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The map label for this gene is pfkB

Identifier: 121637915

GI number: 121637915

Start: 2259057

End: 2260076

Strand: Reverse

Name: pfkB

Synonym: BCG_2048c

Alternate gene names: 121637915

Gene position: 2260076-2259057 (Counterclockwise)

Preceding gene: 121637916

Following gene: 121637914

Centisome position: 51.66

GC content: 64.8

Gene sequence:

>1020_bases
ATGACGGAGCCAGCGGCGTGGGACGAAGGCAAGCCGCGAATCATCACTTTGACCATGAACCCCGCCTTGGACATCACGAC
GAGCGTCGACGTGGTGCGCCCGACCGAGAAAATGCGTTGTGGCGCACCTCGCTACGATCCCGGCGGCGGCGGTATCAATG
TCGCCCGCATTGTGCATGTCCTCGGCGGTTGCTCGACAGCACTGTTCCCGGCCGGCGGGTCGACCGGGAGCCTGCTGATG
GCGCTGCTCGGTGATGCGGGAGTGCCATTTCGCGTCATTCCGATCGCGGCCTCGACGCGGGAGAGCTTCACGGTCAACGA
GTCCAGGACCGCCAAGCAGTATCGTTTCGTGCTTCCGGGGCCGTCGCTGACCGTCGCGGAGCAGGAGCAATGCCTCGACG
AACTGCGCGGTGCGGCGGCTTCGGCCGCCTTTGTGGTGGCCAGTGGCAGCCTGCCGCCAGGTGTGGCTGCCGACTACTAT
CAGCGGGTTGCCGACATCTGCCGCCGATCGAGCACTCCGCTGATCCTGGATACATCTGGTGGCGGGTTGCAGCACATTTC
GTCCGGGGTGTTTCTTCTCAAGGCGAGCGTGCGGGAACTGCGCGAGTGCGTCGGATCCGAACTGCTGACCGAGCCCGAAC
AACTGGCCGCCGCACACGAACTCATTGACCGTGGGCGCGCCGAGGTCGTGGTGGTCTCGCTTGGATCTCAGGGCGCGCTA
TTGGCCACACGACATGCGAGCCATCGATTTTCGTCGATTCCGATGACCGCGGTTAGCGGTGTCGGCGCCGGCGACGCGAT
GGTGGCCGCGATTACCGTGGGCCTCAGCCGTGGCTGGTCGCTCATCAAGTCCGTTCGCTTGGGAAACGCGGCAGGTGCAG
CCATGCTGCTGACGCCAGGCACCGCGGCCTGCAATCGCGACGATGTGGAGAGGTTCTTCGAGCTGGCGGCCGAACCCACC
GAAGTCGGGCAGGATCAATACGTTTGGCACCCGATCGTTAACCCGGAAGCCTCGCCATGA

Upstream 100 bases:

>100_bases
CAGACCCGTGCCCTGGAACCTCTCGAGGTGACGAGCCGGTGGATCGCCGGCGAGAACCCGGAAACCTACCCGACCGGTCT
GTAGCGCAGAGTCCACGACG

Downstream 100 bases:

>100_bases
ACCAATCACACAAACCCCCATCGATCGTCGTCGGTATTGATGGCTCGAAGCCGGCCGTGCAAGCCGCACTGTGGGCGGTC
GACGAGGCAGCCAGCCGTGA

Product: putative phosphofructokinase pfkB

Products: ADP; D-fructose 1,6-bisphosphate

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTEPAAWDEGKPRIITLTMNPALDITTSVDVVRPTEKMRCGAPRYDPGGGGINVARIVHVLGGCSTALFPAGGSTGSLLM
ALLGDAGVPFRVIPIAASTRESFTVNESRTAKQYRFVLPGPSLTVAEQEQCLDELRGAAASAAFVVASGSLPPGVAADYY
QRVADICRRSSTPLILDTSGGGLQHISSGVFLLKASVRELRECVGSELLTEPEQLAAAHELIDRGRAEVVVVSLGSQGAL
LATRHASHRFSSIPMTAVSGVGAGDAMVAAITVGLSRGWSLIKSVRLGNAAGAAMLLTPGTAACNRDDVERFFELAAEPT
EVGQDQYVWHPIVNPEASP

Sequences:

>Translated_339_residues
MTEPAAWDEGKPRIITLTMNPALDITTSVDVVRPTEKMRCGAPRYDPGGGGINVARIVHVLGGCSTALFPAGGSTGSLLM
ALLGDAGVPFRVIPIAASTRESFTVNESRTAKQYRFVLPGPSLTVAEQEQCLDELRGAAASAAFVVASGSLPPGVAADYY
QRVADICRRSSTPLILDTSGGGLQHISSGVFLLKASVRELRECVGSELLTEPEQLAAAHELIDRGRAEVVVVSLGSQGAL
LATRHASHRFSSIPMTAVSGVGAGDAMVAAITVGLSRGWSLIKSVRLGNAAGAAMLLTPGTAACNRDDVERFFELAAEPT
EVGQDQYVWHPIVNPEASP
>Mature_338_residues
TEPAAWDEGKPRIITLTMNPALDITTSVDVVRPTEKMRCGAPRYDPGGGGINVARIVHVLGGCSTALFPAGGSTGSLLMA
LLGDAGVPFRVIPIAASTRESFTVNESRTAKQYRFVLPGPSLTVAEQEQCLDELRGAAASAAFVVASGSLPPGVAADYYQ
RVADICRRSSTPLILDTSGGGLQHISSGVFLLKASVRELRECVGSELLTEPEQLAAAHELIDRGRAEVVVVSLGSQGALL
ATRHASHRFSSIPMTAVSGVGAGDAMVAAITVGLSRGWSLIKSVRLGNAAGAAMLLTPGTAACNRDDVERFFELAAEPTE
VGQDQYVWHPIVNPEASP

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family

Homologues:

Organism=Escherichia coli, GI1788017, Length=304, Percent_Identity=41.1184210526316, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788493, Length=266, Percent_Identity=28.9473684210526, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PKKB_MYCTU (O86352)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842578
- PIR:   D70942
- RefSeq:   NP_216545.1
- RefSeq:   NP_336553.1
- ProteinModelPortal:   O86352
- SMR:   O86352
- EnsemblBacteria:   EBMYCT00000003751
- EnsemblBacteria:   EBMYCT00000069217
- GeneID:   887491
- GeneID:   924664
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2088
- KEGG:   mtu:Rv2029c
- TIGR:   MT2088
- TubercuList:   Rv2029c
- GeneTree:   EBGT00050000015542
- HOGENOM:   HBG670529
- OMA:   VYLVKPS
- ProtClustDB:   CLSK791574
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583
- TIGRFAMs:   TIGR03168

Pfam domain/function: PF00294 PfkB

EC number: 2.7.1.11

Molecular weight: Translated: 35402; Mature: 35271

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPAAWDEGKPRIITLTMNPALDITTSVDVVRPTEKMRCGAPRYDPGGGGINVARIVHV
CCCCCCCCCCCCEEEEEEECCCEEEECCCEEECCHHHHHCCCCCCCCCCCCHHHHHHHHH
LGGCSTALFPAGGSTGSLLMALLGDAGVPFRVIPIAASTRESFTVNESRTAKQYRFVLPG
HHCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCCCEEEEECC
PSLTVAEQEQCLDELRGAAASAAFVVASGSLPPGVAADYYQRVADICRRSSTPLILDTSG
CCEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
GGLQHISSGVFLLKASVRELRECVGSELLTEPEQLAAAHELIDRGRAEVVVVSLGSQGAL
CCHHHHHCCHHEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCE
LATRHASHRFSSIPMTAVSGVGAGDAMVAAITVGLSRGWSLIKSVRLGNAAGAAMLLTPG
EEEHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TAACNRDDVERFFELAAEPTEVGQDQYVWHPIVNPEASP
CCCCCHHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
TEPAAWDEGKPRIITLTMNPALDITTSVDVVRPTEKMRCGAPRYDPGGGGINVARIVHV
CCCCCCCCCCCEEEEEEECCCEEEECCCEEECCHHHHHCCCCCCCCCCCCHHHHHHHHH
LGGCSTALFPAGGSTGSLLMALLGDAGVPFRVIPIAASTRESFTVNESRTAKQYRFVLPG
HHCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCEEECCCCCCCCEEEEECC
PSLTVAEQEQCLDELRGAAASAAFVVASGSLPPGVAADYYQRVADICRRSSTPLILDTSG
CCEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCC
GGLQHISSGVFLLKASVRELRECVGSELLTEPEQLAAAHELIDRGRAEVVVVSLGSQGAL
CCHHHHHCCHHEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCE
LATRHASHRFSSIPMTAVSGVGAGDAMVAAITVGLSRGWSLIKSVRLGNAAGAAMLLTPG
EEEHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
TAACNRDDVERFFELAAEPTEVGQDQYVWHPIVNPEASP
CCCCCHHHHHHHHHHHCCHHHCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-fructose 6-phosphate

Specific reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036