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The map label for this gene is gatA2 [H]

Identifier: 121639059

GI number: 121639059

Start: 3498052

End: 3499539

Strand: Direct

Name: gatA2 [H]

Synonym: BCG_3199

Alternate gene names: 121639059

Gene position: 3498052-3499539 (Clockwise)

Preceding gene: 121639058

Following gene: 121639062

Centisome position: 79.96

GC content: 68.62

Gene sequence:

>1488_bases
ATGGCCATGTCCGCCAAGGCCTCAGACGATATTGCCTGGCTACCGGCGACCGCTCAACTCGCGGTGCTCGCCGCCAAGAA
GGTGTCCAGCGCGGAGTTAGTCGAGCTGTATCTTTCCCGAATCGACACGTACAACGCGTCGCTCAACGCGATCGTCACCG
TTGACCCCGACGCCGCCCGACGCGTCGCCAAGCGGTCCGATGCGGCACGAGCCCGCGGCGACGAACTCGGCCCGTTGCAT
GGGTTGCCGATCACCGTCAAGGACAGCTATGAGACGGCCGGCATGCGCACGACCTGCGGTCGCCGCGACCTTGCCGACTA
TGTACCCACCCAGGACGCCGAGGCGGTCGCCCGGTTGCGCCGGGCCGGCGCGATCATCATGGGCAAGACAAACATGCCCA
CCGGCAACCAGGACGTCCAGGCCAGCAATCCGGTCTTCGGCCGCACCAACAACCCATGGGACGCCGCGCGCACGTCCGGC
GGCTCGGCCGGCGGCGGGGCGGCCGCCACCGCGGCCGGGCTGACCAGCTTCGACTACGGCTCGGAGATCGGCGGCTCTAC
CAGGATCCCGGCTCATTACTGCGGTCTGTACGGCCACAAATCGACCTGGCGCTCGGTTCCTCTGGTCGGGCACATTCCCA
GCGCACCAGGTAATCCCGGGCGATGGGGGCAAGCCGACATGGCCTGCGCGGGCGTGCAGGTGCGCGGTGCCCGCGACATC
ATCCCCGCACTGGAGGCGACCGTCGGGCCGATGCGGGCGGACGGAGGATTCTCGTATGCGCTCGCTCCGCCACGAGCCGG
CGCGCTCAAAGACTTCCGGGTCGCGGTCTGGGCCGAGGACCCGCATTGCCCAATTGACGCCGACGTGCGTCGGGCCATGG
ATGATGCTGTCGCCGCGCTGCGCGCCGCGGGCGCACACGTCGTTGAGCAGCCCGCCACCATCCCGGTCGATATGGCGGTG
TCGCACAACATCTTCCAGAGTCTGGTGTTCGGCGCCTTCGCTGTCGACCGGTCCACCCTCAGCCCAGCCTCCGCCGCCGC
GCTCGGATTACGCGCGGTTCGGCATCCTCGGGGCGAAGCCGCCAACGCCCTGGGTGCGACGCTACAGAGCCACCGTGCGT
GGTTGTTCGCCGATGCGGCGCGCCACGAAATGCGCGACCGGTGGGCCGGATTCTTCAACGAGTTCGACGTGCTGCTCCTG
CCCGTCACGCCCACCCCCGCGCCGCTCCACCACAACAAGGACCACGACCGGTTGGGCCGCACCATCGACGTCGACGGCGT
CTCACGATCGTACTGGGACCAACTCAAATGGAACGCGCTGGCCAACATCGCCGGCACCCCGGCCACCACCATGCCCATCA
CCACCACAGCTACCGGACTCCCGATCGGCATCCAGGCGATGGGGCCCGCGGGCGGAGACCGCACCACCGTAGAGTTCGCC
GCCCTGCTCACCGAAGTCCTAGGCGGCTTCCGCGTTCCCCCTCTTTAG

Upstream 100 bases:

>100_bases
TGCTCGCCGACGAGATGAGCCGTCAGGTGCGCGCGTCGCTGAATGTCCCTGCGCGGGAACGTATCGCGCGGTTGATGGGT
AACTGAGTCCGAAAGTCGAT

Downstream 100 bases:

>100_bases
GAACGCTCGGGCAGGGCCGCAATAACCTCGGCGAGCCGATCGGGCTGCTCCGCTGTCGTCAGGTGGCCGCCCGCAAGCTC
GGTGATTTCCACCGAATCCG

Product: amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MAMSAKASDDIAWLPATAQLAVLAAKKVSSAELVELYLSRIDTYNASLNAIVTVDPDAARRVAKRSDAARARGDELGPLH
GLPITVKDSYETAGMRTTCGRRDLADYVPTQDAEAVARLRRAGAIIMGKTNMPTGNQDVQASNPVFGRTNNPWDAARTSG
GSAGGGAAATAAGLTSFDYGSEIGGSTRIPAHYCGLYGHKSTWRSVPLVGHIPSAPGNPGRWGQADMACAGVQVRGARDI
IPALEATVGPMRADGGFSYALAPPRAGALKDFRVAVWAEDPHCPIDADVRRAMDDAVAALRAAGAHVVEQPATIPVDMAV
SHNIFQSLVFGAFAVDRSTLSPASAAALGLRAVRHPRGEAANALGATLQSHRAWLFADAARHEMRDRWAGFFNEFDVLLL
PVTPTPAPLHHNKDHDRLGRTIDVDGVSRSYWDQLKWNALANIAGTPATTMPITTTATGLPIGIQAMGPAGGDRTTVEFA
ALLTEVLGGFRVPPL

Sequences:

>Translated_495_residues
MAMSAKASDDIAWLPATAQLAVLAAKKVSSAELVELYLSRIDTYNASLNAIVTVDPDAARRVAKRSDAARARGDELGPLH
GLPITVKDSYETAGMRTTCGRRDLADYVPTQDAEAVARLRRAGAIIMGKTNMPTGNQDVQASNPVFGRTNNPWDAARTSG
GSAGGGAAATAAGLTSFDYGSEIGGSTRIPAHYCGLYGHKSTWRSVPLVGHIPSAPGNPGRWGQADMACAGVQVRGARDI
IPALEATVGPMRADGGFSYALAPPRAGALKDFRVAVWAEDPHCPIDADVRRAMDDAVAALRAAGAHVVEQPATIPVDMAV
SHNIFQSLVFGAFAVDRSTLSPASAAALGLRAVRHPRGEAANALGATLQSHRAWLFADAARHEMRDRWAGFFNEFDVLLL
PVTPTPAPLHHNKDHDRLGRTIDVDGVSRSYWDQLKWNALANIAGTPATTMPITTTATGLPIGIQAMGPAGGDRTTVEFA
ALLTEVLGGFRVPPL
>Mature_494_residues
AMSAKASDDIAWLPATAQLAVLAAKKVSSAELVELYLSRIDTYNASLNAIVTVDPDAARRVAKRSDAARARGDELGPLHG
LPITVKDSYETAGMRTTCGRRDLADYVPTQDAEAVARLRRAGAIIMGKTNMPTGNQDVQASNPVFGRTNNPWDAARTSGG
SAGGGAAATAAGLTSFDYGSEIGGSTRIPAHYCGLYGHKSTWRSVPLVGHIPSAPGNPGRWGQADMACAGVQVRGARDII
PALEATVGPMRADGGFSYALAPPRAGALKDFRVAVWAEDPHCPIDADVRRAMDDAVAALRAAGAHVVEQPATIPVDMAVS
HNIFQSLVFGAFAVDRSTLSPASAAALGLRAVRHPRGEAANALGATLQSHRAWLFADAARHEMRDRWAGFFNEFDVLLLP
VTPTPAPLHHNKDHDRLGRTIDVDGVSRSYWDQLKWNALANIAGTPATTMPITTTATGLPIGIQAMGPAGGDRTTVEFAA
LLTEVLGGFRVPPL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=506, Percent_Identity=27.4703557312253, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI222831590, Length=502, Percent_Identity=27.6892430278884, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI166795287, Length=181, Percent_Identity=30.3867403314917, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=515, Percent_Identity=27.7669902912621, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17556264, Length=291, Percent_Identity=32.3024054982818, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17543272, Length=448, Percent_Identity=27.2321428571429, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=481, Percent_Identity=25.1559251559252, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=140, Percent_Identity=36.4285714285714, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=307, Percent_Identity=29.6416938110749, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=306, Percent_Identity=29.4117647058824, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538254, Length=198, Percent_Identity=31.8181818181818, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=189, Percent_Identity=32.2751322751323, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=414, Percent_Identity=23.6714975845411, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356731, Length=491, Percent_Identity=27.4949083503055, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24644968, Length=502, Percent_Identity=28.6852589641434, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=28.2, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI24648435, Length=502, Percent_Identity=25.4980079681275, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24648437, Length=502, Percent_Identity=25.4980079681275, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI45550774, Length=502, Percent_Identity=25.4980079681275, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24652985, Length=525, Percent_Identity=27.047619047619, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19922090, Length=525, Percent_Identity=27.047619047619, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24652981, Length=525, Percent_Identity=27.047619047619, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24652983, Length=525, Percent_Identity=27.047619047619, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24648113, Length=474, Percent_Identity=30.379746835443, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648441, Length=435, Percent_Identity=25.0574712643678, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648439, Length=435, Percent_Identity=25.0574712643678, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 52082; Mature: 51951

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSAKASDDIAWLPATAQLAVLAAKKVSSAELVELYLSRIDTYNASLNAIVTVDPDAAR
CCCCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
RVAKRSDAARARGDELGPLHGLPITVKDSYETAGMRTTCGRRDLADYVPTQDAEAVARLR
HHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHH
RAGAIIMGKTNMPTGNQDVQASNPVFGRTNNPWDAARTSGGSAGGGAAATAAGLTSFDYG
HCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCC
SEIGGSTRIPAHYCGLYGHKSTWRSVPLVGHIPSAPGNPGRWGQADMACAGVQVRGARDI
CCCCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEECEEECCCHHH
IPALEATVGPMRADGGFSYALAPPRAGALKDFRVAVWAEDPHCPIDADVRRAMDDAVAAL
HHHHHHHCCCEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
RAAGAHVVEQPATIPVDMAVSHNIFQSLVFGAFAVDRSTLSPASAAALGLRAVRHPRGEA
HHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHH
ANALGATLQSHRAWLFADAARHEMRDRWAGFFNEFDVLLLPVTPTPAPLHHNKDHDRLGR
HHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCCC
TIDVDGVSRSYWDQLKWNALANIAGTPATTMPITTTATGLPIGIQAMGPAGGDRTTVEFA
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHH
ALLTEVLGGFRVPPL
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
AMSAKASDDIAWLPATAQLAVLAAKKVSSAELVELYLSRIDTYNASLNAIVTVDPDAAR
CCCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
RVAKRSDAARARGDELGPLHGLPITVKDSYETAGMRTTCGRRDLADYVPTQDAEAVARLR
HHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHH
RAGAIIMGKTNMPTGNQDVQASNPVFGRTNNPWDAARTSGGSAGGGAAATAAGLTSFDYG
HCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCC
SEIGGSTRIPAHYCGLYGHKSTWRSVPLVGHIPSAPGNPGRWGQADMACAGVQVRGARDI
CCCCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEECEEECCCHHH
IPALEATVGPMRADGGFSYALAPPRAGALKDFRVAVWAEDPHCPIDADVRRAMDDAVAAL
HHHHHHHCCCEECCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
RAAGAHVVEQPATIPVDMAVSHNIFQSLVFGAFAVDRSTLSPASAAALGLRAVRHPRGEA
HHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHH
ANALGATLQSHRAWLFADAARHEMRDRWAGFFNEFDVLLLPVTPTPAPLHHNKDHDRLGR
HHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHCCC
TIDVDGVSRSYWDQLKWNALANIAGTPATTMPITTTATGLPIGIQAMGPAGGDRTTVEFA
EEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHH
ALLTEVLGGFRVPPL
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]