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The map label for this gene is echA13 [H]

Identifier: 121637840

GI number: 121637840

Start: 2188363

End: 2189319

Strand: Reverse

Name: echA13 [H]

Synonym: BCG_1974c

Alternate gene names: 121637840

Gene position: 2189319-2188363 (Counterclockwise)

Preceding gene: 121637847

Following gene: 121637839

Centisome position: 50.05

GC content: 66.98

Gene sequence:

>957_bases
ATGTTCGTCGGCAGGGTCGGTCCGGTCGATCGCCGGTCGGACGGGGAACGCTCCCGACGGCCCCGGGAGTTCGAATACAT
CCGCTACGAAACCATCGACGATGGGCGCATCGCCGCGATCACCCTGGACCGCCCGAAACAACGCAACGCCCAGACCCGCG
GCATGCTGGTCGAGCTGGGCGCCGCCTTCGAACTTGCCGAGGCGGACGACACCGTCCGGGTGGTGATCCTGCGGGCCGCC
GGCCCCGCCTTCTCCGCCGGTCACGACCTCGGATCCGCTGACGATATCCGGGAACGCTCGCCCGGGCCGGACCAGCACCC
CAGCTACCGGTGCAACGGGGCGACCTTCGGCGGGGTCGAGTCACGCAACCGCCAGGAGTGGCACTACTACTTCGAAAACA
CCAAGCGGTGGCGCAACCTGCGCAAGATCACCATCGCCCAGGTGCACGGGGCCGTGCTGTCGGCGGGGTTGATGCTGGCC
TGGTGCTGCGATCTGATCGTCGCCAGCGAGGACACCGTGTTCGCCGATGTGGTCGGCACCCGGCTGGGCATGTGCGGGGT
CGAGTACTTCGGACATCCGTGGGAGTTCGGGCCGCGCAAGACCAAGGAACTGCTGCTCACCGGCGACTGCATAGGCGCCG
ACGAGGCCCACGCGCTGGGGATGGTCAGCAAGGTGTTTCCCGCTGACGAACTCGCGACCAGCACAATCGAATTCGCGCGT
CGGATCGCGAAGGTGCCGACGATGGCGGCACTGCTGATCAAGGAATCGGTGAACCAAACCGTCGATGCCATGGGGTTTTC
CGCCGCGCTGGACGGTTGCTTCAAGATCCACCAGCTCAATCACGCGCACTGGGGCGAAGTCACCGGCGGCAAGCTGTCCT
ACGGAACGGTCGAGTACGGCCTGGAGGACTGGCGCGCCGCACCGCAGATCCGGCCCGCGATCAAGCAGCGGCCCTGA

Upstream 100 bases:

>100_bases
TATATCGATCACTCTCATGTGCGTATTGGCTATGGACATGTTGGCTAAGGGACGGGTAACCTCGCACAGGTCAGCCGTTC
ACGTCCAGTAAGGAGCGGAC

Downstream 100 bases:

>100_bases
GGCCGGGGCGCGCAGTGGACGTCAGCTACCCACCCGAAGCGGAAGCGTTCCGCGACCGGATCCGGGAGTTTGTGGCCGAG
CACCTGCCGCCCGGGTGGCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 4-CBA-CoA dehalogenase-1; 4-CBCoA dehalogenase-1; 4-chlorobenzoyl-CoA dehalogenase-1 [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MFVGRVGPVDRRSDGERSRRPREFEYIRYETIDDGRIAAITLDRPKQRNAQTRGMLVELGAAFELAEADDTVRVVILRAA
GPAFSAGHDLGSADDIRERSPGPDQHPSYRCNGATFGGVESRNRQEWHYYFENTKRWRNLRKITIAQVHGAVLSAGLMLA
WCCDLIVASEDTVFADVVGTRLGMCGVEYFGHPWEFGPRKTKELLLTGDCIGADEAHALGMVSKVFPADELATSTIEFAR
RIAKVPTMAALLIKESVNQTVDAMGFSAALDGCFKIHQLNHAHWGEVTGGKLSYGTVEYGLEDWRAAPQIRPAIKQRP

Sequences:

>Translated_318_residues
MFVGRVGPVDRRSDGERSRRPREFEYIRYETIDDGRIAAITLDRPKQRNAQTRGMLVELGAAFELAEADDTVRVVILRAA
GPAFSAGHDLGSADDIRERSPGPDQHPSYRCNGATFGGVESRNRQEWHYYFENTKRWRNLRKITIAQVHGAVLSAGLMLA
WCCDLIVASEDTVFADVVGTRLGMCGVEYFGHPWEFGPRKTKELLLTGDCIGADEAHALGMVSKVFPADELATSTIEFAR
RIAKVPTMAALLIKESVNQTVDAMGFSAALDGCFKIHQLNHAHWGEVTGGKLSYGTVEYGLEDWRAAPQIRPAIKQRP
>Mature_318_residues
MFVGRVGPVDRRSDGERSRRPREFEYIRYETIDDGRIAAITLDRPKQRNAQTRGMLVELGAAFELAEADDTVRVVILRAA
GPAFSAGHDLGSADDIRERSPGPDQHPSYRCNGATFGGVESRNRQEWHYYFENTKRWRNLRKITIAQVHGAVLSAGLMLA
WCCDLIVASEDTVFADVVGTRLGMCGVEYFGHPWEFGPRKTKELLLTGDCIGADEAHALGMVSKVFPADELATSTIEFAR
RIAKVPTMAALLIKESVNQTVDAMGFSAALDGCFKIHQLNHAHWGEVTGGKLSYGTVEYGLEDWRAAPQIRPAIKQRP

Specific function: Dehalogenates 4-chlorobenzoyl-CoA, 4-iodobenzoyl-CoA, 4- bromobenzoyl-CoA and, at a slower rate, 4-fluorobenzoyl-CoA. Does not dehalogenate 2-chlorobenzoyl-CoA or 3-chlorobenzoyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=240, Percent_Identity=31.25, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI31542718, Length=234, Percent_Identity=32.0512820512821, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI260274832, Length=253, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI45643119, Length=253, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI260275230, Length=253, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787659, Length=228, Percent_Identity=26.7543859649123, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787660, Length=225, Percent_Identity=28.4444444444444, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=226, Percent_Identity=30.0884955752212, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=242, Percent_Identity=28.5123966942149, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=236, Percent_Identity=24.5762711864407, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=31.0204081632653, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=31.0204081632653, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357171, Length=242, Percent_Identity=29.3388429752066, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24654903, Length=219, Percent_Identity=25.5707762557078, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =3.8.1.7 [H]

Molecular weight: Translated: 35250; Mature: 35250

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVGRVGPVDRRSDGERSRRPREFEYIRYETIDDGRIAAITLDRPKQRNAQTRGMLVELG
CCCCCCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHCEEEEEC
AAFELAEADDTVRVVILRAAGPAFSAGHDLGSADDIRERSPGPDQHPSYRCNGATFGGVE
CCEEECCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCEECCCC
SRNRQEWHYYFENTKRWRNLRKITIAQVHGAVLSAGLMLAWCCDLIVASEDTVFADVVGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RLGMCGVEYFGHPWEFGPRKTKELLLTGDCIGADEAHALGMVSKVFPADELATSTIEFAR
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
RIAKVPTMAALLIKESVNQTVDAMGFSAALDGCFKIHQLNHAHWGEVTGGKLSYGTVEYG
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHC
LEDWRAAPQIRPAIKQRP
HHHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MFVGRVGPVDRRSDGERSRRPREFEYIRYETIDDGRIAAITLDRPKQRNAQTRGMLVELG
CCCCCCCCCCCCCCCHHHCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCHHHCEEEEEC
AAFELAEADDTVRVVILRAAGPAFSAGHDLGSADDIRERSPGPDQHPSYRCNGATFGGVE
CCEEECCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCEECCCC
SRNRQEWHYYFENTKRWRNLRKITIAQVHGAVLSAGLMLAWCCDLIVASEDTVFADVVGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RLGMCGVEYFGHPWEFGPRKTKELLLTGDCIGADEAHALGMVSKVFPADELATSTIEFAR
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
RIAKVPTMAALLIKESVNQTVDAMGFSAALDGCFKIHQLNHAHWGEVTGGKLSYGTVEYG
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCHHHHC
LEDWRAAPQIRPAIKQRP
HHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA