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The map label for this gene is asnB

Identifier: 121638082

GI number: 121638082

Start: 2443641

End: 2445599

Strand: Direct

Name: asnB

Synonym: BCG_2217

Alternate gene names: 121638082

Gene position: 2443641-2445599 (Clockwise)

Preceding gene: 121638077

Following gene: 121638084

Centisome position: 55.86

GC content: 66.11

Gene sequence:

>1959_bases
GTGTGTGGACTGCTGGCCTTCGTCGCGGCCCCGGCCGGTGCTGCGGGGCCCGAAGGTGCCGACGCTGCCAGCGCCATCGC
CCGCGCATCGCATTTGATGCGCCACCGCGGGCCCGATGAATCGGGCACCTGGCACGCCGTCGATGGCGCCTCCGGAGGCG
TCGTGTTCGGGTTCAACCGACTGTCCATCATCGACATCGCGCACTCGCATCAGCCGCTGCGGTGGGGGCCGCCGGAGGCT
CCGGACCGCTACGTGCTGGTGTTCAACGGCGAGATCTACAACTACTTGGAGCTGCGTGACGAGCTGCGCACCCAGCACGG
CGCTGTGTTCGCCACCGACGGCGACGGTGAGGCGATCCTCGCCGGCTATCACCACTGGGGCACCGAGGTGCTGCAGCGGT
TGCGCGGCATGTTCGCATTCGCGCTGTGGGACACCGTCACCCGCGAATTGTTCTGCGCGCGAGATCCGTTCGGCATCAAG
CCGTTGTTTATCGCCACCGGAGCCGGCGGCACGGCGGTGGCCAGTGAGAAGAAATGCCTGCTGGACCTCGTCGAGTTGGT
GGGGTTCGACACCGAGATCGACCATCGGGCGTTGCAGCACTACACCGTCCTGCAGTACGTGCCGGAACCCGAGACACTGC
ACCGTGGGGTACGTCGGCTGGAATCAGGCTGCTTCGCCCGGATCCGTGCCGACCAGCTCGCGCCGGTGATCACCCGTTAT
TTCGTGCCGCGATTTGCGGCCAGTCCGATCACCAACGACAACGACCAGGCCCGCTATGACGAGATCACGGCAGTGCTTGA
GGACTCGGTGGCCAAGCATATGCGCGCCGATGTCACCGTCGGCGCGTTTCTGTCCGGGGGTATCGACTCCACGGCCATCG
CGGCGCTGGCCATCCGGCACAATCCGCGGCTGATCACCTTCACCACCGGTTTCGAGCGCGAGGGCTTCTCCGAGATCGAC
GTCGCGGTGGCTTCGGCAGAGGCCATCGGTGCCCGTCACATCGCCAAGGTGGTCAGCGCCGACGAGTTCGTCGCCGCCCT
GCCCGAGATCGTCTGGTACCTCGACGAGCCGGTCGCTGACCCAGCGCTGGTACCGTTGTTCTTCGTCGCCCGCGAGGCCC
GAAAGCACGTCAAAGTGGTGTTGTCGGGCGAAGGCGCCGACGAACTGTTCGGCGGCTACACAATCTATCGAGAACCGCTG
TCGTTGAGGCCGTTTGACTACCTGCCCAAGCCACTGCGCCGGTCGATGGGAAAAGTTTCCAAGCCACTGCCGGAGGGCAT
GCGCGGCAAGAGTCTGCTGCACCGCGGATCGCTGACACTCGAAGAGCGCTACTACGGCAATGCCCGCAGTTTCTCCGGCG
CGCAGCTGCGCGAAGTACTGCCCGGGTTCCGGCCGGACTGGACCCACACAGATGTCACGGCGCCGGTCTACGCCGAATCG
GCCGGCTGGGATCCGGTGGCGCGAATGCAGCACATCGACCTGTTCACCTGGCTGCGCGGCGACATTCTGGTCAAGGCCGA
CAAGATAACGATGGCCAACTCCCTGGAGCTGCGGGTGCCGTTCCTGGACCCGGAGGTTTTCGCGGTGGCCTCCCGGTTGC
CGGCGGGCGCCAAGATCACCCGTACCACCACCAAGTACGCGCTGCGGCGCGCGCTGGAGCCTATTGTGCCCGCACACGTG
CTGCACCGGCCCAAGCTCGGGTTCCCGGTCCCGATCCGGCATTGGCTGCGTGCCGGCGAGCTGCTGGAGTGGGCGTATGC
GACGGTGGGCTCGTCGCAGGCCGGTCACTTGGTTGACATCGCCGCCGTGTATCGCATGCTCGACGAGCACCGGTGCGGCA
GCAGCGACCACAGCCGCCGGCTGTGGACCATGCTGATCTTTATGCTGTGGCACGCGATCTTCGTCGAGCACAGCGTGGTG
CCCCAGATCAGCGAGCCGCAGTACCCCGTCCAGTTGTAA

Upstream 100 bases:

>100_bases
GAGGAGCCGCGCCATCAGCCCCGCGGGCGACTGCCTGGTCGTCGCGACCCGCCGGACGAGGCATCCACAAGAGTCGCCAA
GTGGGGCATACTGGGGCGCC

Downstream 100 bases:

>100_bases
CCGCCCCTTCGCGAGCAGACGCGGAATCGCATCGGCGGGGCCCACACGGTGCGATTCCGCGTCTGCTCGGCGGTGCCGCG
GCTAGGCCAAGCCGCGGCTA

Product: putative asparagine synthetase AsnB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MCGLLAFVAAPAGAAGPEGADAASAIARASHLMRHRGPDESGTWHAVDGASGGVVFGFNRLSIIDIAHSHQPLRWGPPEA
PDRYVLVFNGEIYNYLELRDELRTQHGAVFATDGDGEAILAGYHHWGTEVLQRLRGMFAFALWDTVTRELFCARDPFGIK
PLFIATGAGGTAVASEKKCLLDLVELVGFDTEIDHRALQHYTVLQYVPEPETLHRGVRRLESGCFARIRADQLAPVITRY
FVPRFAASPITNDNDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAIRHNPRLITFTTGFEREGFSEID
VAVASAEAIGARHIAKVVSADEFVAALPEIVWYLDEPVADPALVPLFFVAREARKHVKVVLSGEGADELFGGYTIYREPL
SLRPFDYLPKPLRRSMGKVSKPLPEGMRGKSLLHRGSLTLEERYYGNARSFSGAQLREVLPGFRPDWTHTDVTAPVYAES
AGWDPVARMQHIDLFTWLRGDILVKADKITMANSLELRVPFLDPEVFAVASRLPAGAKITRTTTKYALRRALEPIVPAHV
LHRPKLGFPVPIRHWLRAGELLEWAYATVGSSQAGHLVDIAAVYRMLDEHRCGSSDHSRRLWTMLIFMLWHAIFVEHSVV
PQISEPQYPVQL

Sequences:

>Translated_652_residues
MCGLLAFVAAPAGAAGPEGADAASAIARASHLMRHRGPDESGTWHAVDGASGGVVFGFNRLSIIDIAHSHQPLRWGPPEA
PDRYVLVFNGEIYNYLELRDELRTQHGAVFATDGDGEAILAGYHHWGTEVLQRLRGMFAFALWDTVTRELFCARDPFGIK
PLFIATGAGGTAVASEKKCLLDLVELVGFDTEIDHRALQHYTVLQYVPEPETLHRGVRRLESGCFARIRADQLAPVITRY
FVPRFAASPITNDNDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAIRHNPRLITFTTGFEREGFSEID
VAVASAEAIGARHIAKVVSADEFVAALPEIVWYLDEPVADPALVPLFFVAREARKHVKVVLSGEGADELFGGYTIYREPL
SLRPFDYLPKPLRRSMGKVSKPLPEGMRGKSLLHRGSLTLEERYYGNARSFSGAQLREVLPGFRPDWTHTDVTAPVYAES
AGWDPVARMQHIDLFTWLRGDILVKADKITMANSLELRVPFLDPEVFAVASRLPAGAKITRTTTKYALRRALEPIVPAHV
LHRPKLGFPVPIRHWLRAGELLEWAYATVGSSQAGHLVDIAAVYRMLDEHRCGSSDHSRRLWTMLIFMLWHAIFVEHSVV
PQISEPQYPVQL
>Mature_652_residues
MCGLLAFVAAPAGAAGPEGADAASAIARASHLMRHRGPDESGTWHAVDGASGGVVFGFNRLSIIDIAHSHQPLRWGPPEA
PDRYVLVFNGEIYNYLELRDELRTQHGAVFATDGDGEAILAGYHHWGTEVLQRLRGMFAFALWDTVTRELFCARDPFGIK
PLFIATGAGGTAVASEKKCLLDLVELVGFDTEIDHRALQHYTVLQYVPEPETLHRGVRRLESGCFARIRADQLAPVITRY
FVPRFAASPITNDNDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAIRHNPRLITFTTGFEREGFSEID
VAVASAEAIGARHIAKVVSADEFVAALPEIVWYLDEPVADPALVPLFFVAREARKHVKVVLSGEGADELFGGYTIYREPL
SLRPFDYLPKPLRRSMGKVSKPLPEGMRGKSLLHRGSLTLEERYYGNARSFSGAQLREVLPGFRPDWTHTDVTAPVYAES
AGWDPVARMQHIDLFTWLRGDILVKADKITMANSLELRVPFLDPEVFAVASRLPAGAKITRTTTKYALRRALEPIVPAHV
LHRPKLGFPVPIRHWLRAGELLEWAYATVGSSQAGHLVDIAAVYRMLDEHRCGSSDHSRRLWTMLIFMLWHAIFVEHSVV
PQISEPQYPVQL

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain

Homologues:

Organism=Homo sapiens, GI168229248, Length=410, Percent_Identity=28.2926829268293, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI168229252, Length=410, Percent_Identity=28.2926829268293, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI168229250, Length=410, Percent_Identity=28.2926829268293, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI296010848, Length=376, Percent_Identity=29.2553191489362, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI296010852, Length=301, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI296010850, Length=301, Percent_Identity=28.5714285714286, Blast_Score=102, Evalue=9e-22,
Organism=Escherichia coli, GI1786889, Length=388, Percent_Identity=29.8969072164948, Blast_Score=148, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71993933, Length=420, Percent_Identity=29.7619047619048, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25147557, Length=420, Percent_Identity=29.7619047619048, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17560178, Length=437, Percent_Identity=28.3752860411899, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25147560, Length=346, Percent_Identity=28.6127167630058, Blast_Score=96, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=420, Percent_Identity=28.0952380952381, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=419, Percent_Identity=28.4009546539379, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI45553209, Length=388, Percent_Identity=31.4432989690722, Blast_Score=161, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ASNH_MYCBO (P64248)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855873.1
- ProteinModelPortal:   P64248
- SMR:   P64248
- EnsemblBacteria:   EBMYCT00000016287
- GeneID:   1091346
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2224
- GeneTree:   EBGT00050000016153
- HOGENOM:   HBG752912
- OMA:   IVWYLDD
- ProtClustDB:   CLSK2484247
- BioCyc:   MBOV233413:MB2224-MONOMER
- BRENDA:   6.3.5.4
- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR01536

Pfam domain/function: PF00733 Asn_synthase

EC number: =6.3.5.4

Molecular weight: Translated: 72150; Mature: 72150

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS51278 GATASE_TYPE_2

Important sites: ACT_SITE 2-2

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLLAFVAAPAGAAGPEGADAASAIARASHLMRHRGPDESGTWHAVDGASGGVVFGFNR
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEECC
LSIIDIAHSHQPLRWGPPEAPDRYVLVFNGEIYNYLELRDELRTQHGAVFATDGDGEAIL
EEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCEEE
AGYHHWGTEVLQRLRGMFAFALWDTVTRELFCARDPFGIKPLFIATGAGGTAVASEKKCL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHH
LDLVELVGFDTEIDHRALQHYTVLQYVPEPETLHRGVRRLESGCFARIRADQLAPVITRY
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHH
FVPRFAASPITNDNDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAIRH
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCHHHHHHEEEEC
NPRLITFTTGFEREGFSEIDVAVASAEAIGARHIAKVVSADEFVAALPEIVWYLDEPVAD
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PALVPLFFVAREARKHVKVVLSGEGADELFGGYTIYREPLSLRPFDYLPKPLRRSMGKVS
CHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHC
KPLPEGMRGKSLLHRGSLTLEERYYGNARSFSGAQLREVLPGFRPDWTHTDVTAPVYAES
CCCCCCCCCHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEECC
AGWDPVARMQHIDLFTWLRGDILVKADKITMANSLELRVPFLDPEVFAVASRLPAGAKIT
CCCCHHHHHHHHHHHHHHCCCEEEEECCEEEECCEEEEEECCCHHHHHHHHHCCCCCEEE
RTTTKYALRRALEPIVPAHVLHRPKLGFPVPIRHWLRAGELLEWAYATVGSSQAGHLVDI
HHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AAVYRMLDEHRCGSSDHSRRLWTMLIFMLWHAIFVEHSVVPQISEPQYPVQL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGLLAFVAAPAGAAGPEGADAASAIARASHLMRHRGPDESGTWHAVDGASGGVVFGFNR
CCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEECC
LSIIDIAHSHQPLRWGPPEAPDRYVLVFNGEIYNYLELRDELRTQHGAVFATDGDGEAIL
EEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCEEE
AGYHHWGTEVLQRLRGMFAFALWDTVTRELFCARDPFGIKPLFIATGAGGTAVASEKKCL
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHH
LDLVELVGFDTEIDHRALQHYTVLQYVPEPETLHRGVRRLESGCFARIRADQLAPVITRY
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHH
FVPRFAASPITNDNDQARYDEITAVLEDSVAKHMRADVTVGAFLSGGIDSTAIAALAIRH
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCCHHHHHHEEEEC
NPRLITFTTGFEREGFSEIDVAVASAEAIGARHIAKVVSADEFVAALPEIVWYLDEPVAD
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PALVPLFFVAREARKHVKVVLSGEGADELFGGYTIYREPLSLRPFDYLPKPLRRSMGKVS
CHHHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHC
KPLPEGMRGKSLLHRGSLTLEERYYGNARSFSGAQLREVLPGFRPDWTHTDVTAPVYAES
CCCCCCCCCHHHHHCCCCEEHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEECC
AGWDPVARMQHIDLFTWLRGDILVKADKITMANSLELRVPFLDPEVFAVASRLPAGAKIT
CCCCHHHHHHHHHHHHHHCCCEEEEECCEEEECCEEEEEECCCHHHHHHHHHCCCCCEEE
RTTTKYALRRALEPIVPAHVLHRPKLGFPVPIRHWLRAGELLEWAYATVGSSQAGHLVDI
HHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AAVYRMLDEHRCGSSDHSRRLWTMLIFMLWHAIFVEHSVVPQISEPQYPVQL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972