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The map label for this gene is entA [C]

Identifier: 121638145

GI number: 121638145

Start: 2514093

End: 2515046

Strand: Direct

Name: entA [C]

Synonym: BCG_2280

Alternate gene names: 121638145

Gene position: 2514093-2515046 (Clockwise)

Preceding gene: 121638143

Following gene: 121638147

Centisome position: 57.47

GC content: 66.77

Gene sequence:

>954_bases
ATGGCCAAGGATCTGGTCGCCACGGTGCCCGATCTTTCCGGGAAGCTGGCAATCATCACCGGCGCCAACAGCGGTCTAGG
CTTCGGGCTGGCCCGGCGGCTGTCGGCGGCTGGCGCCGACGTAATCATGGCGATCCGCAATCGCGCCAAGGGCGAGGCGG
CGGTCGAGGAAATCCGGACCGCGGTTCCGGATGCGAAGCTGACCATCAAGGCCCTCGACCTGTCATCGTTGGCGTCCGTC
GCCGCGTTGGGGGAACAGCTCATGGCTGACGGGCGGCCGATCGACCTGCTGATCAACAACGCCGGCGTCATGACCCCACC
GGAACGCGTTACCACTGCCGACGGCTTCGAATTGCAGTTCGGCAGCAACCATCTCGGACACTTCGCGCTAACCGCACACC
TGCTGCCGCTGTTGCGCGCGGCACAGCGCGCGAGGGTCGTCTCGTTGAGCAGCTTGGCGGCCCGCCGCGGCCGCATCCAC
TTCGACGACCTACAGTTCGAGAGGTCGTACGCCCCGATGACGGCCTATGGCCAGTCGAAGCTGGCGGTCTTGATGTTCGC
CCGCGAGCTGGACCGCCGCAGCCGCGCGGCCGGCTGGGGCATCATCTCCAATGCCGCGCATCCTGGCTTGACCAAGACCA
ACCTGCAGATCGCGGGACCGTCCCATGGCCGCGACAAGCCGGCGCTGATGGAACGCTTGTACAAGACGTCCTGGCGTTTC
GCACCGTTCCTCTGGCAGGAGATCGAAGAGGGGATCTTGCCCGCGCTGTATGCAGCCGCCACCCCGCAAGCCGACGGTGG
CGCGTTCTATGGCCCCCGCGGCCGCTACGAGGTCGCCGGCGGTGGTGTGCGAGAGGCCAAGGTTCCCGCAGCCGCCCGCA
ACGACGCCGATAGCAAGCGACTTTGGGAGGTCTCCGAGCAGCTCACCGGTGTCAGCTACCCGAAATCGCGCTGA

Upstream 100 bases:

>100_bases
GCGCAACGCCATGCCGATCAGCATGCCGGCCACATTCGCCTGCGTCGAGGAAAAGAGCAGACTAAGACCGGCAGTCCCCG
CCAGAAAGGGAGTGATTTGC

Downstream 100 bases:

>100_bases
ACTGCCCGATCCCGGGAACCTGAGGTATTCCGGGGGGGAGCTGCGGAATCTCCGGAATCGGTGGGATCGGCGGGATCGGT
GGAGGGCTGGGGGACGTGGT

Product: short chain dehydrogenase

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MAKDLVATVPDLSGKLAIITGANSGLGFGLARRLSAAGADVIMAIRNRAKGEAAVEEIRTAVPDAKLTIKALDLSSLASV
AALGEQLMADGRPIDLLINNAGVMTPPERVTTADGFELQFGSNHLGHFALTAHLLPLLRAAQRARVVSLSSLAARRGRIH
FDDLQFERSYAPMTAYGQSKLAVLMFARELDRRSRAAGWGIISNAAHPGLTKTNLQIAGPSHGRDKPALMERLYKTSWRF
APFLWQEIEEGILPALYAAATPQADGGAFYGPRGRYEVAGGGVREAKVPAAARNDADSKRLWEVSEQLTGVSYPKSR

Sequences:

>Translated_317_residues
MAKDLVATVPDLSGKLAIITGANSGLGFGLARRLSAAGADVIMAIRNRAKGEAAVEEIRTAVPDAKLTIKALDLSSLASV
AALGEQLMADGRPIDLLINNAGVMTPPERVTTADGFELQFGSNHLGHFALTAHLLPLLRAAQRARVVSLSSLAARRGRIH
FDDLQFERSYAPMTAYGQSKLAVLMFARELDRRSRAAGWGIISNAAHPGLTKTNLQIAGPSHGRDKPALMERLYKTSWRF
APFLWQEIEEGILPALYAAATPQADGGAFYGPRGRYEVAGGGVREAKVPAAARNDADSKRLWEVSEQLTGVSYPKSR
>Mature_316_residues
AKDLVATVPDLSGKLAIITGANSGLGFGLARRLSAAGADVIMAIRNRAKGEAAVEEIRTAVPDAKLTIKALDLSSLASVA
ALGEQLMADGRPIDLLINNAGVMTPPERVTTADGFELQFGSNHLGHFALTAHLLPLLRAAQRARVVSLSSLAARRGRIHF
DDLQFERSYAPMTAYGQSKLAVLMFARELDRRSRAAGWGIISNAAHPGLTKTNLQIAGPSHGRDKPALMERLYKTSWRFA
PFLWQEIEEGILPALYAAATPQADGGAFYGPRGRYEVAGGGVREAKVPAAARNDADSKRLWEVSEQLTGVSYPKSR

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI10190746, Length=316, Percent_Identity=37.6582278481013, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI186928839, Length=316, Percent_Identity=37.3417721518987, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI225579078, Length=318, Percent_Identity=38.3647798742138, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI166795268, Length=314, Percent_Identity=35.3503184713376, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI193804850, Length=306, Percent_Identity=33.6601307189542, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI19923931, Length=275, Percent_Identity=37.8181818181818, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI7706523, Length=314, Percent_Identity=32.1656050955414, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI146231950, Length=314, Percent_Identity=35.3503184713376, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI7705421, Length=233, Percent_Identity=30.4721030042918, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4502601, Length=236, Percent_Identity=27.9661016949153, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI19743808, Length=240, Percent_Identity=30.4166666666667, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI150247226, Length=148, Percent_Identity=37.1621621621622, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI20149619, Length=212, Percent_Identity=31.6037735849057, Blast_Score=66, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193207620, Length=314, Percent_Identity=34.0764331210191, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17532805, Length=315, Percent_Identity=31.1111111111111, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17558006, Length=205, Percent_Identity=35.1219512195122, Blast_Score=120, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI71992402, Length=208, Percent_Identity=38.9423076923077, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17532791, Length=316, Percent_Identity=27.2151898734177, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32565532, Length=208, Percent_Identity=35.5769230769231, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17505416, Length=322, Percent_Identity=26.7080745341615, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17535059, Length=208, Percent_Identity=35.5769230769231, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI72001364, Length=315, Percent_Identity=29.8412698412698, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324820, Length=327, Percent_Identity=27.82874617737, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323882, Length=211, Percent_Identity=28.436018957346, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573296, Length=313, Percent_Identity=38.0191693290735, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI19921754, Length=318, Percent_Identity=35.2201257861635, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI22024069, Length=306, Percent_Identity=35.6209150326797, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24586328, Length=303, Percent_Identity=35.3135313531353, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI161076371, Length=255, Percent_Identity=38.4313725490196, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI45554248, Length=301, Percent_Identity=36.2126245847176, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24640117, Length=301, Percent_Identity=36.2126245847176, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24656154, Length=317, Percent_Identity=33.4384858044164, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI19922628, Length=317, Percent_Identity=33.4384858044164, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI20129347, Length=207, Percent_Identity=39.1304347826087, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553409, Length=313, Percent_Identity=28.4345047923323, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647948, Length=313, Percent_Identity=28.4345047923323, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647946, Length=317, Percent_Identity=28.391167192429, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24647931, Length=275, Percent_Identity=31.6363636363636, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21358495, Length=226, Percent_Identity=31.4159292035398, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 33966; Mature: 33835

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKDLVATVPDLSGKLAIITGANSGLGFGLARRLSAAGADVIMAIRNRAKGEAAVEEIRT
CCCCCEECCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHH
AVPDAKLTIKALDLSSLASVAALGEQLMADGRPIDLLINNAGVMTPPERVTTADGFELQF
HCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCEEEEE
GSNHLGHFALTAHLLPLLRAAQRARVVSLSSLAARRGRIHFDDLQFERSYAPMTAYGQSK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHCCCCCCCCCCCCH
LAVLMFARELDRRSRAAGWGIISNAAHPGLTKTNLQIAGPSHGRDKPALMERLYKTSWRF
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCH
APFLWQEIEEGILPALYAAATPQADGGAFYGPRGRYEVAGGGVREAKVPAAARNDADSKR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHH
LWEVSEQLTGVSYPKSR
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AKDLVATVPDLSGKLAIITGANSGLGFGLARRLSAAGADVIMAIRNRAKGEAAVEEIRT
CCCCEECCCCCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHH
AVPDAKLTIKALDLSSLASVAALGEQLMADGRPIDLLINNAGVMTPPERVTTADGFELQF
HCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHCCCCCCEEEEE
GSNHLGHFALTAHLLPLLRAAQRARVVSLSSLAARRGRIHFDDLQFERSYAPMTAYGQSK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHCCCCCCCCCCCCH
LAVLMFARELDRRSRAAGWGIISNAAHPGLTKTNLQIAGPSHGRDKPALMERLYKTSWRF
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCH
APFLWQEIEEGILPALYAAATPQADGGAFYGPRGRYEVAGGGVREAKVPAAARNDADSKR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHH
LWEVSEQLTGVSYPKSR
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]