACCCCGACCATCCATGTCGATTCCCGCGGACTGTTCT
TCGAATGGCTTACCGATCAT

Product: putative dTDP-glucose 4,6-dehydratase rmlB1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRLLVTGGAGFIGTNFVHSAVREHPDDAVTVLDALTYAGRRESLADVEDAIRLVQGDITDAELVSQLVAESDAVVHFAAE
SHVDNALDNPEPFLHTNVIGTFTILEAVRRHGVRLHHISTDEVYGDLELDDRARFTESTPYNPSSPYSATKAGADMLVRA
WVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGAGANVRDWIHVDDHNSAVRRILDRGRIGRTYLISS
EGERDNLTVLRTLLRLMDRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEGLRTTIDWYRDNESWWRPLKD
ATEARYQERGQ

Sequences:

>Translated_331_residues
MRLLVTGGAGFIGTNFVHSAVREHPDDAVTVLDALTYAGRRESLADVEDAIRLVQGDITDAELVSQLVAESDAVVHFAAE
SHVDNALDNPEPFLHTNVIGTFTILEAVRRHGVRLHHISTDEVYGDLELDDRARFTESTPYNPSSPYSATKAGADMLVRA
WVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGAGANVRDWIHVDDHNSAVRRILDRGRIGRTYLISS
EGERDNLTVLRTLLRLMDRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEGLRTTIDWYRDNESWWRPLKD
ATEARYQERGQ
>Mature_331_residues
MRLLVTGGAGFIGTNFVHSAVREHPDDAVTVLDALTYAGRRESLADVEDAIRLVQGDITDAELVSQLVAESDAVVHFAAE
SHVDNALDNPEPFLHTNVIGTFTILEAVRRHGVRLHHISTDEVYGDLELDDRARFTESTPYNPSSPYSATKAGADMLVRA
WVRSYGVRATISNCSNNYGPYQHVEKFIPRQITNVLTGRRPKLYGAGANVRDWIHVDDHNSAVRRILDRGRIGRTYLISS
EGERDNLTVLRTLLRLMDRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEGLRTTIDWYRDNESWWRPLKD
ATEARYQERGQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=33.033033033033, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI42516563, Length=315, Percent_Identity=26.031746031746, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI4504031, Length=205, Percent_Identity=27.8048780487805, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI48994969, Length=346, Percent_Identity=46.8208092485549, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=44.2577030812325, Blast_Score=291, Evalue=5e-80,
Organism=Escherichia coli, GI1788366, Length=175, Percent_Identity=32, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786974, Length=321, Percent_Identity=25.8566978193146, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=324, Percent_Identity=33.3333333333333, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI115532424, Length=317, Percent_Identity=29.6529968454259, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17539532, Length=321, Percent_Identity=23.0529595015576, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=184, Percent_Identity=29.8913043478261, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=184, Percent_Identity=29.8913043478261, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=184, Percent_Identity=29.8913043478261, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=192, Percent_Identity=29.1666666666667, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=184, Percent_Identity=29.8913043478261, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=185, Percent_Identity=29.1891891891892, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=273, Percent_Identity=26.007326007326, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21356223, Length=221, Percent_Identity=26.2443438914027, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19923002, Length=261, Percent_Identity=30.6513409961686, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24158427, Length=299, Percent_Identity=25.4180602006689, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RMLB_MYCTU (O06329)

Other databases:

- EMBL:   BX842583
- EMBL:   AE000516
- PIR:   E70566
- RefSeq:   NP_217981.1
- RefSeq:   NP_338096.1
- HSSP:   P95780
- ProteinModelPortal:   O06329
- SMR:   O06329
- EnsemblBacteria:   EBMYCT00000003518
- EnsemblBacteria:   EBMYCT00000071206
- GeneID:   887332
- GeneID:   926625
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3570
- KEGG:   mtu:Rv3464
- TIGR:   MT3570
- TubercuList:   Rv3464
- GeneTree:   EBGT00050000015188
- HOGENOM:   HBG755066
- OMA:   EDHVRAI
- ProtClustDB:   CLSK792508
- GO:   GO:0040007
- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01181

Pfam domain/function: PF01370 Epimerase

EC number: =4.2.1.46

Molecular weight: Translated: 37558; Mature: 37558

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: ACT_SITE 121-121 ACT_SITE 122-122 ACT_SITE 147-147 BINDING 81-81 BINDING 96-96 BINDING 176-176 BINDING 177-177 BINDING 211-211 BINDING 246-246

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLVTGGAGFIGTNFVHSAVREHPDDAVTVLDALTYAGRRESLADVEDAIRLVQGDITD
CEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
AELVSQLVAESDAVVHFAAESHVDNALDNPEPFLHTNVIGTFTILEAVRRHGVRLHHIST
HHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEECH
DEVYGDLELDDRARFTESTPYNPSSPYSATKAGADMLVRAWVRSYGVRATISNCSNNYGP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCC
YQHVEKFIPRQITNVLTGRRPKLYGAGANVRDWIHVDDHNSAVRRILDRGRIGRTYLISS
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEECCCHHHHHHHHHCCCCCCEEEECC
EGERDNLTVLRTLLRLMDRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEG
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCHHHH
LRTTIDWYRDNESWWRPLKDATEARYQERGQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLLVTGGAGFIGTNFVHSAVREHPDDAVTVLDALTYAGRRESLADVEDAIRLVQGDITD
CEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCH
AELVSQLVAESDAVVHFAAESHVDNALDNPEPFLHTNVIGTFTILEAVRRHGVRLHHIST
HHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEECH
DEVYGDLELDDRARFTESTPYNPSSPYSATKAGADMLVRAWVRSYGVRATISNCSNNYGP
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHHCCCCCCC
YQHVEKFIPRQITNVLTGRRPKLYGAGANVRDWIHVDDHNSAVRRILDRGRIGRTYLISS
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEECCCHHHHHHHHHCCCCCCEEEECC
EGERDNLTVLRTLLRLMDRDPDDFDHVTDRVGHDLRYAIDPSTLYDELCWAPKHTDFEEG
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCCCHHHH
LRTTIDWYRDNESWWRPLKDATEARYQERGQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036