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The map label for this gene is gcvH

Identifier: 121637729

GI number: 121637729

Start: 2072963

End: 2073367

Strand: Direct

Name: gcvH

Synonym: BCG_1861

Alternate gene names: 121637729

Gene position: 2072963-2073367 (Clockwise)

Preceding gene: 121637728

Following gene: 121637730

Centisome position: 47.39

GC content: 61.73

Gene sequence:

>405_bases
GTGAGCGATATCCCGTCCGATCTGCACTACACCGCCGAACACGAGTGGATTCGCCGCAGTGGCGACGACACCGTCCGGGT
GGGGATCACCGACTATGCACAGTCGGCGCTTGGCGACGTCGTTTTCGTTCAGCTACCCGTTATCGGCACCGCGGTCACCG
CCGGCGAGACCTTCGGCGAAGTGGAATCGACGAAATCTGTGTCGGATCTCTATGCGCCCATTTCGGGTAAGGTGTCTGCG
GTCAACAGCGATCTGGACGGCACTCCGCAATTGGTGAATTCCGACCCCTACGGAGCCGGCTGGCTGCTGGACATCCAGGT
CGACAGCTCGGATGTCGCTGCCCTGGAGTCAGCTTTGACGACACTGCTCGACGCTGAGGCCTACCGCGGCACACTGACCG
AGTGA

Upstream 100 bases:

>100_bases
CGTGTGGACGTGACCGCCTTGCGGCAACCAAAACAGCACCAATACGCTCAGCCCGTCAAGTGAACTAGCCCAACTCCGAG
CCGACCAGAATAGGATTACC

Downstream 100 bases:

>100_bases
CGATTGCTAAGGTCCCTGCCAGCGTCACGTGGGAGGTCGCGGGTCTGCACGGATCCGGGCCGGGCAGGGCAATCGAGCCT
GGGATCCGCTGGGGTGCGCA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 133

Protein sequence:

>134_residues
MSDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGEVESTKSVSDLYAPISGKVSA
VNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALTTLLDAEAYRGTLTE

Sequences:

>Translated_134_residues
MSDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGEVESTKSVSDLYAPISGKVSA
VNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALTTLLDAEAYRGTLTE
>Mature_133_residues
SDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGEVESTKSVSDLYAPISGKVSAV
NSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALTTLLDAEAYRGTLTE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=121, Percent_Identity=40.495867768595, Blast_Score=93, Evalue=6e-20,
Organism=Homo sapiens, GI89057342, Length=121, Percent_Identity=39.6694214876033, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789271, Length=132, Percent_Identity=49.2424242424242, Blast_Score=136, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=45, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=125, Percent_Identity=40, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319272, Length=129, Percent_Identity=41.0852713178295, Blast_Score=110, Evalue=9e-26,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=42.8571428571429, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_MYCBO (Q7TZG8)

Other databases:

- EMBL:   BX248340
- RefSeq:   NP_855509.1
- ProteinModelPortal:   Q7TZG8
- SMR:   Q7TZG8
- EnsemblBacteria:   EBMYCT00000014455
- GeneID:   1092977
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1857
- GeneTree:   EBGT00050000015839
- HOGENOM:   HBG693789
- OMA:   SASEYEQ
- ProtClustDB:   PRK01202
- BioCyc:   MBOV233413:MB1857-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 14180; Mature: 14048

Theoretical pI: Translated: 3.76; Mature: 3.76

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGE
CCCCCCCCEECCHHHHHHCCCCCEEEEEEHHHHHHHHCCEEEEEECCCCEEEECCCCCCH
VESTKSVSDLYAPISGKVSAVNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALT
HHCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
TLLDAEAYRGTLTE
HHHHHHHHCCCCCC
>Mature Secondary Structure 
SDIPSDLHYTAEHEWIRRSGDDTVRVGITDYAQSALGDVVFVQLPVIGTAVTAGETFGE
CCCCCCCEECCHHHHHHCCCCCEEEEEEHHHHHHHHCCEEEEEECCCCEEEECCCCCCH
VESTKSVSDLYAPISGKVSAVNSDLDGTPQLVNSDPYGAGWLLDIQVDSSDVAALESALT
HHCCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHH
TLLDAEAYRGTLTE
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972